Acinetobacter quorum sensing contributes to inflammation-induced inhibition of orthopaedic implant osseointegration.

IF 3.2 3区医学 Q3 CELL & TISSUE ENGINEERING

European cells & materials Pub Date : 2022-06-09 DOI:10.22203/eCM.v043a18

H. Choe, BS Hausman, KM Hujer, O. Akkus, PN Rather, Z. Lee, R. Bonomo, E. Greenfield

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引用次数: 0

Abstract

Implant infection impairs osseointegration of orthopaedic implants by inducing inflammation. Acinetobacter spp. are increasingly prevalent multi-drug resistant bacteria that can cause osteomyelitis. Acinetobacter spp. can also cause inflammation and thereby inhibit osseointegration in mice. The purpose of the present study was to investigate the role of quorum sensing in this context. Therefore, wild-type bacteria were compared with an isogenic abaI mutant defective in quorum sensing in a murine osseointegration model. The abaI quorum- sensing mutant affected significantly less osseointegration and interleukin (IL) 1β levels, without detectably altering other pro-inflammatory cytokines. Wild-type bacteria had fewer effects on IL1 receptor (IL1R)-/- mice. These results indicated that quorum sensing in Acinetobacter spp. contributed to IL1β induction and the resultant inhibition of osseointegration in mice. Moreover, targeting the Gram-negative acyl-homoserine lactone quorum sensing may be particularly effective for patients with Acinetobacter spp. infections.

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不动杆菌群体感应有助于炎症诱导的骨科种植体骨整合抑制。

植入物感染通过诱导炎症损害骨科植入物的骨整合。不动杆菌是一种越来越普遍的可引起骨髓炎的耐多药细菌。不动杆菌也会引起炎症，从而抑制小鼠的骨整合。本研究的目的是调查群体感应在这方面的作用。因此，在小鼠骨整合模型中，将野生型细菌与群体感应缺陷的等基因abaI突变体进行了比较。abaI群体感应突变体显著降低骨整合和白细胞介素（IL）1β水平，而没有检测到其他促炎细胞因子的改变。野生型细菌对IL1受体（IL1R）-/-小鼠的影响较小。这些结果表明，不动杆菌的群体感应有助于IL1β的诱导和由此产生的对小鼠骨整合的抑制。此外，靶向革兰氏阴性酰基高丝氨酸内酯群体感应可能对不动杆菌感染患者特别有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European cells & materials 生物-材料科学：生物材料

CiteScore

6.00

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： eCM provides an interdisciplinary forum for publication of preclinical research in the musculoskeletal field (Trauma, Maxillofacial (including dental), Spine and Orthopaedics). The clinical relevance of the work must be briefly mentioned within the abstract, and in more detail in the paper. Poor abstracts which do not concisely cover the paper contents will not be sent for review. Incremental steps in research will not be entertained by eCM journal.Cross-disciplinary papers that go across our scope areas are welcomed.