European cells & materials最新文献_第2页

Microstructural adaptations of the subchondral bone are related to the mechanical axis deviation in end stage varus oa knees. 软骨下骨的微结构适应性与膝关节末期外翻的机械轴偏离有关。

IF 3.1 3区医学

European cells & materials Pub Date : 2023-02-20 DOI: 10.22203/eCM.v045a05

W Colyn, F Azari, J Bellemans, G H van Lenthe, L Scheys

{"title":"Microstructural adaptations of the subchondral bone are related to the mechanical axis deviation in end stage varus oa knees.","authors":"W Colyn, F Azari, J Bellemans, G H van Lenthe, L Scheys","doi":"10.22203/eCM.v045a05","DOIUrl":"10.22203/eCM.v045a05","url":null,"abstract":"Recent studies highlighted the crucial contribution of subchondral bone to OA development. Yet, only limited data have been reported on the relation between alteration to cartilage morphology, structural properties of the subchondral bone plate (SBP) and underlying subchondral trabecular bone (STB). Furthermore, the relationship between the morphometry of the cartilage and bone in the tibial plateau and the OA-induced changes in the joint's mechanical axis remains unexplored. Therefore, a visualisation and quantification of cartilage and subchondral bone microstructure in the medial tibial plateau was performed. End stage knee-OA patients with varus alignment and scheduled for total knee arthroplasty (TKA) underwent preoperative fulllength radiography to measure the hip-knee-ankle angle (HKA) and the mechanical-axis deviation (MAD). 18 tibial plateaux were μ-CT scanned (20.1 μm/voxel). Cartilage thickness, SBP, and STB microarchitecture were quantified in 10 volumes of interest (VOIs) in each medial tibial plateau. Significant differences (p < 0.001) were found for cartilage thickness, SBP, and STB microarchitecture parameters among the VOIs. Closer to the mechanical axis, cartilage thickness was consistently smaller, while SBP thickness and STB bone volume fraction (BV/TV) were higher. Moreover, trabeculae were also more superior-inferiorly oriented, i.e. perpendicular to the transverse plane of the tibial plateau. As cartilage and subchondral bone changes reflect responses to local mechanical loading patterns in the joint, the results suggested that region-specific subchondral bone adaptations were related to the degree of varus deformity. More specifically, subchondral sclerosis appeared to be most pronounced closer to the mechanical axis of the knee.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"45 ","pages":"60-71"},"PeriodicalIF":3.1,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9114957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mandibular condylar process remodeling in rats with different bite-altering devices. 不同咬合改变装置对大鼠下颌髁突重塑的影响。

IF 3.1 3区医学

European cells & materials Pub Date : 2023-02-14 DOI: 10.22203/eCM.v045a04

W Li, S Trbojevic, J B Pineda-Farias, X Liu, M S Gold, A J Almarza

{"title":"Mandibular condylar process remodeling in rats with different bite-altering devices.","authors":"W Li, S Trbojevic, J B Pineda-Farias, X Liu, M S Gold, A J Almarza","doi":"10.22203/eCM.v045a04","DOIUrl":"https://doi.org/10.22203/eCM.v045a04","url":null,"abstract":"The objective was to compare different dental splint models and materials for inducing abnormal loading on the gross morphology and histological appearance of the mandibular condylar processes of Sprague Dawley rats. Three different types of dental splints (resin molar, aluminum incisor, stainless-steel incisor) were placed unilaterally to induce occlusal perturbation for 4 weeks. At that time, mandibular condylar processes were assessed by gross appearance and histology. Quantitative measurements were also conducted on the hematoxylin and eosin images for condyle shape. The results showed that although the condylar cartilage was affected by all splint types, the resin molar splint was associated with the most extensive mandibular condylar process remodeling, which was primarily a slant (skewness) of the lateral aspect of the condylar process. Additionally, quantitative measurements on the histological specimens demonstrated that the split and tilt angle of the left (ipsilateral) condylar processes in the resin molar group (124.8 ± 12.7° and 104.1 ± 12.7°, respectively) increased significantly (p < 0.05) when compared to right (contralateral) condylar processes (104.7 ± 5.8°and 91.6 ± 4.4°, respectively). However, no changes were noted on the thickness of the fibrocartilage layer at medial, central, and lateral regions of the condylar process. Another major finding is the high variability of morphology of the naïve animals. Future studies will assess the impact of longer durations of splinting, age, and sex on the remodeling of the mandibular condylar process, allowing for the development of diagnostics and therapies.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"45 ","pages":"46-59"},"PeriodicalIF":3.1,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis. 单轴循环拉伸通过YAP-Wnt/β-catenin轴促进opll来源的原代细胞成骨分化。

IF 3.1 3区医学

European cells & materials Pub Date : 2023-02-07 DOI: 10.22203/eCM.v045a03

Z Zhu, T Tang, Z He, F Wang, H Chen, G Chen, J Zhou, S Liu, J Wang, W Tian, D Chen, X Wu, X Liu, Z Zhou, S Liu

{"title":"Uniaxial cyclic stretch enhances osteogenic differentiation of OPLL-derived primary cells via YAP-Wnt/β-catenin axis.","authors":"Z Zhu, T Tang, Z He, F Wang, H Chen, G Chen, J Zhou, S Liu, J Wang, W Tian, D Chen, X Wu, X Liu, Z Zhou, S Liu","doi":"10.22203/eCM.v045a03","DOIUrl":"https://doi.org/10.22203/eCM.v045a03","url":null,"abstract":"The pathogenesis of posterior longitudinal ligament ossification (OPLL) remains inadequately understood. Mechanical stimulation is one of the important pathogenic factors in OPLL. As one of the mechanical stimulation transduction signals, the yes-associated protein (YAP) interacts with the Wnt/β-catenin signalling pathway, which plays an important role in osteogenic differentiation. This study aimed to demonstrate the role of YAP-Wnt/β-catenin axis in cell differentiation induced by mechanical stress. Primary cells extracted from posterior longitudinal ligament tissues from OPLL or non-OPLL patients were subjected to sinusoidal uniaxial cyclic stretch (5 %, 0.5 Hz, 3 d). The expression of runt-related transcription factor 2, collagen I, osterix, osteocalcin and alkaline phosphatase were compared between the static and the experimental groups. In addition, the cytoskeleton was detected using phalloidin staining while YAP phosphorylation states and nuclear location were identified using immunofluorescence. The results showed that mechanical stretching loading increased the expression of osteogenic genes and proteins in the OPLL group, while it had no significant effect on the control group. When OPLL cells were stretched, YAP exhibited an obvious nuclear translocation and the Wnt/β-catenin pathway was activated. Knocking down YAP or β-catenin could weaken the impact upon osteogenic differentiation induced by mechanical stimulation. YAP-mediated mechanical stimulation promoted osteogenic differentiation of OPLL cells through Wnt/β-catenin pathway and this progress was independent of the Hippo pathway.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"45 ","pages":"31-45"},"PeriodicalIF":3.1,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9126475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

GRP78 promotes the osteogenic and angiogenic response in periodontal ligament stem cells. GRP78 可促进牙周韧带干细胞的成骨和血管生成反应。

IF 3.1 3区医学

European cells & materials Pub Date : 2023-01-23 DOI: 10.22203/eCM.v045a02

A Merkel, Y Chen, C Villani, A George

{"title":"GRP78 promotes the osteogenic and angiogenic response in periodontal ligament stem cells.","authors":"A Merkel, Y Chen, C Villani, A George","doi":"10.22203/eCM.v045a02","DOIUrl":"10.22203/eCM.v045a02","url":null,"abstract":"Periodontitis is a progressive disease that ultimately leads to bone and tooth loss. A major consequence of periodontal disease is the inability to regain lost bone in the periodontium. The importance was demonstrated of glucose-regulated protein-78 (GRP78) in the osteogenic differentiation of periodontal ligament stem cells and their potential use for regeneration of the periodontium. Previous studies have shown the relationship between GRP78 and dentine matrix protein-1 (DMP1). The importance of this receptor-ligand complex in supporting the process of osteogenesis and angiogenesis was confirmed in this study. To show the function of GRP78 in mineralised tissues, transgenic periodontal ligament stem cells (PDLSCs) were generated in which GRP78 was either overexpressed or silenced. Gene expression analysis of the cells cultured under osteogenic conditions showed an increase in key osteogenic genes with the overexpression of GRP78. RNA-Seq analysis was also performed to understand the transcriptome profile associated with genotype changes. Using the database for annotation, visualisation, and integration discovery (DAVID) for the functional enrichment analysis of differentially expressed genes, the upregulation of genes promoting osteogenesis and angiogenesis with GRP78 overexpression was demonstrated. Alizarin red staining and scanning electron microscopy analysis revealed matrix mineralisation with increased calcium deposition in GRP78 overexpressing cells. The in vivo osteogenic and angiogenic function of GRP78 was shown using a subcutaneous implantation rodent model. The results suggested that GRP78 in PDLSCs can regulate the expression of both osteogenesis and angiogenesis. Therefore, GRP78 could be considered as a therapeutic target for repair of diseased periodontium.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"45 ","pages":"14-30"},"PeriodicalIF":3.1,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10243372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of temporomandibular joint articular disc progenitor cell clones. 颞下颌关节关节盘祖细胞克隆的表征。

IF 3.1 3区医学

European cells & materials Pub Date : 2023-01-09 DOI: 10.22203/eCM.v045a01

K J Weekes, P Lam, C Kim, B Johnstone

{"title":"Characterization of temporomandibular joint articular disc progenitor cell clones.","authors":"K J Weekes, P Lam, C Kim, B Johnstone","doi":"10.22203/eCM.v045a01","DOIUrl":"https://doi.org/10.22203/eCM.v045a01","url":null,"abstract":"A critical component of the temporomandibular joint (TMJ) is the fibrocartilage articular disc (AD). Researchers have attempted to regenerate the AD to alleviate TMJ osteoarthritis but alternative cell sources for use in AD regenerative approaches are needed due to insufficient extracellular matrix (ECM) production by total articular disc cells (TACs). Tissue-specific progenitor cells have been identified in many tissues. The aim of the present study was to identify adult multipotent progenitor cells within the AD suitable for regenerative medicine applications. A novel AD progenitor cell population was identified in rhesus macaques. Clonally derived articular disc progenitor cells (ADPs) were isolated using fibronectin differential cell adhesion. ADPs represent between 1 and 3 % of the TAC population and are capable of in vitro expansion beyond 60 population doublings. ADPs were characterized using osteogenic, adipogenic, and fibrochondrogenesis differentiation assays. Clones exhibited phenotypic plasticity, differentiating into osteocytes, adipocytes, and fibrochondrocytes. ECM secretion profiles following fibrochondrogenic differentiation were assessed using immunohistochemistry (IHC), fluorescently activated cell sorting (FACS), total collagen, and glycosaminoglycan (GAG) assays and compared with TACs, articular cartilage progenitor cells (ACPs), tendon progenitor cells (TPCs) and bone-marrow-derived mesenchymal stem cells (BMMSCs). ADP pellet cultures produced a biochemical phenotype similar to native AD tissue, with production of versican (VCAN) and collagen types I, II, III, and VI (COL1, COL2, COL3, COL6). However, clonally derived ADP cell lines produced different amounts of ECM and exhibited different expansion potentials. These findings indicated flexibility in clone selection for potential regenerative strategies to recapitulate native anisotropy.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"45 ","pages":"1-13"},"PeriodicalIF":3.1,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9119089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal murine skeletogenesis partially recovers from absent skeletal muscle as development progresses. 随着发育的进展，产前小鼠骨骼生成部分从缺失的骨骼肌中恢复。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-11-08 DOI: 10.22203/eCM.v044a08

V Sotiriou, Y Huang, S Ahmed, H Isaksson, N C Nowlan

{"title":"Prenatal murine skeletogenesis partially recovers from absent skeletal muscle as development progresses.","authors":"V Sotiriou, Y Huang, S Ahmed, H Isaksson, N C Nowlan","doi":"10.22203/eCM.v044a08","DOIUrl":"https://doi.org/10.22203/eCM.v044a08","url":null,"abstract":"Skeletal muscle contractions are critical for normal skeletal growth and morphogenesis but it is unclear how the detrimental effects of absent muscle on the bones and joints change over time. Joint shape and cavitation as well as rudiment length and mineralisation were assessed in multiple rudiments at two developmental stages [Theiler stage (TS)24 and TS27] in the splotch-delayed \"muscle-less limb\" mouse model and littermate controls. Chondrocyte morphology was quantified in 3D in the distal humerus at the same stages. As development progressed, the effects of absent muscle on all parameters except for cavitation become less severe. All major joints in muscle-less limbs were abnormally shaped at TS24, while, by TS27, most muscle-less limb joint shapes were normal or nearly normal. In contrast, any joints that were fused at TS24 did not cavitate by TS27. At TS24, chondrocytes in the distal humerus were significantly smaller in the muscle-less limbs than in controls, while by TS27, chondrocyte volume was similar between the two groups, offering a cell-level mechanism for the partial recovery in shape of muscle-less limbs. Mineralisation showed the most pronounced changes over gestation. At TS24, all muscle-less rudiments studied had less mineralisation than the controls, while at TS27, muscle-less limb rudiments had mineralisation extents equivalent to controls. In conclusion, the effects of muscle absence on prenatal murine skeletogenesis reduced in severity over gestation. Understanding how mammalian bones and joints continue to develop in an environment with abnormal fetal movements provides insights into conditions including hip dysplasia and arthrogryposis.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"44 ","pages":"115-132"},"PeriodicalIF":3.1,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10389450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF2 overrides key pro-fibrotic features of bone marrow stromal cells isolated from Modic type 1 change patients. FGF2覆盖从Modic 1型改变患者中分离的骨髓基质细胞的关键促纤维化特征。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-10-18 DOI: 10.22203/eCM.v044a07

I Heggli, U Blache, N Herger, T Mengis, P K Jaeger, R Schuepbach, N Farshad-Amacker, F Brunner, J G Snedeker, M Farshad, O Distler, S Dudli

{"title":"FGF2 overrides key pro-fibrotic features of bone marrow stromal cells isolated from Modic type 1 change patients.","authors":"I Heggli, U Blache, N Herger, T Mengis, P K Jaeger, R Schuepbach, N Farshad-Amacker, F Brunner, J G Snedeker, M Farshad, O Distler, S Dudli","doi":"10.22203/eCM.v044a07","DOIUrl":"https://doi.org/10.22203/eCM.v044a07","url":null,"abstract":"Extensive extracellular matrix production and increased cell-matrix adhesion by bone marrow stromal cells (BMSCs) are hallmarks of fibrotic alterations in the vertebral bone marrow known as Modic type 1 changes (MC1). MC1 are associated with non-specific chronic low-back pain. To identify treatment targets for MC1, in vitro studies using patient BMSCs are important to reveal pathological mechanisms. For the culture of BMSCs, fibroblast growth factor 2 (FGF2) is widely used. However, FGF2 has been shown to suppress matrix synthesis in various stromal cell populations. The aim of the present study was to investigate whether FGF2 affected the in vitro study of the fibrotic pathomechanisms of MC1-derived BMSCs. Transcriptomic changes and changes in cell-matrix adhesion of MC1-derived BMSCs were compared to intra-patient control BMSCs in response to FGF2. RNA sequencing and quantitative real-time polymerase chain reaction revealed that pro-fibrotic genes and pathways were not detectable in MC1-derived BMSCs when cultured in the presence of FGF2. In addition, significantly increased cell-matrix adhesion of MC1-derived BMSCs was abolished in the presence of FGF2. In conclusion, the data demonstrated that FGF2 overrides key pro-fibrotic features of MC1 BMSCs in vitro. Usage of FGF2-supplemented media in studies of fibrotic mechanisms should be critically evaluated as it could override normally dominant biological and biophysical cues.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"44 ","pages":"101-114"},"PeriodicalIF":3.1,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10401744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Physiological stretching induces a differential extracellular matrix gene expression response in acetabular labrum cells. 生理拉伸诱导髋臼唇细胞细胞外基质差异基因表达反应。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-10-03 DOI: 10.22203/eCM.v044a06

S Huber, S Günther, E Cambria, M Leunig, S J Ferguson

{"title":"Physiological stretching induces a differential extracellular matrix gene expression response in acetabular labrum cells.","authors":"S Huber, S Günther, E Cambria, M Leunig, S J Ferguson","doi":"10.22203/eCM.v044a06","DOIUrl":"https://doi.org/10.22203/eCM.v044a06","url":null,"abstract":"The acetabular labrum is a fibrocartilaginous ring surrounding the acetabulum and is important for hip stability and contact pressure dissipation through a sealing function. Injury of the labrum may contribute to hip-joint degeneration and development of secondary osteoarthritis. Understanding how extracellular matrix (ECM) production and remodelling is regulated is of key importance for successful tissue restoration. The present study hypothesised that physiological stretching enhanced the metabolic activity and altered the ECM gene expression in labrum cells. Primary bovine labrum cells were physiologically stretched for up to 5 d. 24 h after the last stretch cycle, changes in metabolic activity were measured using the PrestoBlue™ HS Cell Viability Reagent and ECM gene expression was examined using the quantitative polymerase chain reaction method. Targets of interest were further investigated using immunofluorescence and enzyme-linked immunosorbent assay. Metabolic activity was not affected by the stretching (0.9746 ± 0.0614, p > 0.05). Physiological stretching upregulated decorin (DCN) (1.8548 ± 0.4883, p = 0.002) as well as proteoglycan 4 (PRG4) (1.7714 ± 0.6600, p = 0.029) and downregulated biglycan (BGN) (0.7018 + 0.1567, p = 0.008), cartilage oligomeric matrix protein (COMP) (0.5747 ± 0.2650, p = 0.029), fibronectin (FN1) (0.5832 ± 0.0996, p < 0.001) and spondin 1 (SPON1) (0.6282 ± 0.3624, p = 0.044) gene expression. No difference in PRG4 and DCN abundance or release could be measured. The here identified mechanosensitive targets are known to play relevant roles in tissue organisation. Therefore, physiological stretching might play a role in labrum tissue homeostasis and regeneration.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":" ","pages":"90-100"},"PeriodicalIF":3.1,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40393635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Osteochondral explants for diarthrodial joint diseases: bridging the gap between bench and bedside. 骨软骨外植体治疗腹泻性关节疾病:弥合临床与临床之间的差距。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-09-26 DOI: 10.22203/eCM.v044a05

K H Li, Y Zhu, P H Zhang, M Alini, S Grad, Z Li

引用次数: 2

Perspectives on in silico bone mechanobiology: computational modelling of multicellular systems. 硅骨力学生物学的观点:多细胞系统的计算建模。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-08-30 DOI: 10.22203/eCM.v044a04

D Boaretti, E Wehrle, Y D Bansod, D C Tourolle Né Betts, R Müller

{"title":"Perspectives on in silico bone mechanobiology: computational modelling of multicellular systems.","authors":"D Boaretti, E Wehrle, Y D Bansod, D C Tourolle Né Betts, R Müller","doi":"10.22203/eCM.v044a04","DOIUrl":"https://doi.org/10.22203/eCM.v044a04","url":null,"abstract":"Bone mechanobiology is the study of the physical, biological and mechanical processes that continuously affect the multiscale multicellular system of the bone from the organ to the molecular scale. Current knowledge derives from experimental studies, which are often limited to gathering qualitative data in a cross-sectional manner, up to a restricted number of time points. Moreover, the simultaneous collection of information about 3D bone microarchitecture, cell activity as well as protein distribution and level is still a challenge. In silico models can expand qualitative information with hypothetical quantitative systems, which allow quantification, testing and comparison to existing quantifiable experimental data. An overview of multiscale, multiphysics, agent-based and hybrid techniques and their applications to bone mechanobiology is provided in the present review. The study analysed how mechanical signals, cells and proteins can be modelled in silico to represent bone remodelling and adaptation. Hybrid modelling of bone mechanobiology could combine the methods used in multiscale, multiphysics and agent-based models into a single model, leading to a unified and comprehensive understanding of bone mechanobiology. Numerical simulations of in vivo multicellular systems aided in hypothesis testing of such in silico models. Recently, in silico trials have been used to illustrate the mechanobiology of cells and signalling pathways in clinical biopsies and animal bones, including the effects of drugs on single cells and signalling pathways up to the organ level. This improved understanding may lead to the identification of novel therapies for degenerative diseases such as osteoporosis.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":" ","pages":"56-73"},"PeriodicalIF":3.1,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33447315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4