European cells & materials最新文献_第4页

Bioactive molecules for regenerative pulp capping. 再生髓盖生物活性分子。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-29 DOI: 10.22203/eCM.v042a26

L L Whitehouse, N H Thomson, T Do, G A Feichtinger

{"title":"Bioactive molecules for regenerative pulp capping.","authors":"L L Whitehouse, N H Thomson, T Do, G A Feichtinger","doi":"10.22203/eCM.v042a26","DOIUrl":"https://doi.org/10.22203/eCM.v042a26","url":null,"abstract":"Since the discovery of bioactive molecules sequestered in dentine, researchers have been exploring ways to harness their activities for dental regeneration. One specific area, discussed in this review, is that of dental-pulp capping. Dental-pulp caps are placed when the dental pulp is exposed due to decay or trauma in an attempt to enhance tertiary dentine deposition. Several materials are used for dental-pulp capping; however, natural biomimetic scaffolds may offer advantages over manufactured materials such as improved aesthetic, biocompatibility and success rate. The present review discusses and appraises the current evidence surrounding biomimetic dental-pulp capping, with a focus on bioactive molecules sequestered in dentine. Molecules covered most extensively in the literature include transforming growth factors (TGF-βs, specifically TGF-β1) and bone morphogenetic proteins (BMPs, specifically BMP-2 and BMP-7). Further studies would need to explore the synergistic use of multiple peptides together with the development of a tailored scaffold carrier. The roles of some of the molecules identified in dentine need to be explored before they can be considered as potential bioactive molecules in a biomimetic scaffold for dental-pulp capping. Future in vivo work needs to consider the inflammatory environment of the dental pulp in pulpal exposures and compare pulp-capping materials.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"415-437"},"PeriodicalIF":3.1,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39787802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Vertebral osteomyelitis is characterised by increased RANK/OPG and RANKL/OPG expression ratios in vertebral bodies and intervertebral discs. 椎体骨髓炎的特征是椎体和椎间盘中RANK/OPG和RANKL/OPG表达比升高。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-29 DOI: 10.22203/eCM.v042a27

S Lang, M Loibl, J Gläsner, M Simon, M Rupp, S Grad, C Neumann, V Alt, A Gessner, F Hanses

{"title":"Vertebral osteomyelitis is characterised by increased RANK/OPG and RANKL/OPG expression ratios in vertebral bodies and intervertebral discs.","authors":"S Lang, M Loibl, J Gläsner, M Simon, M Rupp, S Grad, C Neumann, V Alt, A Gessner, F Hanses","doi":"10.22203/eCM.v042a27","DOIUrl":"https://doi.org/10.22203/eCM.v042a27","url":null,"abstract":"Vertebral osteomyelitis (VO) is an infection of the spine mainly caused by bacterial pathogens. The pathogenesis leading to destruction of intervertebral discs (IVDs) and adjacent vertebral bodies (VBs) is poorly described. The present study aimed at investigating the connection between infection and bone/disc metabolism in VO patients. 14 patients with VO (infection group) and 14 patients with burst fractures of the spine (fracture group; control) were included prospectively. Tissue biopsies from affected IVDs and adjacent VBs were analysed by RT-qPCR for mRNA-expression levels of 18 target genes including chemokines, adipokines and genes involved in bone metabolism. Most importantly, the receptor activator of NF-κB/osteoprotegerin (RANK/OPG) expression ratio was drastically elevated in both VBs and IVDs of the infection group. In parallel, expression of genes of the prostaglandin-E2-dependent prostanoid system was induced. Such genes regulate tissue degradation processes via the triad OPG/RANK/RANKL as well as via the chemokines IL-8 and CCL-20, whose expression was also found to be increased upon infection. The gene expression of the adipokine leptin, which promotes inflammatory tissue degradation, was higher in IVD tissue of the infection group, whereas the transcription of omentin and resistin genes, whose functions are largely unknown in the context of infectious diseases, was lower in infected VBs. In summary, similar expression patterns of pro-inflammatory cytokines and pro-osteoclastogenic factors were identified in VBs and IVDs of patients suffering from VO. This suggests that common immuno-metabolic pathways are involved in the mechanisms leading to tissue degradation in VBs and IVDs during VO.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"438-451"},"PeriodicalIF":3.1,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39945894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells. TWIST1控制间充质干细胞的细胞衰老和能量代谢。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-25 DOI: 10.22203/eCM.v042a25

C Voskamp, L A Anderson, W J Koevoet, S Barnhoorn, P G Mastroberardino, G J van Osch, R Narcisi

{"title":"TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells.","authors":"C Voskamp, L A Anderson, W J Koevoet, S Barnhoorn, P G Mastroberardino, G J van Osch, R Narcisi","doi":"10.22203/eCM.v042a25","DOIUrl":"https://doi.org/10.22203/eCM.v042a25","url":null,"abstract":"Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Since the transcription factor TWIST1 influences expansion of MSCs, its role in regulating cellular senescence was investigated. The present study demonstrated that silencing of TWIST1 in MSCs increased the occurrence of senescence, characterised by a SASP profile different from irradiation-induced senescent MSCs. Knowing that senescence alters cellular metabolism, cellular bioenergetics was monitored by using the Seahorse XF apparatus. Both TWIST1-silencing-induced and irradiation-induced senescent MSCs had a higher oxygen consumption rate compared to control MSCs, while TWIST1-silencing-induced senescent MSCs had a low extracellular acidification rate compared to irradiation-induced senescent MSCs. Overall, data indicated how TWIST1 regulation influenced senescence in MSCs and that TWIST1 silencing-induced senescence was characterised by a specific SASP profile and metabolic state.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"401-414"},"PeriodicalIF":3.1,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39660723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dentine matrix metalloproteinases as potential mediators of dentine regeneration. 牙本质基质金属蛋白酶是牙本质再生的潜在介质。

IF 3.2 3区医学

European cells & materials Pub Date : 2021-11-24 DOI: 10.22203/eCM.v042a24

E Guirado, A George

引用次数: 0

Tendon mechanobiology in small-animal experiments during post-transection healing. 肌腱力学生物学在小动物实验中的应用。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-02 DOI: 10.22203/eCM.v042a23

T Notermans, H Hammerman, P Eliasson, H Isaksson

引用次数: 2

Current osteomyelitis mouse models, a systematic review. 目前骨髓炎小鼠模型，系统综述。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-21 DOI: 10.22203/eCM.v042a22

C Guarch-Pérez, M Riool, S Aj Zaat

{"title":"Current osteomyelitis mouse models, a systematic review.","authors":"C Guarch-Pérez, M Riool, S Aj Zaat","doi":"10.22203/eCM.v042a22","DOIUrl":"https://doi.org/10.22203/eCM.v042a22","url":null,"abstract":"Osteomyelitis is an inflammatory bone disease caused by an infecting microorganism leading to a gradual bone loss. Due to the difficulty in studying osteomyelitis directly in patients, animal models allow researchers to investigate the pathogenesis of the infection and the development of novel prophylactic, anti-inflammatory and antimicrobial treatment strategies. This review is specifically focused on the in vivo mouse osteomyelitis studies available in literature. Thus, a systematic search on Web of Science and PubMed was conducted using the query \"(infection) AND (mice OR mouse OR murine) AND (model OR models) AND (arthroplasty OR fracture OR (internal fixator) OR (internal fixation OR prosthesis OR implant OR osteomyelitis)\". After critical assessment of the studies according to the inclusion and exclusion criteria, 135 studies were included in the detailed analysis. Based on the model characteristics, the studies were classified into five subject groups: haematogenous osteomyelitis, post-traumatic osteomyelitis, bone-implant-related infection, peri-prosthetic joint infection, fracture-related infection. In addition, the characteristics of the mice used, such as inbred strain, age or gender, the characteristics of the pathogens used, the inoculation methods, the type of anaesthesia and analgesia used during surgery and the procedures for evaluating the pathogenicity of the infecting micro-organism were described. Overall, the mouse is an excellent first step in vivo model to study the pathogenesis, inflammation and healing process of osteomyelitis and to evaluate novel prophylaxis and treatment strategies.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"334-374"},"PeriodicalIF":3.1,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39538575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Bone infection: a clinical priority for clinicians, scientists and educators. 骨感染:临床医生，科学家和教育工作者的临床优先事项。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-18 DOI: 10.22203/eCM.v042a21

T F Moriarty, G Muthukrishnan, J L Daiss, C Xie, K Nishitani, Y Morita, H Awad, K L de Mesy Bentley, E Masters, T Bui, M Yan, J Owen, B Mooney, S Gill, J Puetzler, J C Wenke, M Morgenstern, W-J Metsemakers, C Noll, A Joeris, R G Richards, E M Schwarz, S L Kates

{"title":"Bone infection: a clinical priority for clinicians, scientists and educators.","authors":"T F Moriarty, G Muthukrishnan, J L Daiss, C Xie, K Nishitani, Y Morita, H Awad, K L de Mesy Bentley, E Masters, T Bui, M Yan, J Owen, B Mooney, S Gill, J Puetzler, J C Wenke, M Morgenstern, W-J Metsemakers, C Noll, A Joeris, R G Richards, E M Schwarz, S L Kates","doi":"10.22203/eCM.v042a21","DOIUrl":"https://doi.org/10.22203/eCM.v042a21","url":null,"abstract":"Bone infection has received increasing attention in recent years as one of the main outstanding clinical problems in orthopaedic-trauma surgery that has not been successfully addressed. In fact, infection may develop across a spectrum of patient types regardless of the level of perioperative management, including antibiotic prophylaxis. Some of the main unknown factors that may be involved, and the main targets for future intervention, include more accurate and less invasive diagnostic options, more thorough and accurate debridement protocols, and more potent and targeted antimicrobials. The underlying biology dominates the clinical management of bone infections, with features such as biofilm formation, osteolysis and vascularisation being particularly influential. Based on the persistence of this problem, an improved understanding of the basic biology is deemed necessary to enable innovation in the field. Furthermore, from the clinical side, better evidence, documentation and outreach will be required to translate these innovations to the patient. This review presents the findings and progress of the AO Trauma Clinical Priority Program on the topic of bone infection.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"312-333"},"PeriodicalIF":3.1,"publicationDate":"2021-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39528870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pulsed ultrasound for bone regeneration - outcomes and hurdles in the clinical application: a systematic review. 脉冲超声骨再生-结果和障碍在临床应用:系统回顾。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-14 DOI: 10.22203/eCM.v042a20

R Puts, R Vico, N Beilfuß, M Shaka, F Padilla, K Raum

{"title":"Pulsed ultrasound for bone regeneration - outcomes and hurdles in the clinical application: a systematic review.","authors":"R Puts, R Vico, N Beilfuß, M Shaka, F Padilla, K Raum","doi":"10.22203/eCM.v042a20","DOIUrl":"https://doi.org/10.22203/eCM.v042a20","url":null,"abstract":"Impaired bone-fracture healing is associated with long-term musculoskeletal disability, pain and psychological distress. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive and side-effect-free treatment option for fresh, delayed- and non-union bone fractures, which has been used in patients since the early 1990s. Several clinical studies, however, have questioned the usefulness of the LIPUS treatment for the regeneration of long bones, including those with a compromised healing. This systematic review addresses the hurdles that the clinical application of LIPUS encounters. Low patient compliance might disguise the effects of the LIPUS therapy, as observed in several studies. Furthermore, large discrepancies in results, showing profound LIPUS effects in regeneration of small-animal bones in comparison to the clinical studies, could be caused by the suboptimal parameters of the clinical set-up. This raises the question of whether the so-called \"acoustic dose\" requires a thorough characterisation to reveal the mechanisms of the therapy. The adequate definition of the acoustic dose is especially important in the elderly population and patients with underlying medical conditions, where distinct biological signatures lead to a delayed regeneration. Non-industry-funded, randomised, double-blind, placebo-controlled clinical trials of the LIPUS application alone and as an adjuvant treatment for bones with complicated healing, where consistent control of patient compliance is ensured, are required.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"281-311"},"PeriodicalIF":3.1,"publicationDate":"2021-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39516660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Bone loss recovery in mice following microgravity with concurrent bone-compartment-specific osteocyte characteristics. 微重力后小鼠骨丢失恢复与骨室特异性骨细胞特征。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-13 DOI: 10.22203/eCM.v042a16

S von Kroge, E M Wölfel, L B Buravkova, D A Atiakshin, E A Markina, T Schinke, T Rolvien, B Busse, K Jähn-Rickert

{"title":"Bone loss recovery in mice following microgravity with concurrent bone-compartment-specific osteocyte characteristics.","authors":"S von Kroge, E M Wölfel, L B Buravkova, D A Atiakshin, E A Markina, T Schinke, T Rolvien, B Busse, K Jähn-Rickert","doi":"10.22203/eCM.v042a16","DOIUrl":"https://doi.org/10.22203/eCM.v042a16","url":null,"abstract":"Space missions provide the opportunity to investigate the influence of gravity on the dynamic remodelling processes in bone. Mice were examined following space flight and subsequent recovery to determine the effects on bone compartment-specific microstructure and composition. The resulting bone loss following microgravity recovered only in trabecular bone, while in cortical bone the tissue mineral density was restored after only one week on Earth. Detection of TRAP-positive bone surface cells in the trabecular compartment indicated increased resorption following space flight. In cortical bone, a persistent reduced viability of osteocytes suggested an impaired sensitivity to mechanical stresses. A compartment-dependent structural recovery from microgravity-induced bone loss was shown, with a direct osteocytic contribution to persistent low bone volume in the cortical region even after a recovery period. Trabecular recovery was not accompanied by changes in osteocyte characteristics. These post-space-flight findings will contribute to the understanding of compositional changes that compromise bone quality caused by unloading, immobilisation, or disuse.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"220-231"},"PeriodicalIF":3.1,"publicationDate":"2021-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39514236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

A bilayered tissue engineered in vitro model simulating the tooth periodontium. 模拟牙周组织的双层组织工程体外模型。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-11 DOI: 10.22203/eCM.v042a17

A Khadre, E Lm Raif, S Junaid, O M Goudouri, W Refaat, A Ramadan, A R Boccaccini, R El-Gendy

{"title":"A bilayered tissue engineered in vitro model simulating the tooth periodontium.","authors":"A Khadre, E Lm Raif, S Junaid, O M Goudouri, W Refaat, A Ramadan, A R Boccaccini, R El-Gendy","doi":"10.22203/eCM.v042a17","DOIUrl":"https://doi.org/10.22203/eCM.v042a17","url":null,"abstract":"Due to the complexity of the structure of the tooth periodontium, regeneration of the full tooth attachment is not a trivial task. There is also a gap in models that can represent human tooth attachment in vitro and in vivo. The aim of this study was to develop a bilayered in vitro construct that simulated the tooth periodontal ligament and attached alveolar bone, for the purpose of tissue regeneration and investigation of physiological and orthodontic loading. Two types of materials were used to develop this construct: sol-gel 60S10Mg derived scaffold, representing the hard tissue component of the periodontium, and commercially available Geistlich Bio-Gide® collagen membrane, representing the soft tissue component of the tooth attachment. Each scaffold was dynamically seeded with human periodontal ligament cells (HPDLCs). Scaffolds were either cultured separately, or combined in a bilayered construct, for 2 weeks. Characterisation of the individual scaffolds and the bilayered constructs included biological characterisation (cell viability, scanning electron microscopy to confirm cell attachment, gene expression of periodontium regeneration markers), and mechanical characterisation of scaffolds and constructs. HPDLCs enjoyed a biocompatible 3-dimensional environment within the bilayered construct components. There was no drop in cellular gene expression in the bilayered construct, compared to the separate scaffolds.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"232-245"},"PeriodicalIF":3.1,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39507059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1