European cells & materials最新文献_第5页

In vitro investigations of Staphylococcus aureus biofilms in physiological fluids suggest that current antibiotic delivery systems may be limited. 对生理液体中金黄色葡萄球菌生物膜的体外研究表明，目前的抗生素输送系统可能存在局限性。

IF 3.2 3区医学

European cells & materials Pub Date : 2022-02-02 DOI: 10.22203/eCM.v043a03

S Isguven, K Fitzgerald, L J Delaney, M Harwood, T P Schaer, N J Hickok

{"title":"In vitro investigations of Staphylococcus aureus biofilms in physiological fluids suggest that current antibiotic delivery systems may be limited.","authors":"S Isguven, K Fitzgerald, L J Delaney, M Harwood, T P Schaer, N J Hickok","doi":"10.22203/eCM.v043a03","DOIUrl":"10.22203/eCM.v043a03","url":null,"abstract":"Orthopaedic surgical site infections, especially when a hardware is involved, are associated with biofilm formation. Clinical strategies for biofilm eradication still fall short. The present study used a novel animal model of long-bone fixation with vancomycin- or gentamicin-controlled release and measured the levels of antibiotic achieved at the site of release and in the surrounding tissue. Then, using fluids that contain serum proteins (synovial fluid or diluted serum), the levels of vancomycin or gentamicin required to substantially reduce colonising bacteria were measured in a model representative of either prophylaxis or established biofilms. In the in vivo model, while the levels immediately adjacent to the antibiotic release system were up to 50× the minimal inhibitory concentration in the first 24 h, they rapidly dropped. At peripheral sites, values never reached these levels. In the in vitro experiments, Staphylococcus aureus biofilms formed in serum or in synovial fluid showed a 5-10 fold increase in antibiotic tolerance. Importantly, concentrations required were much higher than those achieved in the local delivery systems. Finally, the study determined that the staged addition of vancomycin and gentamicin was not more efficacious than simultaneous vancomycin and gentamicin administration when using planktonic bacteria. On the other hand, for biofilms, the staged addition seemed more efficacious than adding the antibiotics simultaneously. Overall, data showed that the antibiotics' concentrations near the implant in the animal model fall short of the concentrations required to eradicate biofilms formed in either synovial fluid or serum.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"43 ","pages":"6-21"},"PeriodicalIF":3.2,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9199942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial - Brothers in arms: regenerative biology and dentistry. 社论-战友:再生生物学和牙科。

IF 3.1 3区医学

European cells & materials Pub Date : 2022-01-03 DOI: 10.22203/eCM.v043a02

T A Mitsiadis, O Trubiani

引用次数: 1

Editorial - Disc Biology Special Issue 社论-光盘生物学特刊

IF 3.1 3区医学

European cells & materials Pub Date : 2022-01-03 DOI: 10.22203/eCM.v043a01

A Vernengo, Z Li, S Grad

引用次数: 1

Bioactive molecules for regenerative pulp capping. 再生髓盖生物活性分子。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-29 DOI: 10.22203/eCM.v042a26

L L Whitehouse, N H Thomson, T Do, G A Feichtinger

{"title":"Bioactive molecules for regenerative pulp capping.","authors":"L L Whitehouse, N H Thomson, T Do, G A Feichtinger","doi":"10.22203/eCM.v042a26","DOIUrl":"https://doi.org/10.22203/eCM.v042a26","url":null,"abstract":"Since the discovery of bioactive molecules sequestered in dentine, researchers have been exploring ways to harness their activities for dental regeneration. One specific area, discussed in this review, is that of dental-pulp capping. Dental-pulp caps are placed when the dental pulp is exposed due to decay or trauma in an attempt to enhance tertiary dentine deposition. Several materials are used for dental-pulp capping; however, natural biomimetic scaffolds may offer advantages over manufactured materials such as improved aesthetic, biocompatibility and success rate. The present review discusses and appraises the current evidence surrounding biomimetic dental-pulp capping, with a focus on bioactive molecules sequestered in dentine. Molecules covered most extensively in the literature include transforming growth factors (TGF-βs, specifically TGF-β1) and bone morphogenetic proteins (BMPs, specifically BMP-2 and BMP-7). Further studies would need to explore the synergistic use of multiple peptides together with the development of a tailored scaffold carrier. The roles of some of the molecules identified in dentine need to be explored before they can be considered as potential bioactive molecules in a biomimetic scaffold for dental-pulp capping. Future in vivo work needs to consider the inflammatory environment of the dental pulp in pulpal exposures and compare pulp-capping materials.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"415-437"},"PeriodicalIF":3.1,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39787802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Vertebral osteomyelitis is characterised by increased RANK/OPG and RANKL/OPG expression ratios in vertebral bodies and intervertebral discs. 椎体骨髓炎的特征是椎体和椎间盘中RANK/OPG和RANKL/OPG表达比升高。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-29 DOI: 10.22203/eCM.v042a27

S Lang, M Loibl, J Gläsner, M Simon, M Rupp, S Grad, C Neumann, V Alt, A Gessner, F Hanses

{"title":"Vertebral osteomyelitis is characterised by increased RANK/OPG and RANKL/OPG expression ratios in vertebral bodies and intervertebral discs.","authors":"S Lang, M Loibl, J Gläsner, M Simon, M Rupp, S Grad, C Neumann, V Alt, A Gessner, F Hanses","doi":"10.22203/eCM.v042a27","DOIUrl":"https://doi.org/10.22203/eCM.v042a27","url":null,"abstract":"Vertebral osteomyelitis (VO) is an infection of the spine mainly caused by bacterial pathogens. The pathogenesis leading to destruction of intervertebral discs (IVDs) and adjacent vertebral bodies (VBs) is poorly described. The present study aimed at investigating the connection between infection and bone/disc metabolism in VO patients. 14 patients with VO (infection group) and 14 patients with burst fractures of the spine (fracture group; control) were included prospectively. Tissue biopsies from affected IVDs and adjacent VBs were analysed by RT-qPCR for mRNA-expression levels of 18 target genes including chemokines, adipokines and genes involved in bone metabolism. Most importantly, the receptor activator of NF-κB/osteoprotegerin (RANK/OPG) expression ratio was drastically elevated in both VBs and IVDs of the infection group. In parallel, expression of genes of the prostaglandin-E2-dependent prostanoid system was induced. Such genes regulate tissue degradation processes via the triad OPG/RANK/RANKL as well as via the chemokines IL-8 and CCL-20, whose expression was also found to be increased upon infection. The gene expression of the adipokine leptin, which promotes inflammatory tissue degradation, was higher in IVD tissue of the infection group, whereas the transcription of omentin and resistin genes, whose functions are largely unknown in the context of infectious diseases, was lower in infected VBs. In summary, similar expression patterns of pro-inflammatory cytokines and pro-osteoclastogenic factors were identified in VBs and IVDs of patients suffering from VO. This suggests that common immuno-metabolic pathways are involved in the mechanisms leading to tissue degradation in VBs and IVDs during VO.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"438-451"},"PeriodicalIF":3.1,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39945894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells. TWIST1控制间充质干细胞的细胞衰老和能量代谢。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-25 DOI: 10.22203/eCM.v042a25

C Voskamp, L A Anderson, W J Koevoet, S Barnhoorn, P G Mastroberardino, G J van Osch, R Narcisi

{"title":"TWIST1 controls cellular senescence and energy metabolism in mesenchymal stem cells.","authors":"C Voskamp, L A Anderson, W J Koevoet, S Barnhoorn, P G Mastroberardino, G J van Osch, R Narcisi","doi":"10.22203/eCM.v042a25","DOIUrl":"https://doi.org/10.22203/eCM.v042a25","url":null,"abstract":"Mesenchymal stem cells (MSCs) are promising cells for regenerative medicine therapies because they can differentiate towards multiple cell lineages. However, the occurrence of cellular senescence and the acquiring of the senescence-associated secretory phenotype (SASP) limit their clinical use. Since the transcription factor TWIST1 influences expansion of MSCs, its role in regulating cellular senescence was investigated. The present study demonstrated that silencing of TWIST1 in MSCs increased the occurrence of senescence, characterised by a SASP profile different from irradiation-induced senescent MSCs. Knowing that senescence alters cellular metabolism, cellular bioenergetics was monitored by using the Seahorse XF apparatus. Both TWIST1-silencing-induced and irradiation-induced senescent MSCs had a higher oxygen consumption rate compared to control MSCs, while TWIST1-silencing-induced senescent MSCs had a low extracellular acidification rate compared to irradiation-induced senescent MSCs. Overall, data indicated how TWIST1 regulation influenced senescence in MSCs and that TWIST1 silencing-induced senescence was characterised by a specific SASP profile and metabolic state.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"401-414"},"PeriodicalIF":3.1,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39660723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dentine matrix metalloproteinases as potential mediators of dentine regeneration. 牙本质基质金属蛋白酶是牙本质再生的潜在介质。

IF 3.2 3区医学

European cells & materials Pub Date : 2021-11-24 DOI: 10.22203/eCM.v042a24

E Guirado, A George

引用次数: 0

Tendon mechanobiology in small-animal experiments during post-transection healing. 肌腱力学生物学在小动物实验中的应用。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-11-02 DOI: 10.22203/eCM.v042a23

T Notermans, H Hammerman, P Eliasson, H Isaksson

引用次数: 2

Current osteomyelitis mouse models, a systematic review. 目前骨髓炎小鼠模型，系统综述。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-21 DOI: 10.22203/eCM.v042a22

C Guarch-Pérez, M Riool, S Aj Zaat

{"title":"Current osteomyelitis mouse models, a systematic review.","authors":"C Guarch-Pérez, M Riool, S Aj Zaat","doi":"10.22203/eCM.v042a22","DOIUrl":"https://doi.org/10.22203/eCM.v042a22","url":null,"abstract":"Osteomyelitis is an inflammatory bone disease caused by an infecting microorganism leading to a gradual bone loss. Due to the difficulty in studying osteomyelitis directly in patients, animal models allow researchers to investigate the pathogenesis of the infection and the development of novel prophylactic, anti-inflammatory and antimicrobial treatment strategies. This review is specifically focused on the in vivo mouse osteomyelitis studies available in literature. Thus, a systematic search on Web of Science and PubMed was conducted using the query \"(infection) AND (mice OR mouse OR murine) AND (model OR models) AND (arthroplasty OR fracture OR (internal fixator) OR (internal fixation OR prosthesis OR implant OR osteomyelitis)\". After critical assessment of the studies according to the inclusion and exclusion criteria, 135 studies were included in the detailed analysis. Based on the model characteristics, the studies were classified into five subject groups: haematogenous osteomyelitis, post-traumatic osteomyelitis, bone-implant-related infection, peri-prosthetic joint infection, fracture-related infection. In addition, the characteristics of the mice used, such as inbred strain, age or gender, the characteristics of the pathogens used, the inoculation methods, the type of anaesthesia and analgesia used during surgery and the procedures for evaluating the pathogenicity of the infecting micro-organism were described. Overall, the mouse is an excellent first step in vivo model to study the pathogenesis, inflammation and healing process of osteomyelitis and to evaluate novel prophylaxis and treatment strategies.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"334-374"},"PeriodicalIF":3.1,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39538575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Bone infection: a clinical priority for clinicians, scientists and educators. 骨感染:临床医生，科学家和教育工作者的临床优先事项。

IF 3.1 3区医学

European cells & materials Pub Date : 2021-10-18 DOI: 10.22203/eCM.v042a21

T F Moriarty, G Muthukrishnan, J L Daiss, C Xie, K Nishitani, Y Morita, H Awad, K L de Mesy Bentley, E Masters, T Bui, M Yan, J Owen, B Mooney, S Gill, J Puetzler, J C Wenke, M Morgenstern, W-J Metsemakers, C Noll, A Joeris, R G Richards, E M Schwarz, S L Kates

{"title":"Bone infection: a clinical priority for clinicians, scientists and educators.","authors":"T F Moriarty, G Muthukrishnan, J L Daiss, C Xie, K Nishitani, Y Morita, H Awad, K L de Mesy Bentley, E Masters, T Bui, M Yan, J Owen, B Mooney, S Gill, J Puetzler, J C Wenke, M Morgenstern, W-J Metsemakers, C Noll, A Joeris, R G Richards, E M Schwarz, S L Kates","doi":"10.22203/eCM.v042a21","DOIUrl":"https://doi.org/10.22203/eCM.v042a21","url":null,"abstract":"Bone infection has received increasing attention in recent years as one of the main outstanding clinical problems in orthopaedic-trauma surgery that has not been successfully addressed. In fact, infection may develop across a spectrum of patient types regardless of the level of perioperative management, including antibiotic prophylaxis. Some of the main unknown factors that may be involved, and the main targets for future intervention, include more accurate and less invasive diagnostic options, more thorough and accurate debridement protocols, and more potent and targeted antimicrobials. The underlying biology dominates the clinical management of bone infections, with features such as biofilm formation, osteolysis and vascularisation being particularly influential. Based on the persistence of this problem, an improved understanding of the basic biology is deemed necessary to enable innovation in the field. Furthermore, from the clinical side, better evidence, documentation and outreach will be required to translate these innovations to the patient. This review presents the findings and progress of the AO Trauma Clinical Priority Program on the topic of bone infection.","PeriodicalId":11849,"journal":{"name":"European cells & materials","volume":"42 ","pages":"312-333"},"PeriodicalIF":3.1,"publicationDate":"2021-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39528870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3