Environmental and Experimental Botany最新文献

{"title":"Leaf chemical and structural properties govern foliar uptake of phosphorus from dust in chickpea","authors":"Elnatan Golan ,&nbsp;Avner Gross ,&nbsp;Nurit Agam ,&nbsp;Hagai Yasuor ,&nbsp;Ilana Shtein ,&nbsp;Ran Erel","doi":"10.1016/j.envexpbot.2025.106168","DOIUrl":"10.1016/j.envexpbot.2025.106168","url":null,"abstract":"<div><div>Certain plant species exhibit the capability to absorb phosphorus (P) directly from dust particles deposited on their leaves, particularly in P-limited environments. However, the underlying mechanisms that enable foliar uptake of P from insoluble sources such as dust minerals remain largely unexplored. This study investigates the chemical and structural properties of chickpea leaf surfaces that influence P absorption from dust deposition. We evaluated various chickpea varieties in a series of controlled experiments where P-rich dust was applied to their shoots. The relationship between P acquisition from dust and leaf surface pH, metabolite exudation and trichome density was examined. The tested varieties displayed variability in their leaf structural and chemical properties, which led to significantly different responses to foliar dust application. The most positive dust-responsive variety ('Mekomit') demonstrated a substantial 47–169 % increase in biomass in response to foliar dust application, whereas the most negatively responsive variety ('Cr205') exhibited a 28 % decrease in biomass following application. The positive response to dust was associated with lower leaf surface pH, higher trichome density, and enhanced metabolites exudation. Notably, the positively responsive variety showed increased exudation of sugars and organic acids, predominantly oxalic and malic acids from the leaf surfaces which facilitate P solubilization. These findings underscore the remarkable capacity of certain chickpea varieties to adapt their leaf characteristics in response to P deficiency, allowing them to absorb P from otherwise insoluble sources, such as airborne dust. This insight reveals a fundamental physiological mechanism that enhances our understanding of plant nutrient acquisition strategies under challenging environmental conditions.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"236 ","pages":"Article 106168"},"PeriodicalIF":4.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing citrus resilience to high temperature and intense light stress with Pseudomonas putida and Novosphingobium sp.","authors":"Fátima Terán ,&nbsp;Vicente Vives-Peris ,&nbsp;Aurelio Gómez-Cadenas ,&nbsp;Rosa M. Pérez-Clemente","doi":"10.1016/j.envexpbot.2025.106167","DOIUrl":"10.1016/j.envexpbot.2025.106167","url":null,"abstract":"<div><div>Adverse environmental conditions, aquifer contamination, and excessive fertilizer use have significantly impacted agricultural production, with these challenges worsening over the last few decades. Given the urgent need for more environmentally sustainable agricultural practices, recent studies have highlighted the potential of plant growth-promoting rhizobacteria (PGPR) as a biological alternative. PGPRs have been reported to function as biofertilizers and enhancers of plant tolerance to stress. Citrus, a globally important fruit crop, has been particularly affected by these environmental stressors. In this work, we aim to explore the effects of inoculating Cleopatra mandarin plants with PGPR strains <em>Pseudomonas putida</em> KT2440 and <em>Novosphingobium</em> sp. HR1a under combined conditions of heat stress and high light intensity (HSHL). Our findings demonstrate that inoculation with these strains provides significant protection against the detrimental effects of HSHL conditions. This protection is evident across multiple levels—phenotypically, physiologically, and molecularly—following the inoculation with <em>Pseudomonas putida</em> and <em>Novosphingobium</em> sp. Specifically, we observed under HSHL: (i) no leaf abscission in inoculated plants, (ii) improved photosynthesis efficiency under both non-stressed and stressed conditions, (iii) reduced malondialdehyde content, (iv) increased catalase activity, (v) upregulation of genes involved in stress tolerance (<em>WRKY40</em>, <em>WRKY76</em>, and <em>HSF30</em>), and (vi) increased sugar accumulation. Our study reveals that the use of <em>Pseudomonas putida</em> and <em>Novosphingobium</em> sp. offers a promising and environmentally sustainable approach for citriculture, particularly in mitigating the negative effects associated with climate change scenarios.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106167"},"PeriodicalIF":4.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of competitive constraints through long-term adaptation to high CO2 in mixed cultures of two diatom species","authors":"Yunyue Zhou ,&nbsp;Yuan Jia ,&nbsp;Peixuan Liu ,&nbsp;Baoyi Peng ,&nbsp;Jingyao Li ,&nbsp;Hao Zhang ,&nbsp;Leyao Xu ,&nbsp;Bin Huang ,&nbsp;Fangzhou Liu ,&nbsp;Jiamin Lin ,&nbsp;Fenghuang Wu ,&nbsp;Mengcheng Ye ,&nbsp;Jianrong Xia ,&nbsp;Peng Jin","doi":"10.1016/j.envexpbot.2025.106163","DOIUrl":"10.1016/j.envexpbot.2025.106163","url":null,"abstract":"<div><div>Diatoms play a pivotal role in marine ecosystems, contributing significantly to global primary production and carbon cycling. Understanding their responses to high CO₂ is critical for predicting oceanic changes under future climate scenarios. This study investigates the long-term adaptation of two diatom species, <em>Thalassiosira weissflogii</em> and <em>Phaeodactylum tricornutum</em>, to high CO₂ (1000 µatm) over 3.5–4 years and the consequences of their interactions in mixed cultures. Mono- and mixed-species cultures were maintained under both ambient (400 µatm) and high CO₂ conditions to assess various physiological performances. Our results revealed that most measured parameters (growth rate, photosynthesis and respiration rate, chlorophyll fluorescence parameters, and pigment concentration) were significantly reduced in mixed cultures compared to mono-cultures under both CO₂ conditions, underscoring the detrimental effects of interspecific competition. However, long-term adaptation to high CO₂ partially alleviated these reductions, particularly in photosynthesis, respiration, and chlorophyll-a content. These findings highlight the complex interplay between physiological adaptation and interspecific competition in shaping diatom responses to high CO₂. This study advances our understanding of the ecological and evolutionary implications of ocean acidification and underscores the importance of long-term experimental approaches for assessing the impacts of climate change on marine phytoplankton.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106163"},"PeriodicalIF":4.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single versus recurrent heat stress: A case study on the impact of a stress at flowering on the sorghum response to a subsequent post-flowering stress","authors":"Angelique Berger ,&nbsp;Belmys Cakpo ,&nbsp;Cyndi Suire ,&nbsp;Loic Pagan ,&nbsp;Sandrine Roques ,&nbsp;Raoul Vial ,&nbsp;Gregory Aguilar ,&nbsp;Nancy Terrier ,&nbsp;Christine Granier","doi":"10.1016/j.envexpbot.2025.106162","DOIUrl":"10.1016/j.envexpbot.2025.106162","url":null,"abstract":"<div><div>The increasing intensity and frequency of heat waves due to climate change pose a growing threat to global food security. Two experiments were conducted under controlled conditions using six temperature scenarios to compare the effects of single and recurrent heat stresses on maximum photosystem II quantum efficiency (<em>F</em>_v/<em>F</em>_m), above-ground plant biomass, grain yield and grain yield components in two sorghum genotypes. This study confirmed that the responses of grain number and thousand grain weight are highly dependent on stage development for both single and recurrent heat stresses. Recurrent heat stresses have synergistic effects, leading to around 20 % more yield losses than the cumulative responses to the corresponding single stresses. In contrast, <em>F</em>_v/<em>F</em>_m was less reduced by heat stress if the plants had been subjected to a previous stress. The large reduction in thousand grain weight under recurrent heat stresses may result in a strong remobilization of carbohydrates to sinks other than the main stem panicle. The increased tillering induced by single heat stresses and further amplified under recurrent heat stresses could partly explain this effect. The study highlights the complexity of plant responses to heat stress and the challenges of predicting outcomes under recurrent stress conditions.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106162"},"PeriodicalIF":4.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of soil water availability on apple tree physiology during heatwaves and on post-stress recovery","authors":"Nicola Giuliani ,&nbsp;Mario Wegher ,&nbsp;Dolores Asensio ,&nbsp;Damiano Zanotelli ,&nbsp;Carlo Andreotti ,&nbsp;Massimo Tagliavini","doi":"10.1016/j.envexpbot.2025.106161","DOIUrl":"10.1016/j.envexpbot.2025.106161","url":null,"abstract":"<div><div>In this study, the physiological response of potted apple trees to combined drought and heat stress was evaluated. After establishing different levels of soil water availability, the trees were exposed to a five-day simulated heatwave with daily maximum temperatures of 40°C. Stem water potential, leaf gas exchange, chlorophyll fluorescence, and tree transpiration were monitored before, during and after the combined application of heat and water stress, therefore providing insights into the extent and rapidity of the recovery. Drought caused stomatal closure that limited net photosynthesis and transpiration both at leaf and at tree level, leading to structural damage through leaf loss. On drought-stressed plants, chlorophyll fluorescence was significantly reduced by heat stress, suggesting additional leaf damage although net photosynthesis was not lower than under drought stress alone. On the other hand, well-watered trees showed low midday stem water potentials and high transpiration rates during the heatwave, while net photosynthesis was not affected. Water use efficiency of well-watered trees at 33°C was reduced to 60 % of that at 23°C. After the heatwave, transpiration rate in well-watered trees immediately declined to pre-stress levels, underscoring the strong atmospheric control on transpiration in apple trees. In drought-stressed trees, predawn stem water potential reached pre-stress values already on the first day of recovery. Stomatal conductance, net photosynthesis, and chlorophyll fluorescence, however, required a longer period to recover, indicating that drought stress induced transient hydraulic limitations. Nevertheless, all parameters fully recovered within five days after the end of the heatwave, showing that apple trees can withstand periods of combined heat and drought stress. The key role of water in modulating the response to heat stress highlights the need for improved irrigation management in apple orchards under climate change.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106161"},"PeriodicalIF":4.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic variations in sensitivity of root K+ and Ca2+ transporters to H2O2 explains differential salt tolerance in wheat and barley","authors":"Haiyang Wang ,&nbsp;Ping Yun ,&nbsp;Lana Shabala ,&nbsp;Zhong-Hua Chen ,&nbsp;Meixue Zhou ,&nbsp;Sergey Shabala","doi":"10.1016/j.envexpbot.2025.106160","DOIUrl":"10.1016/j.envexpbot.2025.106160","url":null,"abstract":"<div><div>Wheat and barley are known as important staple food worldwide, but their growth and yield are severely affected by soil salinity, prompting a need for regaining their stress tolerance lost during domestication, to meet food security targets under current climate scenarios. The bottle neck in this process is plant phenotyping. In the past decades, approach to plant phenotyping for salinity stress tolerance was predominantly driven by the need for a high throughput screening and focused on the whole-plant level traits by advocating various non-destructive and/or analytical methods. This approach, though useful for assessing overall plant performance under salinity stress, fails to account for tissue- and cell-specific operation of contributing mechanisms and, as a result, lack the predictive power. In this work, we propose and validate a new approach for phenotyping cereal crops for salinity stress tolerance by measuring H₂O₂-induced K⁺ and Ca²⁺ flux responses from mature root epidermis. By screening 44 barley, 20 durum and 20 bread wheat accessions, we show that tolerant genotypes reduce sensitivity of cation (Na⁺, K⁺ and Ca²⁺) permeable ion channels to ROS and argue that such desensitization may allow plants to efficiently regulate its ionic homeostasis in a cell- and tissue-specific manner, without compromising stress-induced ROS signaling to downstream targets, for transcriptional regulation purposes. Being conducted on young (4-d old) seedlings, this cell-based phenotyping platform offer breeders a possibility to target new (previously unexplored) traits and may be instrumental for assisting breeders in engineering salinity stress tolerance in future breeding programs.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"235 ","pages":"Article 106160"},"PeriodicalIF":4.5,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinated PIN7 and AUX1 responses to arsenite-restrained root growth in Arabidopsis","authors":"Lin Tao ,&nbsp;Xinyi Luo ,&nbsp;Junhuan Lv ,&nbsp;Zhishan Guo ,&nbsp;Yibo Yang ,&nbsp;Hui Wang ,&nbsp;Luping Gu ,&nbsp;Yang Yang ,&nbsp;Yanshan Lu ,&nbsp;Jiayi Wu ,&nbsp;Hu Zhu ,&nbsp;Ján Jásik ,&nbsp;Yalin Li ,&nbsp;Min Yu","doi":"10.1016/j.envexpbot.2025.106147","DOIUrl":"10.1016/j.envexpbot.2025.106147","url":null,"abstract":"<div><div>Arsenite (As^III), regarded as a hazard to human health and food safety, restrains root growth. This event has received little attention, and the mechanism underlying how As^III affects auxin dynamics to repress root growth remains unknown. Here, our results have suggested that As^III-inhibited root growth possibly involved an elevated auxin level in roots, as supported in multiple experiments such as (1) transgenic <em>DII-VENUS</em> and <em>DR5rev::GFP</em>; (2) IAA determination by enzyme-linked immunosorbent assay; (3) phenotype analysis of <em>taa1</em> (defective in auxin biosynthesis) mutant; and (4) the external application of 1-naphthylphthalamic acid (NPA) to manipulate auxin transport. This consequence could be explained through up-regulated transcriptional levels of auxin biosynthesis-related genes in whole plants. Phenotypes of auxin transport-related carriers have displayed that loss of PIN7 and AUX1, but not PIN1/2/3 transporters, ameliorated the extent of As^III-induced root growth inhibition. Moreover, As^III specifically enhanced the abundance of PM-localized PIN7 and AUX1 involved in transcriptional but not post-transcriptional regulation. A real-time <em>in vitro</em> observation of PM-localized PIN2 using the transgenic <em>pPIN2::PIN2-Dendra2</em> line has revealed that As^III did not influence the endocytosis of PM-localized PIN2 carriers in root apices. Overall, our results propose that As^III stress-elevated auxin level in the root apex possibly involves up-regulation transcriptional levels of auxin biosynthesis- and auxin transport-related genes but does not target PIN2 dynamics, finally, leading to auxin accumulation in root apices and root growth inhibition.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"234 ","pages":"Article 106147"},"PeriodicalIF":4.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of GA-induced seed dormancy release via MsGAI1-like-miR159a-MsMYB101 module in Magnolia sieboldii","authors":"Xin Xu ,&nbsp;Lin Liu ,&nbsp;Shixin Guan ,&nbsp;Xiaolin Zhang ,&nbsp;Mei Mei ,&nbsp;Xiujun Lu","doi":"10.1016/j.envexpbot.2025.106152","DOIUrl":"10.1016/j.envexpbot.2025.106152","url":null,"abstract":"<div><div><em>Magnolia sieboldii</em> K. Koch seeds belong to the morphophysiological dormancy type, and it is extremely difficult to germinate under natural conditions. Gibberellins (GAs) are crucial for facilitating seed dormancy release. However, the regulatory mechanisms by GA-mediated seed dormancy release in <em>M. sieboldii</em> remain unclear. Here, we reveal that exogenous GA₃ could quickly break the physiological dormancy of <em>M. sieboldii</em> seeds through morphological and physiological analyses. To investigate the role of miRNA159s in GA-induced seed dormancy release, <em>MsmiR159a</em> and its target gene <em>MsMYB101</em> were isolated and characterized. Spatial-temporal expression analyses showed that <em>MsmiR159a</em> and <em>MsMYB101</em> had opposite expression patterns. A cleavage interaction between <em>MsmiR159a</em> and <em>MsMYB101</em> was confirmed. Furthermore, we identified <em>GA-INSENSITIVE1-like</em> (<em>MsGAI1-like</em>), a negative regulator of GA signaling pathway, as directly binding to the promoter of miR159a to modulate the expression of MsmiR159a/<em>MsMYB101</em>. Yeast one-hybrid and electrophoretic mobility shift assays demonstrated that MsMYB101 directly binds to the promoter of the alpha-amylase gene <em>MsAMY2</em>. Dual-luciferase reporter assay indicated that MsMYB101 positively regulates <em>MsAMY2</em> expression, suggesting that MsmiR159a-<em>MsMYB101</em> module contributes to seed dormancy release by influencing starch metabolism. In conclusion, this study elucidates the potential mechanism underlying the response of the MsGAI1-like-miR159a-<em>MsMYB101</em> network to dormancy release in <em>M. sieboldii</em> seeds. These findings provide new insights into the molecular mechanisms of morphophysiological dormancy seeds.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"234 ","pages":"Article 106152"},"PeriodicalIF":4.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl jasmonate regulates panicle morphogenesis by mediating the negative effects of high temperature stress on carbon and nitrogen allocation and utilization","authors":"Weilu Wang ,&nbsp;Dongling Ji ,&nbsp;Xiaowu Yan ,&nbsp;Yu Wei ,&nbsp;Yunxia Han ,&nbsp;Weiyang Zhang ,&nbsp;Lijun Liu ,&nbsp;Hao Zhang ,&nbsp;Zhiqin Wang ,&nbsp;Zujian Zhang ,&nbsp;Jianchang Yang","doi":"10.1016/j.envexpbot.2025.106150","DOIUrl":"10.1016/j.envexpbot.2025.106150","url":null,"abstract":"<div><div>High-temperature stress (HTS) poses a serious threat to panicle development in rice. Plant hormones, including jasmonic acid (JA), play an important role in plant organ development. While the roles of IAA, cytokinin and gibberellin in heat stress have been studied, the research on JA in rice panicle morphogenesis under HTS during the early panicle differentiation period is still limited. In this study, we showed that HTS (day/night: 38℃ / 29℃) during the panicle differentiation period significantly reduced the number of differentiated spikelets and the number of grains per panicle. The correlation results suggest that this is related to antioxidant enzymes, carbon and nitrogen metabolism, and endogenous hormones, especially to carbon and nitrogen metabolism pathways. HTS limited the rate of carbon and nitrogen accumulation and redistribution in the plant, and the plant preferentially allocated more carbon and nitrogen to spikelets development under control conditions. The expression of JA synthesis and signaling genes was down-regulated under HTS, leading to a decrease in endogenous JA and MeJA content. Exogenous MeJA treatment optimized carbon and nitrogen metabolism by significantly enhancing the activities of nitrate reductase, glutamine synthetase, glutamate synthase, sucrose synthase, and sucrose phosphate synthase. Ultimately, the accumulation and allocation of carbon and nitrogen in panicle were improved, which greatly alleviated the high-temperature-induced decrease in differentiation spikelets and grain number. Overall, our results provide insight into the physiological effects of HTS during spikelets and panicle development; but, also suggest that HTS could be relieved with a supplemental application of MeJA.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"234 ","pages":"Article 106150"},"PeriodicalIF":4.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic changes in Arabidopsis thaliana exposed to extracellular self- and nonself-DNA: A reversible effect","authors":"Stefano Mazzoleni ,&nbsp;Laura Grauso ,&nbsp;Bruna de Falco ,&nbsp;Alfonso Mangoni ,&nbsp;Pasquale Termolino ,&nbsp;Emanuela Palomba ,&nbsp;Fabrizio Carteni ,&nbsp;Virginia Lanzotti","doi":"10.1016/j.envexpbot.2025.106149","DOIUrl":"10.1016/j.envexpbot.2025.106149","url":null,"abstract":"<div><div>Untargeted metabolomics analysis was used to assess at molecular level the plant reactions to extracellular DNA (exDNA) exposure. Thus, the effects on the metabolites profile of <em>A. thaliana</em> after exposure to self- and nonself-DNA have been investigated by NMR, LC-MS and chemometrics analyses. Results confirmed that self-DNA significantly induces the accumulation of different RNA constituents along with their cyclic analogues, in form of cyclic dimers, as well as methylated forms, increasing only in the self-DNA treatment. In addition, a deeper investigation of these samples showed the increase of several metabolites belonging to the classes of indoles, flavonoids, thiazoles and isothiocyanates. All these metabolites are known to be involved in plant growth and defence. Among these, isothiocyanates are known to affect the <em>A. thaliana</em> cell cycle with accumulation of cells in S-phase which is consistent with observations of self-DNA inhibition in other model organisms. Noteworthy, the early metabolomic changes induced by self-DNA were shown to be reversible when followed by a second exposure to extracellular nonself-DNA. These results highlight the relevance of the environmental balance between self- and nonself-DNA in the regulation of plant metabolism.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"234 ","pages":"Article 106149"},"PeriodicalIF":4.5,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}