Environmental and Experimental Botany最新文献

{"title":"Nitrogen form mediates sink strength and resource allocation of a C3 root crop under elevated CO2","authors":"","doi":"10.1016/j.envexpbot.2024.105892","DOIUrl":"10.1016/j.envexpbot.2024.105892","url":null,"abstract":"<div><p>Plant physiological processes alter with elevated carbon dioxide concentrations in the atmosphere (eCO₂), which affects the growth and yield potential of crops. Among plants with C₃ carbon fixation, root crops show highest yield response under eCO₂ which is suggested to be linked to their large carbon (C) sink strength. The high C gain under eCO₂ can be limited by processes that constrain sink capacity, such as nitrogen (N) supply. Different N sources may interact with eCO₂ and thus have variable impacts on carboxylation activity, N uptake efficiency and plant development. This study aims at contributing to a better understanding of sink-driven assimilation, re-allocation and finally growth processes under eCO₂ as a function of N-form. Radish (<em>Raphanus sativus</em> L. var. <em>sativus</em>), a C₃ tuber plant with strong sink strength, was used. The plants were grown in pots in climate chambers at 400 ppm (aCO₂) and 1000 ppm CO2 (eCO₂) with either pure nitrate or ammonium-dominated nutrition. A split plot design was applied. Plants were harvested after four weeks and physiological, morphological and chemical parameters in leaves and tubers were assessed. The N-form had no effect on N acquisition, but affected C and N partitioning into plant organs differently under eCO₂. N assimilation processes of nitrate-fed plants were focussed on leaves being both source and sink, and those of ammonium-fed plants on tubers, the strongly pronounced sink. Acclimation of CO₂ fixation due to eCO₂ did not occur for both N forms, probably due to altered sink strength and low N content in leaves. The N-form influences the sink-driven C and N balance and thus enables unrestricted carbon gain as well as the variation of organ development under future eCO₂ conditions. These processes can be utilized in cultivation and breeding.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0098847224002508/pdfft?md5=dd95ae2a436d3933cf30c191638b3d05&pid=1-s2.0-S0098847224002508-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental and genetic drivers of physiological and functional traits in a key canopy species","authors":"","doi":"10.1016/j.envexpbot.2024.105904","DOIUrl":"10.1016/j.envexpbot.2024.105904","url":null,"abstract":"<div><p>The resilience of forests worldwide is challenged by climate change. Large-scale tree mortality and dieback events have been documented across continents in recent decades. The adaptive capacity of forests is important for predicting forest resistance and resilience to future climates yet remains largely unknown. We grew 12 populations of a widespread foundation tree species (<em>Corymbia calophylla</em>), originating from different temperature and rainfall regimes, in two common garden trials in Western Australia that had similar temperature but contrasting rainfall conditions. We quantified intraspecific trait variation at these two sites to estimate genetically determined trait variation with climate origin (genetic adaptation) and trait variation associated with environment (phenotypic plasticity). We aimed to determine the 1) contribution of genetic and environmental factors on growth, functional, and physiological trait variation; 2) coordination of leaf traits within the context of the leaf economic spectrum (LES) in variable rainfall conditions; and 3) role of local or regional climate adaptation influencing tree growth and water use efficiency. Growth and physiological traits were differentially expressed across populations and sites, highlighting the importance of genetic adaptation and phenotypic plasticity. Leaf traits reflected a more water conservative strategy with higher water use efficiency, high foliar nitrogen content, and low specific leaf area, as predicted by the LES, in trees at the dry site measured in autumn after the warm summer. Local adaptation was detected in growth and leaf water use efficiency traits at the regional climate, not the local population, scale. Plants from the cool region had greater performance than those from the warm region in most plant traits. Home-site rainfall was not a good predictor of trait expression. The capacity of <em>C. calophylla</em> to respond to low water availability through genetic adaptation and phenotypic plasticity may enable it to maintain optimal performance in drier conditions associated with climate change.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0098847224002624/pdfft?md5=8e9ba873a10ab6a1f4ecf342fc109f51&pid=1-s2.0-S0098847224002624-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141704068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The UDP-glycosyltransferase gene OsUGT706E2 negatively regulates rice tolerance to blast disease and abiotic stresses","authors":"Pingli Chen,&nbsp;Liqun Jiang,&nbsp;Lanlan Zhang,&nbsp;Bingrui Sun,&nbsp;Shuwei Lv,&nbsp;Jing Zhang,&nbsp;Hang Yu,&nbsp;Xingxue Mao,&nbsp;Zhilan Fan,&nbsp;Chen Li,&nbsp;Wenfeng Chen,&nbsp;Qing Liu","doi":"10.1016/j.envexpbot.2024.105889","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105889","url":null,"abstract":"<div><p>Despite the crucial role of UDP-glycosyltransferases (UGTs) in various stress responses in plants, their biological functions in rice (<em>Oryza sativa</em> L.) remain poorly understood. In this study, we introduce a novel gene, <em>OsUGT706E2</em>, which is expressed at significantly higher levels in wild rice compared to cultivated rice. <em>OsUGT706E2</em> is active in multiple tissues and localizes to both the nucleus and cytoplasm. Its transcription is notably up-regulated in response to cold stress, blast disease, and several hormone treatments. Overexpression of <em>OsUGT706E2</em> markedly reduces seedling tolerance to blast disease, cold, and osmotic stress, whereas knocking out <em>OsUGT706E2</em> enhances seedling tolerance to blast disease and osmotic stress. The expression levels of stress-related genes in <em>OsUGT706E2</em> overexpressing plants were significantly lower compared to wild-type plants following stress treatment. Conversely, <em>OsUGT706E2</em> knockout plants exhibited markedly higher expression levels of these genes than the control plants after stress treatment. Metabolome analysis further indicated that <em>OsUGT760E2</em> influences metabolite content in rice. Specifically, <em>OsUGT760E2-</em>overexpressing seedlings had higher amino acid content and lower lipid content compared to wild-type seedlings, while <em>OsUGT760E2-</em>knockout seedlings showed lower amino acid content and higher lipid content than control seedlings. These findings suggest that <em>OsUGT760E2</em> negatively regulates resistance to blast disease as well as tolerance to cold and osmotic stress in rice. As a result, <em>OsUGT760E2</em> represents a promising target for enhancing rice stress tolerance through gene editing approaches.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0098847224002478/pdfft?md5=c6930fe5e8c48873d7d926ca6f841fc8&pid=1-s2.0-S0098847224002478-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The bZIP gene family in the halophyte Limonium bicolor: Identification, expression analysis, and regulation of salt stress tolerance","authors":"Fanxia Meng ,&nbsp;Zhihui Zhu ,&nbsp;Juying Wang ,&nbsp;Xiaofang Chen ,&nbsp;Kai Ning ,&nbsp;Hualing Xu ,&nbsp;Min Chen","doi":"10.1016/j.envexpbot.2024.105896","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105896","url":null,"abstract":"<div><p>The basic region/leucine zipper transcription factors (bZIPs) are one of the largest transcription factor families in eukaryotes, and play a key role in growth, development, and response to abiotic stresses. <em>Limonium bicolor</em> is a typical recretohalophyte, which can excrete excess salts from body to outside through the salt glands located on the stems and leaves to maintain ionic balance in the body. In this study, 58 <em>L. bicolor</em> bZIP members (<em>LbbZIP1</em>–<em>58</em>) were identified, which were unevenly distributed on 8 chromosomes and divided into 11 groups. The bioinformatics analysis revealed strong colinearity between <em>LbbZIP</em>s and <em>GmbZIP</em>s of soybean (<em>Glycine max</em>). And promoter <em>cis</em>-acting elements of <em>LbbZIPs</em> were related to stress responses and phytohormone responses. Most <em>LbbZIP</em> members responded to abiotic stresses such as NaCl, PEG, and ABA. Silencing <em>LbbZIP28</em> in <em>L. bicolor</em> increased salt glands density and salt secretion capacity of leaves, and salt tolerance by means of up-regulating the expression levels of genes involved in salt gland development and ion transport. These results provide new information for <em>LbbZIPs</em> and help to interpret salt gland development and salt secretion in <em>L. bicolor</em>. Our results will provide a reference for a deeper understanding of the molecular foundation of <em>LbbZIPs</em> in <em>L.bicolor</em>.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential response of roots and leaves to combined heat and salinity stresses in tomato plants","authors":"Tania Mesa ,&nbsp;Alejandra Romero ,&nbsp;Sergi Munné-Bosch","doi":"10.1016/j.envexpbot.2024.105890","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105890","url":null,"abstract":"<div><p>Despite our understanding of plant responses to single stresses, knowledge on how plants respond to combined abiotic factors and the underlying hormonal regulation is still very limited. Here, we aimed to examine the plant response to combined heat and salt stresses in tomato plants, the underlying hormonal response and the effectiveness of methyl jasmonate application in its alleviation. We measured fruit production and various stress markers in both roots and leaves, together with endogenous contents of stress-related phytohormones (including abscisic acid, salicylic acid and jasmonates) in tomato plants (<em>Solanum lycopersicum</em> cv. Micro-Tom), exposed to combined stress. In addition, we evaluated the effectiveness of a methyl jasmonate treatment as a priming agent to alleviate the negative effects of stress, with an emphasis on evaluating the effects of this hormone on triggering antioxidant protection by enhancement of vitamin E contents. Plants responded differently to combined stress treatment than to single stresses, but this differential response was organ-specific, with roots being more sensitive to stress than leaves. Both abscisic acid and jasmonates were involved in the plant response to combined stress but leaves and roots responded differently. Furthermore, abscisic acid and jasmonates correlated with vitamin E accumulation, most particularly in roots. Foliar application of methyl jasmonate at the flowering stage in plants challenged with combined stress did not improve fruit production but resulted in enhanced vitamin E accumulation in leaves. It is concluded that (i) roots and leaves show a differential sensitivity to both single and combined heat and salt stresses, (ii) the response of abscisic acid and jasmonates in plant stress responses seems to be markedly organ dependent, and (iii) foliar methyl jasmonate increased vitamin E accumulation under combined stress in tomato plants.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S009884722400248X/pdfft?md5=90bb842d2cf1b351ddea5a6237fd9e9f&pid=1-s2.0-S009884722400248X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chilling at grain filling stage reduced rice grain protein content: An experimental and modeling study","authors":"","doi":"10.1016/j.envexpbot.2024.105891","DOIUrl":"10.1016/j.envexpbot.2024.105891","url":null,"abstract":"<div><p>Climate change has increased the trend in the intensity of global extreme weather events, including chilling. Nitrogen is one of the most essential nutrients for rice growth and development. Chilling will limit the uptake and translocation of nitrogen and the formation of grain protein in rice plants. The experiment conducted in the climate chamber with chilling stress applied at the grain filling stage revealed different effects on protein concentration in aboveground organs and caused asymmetry in grain protein contents (GPC) and grain protein yield (GPY). Chilling stress applied during the grain filling stage reduced the accumulation rate of rice grain protein by inhibiting the nitrogen uptake and the translocation from vegetative organs to grains, which resulted in a 35 % decrease in grain nitrogen accumulation and a 92 % increase in vegetative organs during grain filling. Consequently, the average nitrogen harvest index is reduced by 12 %. Nitrogen accumulation was severely affected when cooling degree days (CDD) ≥ 70 °C days. As the chilling intensity increased, the decrease of GPY was more significant than that of GPC. Moreover, we improved the grain temperature-nitrogen uptake function under chilling stress based on the relationship between CDD and the reduction in rice grains. By comparing the improved function and the modules in the existing crop model using the datasets from open field and artificial control experiments, we demonstrated that the current research on quantifying rice nitrogen uptake at extreme temperature stress needed further improvement. The effects of environmental stress on grain nitrogen accumulation are complex. Future studies should pay more attention to the ability of extreme temperature stress to affect nitrogen accumulation in various rice organs.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141694206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Individual grain mass of inbred rice cultivars does not benefit from elevated [CO2]","authors":"Yao Huang ,&nbsp;Wenjuan Sun ,&nbsp;Zhenghua Hu","doi":"10.1016/j.envexpbot.2024.105888","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105888","url":null,"abstract":"<div><p>Climate warming has led to a reduction of global crop yields. Elevated atmospheric [CO₂] is believed to promote crop production by increasing photosynthesis, and thus partly offset the yield losses due to climate warming. However, photosynthetic acclimation may occur when the plant is exposed to long-term high [CO₂] conditions, suggesting that elevated [CO₂] (e[CO₂]) might not bring benefits for cereal crops as the growing season proceeds. To assess the effect of long-term e[CO₂] on rice yield, particularly on individual grain mass determined post-heading, we synthesized existing data from FACE (free-air CO₂ enrichment) experiments across four locations in Japan and China. We also conducted a five-year field experiment with different [CO₂] treatments using OTC (open-top chamber) facility. A novel experiment of pot replacement at heading was conducted in 2018 to evaluate the effect of post-heading e[CO₂] on individual grain mass of rice. Meanwhile, we measured net photosynthetic rates under ambient [CO₂] (a[CO₂]) and e[CO₂] (ambient + 200 μmol mol⁻¹) at different developmental stages to identify the occurrence of photosynthetic acclimation. We show that FACE condition did not increase individual grain mass across thirty-six inbred rice cultivars at various rates of nitrogen application, and that the replacement of potted plants either from a[CO₂] to e[CO₂] or from e[CO₂] to a[CO₂] did not impact the individual grain mass. The yield benefit from e[CO₂] was primarily attributed to an increase in the spikelet density determined pre-heading. We conclude that the individual grain mass of inbred rice does not benefit from e[CO₂], which is most likely attributed to a loss of the advantage in CO₂ gain induced by photosynthetic acclimation. Our findings suggest the necessity for selective breeding strategies to make better use of post-heading e[CO₂] and for crop modelers to incorporate updated knowledge into models in the context of elevated [CO₂].</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted roles of Arabidopsis heat shock factor binding protein in plant growth, development, and heat shock response","authors":"Ya-Chen Huang ,&nbsp;Chin-Cheng Liu ,&nbsp;Yi-Jie Li ,&nbsp;Chi-Min Liao ,&nbsp;Sandeep Vivek ,&nbsp;Guan-Lin Chuo ,&nbsp;Chih-Yen Tseng ,&nbsp;Zhi-Qing Wu ,&nbsp;Tomoo Shimada ,&nbsp;Noriyuki Suetsugu ,&nbsp;Masamitsu Wada ,&nbsp;Chin-Mei Lee ,&nbsp;Tsung-Luo Jinn","doi":"10.1016/j.envexpbot.2024.105878","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105878","url":null,"abstract":"<div><p>Heat shock factor-binding protein (HSBP) is a 10 kDa protein in plants and animals and consists exclusively of a coiled-coil domain. During the recovery phase following a heat shock response, HSBP relocates from the cytoplasm to the nucleus in Arabidopsis (<em>Arabidopsis thaliana</em>), where it interacts with heat shock factors (HSFs). Here, we found that 16 out of the 19 functional known HSFs can interact with HSBP. Besides, our results indicate that HSBP negatively regulates <em>HSF</em> gene expression during normal growth conditions and recovery from heat shock. Expanding our understanding of HSBP's physiological functions during regular growth, co-immunoprecipitation and mass spectrometry analysis identified 16 coiled-coil domain-containing proteins co-immunoprecipitated with HSBP. These proteins encompass HSP70s, all components of the MAIGO2 complex, COP1-interactive protein1 (CIP1), CIP1-like protein, kinesin-like protein for actin-based chloroplast movement1 and 2 (KAC1/2), and HSBP itself. By examining mutant plants lacking HSBP and its interacting proteins, we elucidated their functional relationships. Our findings underscore the indispensability of the HSBP coiled-coil heptad repeat for interacting with its partners and its crucial role in growth, development, and the heat shock response. In addition to its involvement in heat shock response, Arabidopsis HSBP, a discreet small regulatory protein, exerts multiple regulatory functions in hypocotyl elongation, flowering time, chloroplast photo-relocation, and seed development.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141595647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo imaging of nickel-rich laticifers: A breakthrough in metal hyperaccumulation","authors":"","doi":"10.1016/j.envexpbot.2024.105877","DOIUrl":"10.1016/j.envexpbot.2024.105877","url":null,"abstract":"<div><p>The discovery of the nickel-rich latex of the New Caledonian endemic tree <em>Pycnandra acuminata</em> introduced the term ‘hyperaccumulator’ and gave rise to a new field of research. This then instigated a global quest for these unusual hyperaccumulator plants, even while the underlying mechanisms of nickel acquisition, transport, and internal elemental distribution remained unknown for this original laticifer-bearing hyperaccumulator plant. Here we reveal for the first time the distribution of nickel-filled laticifers in the different plant organs of <em>P. acuminata</em>. The pressurised nickel laticifers were imaged multimodally with a combination of synchrotron X-ray fluorescence (XRF) microscopy, microtomography (XRF-μCT) and synchrotron X-ray phase contrast imaging microtomography (PCI-μCT). These advanced synchrotron methodologies allowed for complimentary non-invasive reconstructions of an <em>in-situ</em> model of the laticiferous system in this species. The data shows the distribution of the nickel-rich laticifers within whole plant tissues from roots to apical tip, thus suggesting nickel trafficking in the laticifer network. The extraordinary concentration of nickel within <em>P. acuminata</em> laticifers functions as an effective natural tracer for XRF-μCT and PCI-μCT to probe the structure and organization of these cells, thereby permitting insights into the development and physiological functioning of this unique duct system.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0098847224002351/pdfft?md5=8fd45f76d4146301462c536b1e397ff7&pid=1-s2.0-S0098847224002351-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics analysis of bahia grass (Paspalum notatum) inoculated with arbuscular mycorrhizal fungi exposed to soil Cd stress","authors":"Zhengjun Feng ,&nbsp;Ning Liu ,&nbsp;Panpan Tu ,&nbsp;Yan Zou ,&nbsp;Miroslav Vosatka ,&nbsp;Zhonghe Zhao ,&nbsp;Jie Chen ,&nbsp;Huiping Song","doi":"10.1016/j.envexpbot.2024.105867","DOIUrl":"https://doi.org/10.1016/j.envexpbot.2024.105867","url":null,"abstract":"<div><p>Heavy metals can adversely affect the growth and metabolic processes of plant. Arbuscular mycorrhizal (AM) fungi, which colonize the roots of most plants, may change the uptake and resistance to heavy metals by altering plant metabolism. But few published papers focused on how AM fungi enhanced plant tolerance to heavy metals at the metabolomics level. Therefore, our study incorporated LC-MS technology to explore the molecular mechanism by which AM fungi improved cadmium (Cd) tolerance of Bahia grass (<em>Paspalum notatum</em>) at the metabolomic scale. The results showed that AM fungi increased growth of Bahia grass, and enhanced its uptake and tolerance of Cd. Infection with AM fungi significantly raised the levels of both primary metabolites (amino acid, carbohydrate, organic acid, etc.) and secondary metabolites (such as terpenoids, phenolic compounds, and alkaloids), which in turn improved the photosynthesis efficiency, osmoregulation, and antioxidation defense of the plant, thereby enhancing the Cd tolerance of Bahia grass. Furthermore, under high concentration of Cd, both TCA cycle and lipid metabolism were generally suppressed, and AM fungi inoculation alleviated the effects and normalized the TCA cycle and lipid metabolism. In all, the metabolic alterations identified here provided insights into the mechanisms by which AM fungi enhanced plant tolerance to heavy metals.</p></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}