Environmental and Experimental Botany最新文献

{"title":"Molecular and metabolomics analysis reveals new insight into the mechanism underlying Iris halophila Pall. IhCHS1-mediated regulation of plant salt tolerance","authors":"Qingquan Liu ,&nbsp;Xi Gu ,&nbsp;Yongxia Zhang ,&nbsp;Ting Zhang ,&nbsp;Yinjie Wang ,&nbsp;Om Parkash Dhankher ,&nbsp;Shijie Tang ,&nbsp;Haiyan Yuan","doi":"10.1016/j.envexpbot.2024.106080","DOIUrl":"10.1016/j.envexpbot.2024.106080","url":null,"abstract":"<div><div>Soil salinity represents a significant threat to agricultural productivity. The identification of salt response genes from halophytes is of great significance for improving the resistance of glycophytic crops to salt stress. <em>Iris halophila</em> Pall. is an important ornamental and medicinal halophyte that exhibits strong resistance to salt stress and is rich in flavonoids. Previously, transcriptome analysis revealed that chalcone synthase (CHS)-catalyzed flavonoid biosynthesis is involved in the response of <em>I. halophila</em> to high salt stress. However, the regulatory mechanism of CHS on plant metabolome under salt stress remains unclear. In this study, the function of <em>IhCHS1</em> gene in regulating plant salt tolerance and metabolism was investigated. The results demonstrated that <em>IhCHS1</em> was upregulated in <em>I. halophila</em> under salt stress. Transgenic <em>Arabidopsis</em> overexpressing <em>IhCHS1</em> exhibited enhanced salt tolerance at both the seedling and mature stages. Physiological and metabolomic analysis indicated that the overexpression of <em>IhCHS1</em> resulted in a reduction in the degree of membrane lipid peroxidation and an increase in proline content, antioxidant enzyme activities, and the abundance of several flavonoids and other phenylpropanoid compounds<em>.</em> Interestingly, <em>IhCHS1</em> overexpression also significantly stimulated the accumulation of JA and IAA biosynthesis and signal transduction-related compounds, and altered the profiles of oligopeptides and nucleotide metabolites under salt stress. Our findings will provide new insight into the molecular mechanism of <em>IhCHS1</em>-mediated salt tolerance in plants and contribute to the development of strategies for cultivation of crop species with high salt tolerance and high metabolite accumulation on saline-alkali soil.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106080"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary responses of dominant and companion species along the precipitation gradient in a typical steppe from 1985 to 2022","authors":"Yi Zhou ,&nbsp;Shenghua Chang ,&nbsp;Xiaojuan Huang ,&nbsp;Wenjun Wang ,&nbsp;Fujiang Hou ,&nbsp;Yanrong Wang ,&nbsp;Zhibiao Nan","doi":"10.1016/j.envexpbot.2024.106075","DOIUrl":"10.1016/j.envexpbot.2024.106075","url":null,"abstract":"<div><div>Population dynamics may fluctuate dramatically over time in response to changing climate conditions. Thus, assessing how trade-offs in growth and reproduction of plant population respond to climate change may be key for predicting and conserving complex communities, especially in the context of different precipitation. Here, using a long-term experiment along the precipitation gradient, we investigated trade-offs in growth and reproduction of dominant species, <em>Artemisia capillaris</em> and companion species, <em>Aster altaicus</em>, as well as their climate-driven mechanisms. The impact of <em>A. capillaris</em> and <em>A. altaicus</em> on community above-ground biomass (AGB) and species richness (SR) were also evaluated. Our findings indicate that, over the past 37 years, <em>A. capillaris</em> exhibited lateral growth and asexual reproduction across the three sites. In contrast, <em>A. altaicus</em> tended to grow vertically and reproduce sexually, with a significant increase in its AGB. Moreover, the influence of climate fluctuation, characterized by variations in temperature and precipitation over specific time scales, on the two populations diminished from north to south. Long-term average climate, reflecting mean temperature and precipitation, and current climate conditions during the sampling year primarily influenced changes in <em>A. capillaris</em> and <em>A. altaicus</em> in south, respectively. Additionally, from north to south, the contribution of <em>A. altaicus</em> to community AGB and SR increased, while the contribution of <em>A. capillaris</em> to community AGB and SR decreased. Community AGB increased as the coupling index between <em>A. capillaris</em> and <em>A. altaicus</em> intensified. These findings can help us to better understand the coupling of plant traits, populations and communities, aid in the protection of grasslands under future climate change scenarios.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106075"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay among hormones, antioxidants, and redox signaling in abiotic stress responses","authors":"Camila Luiza Delaix ,&nbsp;Andressa Tomiozzo ,&nbsp;Guilherme Weber ,&nbsp;Yugo Lima-Melo ,&nbsp;Alexandre Nascimento de Vargas ,&nbsp;Márcia Margis-Pinheiro ,&nbsp;Thomaz Stumpf Trenz","doi":"10.1016/j.envexpbot.2024.106081","DOIUrl":"10.1016/j.envexpbot.2024.106081","url":null,"abstract":"<div><div>Abiotic stresses are major limiting factors in plant development, delaying growth and reducing crop yield. These stressors induce the formation of reactive oxygen species (ROS), which can be harmful at high concentrations but also act as signaling molecules. Plants display robust antioxidative machinery to scavenge excess ROS and maintain nontoxic ROS levels. Plant hormones, specialized signaling molecules, are not only essential in plant growth and development, but also transducing stress signals, profoundly affecting the antioxidative machinery and triggering plant adaptive responses. In this context, respiratory burst oxidase homologous proteins are essential hubs that mediate phytohormone-induced ROS production, linking phytohormonal signals to important processes, such as stomatal closure. This review highlights the significant role of abscisic acid, jasmonic acid, salicylic acid, and brassinosteroids in enhancing antioxidative responses under abiotic stress conditions. Additionally, we emphasize the importance of redox post-translational modifications involved in hormone signaling.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106081"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating transcriptomics and metabolomics to uncover key metabolic mechanisms of mistletoe parasitism on diverse hosts","authors":"Zhaoxiang Zeng ,&nbsp;Xiaoliu Hu ,&nbsp;Xueyan Zhao ,&nbsp;Xingliang Xiang ,&nbsp;Lijun Zhang ,&nbsp;Shan Cao ,&nbsp;Yurong Zhang ,&nbsp;Cheng Chen ,&nbsp;Rongzeng Huang ,&nbsp;Shuna Jin ,&nbsp;Chengwu Song","doi":"10.1016/j.envexpbot.2024.106077","DOIUrl":"10.1016/j.envexpbot.2024.106077","url":null,"abstract":"<div><div>Mistletoe (<em>Viscum coloratum</em> (Komar.) Nakai) is a parasitic plant with medicinal and culinary uses, yet its parasitic mechanisms on host species are largely unexplored. In this study, we performed an integrated transcriptomic and metabolomic analysis of mistletoe branches and leaves parasitizing on <em>Tilia amurensis</em> Rupr., <em>Ulmus pumila</em> L., and <em>Populus ussuriensis</em> Kom., investigating the potential molecular and metabolic mechanisms of mistletoe influenced by these hosts. Our results revealed notable differences in the transcriptional information of mistletoe branches and leaves across various host species, which was also reflected in their metabolic profiles. At the transcriptional level, lipid metabolism, carbohydrate metabolism and amino acid metabolism were the main pathway influenced by various hosts. Flavonoids and lipids were the primary differential components in the metabolic profile of mistletoe. Combined metabolomic and transcriptomic analyses demonstrated that glycerophospholipid metabolism comprising lipids, was a vital pathway for the activities of mistletoe parasites on their hosts. In this pathway, most metabolites, such as LysoPC, and the majority of genes, such as LYPLA2 and DPP1, exhibited the highest expression levels in <em>Tilia amurensis</em> Rupr. Mistletoe parasitizing <em>Tilia amurensis</em> Rupr., whether on branches or leaves, exhibited very significant differences compared to those parasitizing <em>Ulmus pumila</em> L. and <em>Populus ussuriensis</em> Kom. The findings provide key insights into the molecular mechanisms of mistletoe’s parasitic activities and how different hosts affect its transcriptional and metabolic profiles.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106077"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating ABA-dependent growth and development by overexpressing cytochrome P450 ABA 8′-hydroxylase in Medicago truncatula","authors":"Shuwen Li ,&nbsp;Jianbo Yuan ,&nbsp;Fufei Zhou ,&nbsp;Yaling Liu ,&nbsp;Hong Xie ,&nbsp;Wei Jia ,&nbsp;Yuehui Chao ,&nbsp;Liebao Han","doi":"10.1016/j.envexpbot.2024.106060","DOIUrl":"10.1016/j.envexpbot.2024.106060","url":null,"abstract":"<div><div>Abscisic acid (ABA) is a pivotal phytohormone involved in regulating various aspects of plant growth, development, and responses to environmental stress. The Cytochrome P450 family member ABA 8′-hydroxylase (8′OH-ABA) is proposed to play a central role in the catabolic degradation of ABA. In the present study, the <em>8'OH-ABA</em> gene from <em>Medicago truncatula</em> was isolated and functionally characterized using transgenic overexpression approaches. Under non-stress conditions, plants overexpressing <em>8'-OH-ABA</em> displayed notable phenotypic variations compared to wild-type plants, including altered leaf morphology, an extended lifespan, and delayed flowering. Scanning electron microscopy (SEM) analysis revealed a reduction in cell spacing and curvature at the leaf margins, which was attributed to the smaller size of epidermal cells in the stem, ultimately contributing to a slower growth rate. Furthermore, these overexpressing plants exhibited heightened sensitivity to drought stress, an effect closely associated with 8′OH-ABA expression. Transcriptome analysis revealed 3814 differentially expressed genes (DEGs), with 13 genes enriched in the \"abscisic acid-activated signaling pathway\" and 29 in the \"carotenoid biosynthesis\" pathway. Notably, we identified genes directly linked to ABA responses, including the <em>ABA 8′-hydroxylase CYP707A2</em>, the transcription factor gene <em>MYC2</em>, and the <em>cytochrome P450 enzyme CYP78A5</em>, which regulates organ size and leaf development. Collectively, these findings indicated the regulatory role of 8’OH-ABA in plant development and drought stress response, thereby highlighting the importance of ABA signaling in these biological processes.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106060"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The knockout of tobacco NtCBL10 inhibits leaf Cl- accumulation and leads to light-dependent necrosis & light-independent chlorosis under salt stress","authors":"Jingjing Mao ,&nbsp;Guang Yuan ,&nbsp;Richard G.F. Visser ,&nbsp;Yuling Bai ,&nbsp;Gang Xu ,&nbsp;Lin Xue ,&nbsp;Dongping Mao ,&nbsp;Haobao Liu ,&nbsp;Yang Ning ,&nbsp;Qian Wang ,&nbsp;C. Gerard van der Linden","doi":"10.1016/j.envexpbot.2024.106083","DOIUrl":"10.1016/j.envexpbot.2024.106083","url":null,"abstract":"<div><div><em>CBL10</em> was shown to be a key gene for salt tolerance in <em>Arabidopsis thaliana</em>. In this study, we evaluated the role of <em>CBL10</em> in the tobacco salt tolerance response by characterizing the gene editing-induced loss-of-function knockout mutants of the two <em>NtCBL10</em> homeologous genes <em>NtCBL10A</em> and <em>NtCBL10B</em>. The importance of <em>NtCBL10</em> for the response to salinity was evidenced by the salt supersensitivity of the <em>Nt</em>-<em>cbl10a10b</em> double mutants, with fast-developing chlorosis and severe necrotic lesions on leaves. Stomatal conductance and photochemical efficiency of photosystem 2 (PhiPS2) of the <em>Nt</em>-<em>cbl10a10b</em> double mutant were significantly inhibited already at a very early stage of the salt stress response. Leaf Na⁺ concentrations were not much affected in these plants, but the Cl^- content of the <em>Nt</em>-<em>cbl10a10b</em> double mutants was significantly lower than that of wild-type plants, which is the first report of <em>CBL10</em> in the regulation of Cl^- homeostasis. Interestingly, the necrosis phenotype of <em>Nt</em>-<em>cbl10a10b</em> double mutants was dependent on light, while the chlorosis phenotype of <em>Nt</em>-<em>cbl10a10b</em> double mutants was light-independent. Different from the previous studies that focus on the role of <em>CBL10</em> in Na⁺ homeostasis regulation, this study indicates that <em>NtCBL10</em> is a key component in regulating multiple aspects of ion homeostasis under salt stress.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106083"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alfalfa MsGAD2 induces γ-aminobutyric acid accumulation and enhances Cd resistance in transgenic tobacco","authors":"Bo Qin ,&nbsp;Minglong Sun ,&nbsp;Hongzhen Liu ,&nbsp;Kexin Wang ,&nbsp;Hongrui Zhang ,&nbsp;Tie Li ,&nbsp;Dongmei Li ,&nbsp;Huihui Zhang","doi":"10.1016/j.envexpbot.2024.106058","DOIUrl":"10.1016/j.envexpbot.2024.106058","url":null,"abstract":"<div><div>The present study compared two alfalfa varieties with different Cd-stress resistance levels: <em>Medicago sativa</em> ‘Zhaodong’ (ZD, resistant) and ‘Zhongmu No.1’ (ZM, sensitive). While the varieties showed no significant difference in Cd content under Cd stress, ZD exhibited greater adaptability, indicated by lower suppression of PSII activity, photosynthetic carbon assimilation, and reduced ROS damage. Weighted gene co-expression network analysis (WGCNA) based on RNA-seq data revealed that differentially expressed genes (DEGs) associated with physiological resistance indicators were significantly enriched in KEGG pathways related to proline (PRO) and γ-aminobutyric acid (GABA) synthesis, including alanine, aspartate and glutamate metabolism (map00250), arginine biosynthesis (map00220), and arginine and proline metabolism (map00330). Notably, the accumulation of proline and GABA was higher in the resistant alfalfa variety ZD. Furthermore, a key gene in the GABA synthesis pathway, <em>MsGAD2</em>, was identified as potentially playing a crucial role in the Cd-stress adaptation. To investigate its role in Cd stress resistance, alfalfa <em>MsGAD2</em> was heterologously overexpressed in tobacco. <em>MsGAD2</em> overexpression significantly enhanced GABA synthesis in tobacco leaves under both control and, especially, Cd stress conditions. <em>MsGAD2</em> overexpression also effectively prevented the decline of chlorophyll in tobacco leaves under Cd stress. It achieved this by stabilizing the activities of PSII and PSI and optimizing energy distribution within the PSII reaction center, thereby alleviating photosynthetic inhibition. The accumulation of GABA further enhanced the activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in tobacco leaves under Cd stress, thus promoting PRO accumulation and mitigating oxidative damage associated with Cd exposure. Thus, PRO and GABA accumulation likely contributed to ZD’s superior adaptability to Cd stress compared to ZM. Thus, <em>MsGAD2</em> played a crucial role in enhancing Cd tolerance in transgenic tobacco. These findings not only elucidate the mechanisms by which GABA is involved in Cd-stress adaptation, but also provide valuable genetic resources for breeding Cd-resistant alfalfa.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106058"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms activating trace heavy metals in the rhizosphere microenvironment of Amaranthus hypochondriacus L.","authors":"Xia Zhong ,&nbsp;Yuexin Fu ,&nbsp;Guo Yu ,&nbsp;Pingping Jiang ,&nbsp;Shaohong You ,&nbsp;Jie Liu","doi":"10.1016/j.envexpbot.2024.106067","DOIUrl":"10.1016/j.envexpbot.2024.106067","url":null,"abstract":"<div><div>Soil heavy metal activation is closely related to rhizosphere soil pH, O₂ and enzyme activity levels. In this study, we used laser ablation inductively coupled plasma<img>mass spectrometry (ICP<img>MS), planar optode (PO), soil in situ enzyme spectrum analysis, and the diffusive gradients in thin films (DGT) method to investigate heavy metal accumulation in <em>Amaranthus hypochondriacus</em> L. growing in polluted soil (Cd, Pb and Zn) and unpolluted soil, as well as dissolved oxygen (DO), pH, soil enzyme activity, and trace heavy metal dynamics in rhizosphere and nonrhizosphere soils. The results revealed that the growth of <em>A. hypochondriacus</em> was significantly inhibited under combined Cd, Pb, and Zn stress, with the most pronounced effects on root and branch biomass. Radial oxygen loss (ROL) and alkalization occurred in the roots of <em>A. hypochondriacus</em>, which were influenced primarily by root growth and environmental conditions. The enzyme activity patterns indicated that acid phosphatase (ACP), alkaline phosphatase (ALP), and β-glucosidase (BG) activities were primarily associated with the root system and that the activities of these enzymes were relatively low in nonrooted soil. Under heavy metal stress, the ACP, ALP and BG activities in the hotspots of soil increased. DO, pH, and soil enzyme activity were significantly correlated with the presence of heavy metals Cd, Pb, and Zn, influencing the mobilization mechanisms of trace metals in the rhizosphere. These findings lay the groundwork for understanding the impact of root-induced alterations in O₂, pH, and soil enzyme activity on the mobility of trace heavy metals in soil.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106067"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals distinct strategies of two potato cultivars against Phytophthora infestans and identifies StWRKY26 as a positive regulator of late blight resistance","authors":"Jingye Fu ,&nbsp;Ben Ma ,&nbsp;Li Zhu ,&nbsp;Yuhan Zhang ,&nbsp;Yuxin Li ,&nbsp;Shuaibin Tian ,&nbsp;Luyao Wang ,&nbsp;Hongjiang Liu ,&nbsp;Guocheng Wei ,&nbsp;Liping Yu ,&nbsp;Xueli Huang ,&nbsp;Changhe Wei ,&nbsp;Lang Yan ,&nbsp;Peihua Li ,&nbsp;Yan Liu ,&nbsp;Yangmei Wang ,&nbsp;Xiyao Wang ,&nbsp;Qiang Wang","doi":"10.1016/j.envexpbot.2025.106084","DOIUrl":"10.1016/j.envexpbot.2025.106084","url":null,"abstract":"<div><div>Late blight caused by <em>Phytophthora infestans</em> seriously threatens world’s potato production. Understanding the responsive mechanism of potato to <em>P. infestans</em> infection and exploring key regulators would be advantageous for improving the late blight resistance. Here, we firstly assessed the late blight resistance of 10 potato cultivars collected from Liangshan Yi Autonomous Prefecture and found that Chuan Liang Shu (CLS) is highly resistant, while Bu Wu Yu (BWY) is extremely susceptible. Comparative transcriptomics further revealed distinct mechanisms of CLS and BWY in response to <em>P. infestans</em> infection, in which CLS activates defense pathways rapidly, while BWY prioritizes energy towards growth and development pathways. Specifically, the up-regulated genes in CLS were largely enriched in oxidation-reduction process and SA-related pathways, resulting in activated ROS-scavenging systems and SA signaling. Clustering analysis for the expression patterns of transcription factors (TFs) demonstrates that the WRKY family members were significantly enriched in a cluster that is specifically induced in CLS after <em>P. infestans</em> infection. Silencing <em>StWRKY26,</em> one of the four WRKYs with higher induction levels, significantly compromise the intrinsic resistance against <em>P. infestans</em> in CLS. Our study enriches the knowledge of transcriptional responses of potato under <em>P. infestans</em> infection and provide candidate genes for breeding potato cultivars with strong late blight resistance.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106084"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PaGL1-like2 from Platanus acerifolia regulates anthocyanin accumulation and plant architecture","authors":"Yongkang Lu ,&nbsp;Yujuan Hu ,&nbsp;Yuqing Chen ,&nbsp;Manzhu Bao ,&nbsp;Yanping Zhang","doi":"10.1016/j.envexpbot.2024.106078","DOIUrl":"10.1016/j.envexpbot.2024.106078","url":null,"abstract":"<div><div>The London plane (<em>Platanus acerifolia</em>) is an important street tree known for its branching characteristics, making it valuable for garden applications. R2R3-MYB transcription factors play a crucial role in regulating plant development and metabolism. In this study, we isolated the <em>PaGL1-like2</em> gene, which encodes an R2R3-MYB transcription factor from <em>P. acerifolia</em>, and its gene function was validated. Overexpression of <em>PaGL1-like2</em> in tobacco altered the expression levels of structural genes in the phenylpropanoid biosynthesis pathway, resulting in changes in the accumulation of metabolites such as anthocyanins, flavonols, and lignin. Additionally, overexpression of this gene led to architectural changes in tobacco plants, including axillary bud outgrowth, leaf narrowing, and increased branching angles of leaf stalks. Transcriptome analysis indicated that <em>PaGL1-like2</em> may regulate the expression of genes involved in plant hormone signal transduction, as well as starch and sugar metabolism pathways, ultimately influencing tobacco plant architecture. These findings suggest that <em>PaGL1-like2</em> is involved in various biological processes due to its multifunctionality. Furthermore, this study provides valuable insights and strategies for the regulation of woody plant architecture.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106078"},"PeriodicalIF":4.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}