Endocrinologia japonica最新文献_第3页

In vitro monitoring activation by the ligands and specific DNA-binding of the glucocorticoid receptors. 体外监测糖皮质激素受体的配体激活和特异性dna结合。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.477

H Tanaka, E Fukawa, H Hirano, Y Makino, A Aoki, Y Morikawa, Y Takiyama, T Tani, I Makino

{"title":"In vitro monitoring activation by the ligands and specific DNA-binding of the glucocorticoid receptors.","authors":"H Tanaka, E Fukawa, H Hirano, Y Makino, A Aoki, Y Morikawa, Y Takiyama, T Tani, I Makino","doi":"10.1507/endocrj1954.39.477","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.477","url":null,"abstract":"The glucocorticoid receptor is a member of the steroid and thyroid hormone receptor superfamily and acts as a ligand-activated transcription factor. To reconstitute the molecular mechanisms underlying the cellular response to soluble receptor ligands, we have exploited a cell-free system that exhibits glucocorticoid-induced activation of the latent cytosolic glucocorticoid receptor to an active DNA-binding species. We demonstrate here that cytosol from a rat hepatoma cell, M1.19, contains glucocorticoid receptor-specific immunoreactivities and target DNA-binding activities. Moreover, specific DNA-binding activities of M1.19 cytosol were dose-dependently induced by dexamethasone treatment, and linearly correlated with the hormonal induction of chloramphenicol acetyltransferase activity at the corresponding concentrations. These results indicate that the cytosolic glucocorticoid receptor could be converted in a DNA-binding form under cell-free conditions and the ligand appears to play a crucial role in the direct control of the level of functional activity of a given ligand-receptor complex.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"477-83"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.477","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Simultaneous measurement of changes in the membrane potential and the intracellular Ca2+ concentration caused by somatostatin in human GH-producing pituitary tumor cells. 同时测量生长抑素引起的人gh -垂体肿瘤细胞膜电位和细胞内Ca2+浓度的变化。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.491

N Yamashita, K Takano, A Teramoto, K Tatakura, E Ogata

{"title":"Simultaneous measurement of changes in the membrane potential and the intracellular Ca2+ concentration caused by somatostatin in human GH-producing pituitary tumor cells.","authors":"N Yamashita, K Takano, A Teramoto, K Tatakura, E Ogata","doi":"10.1507/endocrj1954.39.491","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.491","url":null,"abstract":"Changes in the membrane potential and the intracellular Ca2+ concentration ([Ca2+]i) caused by somatostatin (SRIF) were simultaneously measured in human GH-producing pituitary tumor cells, by means of the nystatin-perforated whole cell clamp technique and Fura-2 AM. An application of 10(-8) M SRIF hyperpolarized the membrane and arrested Ca(2+)-dependent spontaneous action potentials. [Ca2+]i concurrently decreased during membrane hyperpolarization. When the membrane potential was clamped below the threshold for voltage-gated Ca2+ channels, [Ca2+]i decreased and SRIF did not further reduce [Ca2+]i. In cells which did not show spontaneous action potentials, SRIF hyperpolarized the membrane but it affected [Ca2+]i little. From these results it was concluded that the reduction in [Ca2+]i caused by SRIF was ascribed to the decrease in Ca2+ influx through voltage-gated channels during membrane hyperpolarization. The effect of SRIF on the voltage-gated Ca2+ channel current was also examined under the perforated whole cell clamp. SRIF (10(-8) M) inhibited the Ca2+ channel current to 80.8 +/- 15.4% (n = 5) of the control. Because SRIF-induced inhibition of the voltage-gated Ca2+ channel current was not prominent, it was considered that membrane hyperpolarization is the major cause of the reduction in [Ca2+]i in human GH-producing cells.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"491-7"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12534913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Simultaneous administration of TRH and sulpiride caused additive but not synergistic PRL responses in normal subjects. 同时给药TRH和舒必利在正常受试者中引起累加性而非增效性PRL反应。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.465

K Hanew, A Utsumi, A Sugawara, Y Shimizu, K Yoshinaga

引用次数: 2

Non-suppressed thyroidal radioactive iodine uptake (RAIU) in thyrotoxic phase in a case of subacute thyroiditis with thyroid-stimulating antibodies (TSAb). 亚急性甲状腺炎伴促甲状腺抗体(TSAb)的甲亢期非抑制甲状腺放射性碘摄取(RAIU)

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.469

S Nakamura, J Hattori, M Ishiyama-Takuno, H Shima, I Matsui, S Sakata

引用次数: 10

Secretory regulation of 19-hydroxyandrostenedione in normal man. 正常人19-羟基雄烯二酮的分泌调节。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.431

H Morita, T Mune, K Yasuda, L B Mercado-Asis, M Murayama, N Yamakita, S Miyazaki, K Miura

{"title":"Secretory regulation of 19-hydroxyandrostenedione in normal man.","authors":"H Morita, T Mune, K Yasuda, L B Mercado-Asis, M Murayama, N Yamakita, S Miyazaki, K Miura","doi":"10.1507/endocrj1954.39.431","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.431","url":null,"abstract":"To evaluate the secretory regulation of 19-hydroxyandrostenedione (19-OH-AD), its plasma concentration was measured before and after stimulation and inhibition tests for the ACTH-adrenal axis and the renin-angiotensin system in 50 normal subjects. Basal levels of plasma 19-OH-AD did not correlate with either those of plasma renin activity (PRA) or the plasma aldosterone concentration (PAC), but positively correlated with those of plasma cortisol. Plasma 19-OH-AD was stimulated by 0.25 mg ACTH-(1-24) and was suppressed by 1 mg dexamethasone (DEX) as were plasma cortisol and PAC. On the other hand, with 2-h standing alone or iv 40 mg furosemide plus 2-h standing, plasma 19-OH-AD and cortisol did not increase but PRA and PAC did. With iv furosemide plus 2-h standing with 3 mg DEX pretreatment, plasma 19-OH-AD and cortisol did not respond either, but PRA and PAC increased. With 25 mg oral captopril following 1-h standing with 3 mg DEX pretreatment, plasma 19-OH-AD and cortisol did not change but PAC decreased. These results indicate that the secretion of 19-OH-AD is mainly under the control of the ACTH-adrenal axis rather than the renin-angiotensin system.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"431-8"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.431","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A case of primary hyperparathyroidism due to an oxyphil cell adenoma. 嗜氧细胞腺瘤致原发性甲状旁腺功能亢进1例。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.499

T Shimada, K Higashi, K Kimura, T Shido, K Miura

引用次数: 2

Effect of 1-desamino-8-D-arginine vasopressin (DDAVP) on vasopressin release and blood pressure during hemorrhage. 1-去氨氨基-8- d -精氨酸抗利尿激素(DDAVP)对出血时抗利尿激素释放和血压的影响。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.517

K Shimizu, A Nakao

{"title":"Effect of 1-desamino-8-D-arginine vasopressin (DDAVP) on vasopressin release and blood pressure during hemorrhage.","authors":"K Shimizu, A Nakao","doi":"10.1507/endocrj1954.39.517","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.517","url":null,"abstract":"Whether or not 1-desamino-8-D-arginine-vasopressin (DDAVP) reduces blood pressure or affects the release of arginine vasopressin (AVP) and renin is controversial, although evidence suggests AVP and renin are important in maintaining blood pressure during hemorrhage. We therefore investigated the effect of DDAVP on endogenous release of AVP and renin and on blood pressure during hemorrhage in dogs. In the control group the hemorrhage was performed at a rate of 0.4 ml.kg-1.min-1 for 40 min from the femoral artery. The plasma AVP concentration and renin activity (PRA) increased progressively in response to the hemorrhage, from 7.5 +/- 0.5 to 40.3 +/- 7.3 pg.ml-1, and from 11.8 +/- 1.5 to 20.5 +/- 4.2 ng.ml-1.h-1, respectively, while blood pressure decreased slightly. In the DDAVP group, intravenous infusion of DDAVP (2.5 ng.kg-1.min-1 for 40 min) and hemorrhage were simultaneously performed. The plasma DDAVP concentration increased progressively to 218 +/- 21.0 pg.ml-1. There was no significant difference, however, between the control and DDAVP groups in the response of AVP, PRA and blood pressure. The results suggested that DDAVP may not affect the release of AVP and renin or blood pressure during hemorrhage.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"517-23"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Thyrotoxicosis with low serum total T3 level in patients with destructive thyroiditis and non-thyroidal illness. 破坏性甲状腺炎和非甲状腺疾病患者伴低血清总T3水平的甲状腺毒症。

Endocrinologia japonica Pub Date : 1992-08-01 DOI: 10.1507/endocrj1954.39.371

K Okamura, K Sato, H Ikenoue, S Osato, T Tokuyama, M Yoshinari, T Kuroda, M Fujishima

{"title":"Thyrotoxicosis with low serum total T3 level in patients with destructive thyroiditis and non-thyroidal illness.","authors":"K Okamura, K Sato, H Ikenoue, S Osato, T Tokuyama, M Yoshinari, T Kuroda, M Fujishima","doi":"10.1507/endocrj1954.39.371","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.371","url":null,"abstract":"The serum total T3 level, evaluated in 687 patients with thyrotoxicosis diagnosed by an elevated serum free T4 level and suppressed serum TSH level, was found to be high in 98.1% and normal in 1.9% of 592 patients with Graves' hyperthyroidism, and high in 75.8%, normal in 21.1% and low in 3.2% of 95 patients with destructive thyroiditis. Non-thyroidal illness was found in about a third of the patients with thyrotoxicosis and a normal serum total T3 level. The serum total T3 level was low with elevated serum thyroglobulin and reverse T3 levels in three patients with severe non-thyroidal illness, in whom the thyroidal radioactive iodine uptake was suppressed and the thyrotoxicosis resolved spontaneously with a normalization of the serum total T3 level after recovery from the destructive thyroiditis and non-thyroidal illness. It is therefore concluded that thyrotoxicosis with a low serum total T3 level, partially due to associated non-thyroidal illness, is more frequently found in patients with destructive thyroiditis than in those with Graves' hyperthyroidism.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 4","pages":"371-6"},"PeriodicalIF":0.0,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12618544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial effects of high daily dose bromocriptine treatment in Cushing's disease. 每日大剂量溴隐亭治疗库欣病的有益效果。

Endocrinologia japonica Pub Date : 1992-08-01 DOI: 10.1507/endocrj1954.39.385

L B Mercado-Asis, K Yasuda, M Murayama, T Mune, H Morita, K Miura

{"title":"Beneficial effects of high daily dose bromocriptine treatment in Cushing's disease.","authors":"L B Mercado-Asis, K Yasuda, M Murayama, T Mune, H Morita, K Miura","doi":"10.1507/endocrj1954.39.385","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.385","url":null,"abstract":"Treatment with a high daily dose bromocriptine was evaluated in 6 Cushing's disease patients (4 females and 2 males; aged 23 to 56 years). The highest doses administered were 40 mg to patient 1, 55 mg to patient 2, 35 mg to patient 3, 25 mg to patient 4, 25 mg to patient 5, and 17.5 mg to patient 6. The former 3 cases, 2 (patients 1 and 2) of whom were previously reported and further followed up, showed clinical and biochemical improvement with the regimen. Patient 1 who obtained remission with 40 mg/day has been on remission for further 14 months with a total of 36 months. Patient 2, who had a reduction in pituitary tumor size with 35 mg daily, relapsed thereafter. The therapy, however, resolved the paradoxical responses of plasma ACTH and cortisol to arginine. Readministration of bromocriptine resulted into another clinical and biochemical improvement with 45 to 55 mg/day. Patient 3, a relapsed case after a remission with reserpine plus pituitary irradiation, showed an improvement in the 24-h urinary free cortisol excretion with 35 mg/day. Patient 4 was the only case who had a marked decrease in plasma cortisol (basal; 16.3, nadir; 1.9 micrograms/dl) after a single-dose bromocriptine test among the 5 cases tested. The patient had favorable response with 25 mg/day for 2 months but the dose was not increased after an escape. Patient 5 received the drug in 4 occasions, 7.5 to 25 mg/day, in combination with several agents, which failed to induce clinical remission. The last patient did not respond to a maximum dose of 17.5 mg/day. These observations suggest that, regardless of the result of a single-dose bromocriptine test, treatment with a high daily dose of bromocriptine, 35 mg or more, may be necessary to obtain a favorable clinical response and normal cortisol secretion.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 4","pages":"385-95"},"PeriodicalIF":0.0,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12506516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

ACTH increases expression of c-fos, c-jun and beta-actin genes in the dexamethasone-treated rat adrenals. ACTH增加地塞米松处理大鼠肾上腺中c-fos、c-jun和-肌动蛋白基因的表达。

Endocrinologia japonica Pub Date : 1992-08-01 DOI: 10.1507/endocrj1954.39.377

M Ohno, H Seo, T Imai, Y Murata, N Miyamoto, Y Satoh, H Funahashi, H Takagi, N Matsui

{"title":"ACTH increases expression of c-fos, c-jun and beta-actin genes in the dexamethasone-treated rat adrenals.","authors":"M Ohno, H Seo, T Imai, Y Murata, N Miyamoto, Y Satoh, H Funahashi, H Takagi, N Matsui","doi":"10.1507/endocrj1954.39.377","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.377","url":null,"abstract":"Our recent finding that ACTH increases c-fos mRNA in the adrenal gland of hypophysectomized rats indicates that the gene product FOS may play an important role(s) in mediating the action of ACTH. However, hypophysectomy employed in that study causes the disappearance of trophic hormones other than ACTH and may modify the effect of ACTH. Thus, in the present investigation, dexamethasone-treated rats were used. Since FOS functions only when it dimerizes with JUN (the product of c-jun gene), the changes in the levels of c-fos and c-jun mRNAs were studied together with that of beta-actin mRNA which is also affected by ACTH. Northern blot analysis was employed to determine the mRNA levels. It was demonstrated that ACTH increases the mRNAs coding c-fos and c-jun in the adrenal glands of dexamethasone-treated, ACTH-suppressed rats. The c-fos mRNA was not detectable before ACTH administration. After ACTH administration, the mRNA levels were transiently increased, the maximum level being observed at 30 min after ACTH. At 180 min post ACTH, the level returned to the unstimulated level. The mRNA coding c-jun was detectable before ACTH administration and it also increased rapidly after ACTH with maximal stimulation at 30 min. However, the mRNA level at 180 min post ACTH was still higher than the unstimulated level. The changes in beta-actin mRNA were approximately the same as those of c-jun mRNA. These results suggest that increased expression of c-fos, c-jun and beta-actin genes by ACTH may play an important role in mediating its action on the adrenals.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 4","pages":"377-83"},"PeriodicalIF":0.0,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.377","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12454985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5