Endocrinologia japonica最新文献_第10页

A case of Graves' disease with false hyperthyrotropinemia who developed silent thyroiditis. Graves病合并假甲状腺球蛋白高血症并发隐匿性甲状腺炎1例。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.667

M Iitaka, J Ishii, N Ishikawa, H Yoshimura, N Momotani, H Saitou, K Ito

引用次数: 11

Clinical evaluation of accuracy in determining serum free thyroxine and free triiodothyronine in patients with non-thyroidal illness: immunoglobulin effect on T3/TBG ratio and T4/TBG ratio. 非甲状腺疾病患者血清游离甲状腺素和游离三碘甲状腺原氨酸测定准确性的临床评价:免疫球蛋白对T3/TBG比值和T4/TBG比值的影响

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.633

T Hashimoto, K Kawai, M Nishibu, S Fujita, H Horita

{"title":"Clinical evaluation of accuracy in determining serum free thyroxine and free triiodothyronine in patients with non-thyroidal illness: immunoglobulin effect on T3/TBG ratio and T4/TBG ratio.","authors":"T Hashimoto, K Kawai, M Nishibu, S Fujita, H Horita","doi":"10.1507/endocrj1954.38.633","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.633","url":null,"abstract":"We examined the effect of endogenous immunoglobulins (G, A and M) and albumin on the measurement of thyroid hormones by different methods, including a new non-isotopic immunoassay of free thyroxine (FT4) and free triiodothyronine (FT3), in a large number of patients with non-thyroidal illness (NTI). Variations in serum protein concentrations can affect the results of radioimmunoassay of human thyroid hormones and thyroxine binding globulin (TBG). Our data revealed that in patients with non-thyroidal illness, when fluctuations in serum gamma-globulin occurred the T3/TBG and T4/TBG ratios altered. Consequently, when patients are suffering from non-thyroidal illness with changing gamma-globulin levels, clinical scientists should take care when they use T3/TBG and T4/TBG ratios as a substitute for FT3 or FT4 estimation. We found FT4 and FT3 (determined with Amerlex-M kits) T3 and the T3/TBG ratio were altered inversely due to the difference in the serum gamma-globulin levels. A recently developed enhanced luminescence enzyme immunoassay for FT3 and FT4 (Amerlite FT3 and FT4 kits) provides more reliable and accurate results, because of its resistance to interference, especially from albumin and gamma-globulin.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"633-9"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.633","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12984666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effects of a beta 3-adrenoceptor agonist, BRL 26830A, on insulin and glucagon release in mice. β 3-肾上腺素能受体激动剂BRL 26830A对小鼠胰岛素和胰高血糖素释放的影响。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.641

T Yoshida, N Hiraoka, M Kondo

{"title":"Effects of a beta 3-adrenoceptor agonist, BRL 26830A, on insulin and glucagon release in mice.","authors":"T Yoshida, N Hiraoka, M Kondo","doi":"10.1507/endocrj1954.38.641","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.641","url":null,"abstract":"The beta 3-adrenoceptor agonist, BRL 26830A, which is not inhibited by either beta 1 or beta 2-selective antagonists, has been shown to possess anti-obesity and anti-diabetic actions. However, the effects of this agent on insulin and glucagon release have not yet been substantiated. Therefore, we tested the hypothesis that BRL 26830A promotes insulin and glucagon secretion via beta 3 receptors on pancreatic islet B and A cells. In ICR mice fasted for 48 h, BRL 26830A significantly stimulated insulin secretion from 5 min after administration, markedly decreased blood glucose levels from 30 min after administration, and significantly increased glucagon secretion from 30 min after administration. The administration of a non-selective beta-receptor antagonist, at a dose of 50 mg/kg, 30 min prior to BRL 26830A injection completely abolished the effects induced by BRL 26830A. However, the administration of a beta 1-selective antagonist at doses of 50 or 100 mg/kg did not produce any significant effects. On the action of BRL 26830A, whereas the administration of a beta 2-selective antagonist at 50 mg/kg, a near maximal effective dose, partially abolished the effects of BRL 26830A. BRL 26830A had no effect on insulin, glucagon, or glucose levels in streptozocin (STZ) diabetic mice fasted for 48 h. These results suggest that, in mice, BRL 26830A may promote insulin secretion mainly via beta 3 receptors and partially via beta 2 receptors on pancreatic-islet B cells, and that glucagon may be secreted as the result of hypoglycemia induced by this agent.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"641-6"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.641","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12853419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Disturbance of thyroidal iodine metabolism in BB/W rat. BB/W大鼠甲状腺碘代谢紊乱。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.647

S Miura, Y Hara, M Iitaka, N Fukasawa, K Hase, M Urabe, Y Sakatsume, M Yanagisawa, J Ishii, S Kawazu

{"title":"Disturbance of thyroidal iodine metabolism in BB/W rat.","authors":"S Miura, Y Hara, M Iitaka, N Fukasawa, K Hase, M Urabe, Y Sakatsume, M Yanagisawa, J Ishii, S Kawazu","doi":"10.1507/endocrj1954.38.647","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.647","url":null,"abstract":"To investigate the thyroid function in Bio-Breeding Worcester (BB/W) rats, we have examined the iodine metabolism, serum TSH and thyroid hormone levels in 8- and 16-week-old BB/W and normal Wistar (W) rats. At 8 weeks of age, serum TSH levels were significantly higher in BB/W rats than in W rats, although there was no difference in the serum levels of free T3 and free T4. Furthermore, the thyroidal radioactive iodine incorporation at 48 h was significantly lower in BB/W rats, suggesting that they might have some defects in iodine organification. At 16 weeks of age, serum TSH levels were also significantly higher in BB/W rats than in W rats. Furthermore, serum TSH levels in 16-week-old BB/W rats were significantly higher than in 8-week-old BB/W rats. The thyroid weight was significantly greater in BB/W rats, probably due to the increased serum TSH. The thyroidal radioactive iodine uptake at 48 h and the iodine content in the thyroid homogenates were significantly lower in BB/W rats. These results suggest that BB/W rats have some defect in iodine metabolism resulting in impaired thyroid hormone synthesis.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"647-53"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12984667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Nucleotide sequences of cDNA clones, pSv-1 and pSv-2, hybridizing to androgen-stimulated mRNAs in rat seminal vesicles. 大鼠精囊中与雄激素刺激mrna杂交的cDNA克隆pSv-1和pSv-2的核苷酸序列。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.577

M Izawa

引用次数: 3

Salmon calcitonin induces pituitary tumor in rats. 鲑鱼降钙素诱导大鼠垂体瘤。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.705

J Ishii, S Katayama, A Itabashi, M Takahama, S Kawazu

{"title":"Salmon calcitonin induces pituitary tumor in rats.","authors":"J Ishii, S Katayama, A Itabashi, M Takahama, S Kawazu","doi":"10.1507/endocrj1954.38.705","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.705","url":null,"abstract":"Calcitonin is widely used in the treatment of post-menopausal osteoporosis. The present study was designed to investigate the effects of salmon calcitonin (SCT) on the incidence of the pituitary tumors in Sprague-Dawley (SD) rats. Subcutaneous injections of SCT at a dose of 160 IU/kg/day for 6 months reduced body weight and induced one pituitary hyperplasia and three pituitary adenomas in 4 of 5 animals, while 5 controls did not show any changes. Prolactin-positive cells were located at the periphery of the affected pituitaries adjacent to the prolactin-negative adenomas. In addition, serum concentrations of prolactin and TSH were lower than in the controls, although serum calcium or LH levels were not significantly different from the controls. Among 7 animals treated with SCT for 6 months followed by no medication for another 6 months, 5 adenomas were detected, one of which had invasive growth toward the adjacent tissue, whereas only one adenoma was found in 9 controls. These results suggest that SCT administration at a high dose may induce the formation of pituitary adenoma, or may accelerate the development of spontaneous pituitary adenomas, some of which show frequent mitotic figures and invasive growth into the surrounding tissue, possibly resulting in malignant transformation. This indicates the need for caution in considering whether calcitonin injections into patients with osteoporosis as well as Paget's disease may induce such pituitary tumors.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"705-9"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12985263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Lack of PTC gene (ret proto-oncogene rearrangement) in human thyroid tumors. PTC基因(ret原癌基因重排)在人甲状腺肿瘤中的缺失。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.627

H Namba, S Yamashita, H C Pei, N Ishikawa, M C Villadolid, T Tominaga, H Kimura, M Tsuruta, N Yokoyama, M Izumi

{"title":"Lack of PTC gene (ret proto-oncogene rearrangement) in human thyroid tumors.","authors":"H Namba, S Yamashita, H C Pei, N Ishikawa, M C Villadolid, T Tominaga, H Kimura, M Tsuruta, N Yokoyama, M Izumi","doi":"10.1507/endocrj1954.38.627","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.627","url":null,"abstract":"PTC gene, which is derived from the rearranged form of the ret proto-oncogene, was originally discovered in human thyroid papillary carcinomas. This gene has been thought to act as a tumorigenetic factor in thyroid carcinoma, although the action of PTC oncogene products is still unknown. To study the frequency of the PTC gene present in human thyroid carcinomas, we investigated four cell lines derived from thyroid carcinoma and 22 thyroid tumor tissue specimens. The reverse transcriptase-polymerase chain reaction (RT-PCR) method was performed to detect putative PTC mRNA. The presence of the PTC gene in genomic DNA was analyzed by Southern blot hybridization. PTC mRNA was detected by the RT-PCR method in only one papillary carcinoma cell line (TPC-1 cell). Southern gel analysis confirmed the rearrangement of the ret proto-oncogene in this cell line. In the other three cell lines and 22 tumor tissue specimens, however, neither the PTC gene or mRNA was detected. These results demonstrate that the prevalence of the PTC gene in thyroid tumor is low and may not be essential for human thyroid tumorigenesis. That our present results conflict with previous reports may be due to general differences in genetic background among races.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"627-32"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12984665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Isolation and characterization of cDNA clones for castration-induced mRNAs in the rat ventral prostate. 大鼠腹侧前列腺去势诱导mrna cDNA克隆的分离与鉴定。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.619

Y Hoshikawa, Y Satoh, S Ichii

{"title":"Isolation and characterization of cDNA clones for castration-induced mRNAs in the rat ventral prostate.","authors":"Y Hoshikawa, Y Satoh, S Ichii","doi":"10.1507/endocrj1954.38.619","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.619","url":null,"abstract":"To identify gene products involved in castration-induced involution of the rat ventral prostate, we constructed a subtraction cDNA library of the ventral prostate from rats castrated for 48 h. The library was screened with subtracted cDNA probes enriched for sequences with a low copy number expressed in intact or castrated rats. As a result of differential screening, 48 cDNA clones representing 10 different induced mRNAs were isolated. The time course of these mRNA inductions after castration was examined. Within the first 24 h after castration, the level of mRNAs for these cDNA clones was significantly increased and it reached its peak by 48-72 h after castration. Although mRNAs for these cDNA clones were expressed in various tissues from intact rats, an increase in mRNA as a response to castration was observed only in the ventral prostate. Partial sequence analyses of the 10 cDNA clones indicate that three cDNA clones represent rat glutathione S-transferase Yb-1, Yb-2 and Yb-3 subunit mRNA sequences, but for others respective homologues could not be found in a search of the GenBank database (release 67).","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"619-26"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12984734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Radioimmunoassay with heterologous antibody (hetero-antibody RIA): utilization of highly cross-reactive antibody present in polyclonal antiserum. 异源抗体放射免疫测定(hetero-antibody RIA):利用多克隆抗血清中存在的高交叉反应抗体。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.673

A Iwasawa, H Hayashi, Z Itoh, K Wakabayashi

{"title":"Radioimmunoassay with heterologous antibody (hetero-antibody RIA): utilization of highly cross-reactive antibody present in polyclonal antiserum.","authors":"A Iwasawa, H Hayashi, Z Itoh, K Wakabayashi","doi":"10.1507/endocrj1954.38.673","DOIUrl":"https://doi.org/10.1507/endocrj1954.38.673","url":null,"abstract":"To develop a homologous radioimmunoassay (RIA) for a hormone of a small or rare animal often meets difficulty in collecting a large amount of purified antigen required for antibody production. On the other hand, to employ a heterologous RIA to estimate the hormone often gives poor sensitivity. To overcome this difficulty, a \"hetero-antibody\" RIA was studied. In a hetero-antibody RIA system, a purified preparation of a hormone is used for radioiodination and standardization and a heterologous antibody to the hormone is used for the first antibody. Canine motilin and rat LH were selected as examples, and anti-porcine motilin and anti-hCG, anti-hCG beta or anti-ovine LH beta was used as the heterologous antibody. The sensitivities of the hetero-antibody RIAs were much higher than those of heterologous RIAs in any case, showing that these hetero-antibody RIA systems were suitable for practical use. To clarify the principle of hetero-antibody RIA, antiserum to porcine motilin was fractionated on an affinity column where canine motilin was immobilized. The fraction bound had greater constants of affinity with both porcine and canine motilins than the rest of the antibody fractions. This fraction also reacted with a synthetic peptide corresponding to the C-terminal sequence common to porcine and canine motilins in a competitive binding test with labeled canine motilin. These results suggest that an antibody population having high affinity and cross-reactivity is present in polyclonal antiserum and indicate that the population can be used in hetero-antibody RIA at an appropriate concentration.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"38 6","pages":"673-81"},"PeriodicalIF":0.0,"publicationDate":"1991-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.38.673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12984670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Involvement of protein kinase C in angiotensin II-mediated release of 12-hydroxyeicosatetraenoic acid in bovine adrenal glomerulosa cells. 蛋白激酶C参与血管紧张素ii介导的牛肾上腺肾小球细胞中12-羟基二十碳四烯酸的释放。

Endocrinologia japonica Pub Date : 1991-12-01 DOI: 10.1507/endocrj1954.38.611

H Shibata, I Kojima

引用次数: 5