Endocrinologia japonica最新文献

The effect of DHAS on steroidogenesis of the human corpus luteum. DHAS对人黄体甾体生成的影响。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.539

T Iwai, S Taii, H Ozasa, T Mori

{"title":"The effect of DHAS on steroidogenesis of the human corpus luteum.","authors":"T Iwai, S Taii, H Ozasa, T Mori","doi":"10.1507/endocrj1954.39.539","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.539","url":null,"abstract":"To examine whether or not dehydroepiandrosterone sulfate (DHAS) is a substrate for steroidogenesis in the corpus luteum, we studied 17 women in the luteal phase, the follicular phase, and after castration. Following suppression of adrenal function with dexamethasone, DHAS was administered intravenously and the serum levels of DHAS, dehydroepiandrosterone (DHA), androstenedione (ADS), testosterone (T), 17 beta-estradiol (E2) and progesterone (P) were measured serially for 24 h. An obvious increase in the serum levels of all steroids except for E2 and P was observed in each subject for at least 8 h after DHAS administration. To evaluate the effect of DHAS on the serum levels of the steroid hormones, the integrated response area (IRA) was calculated for each hormone in all the subjects. The IRA values for ADS, T and E2 (at 2 and 4 h) in the luteal phase group were significantly higher than in the other DHAS treated groups, and the IRA values for DHA and P tended to be higher than in the other groups. These results suggest that the corpus luteum utilizes serum DHAS as a substrate for steroidogenesis.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"539-48"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Thyroxine-binding globulin variant (TBG-Kumamoto): identification of a point mutation and genotype analysis of its family. 甲状腺素结合球蛋白变异(TBG-Kumamoto):一个点突变的鉴定及其家族基因型分析。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.577

T Shirotani, H Kishikawa, N Wake, N Miyamura, Y Hashimoto, S Motoyoshi, K Yamaguchi, M Shichiri

{"title":"Thyroxine-binding globulin variant (TBG-Kumamoto): identification of a point mutation and genotype analysis of its family.","authors":"T Shirotani, H Kishikawa, N Wake, N Miyamura, Y Hashimoto, S Motoyoshi, K Yamaguchi, M Shichiri","doi":"10.1507/endocrj1954.39.577","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.577","url":null,"abstract":"Thyroxine-binding globulin (TBG) is the major thyroid hormone transport protein. Several inherited TBG variants resulting in partial or complete TBG deficiencies have been shown to be caused by either one or two nucleotide substitutions, or one nucleotide deletion in the coding regions of the TBG gene. In this report, a Japanese female patient (proband) with hyperthyroid state, whose lower TBG levels did not return to normal under the euthyroid state after treatment was examined. Genomic DNA samples from the proband with thyroxine-binding globulin deficiency (termed TBG-Kumamoto) and her family were subjected to the polymerase chain reaction, and the generated DNA fragments were sequenced. A single nucleotide substitution in the codon for the amino acid 363 of native TBG molecule (CCT to CTT) was found, resulting in the replacement of proline by leucine. It was revealed that the proband was a heterozygote and her father was a hemizygote. The mutation was confirmed by the allele-specific amplification of genomic DNAs from the proband and her father using oligonucleotide primers of normal or mutant residues at the 3' position in the polymerase chain reaction. These results indicate that the abnormality of TBG-Kumamoto is the consequence of this mutation. Genetically, this point mutation observed in TBG-Kumamoto might be classified as a new type of TBG deficiency.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"577-84"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.577","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Usefulness and limitation of measurement of insulin-like growth factor binding protein-3 (IGFBP-3) for diagnosis of growth hormone deficiency. 胰岛素样生长因子结合蛋白-3 (IGFBP-3)在生长激素缺乏症诊断中的有效性和局限性

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.585

Y Hasegawa, T Hasegawa, T Aso, S Kotoh, Y Tsuchiya, O Nose, Y Ohyama, K Araki, T Tanaka, S Saisyo

{"title":"Usefulness and limitation of measurement of insulin-like growth factor binding protein-3 (IGFBP-3) for diagnosis of growth hormone deficiency.","authors":"Y Hasegawa, T Hasegawa, T Aso, S Kotoh, Y Tsuchiya, O Nose, Y Ohyama, K Araki, T Tanaka, S Saisyo","doi":"10.1507/endocrj1954.39.585","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.585","url":null,"abstract":"To analyze the utility of insulin-like growth factor binding protein-3 (IGFBP-3) radioimmunoassay for diagnosis of growth hormone deficiency (GHD) we measured IGFBP-3 in sera from normal children, short children and patients with GHD. The sensitivity (true positive ratio) of IGFBP-3 for complete GHD (cGHD) was 93%, while the specificity (true negative ratio) for normal short children (NS) was 88%. In contrast, the sensitivity of IGFBP-3 for partial GHD (pGHD) was only 43%. The poor discrimination between patients with pGHD and NS may be the result of their relatively similar GH level, as compared to cGHD, or due to the limitations of GH stimulation tests. The specificity of IGFBP-3 for NS was excellent in children of all ages: less than 10 years old (87%) and older than 10 (88%). However, sensitivity for GHD was good for children less than 10 years old (84%) but poor for children older than 10 (64%). IGFBP-3 may be less sensitive for diagnosing GHD in older children because IGFBP-3 levels may also increase during puberty due to mechanisms independent of the GH-IGF-I axis.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"585-91"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.585","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Anti-eye muscle antibodies and hypothyroid Graves' disease: a case report. 抗眼肌抗体与甲状腺功能减退Graves病1例

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.593

Y Hiromatsu, M Sato, K Tanaka, K Nonaka, K Kojima, K Sato, S Kurose, T Hoshino, A Nakashima

{"title":"Anti-eye muscle antibodies and hypothyroid Graves' disease: a case report.","authors":"Y Hiromatsu, M Sato, K Tanaka, K Nonaka, K Kojima, K Sato, S Kurose, T Hoshino, A Nakashima","doi":"10.1507/endocrj1954.39.593","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.593","url":null,"abstract":"We report the case of a 70-year-old man who developed hypothyroidism associated with TSH receptor antibodies and severe ophthalmopathy during lithium therapy. He had received lithium therapy for more than 20 years for manic depression, when ophthalmopathy (class VI of the American Thyroid Association classification) and mild hypothyroidism developed. Orbital magnetic resonance imaging indicated marked enlargement of the superior, medial and inferior rectus muscles in the left eye. He had anti-eye muscle antibodies in his serum, detected by Western blotting and quantified by chromatoscanning, as well as anti-TSH receptor antibodies. He was treated with supplementation of levothyroxine and four cycles of methylprednisolone pulse therapy. After the pulse therapy, both anti-eye muscle antibodies and anti-TSH receptor antibodies decreased and disappeared in parallel with the improvement in eye symptoms and signs. These observations suggest the importance of anti-eye muscle antibodies as clinical markers in the development of thyroid-associated ophthalmopathy.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"593-600"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.593","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12535484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Relationship between inhibitor of extrathyroidal 5'-deiodinase activity and serum free fatty acid in children with nonthyroidal illness and acute ketosis. 非甲状腺疾病和急性酮症患儿甲状腺外5′-脱碘酶活性抑制剂与血清游离脂肪酸的关系

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.525

H Hashimoto, N Igarashi, T Sato, T Hashimoto

{"title":"Relationship between inhibitor of extrathyroidal 5'-deiodinase activity and serum free fatty acid in children with nonthyroidal illness and acute ketosis.","authors":"H Hashimoto, N Igarashi, T Sato, T Hashimoto","doi":"10.1507/endocrj1954.39.525","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.525","url":null,"abstract":"To clarify whether serum free fatty acid (FFA) is an inhibitor of extrathyroidal conversion (IEC) of thyroxine (T4) to thyronine (T3), we measured the concentration of FFA, IEC activity and thyroid hormones in normal subjects, acute ketotic children and children with low T3 syndrome due to nonthyroidal illness (NTI). Iodothyronine (I) 5'-deiodinase activity was assayed with reverse triiodothyronine (rT3) as substrate and liberated 125I-was measured. The IEC was determined by the inhibition of I 5'-deiodination by ether extract of sera or standard oleate solution. IEC values were represented as mM oleate. The serum concentration of FFA was 0.470 +/- 0.117 (SD) mM in 11 normal subjects, and it was significantly higher (1.242 +/- 0.248 mM; P < 0.01) in 10 acute ketotic children and in 7 samples from 6 NTI children (0.904 +/- 0.530 mM; P < 0.05). In contrast, there was no difference in IEC among three groups (normal subject, 0.451 +/- 0.069 mM; acute ketosis, 0.437 +/- 0.040 mM; NTI, 0.465 +/- 0.224 mM). No correlations were found between IEC activity and the serum FFA concentration or thyroid hormones in 28 samples from three groups. The sequential changes in serum thyroid hormones, FFA and IEC in 3 of 6 NTI children revealed no consistent relationship. Furthermore, one NTI child had significantly high IEC (> 1.000 mM) but its serum FFA (1.182 mM) was below the mean value for the acute ketotic group. These results indicate that 1) many NTI patients may bear no relation to IEC and 2) IEC may not be caused by serum FFA only but includes several factors.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"525-31"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12469224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transient extreme insulin resistance in shock during diabetic ketoacidosis. 糖尿病酮症酸中毒休克时的短暂性极端胰岛素抵抗。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.571

H Yokoyama, T Wasada, Y Shimizu, H Yoshino, S Hasumi, Y Omori

{"title":"Transient extreme insulin resistance in shock during diabetic ketoacidosis.","authors":"H Yokoyama, T Wasada, Y Shimizu, H Yoshino, S Hasumi, Y Omori","doi":"10.1507/endocrj1954.39.571","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.571","url":null,"abstract":"Transient extreme insulin resistance was encountered during an episode of diabetic ketoacidosis (DKA) in an insulin-treated diabetic patient. On admission, the plasma glucose level was 1241 mg dl-1 and arterial blood pH 6.895 with HCO3- 4.7 mEql-1. An intravenous bolus injection of 20 units, followed by continuous infusion of 20 units h-1 of short-acting regular human insulin, was instituted. Ischemic myocardial changes were noted on the initial electrocardiogram, therefore fluid replacement was limited to 1,000 ml of 0.9% saline solution in the first hour. As the plasma glucose level declined by only 203 mg dl-1 (41 mg dl-1 h-1) in the first 5 h, the insulin dose was doubled every 2 h. At hour 4, the patient developed circulatory shock which required vasopressor support and respiratory assistance. A plasma glucose level of 300 mg dl-1 was not achieved until the total dosage of insulin amounted to 91,580 units at hour 25. Insulin resistance was not observed from that point on. The patient had neither insulin antibodies nor anti-insulin receptor antibodies in serologic testing. The insulin binding characteristics of the patient's erythrocytes were similar to those from healthy controls both with and without experimental acidosis and with a high level of beta-hydroxybutyrate. Among multiple potential factors, the severe shock associated with DKA has been considered as a primary cause of the transient severe insulin resistance in this case.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"571-6"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Source of prolactin in human follicular fluid. 人卵泡液中催乳素的来源。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.601

M Ohwaki, N Suganuma, H Seo, A Nawa, F Kikkawa, O Narita, N Matsui, Y Tomoda

引用次数: 8

Immunohistochemical evidence that rat FSH cells contain beta II-subspecies of protein kinase C. 免疫组织化学证据表明大鼠FSH细胞含有蛋白激酶C的β ii亚种。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.609

M Ohmichi, K Hirota, K Koike, A Miyake, O Tanizawa, M Sato, M Tohyama

引用次数: 1

Clinical significance of adrenal computed tomography in Addison's disease. 肾上腺计算机断层扫描在Addison病中的临床意义。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.563

Z H Sun, K Nomura, S Toraya, M Ujihara, N Horiba, T Suda, T Tsushima, H Demura, A Kono

{"title":"Clinical significance of adrenal computed tomography in Addison's disease.","authors":"Z H Sun, K Nomura, S Toraya, M Ujihara, N Horiba, T Suda, T Tsushima, H Demura, A Kono","doi":"10.1507/endocrj1954.39.563","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.563","url":null,"abstract":"Adrenal computed tomographic (CT) scanning was conducted in twelve patients with Addison's disease during the clinical course. In tuberculous Addison's disease (n = 8), three of four patients examined during the first two years after disease onset had bilaterally enlarged adrenals, while one of four had a unilaterally enlarged one. At least one adrenal gland was enlarged after onset in all six patients examined during the first four years. Thereafter, the adrenal glands may atrophy bilaterally, in contrast to adrenal glands in idiopathic Addison's disease, which atrophy bilaterally from disease onset (n = 2). Adrenal calcification was a less sensitive clue in tracing pathogenesis, i.e., adrenal calcification was observed in five of eight patients with tuberculous Addison's disease, but not in idiopathic patients. Thus, adrenal CT scanning could show the etiology of Addison's disease (infection or autoimmunity) and the phase of Addison's disease secondary to tuberculosis, which may be clinically important for initiating antituberculous treatment.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"563-9"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.563","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Characterization of parathyroid hormone-related protein in the human term placenta. 人足月胎盘中甲状旁腺激素相关蛋白的特征。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.555

E Ishikawa, H Katakami, H Hidaka, Y Ushiroda, T Ikeda, T Ikenoue, S Matsukura

{"title":"Characterization of parathyroid hormone-related protein in the human term placenta.","authors":"E Ishikawa, H Katakami, H Hidaka, Y Ushiroda, T Ikeda, T Ikenoue, S Matsukura","doi":"10.1507/endocrj1954.39.555","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.555","url":null,"abstract":"To characterize parathyroid hormone-related protein (PTHrP) in the human placenta, we measured PTHrP-like immunoreactivity (PRP-LI) in the term placenta and studied the elution profiles of placental tissue extracts on Sephadex G-75 chromatography with a specific RIA. We also examined the gene expression of PTHrP mRNA by Northern blot analysis and the localization of PRP-LI in the placenta by immunohistochemistry. The amount of PRP-LI in placental extracts (n = 7) was 20.9 +/- 2.2 pg/g wet tissue (mean +/- SE). Dilution curves of placental tissue ran parallel to those of synthetic PTHrP (1-34) standards. Sephadex G-75 gel chromatography demonstrated two major PRP-LI peaks; the first peak was eluted around the molecular size between 10 kilodaltons (Kda) and 20 Kda and the other around 5 Kda. Northern blot analysis of PTHrP mRNA extracted from placental tissues showed a major hybridization signal around 18S. PTHrP immunohistochemistry showed PRP-LI staining in the cytoplasm of syncytiotrophoblasts and stroma cells (Hofbauer cells) in the term placenta. These results suggest that syncytiotrophoblasts and stroma cells in the term placenta synthesize PTHrP in two major molecular forms, 10 Kda-20 Kda and around 5 Kda.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"555-61"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.555","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12468349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12