Endocrinologia japonica最新文献_第2页

Influence of corticosterone acetate on the spleen in intact and ovariectomized rats. 醋酸皮质酮对完整和去卵巢大鼠脾脏的影响。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.549

G Valli, M Gladson, N Srinivasan, P Govindarajulu

{"title":"Influence of corticosterone acetate on the spleen in intact and ovariectomized rats.","authors":"G Valli, M Gladson, N Srinivasan, P Govindarajulu","doi":"10.1507/endocrj1954.39.549","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.549","url":null,"abstract":"Increasing evidence of the interaction of glucocorticoids and ovarian steroids prompted the current study. Effects of exogenously administered corticosterone acetate (3.5 mg/100 g b.w/day for one week) were examined on splenic nucleic acids, protein, lactate, and on lactate dehydrogenase (LDH) specific activity and its isozymes in ovariectomized and ovary-intact Wistar rats (65-75 days old). Ovariectomy resulted in no significant change in the parameters studied except DNA which increased significantly. The administration of corticosterone to these rats did not produce any remarkable change in the ovariectomy caused increase in splenic DNA content. Nevertheless, it decreased the ratio of heart type subunits (H)/muscle type subunits (M) [H/M] of LDH isozymes. In the case of ovary-intact rats, corticosterone produced an increase in the concentration of splenic lactate but a decrease in the H/M ratio. Exogenously administered corticosterone exerts selective synergistic interaction with ovarian hormones on splenic lactate. The specific activity of LDH and the concentrations of RNA and protein remained unchanged during the interaction between ovarian hormones and corticosterone.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"549-54"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No inhibitory effects of gestrinone and medroxyprogesterone acetate on the estrogen production by ovaries of hypophysectomized rats stimulated by gonadotropins. 孕酮和醋酸甲孕酮对促性腺激素刺激下去垂体大鼠卵巢产生雌激素无抑制作用。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.615

T Mizutani, M Sakata, A Miyake, O Tanizawa, N Terada, K Matsumoto, N Terakawa

{"title":"No inhibitory effects of gestrinone and medroxyprogesterone acetate on the estrogen production by ovaries of hypophysectomized rats stimulated by gonadotropins.","authors":"T Mizutani, M Sakata, A Miyake, O Tanizawa, N Terada, K Matsumoto, N Terakawa","doi":"10.1507/endocrj1954.39.615","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.615","url":null,"abstract":"The in vivo effects of gestrinone (R2323) and medroxyprogesterone acetate (MPA) on the estrogen production by rat ovaries were investigated. Hypophysectomized immature female rats treated with 2.5 or 5 IU of pregnant mare serum gonadotropin (PMS) were daily given vehicle only, gestrinone (0.5 mg/kg body weight) or MPA (10 mg/kg body weight), and the activities of 3 beta-hydroxysteroid dehydrogenase, 17 alpha-hydroxylase, 17, 20-lyase, 17 beta-hydroxysteroid dehydrogenase and aromatase in ovaries of these rats were measured. Gestrinone suppressed the 3 beta-hydroxysteroid dehydrogenase activity and increased activities of 17 alpha-hydroxylase, 17, 20-lyase and aromatase in ovaries stimulated by 5 IU of PMS, while MPA suppressed activities of 17 alpha-hydroxylase and aromatase in these ovaries. On the other hand, the aromatase activity in ovaries stimulated by 2.5 IU of PMS was suppressed by gestrinone and increased by MPA, and neither gestrinone nor MPA affected the production of aromatizable androgens from progesterone by these ovaries. Thus, gestrinone and MPA administrated in vivo showed divergent influences on steroidogenic enzyme activities in ovaries, but they did not affect the serum concentration of estradiol-17 beta. The present results suggest that neither gestrinone nor MPA reduced estrogen production by the rat ovary under the gonadotropin stimulation although they influenced some process of its steroidogenesis.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 6","pages":"615-21"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Generalized resistance to thyroid hormone (GRTH) in a family: case studies. 一个家庭对甲状腺激素(GRTH)的全身性抵抗:案例研究。

Endocrinologia japonica Pub Date : 1992-12-01 DOI: 10.1507/endocrj1954.39.533

K Tanaka, A Sugawara, M Sakamoto, T Inoue, A Yawata, O Koshimura, Y Sako, S Sasaki, A Shimatsu, H Nakamura

引用次数: 1

Effects of age, sex and renal function on urinary insulin-like growth factor I (IGF-I) levels in adults. 年龄、性别和肾功能对成人尿胰岛素样生长因子I (IGF-I)水平的影响

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.507

H Yamamoto, Y Kato

{"title":"Effects of age, sex and renal function on urinary insulin-like growth factor I (IGF-I) levels in adults.","authors":"H Yamamoto, Y Kato","doi":"10.1507/endocrj1954.39.507","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.507","url":null,"abstract":"Insulin-like growth factor I (IGF-I) levels in urine were measured in adults using specific RIA after extraction with acid-ammonium sulfate. Mean (+/- SD) total urine IGF-I values were 267.9 +/- 112.9 ng/day and 167.8 +/- 73.2 ng/g creatinine (Cr) in 17 normal young adults. There was a positive correlation (r = 0.785, P < 0.001) between IGF-I values in early morning urine and those of 24 h urine when they were corrected by urinary Cr. IGF-I values in early morning urine were ranged from 60 to 1,100 ng/gCr with a mean value of 309.6 ng/gCr in 178 normal adults aged 21-80 yr. There was a consistent trend towards higher urinary IGF-I values in males during aging and this trend did not reach statistical significance until the sixth and seventh decades. There was a positive correlation (r = 0.465, P < 0.005) between urinary IGF-I values and age in males but not in females. Although urinary IGF-I values were higher in females than in males of the second and third decades, no sex difference was found in older adults. Urinary IGF-I values were correlated reversely with 24 h Cr clearance (CCr) and positively with urinary beta 2-microglobulin (beta 2-MG) levels in patients with renal dysfunction. These findings indicate that urinary IGF-I levels are influenced by age, sex and renal function in adults.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"507-15"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Gonadotropin induces expression of c-fos and c-jun genes in rat ovaries. 促性腺激素诱导大鼠卵巢c-fos和c-jun基因的表达。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.455

H Shibata, N Suganuma, N Miyamoto, Y Murata, I Kondo, K Nakamura, Y Tomoda, N Matsui, H Seo

{"title":"Gonadotropin induces expression of c-fos and c-jun genes in rat ovaries.","authors":"H Shibata, N Suganuma, N Miyamoto, Y Murata, I Kondo, K Nakamura, Y Tomoda, N Matsui, H Seo","doi":"10.1507/endocrj1954.39.455","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.455","url":null,"abstract":"It has been shown that the expression of protooncogenes, c-fos and c-jun, induced by growth factors and hormones plays important roles in cellular proliferation, tissue differentiation and transcription of certain genes. Since gonadotropin stimulates ovarian steroidogenesis and cellular proliferation, we investigated whether gonadotropin affects the expression of c-fos and c-jun genes in rat ovaries. The expression of mRNA coding side chain cleavage enzyme (P450scc), the rate limiting enzyme in ovarian steroidogenesis was also studied. The effect of gonadotropin was examined in female rats whose gonadotrophs were medically ablated by GnRH agonist (TAP-144-SR). After intravenous administration of pregnant mare's serum gonadotropin (PMSG:30 IU/rat), their ovaries were dissected out at various time intervals and total RNA was extracted. Changes in the levels of c-fos, c-jun and P450scc mRNAs were determined by Northern blot analysis. The levels of c-fos and c-jun mRNAs increased rapidly and transiently with the peak levels at 15 min after PMSG administration. The levels of both mRNAs were decreased by 30 to 60 min. On the other hand, the levels of P450scc mRNA started to increase 60 min after PMSG. These results indicate that gonadotropin-induced increase in the expression of c-fos and c-jun genes may play important roles in mediating the action of gonadotropin on the ovaries.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"455-60"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.455","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12456871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Plasma free fatty acids, inhibitor of extrathyroidal conversion of T4 to T3 and thyroid hormone binding inhibitor in patients with various nonthyroidal illnesses. 各种非甲状腺疾病患者的血浆游离脂肪酸、甲状腺外T4转化为T3的抑制剂和甲状腺激素结合抑制剂。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.445

Y Suzuki, M Nanno, R Gemma, T Yoshimi

{"title":"Plasma free fatty acids, inhibitor of extrathyroidal conversion of T4 to T3 and thyroid hormone binding inhibitor in patients with various nonthyroidal illnesses.","authors":"Y Suzuki, M Nanno, R Gemma, T Yoshimi","doi":"10.1507/endocrj1954.39.445","DOIUrl":"10.1507/endocrj1954.39.445","url":null,"abstract":"In order to clarify the role of free fatty acid (FFA) in thyroid hormone abnormalities in patients with nonthyroidal illness, thyroid function, FFA, inhibitor of extrathyroidal conversion of T4 to T3 (IEC) and thyroid hormone binding inhibitor (THBI) were studied in 99 patients with various nonthyroidal illnesses including diabetes mellitus (DM) (n = 35), liver cirrhosis (LC) (n = 33), chronic obstructive pulmonary disease (COPD) (n = 17) and chronic heart failure (CHF) (n = 14). Patients were divided into three groups based on the level of serum T3: Group I (T3 < 50 ng/dl), Group II (50 < or = T3 < 80) and Group III (80 < or = T3). Serum T4, FT3 and the T3/T4 ratio decreased significantly in the order Group III, Group II and Group I (Group III > II > I). The plasma FFA level was 0.91 +/- 0.12 mmol/l in Group I (P < 0.05, vs. Group III), 0.65 +/- 0.06 in Group II and 0.54 +/- 0.04 in Group III, respectively. The incidence of positive IEC was 80.0% in Group I (P < 0.05, vs. Group III), 53.7% in Group II (P < 0.05, vs. Group III) and 34.2% in Group III. However, IEC was not correlated with the serum T3 concentration. The incidence of positive THBI was 80% in Group I (P < 0.05, vs. Group III), 68.3% in Group II and 47.4% in Group III, but THBI was not correlated with the serum T4 level. Positive correlations were observed among FFA, IEC and THBI (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"445-53"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Adrenalectomy enhances the susceptibility of pancreatic islets to interleukin-1 beta: immunohistochemical study. 肾上腺切除术增强胰岛对白细胞介素-1 β的敏感性:免疫组织化学研究。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.485

H Shimizu, S Tanaka, M Mori

引用次数: 2

Characterization of the insulin resistance in liver cirrhosis: a comparison with non-insulin dependent diabetes mellitus. 肝硬化胰岛素抵抗的特征:与非胰岛素依赖型糖尿病的比较。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.421

I Miyamoto, H Miyakoshi, Y Nagai, K Ohsawa, Y Nishimura, Y Noto, K Kobayashi

{"title":"Characterization of the insulin resistance in liver cirrhosis: a comparison with non-insulin dependent diabetes mellitus.","authors":"I Miyamoto, H Miyakoshi, Y Nagai, K Ohsawa, Y Nishimura, Y Noto, K Kobayashi","doi":"10.1507/endocrj1954.39.421","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.421","url":null,"abstract":"To characterize the mechanisms of insulin resistance in liver cirrhosis (LC), we estimated the peripheral tissue sensitivity and responsiveness to insulin using the euglycemic clamp technique and determined the insulin binding to erythrocytes in patients with compensated LC as well as in patients with non-insulin dependent diabetes mellitus (NIDDM). The insulin dose-response curves of the glucose metabolic clearance rates (MCR) were shifted to the right and downward both in patients with LC and NIDDM, indicating a reduced sensitivity and responsiveness to insulin. In the cirrhotics, MCR at the maximally effective insulin level, an index of insulin responsiveness, was correlated with fasting insulin levels (r = -0.57, P < 0.01) and sigma BG in 75 gOGTT (r = -0.43, P < 0.05), but no correlations were found between them and the diabetics. Although specific insulin bindings to erythrocytes were significantly lower in patients both with LC and NIDDM, Scatchard analysis revealed a significant decrease in the number of insulin receptors in the cirrhotics, and a decrease in the empty-site affinity in the diabetics. These findings suggest that insulin resistance in LC consists of a combination of binding and postbinding defects. The latter defect may be caused by basal hyperinsulinemia and contribute to the development of glucose intolerance. Although binding and postbinding abnormalities are also found in NIDDM, the mechanisms of insulin resistance in LC and NIDDM may be different.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"421-9"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.421","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12647185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An inhibitor of sweet taste response modulates glucose-induced phosphoinositide breakdown in rat pancreatic islets. 一种甜味反应抑制剂调节葡萄糖诱导的大鼠胰岛磷酸肌肽分解。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.439

K Tanigawa, M Furui, N Hara, M Sawada, Y Kato

{"title":"An inhibitor of sweet taste response modulates glucose-induced phosphoinositide breakdown in rat pancreatic islets.","authors":"K Tanigawa, M Furui, N Hara, M Sawada, Y Kato","doi":"10.1507/endocrj1954.39.439","DOIUrl":"https://doi.org/10.1507/endocrj1954.39.439","url":null,"abstract":"We studied the effect of a specific-competitive inhibitor of the sucrose taste response, p-nitrophenyl-D-glucopyranoside (PNP-Glu) on insulin release and phosphoinositide metabolism in rat pancreatic islets. The alpha-anomer, but not the beta-anomer, of PNP-Glu at a concentration of 5 mM inhibited insulin release induced by 10 mM glucose. Islets were labeled by exposure for 2 h to 10 uCi of myo-[2-3H] inositol solution supplemented with 2.8 mM glucose. Forty islets were then incubated in the presence of 10 mM LiCl, 1 mM inositol and 10 mM glucose with or without the anomers of PNP-Glu. [3H] radioactivity in the incubation medium remained significantly greater in the presence of the alpha-anomer of PNP-Glu than in the presence of glucose alone after 5- and 20-min incubation. The inositol monophosphate levels in the islets incubated with glucose alone were increased more than in the islets with alpha-anomer. The beta-anomer of PNP-Glu did not change either glucose-induced insulin release or phosphoinositide breakdown. A patch-clamp study revealed that neither anomer affected the glucose-dependent ATP-sensitive K(+)-channels. These results indicate that the anomeric preference for glucose in insulin release in the pancreatic islets is closely associated with phosphoinositide breakdown.","PeriodicalId":11534,"journal":{"name":"Endocrinologia japonica","volume":"39 5","pages":"439-44"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1507/endocrj1954.39.439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12508757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biochemical studies of the relationship between iodothyronine 5'-monodeiodinase and protein disulphide isomerase in rat liver. 大鼠肝脏碘甲状腺原氨酸5′-单脱碘酶与蛋白二硫异构酶关系的生化研究。

Endocrinologia japonica Pub Date : 1992-10-01 DOI: 10.1507/endocrj1954.39.461

S Sakane, I J Chopra, J Takamatsu, N Ohsawa

引用次数: 1