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Molecular Cloning, Genomic Organization, and Mapping of β4GalT-VIb, a brain Abundant Member of β4-galactosyltransferase Gene Family, to Human Chromosome 18q12.1 β4-半乳糖转移酶基因家族脑富集成员β4GalT-VIb在人类18q12.1染色体上的克隆、基因组组织及定位

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019838

Yuxin Fan, Long Yu, Q. Tu, Ruomu Gong, Ying Jiang, Qi Zhang, F. Dai, Chiyuan Chen, Shouyuan Zhao

{"title":"Molecular Cloning, Genomic Organization, and Mapping of β4GalT-VIb, a brain Abundant Member of β4-galactosyltransferase Gene Family, to Human Chromosome 18q12.1","authors":"Yuxin Fan, Long Yu, Q. Tu, Ruomu Gong, Ying Jiang, Qi Zhang, F. Dai, Chiyuan Chen, Shouyuan Zhao","doi":"10.1080/10425170290019838","DOIUrl":"https://doi.org/10.1080/10425170290019838","url":null,"abstract":"In the present study, a brain abundant member of g 4-galactosyltransferase gene family with an open reading frame encoding 343 amino acids was cloned and identified from a human leukemia cell cDNA library. The putative protein sequence is about 94.8 and 94.2% identical to the 382-amino-acid mouse and rat g 4-galactosyltransferase respectively and also contains cysteine residues previously shown to be important for the function of the gene family members. This cDNA (tentatively termed g 4GalT-VIb) is identical to a recently reported cDNA (tentatively termed g 4GalT-VIa) of human g 4-galactosyltransferase except lacking one exon, suggesting that these two cDNAs are two different alternative transcripts of the same gene. Northern hybridization showed that the new alternative transcript, g 4GalT-VIb, is expressed in all 16 human tissues tested with highest level in brain and rich level in testis, thymus and pancreas, whereas weak expression was detected in lung. The g 4GalT-VIb gene was located to human chromosome 18q12.1 between markers WI-9180 and SGC35630 by radiation hybrid mapping. The genomic organization and adjacent gene content of g 4GalT-VIb were identified by comparing its cDNA sequence with three genomic sequences AC017100, AP002474 and AP001336, which showed that g 4GalT-VIb spans an ~58 u kb region and is composed of 8 exons. In addition, the most conserved motif composed of 41 residues, LXYX 3 FGGVSXL(T/S)X 2 QFX 2 INGFPNX(Y/F)WGWGGEDDDX 2 NR, was defined according to 17 sequences of g 4GalTs from seven different organisms for the first time.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"57 1","pages":"1 - 8"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84064836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

cDNA Cloning and Molecular Characterization of Mink SMAD4 水貂SMAD4基因cDNA克隆及分子特性研究

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019892

Elizabeth Barnes, J. Askham, Pamela F. Jones

引用次数: 3

Identification of the Xenopus laevis cDNA for EXT1: A Phylogenetic Perspective 非洲爪蟾EXT1 cDNA的系统发育分析

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290029990

A. Hill, N. Brown, M. S. Hill, D. Wells

引用次数: 0

Isolation and Sequencing of a Putative Promoter Region of the Murine G Protein β1 Subunit ( GNB1 ) Gene 小鼠G蛋白β1亚基(GNB1)基因推定启动子区域的分离和测序

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019883

J. Kitanaka, N. Kitanaka, M. Takemura, Xiao‐Bing Wang, C. Hembree, N. L. Goodman, G. Uhl

{"title":"Isolation and Sequencing of a Putative Promoter Region of the Murine G Protein β1 Subunit ( GNB1 ) Gene","authors":"J. Kitanaka, N. Kitanaka, M. Takemura, Xiao‐Bing Wang, C. Hembree, N. L. Goodman, G. Uhl","doi":"10.1080/10425170290019883","DOIUrl":"https://doi.org/10.1080/10425170290019883","url":null,"abstract":"The expression of the heterotrimeric GTP-binding protein g 1 subunit gene ( GNB1 ) is regulated by psychostimulants such as cocaine and amphetamines. Since the up-regulation appears to be one of the candidate processes of sensitization, it is necessary to elucidate the cellular and molecular mechanism of the GNB1 gene regulation for a better understanding the establishment of sensitization. In the present study, we describe the isolation and nucleotide sequence analysis of the GNB1 gene promoter region. We have isolated approximately 10 u kb of the 5'-flanking region of the mouse of GNB1 gene and found potential elements involved in putative transcriptional control of the GNB1, such as AP1, AP2, Sp1, cyclic AMP response element, and nuclear factor s B recognition sites, within the sequences 0.3 u kb upstream from the putative transcription start site. This region was highly rich in G+C content, but lacked TATA or CATT boxes. Comparing the nucleotide sequence of the cDNA clone with the human genome databases using the blast program a region containing putative exon 1 and promoter of the human GNB1 gene in chromosome 1 was found. The cloning and sequence analysis of an extensive portion of the 5'-flanking regulatory region of the GNB1 gene provides new insights into the factors involved in the regulation by psychostimulants of GNB1 expression.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"39 1","pages":"39 - 45"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85039292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Nucleotide Sequences of the Internal Transcribed Spacers and 5.8S Region of Nuclear Ribosomal DNA In Pinus taeda L. and Pinus echinata Mill. 红松和紫松核核糖体DNA内部转录间隔区和5.8S区核苷酸序列。

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290030060

Jiwang Chen, C. Tauer, Yinghua Huang

引用次数: 6

Determination of Haploid DNA Sequences in Humans: Application to the Glucocerebrosidase Pseudogene 人类单倍体DNA序列的测定:葡萄糖脑苷酶假基因的应用

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019847

R. Martínez-Arias, J. Bertranpetit, D. Comas

引用次数: 9

Contamination in the Draft of the Human Genome Masquerades As Lateral Gene Transfer 人类基因组草案中的污染伪装成横向基因转移

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290023392

E. Willerslev, T. Mourier, A. Hansen, B. Christensen, I. Barnes, S. Salzberg

引用次数: 16

Molecular Analysis of an Endopolygalacturonase Gene from a Eucalyptus Canker Pathogen, Cryphonectria cubensis 一种桉树溃疡病病原多聚半乳糖醛酸酶基因的分子分析

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019874

P. Chimwamurombe, B. Wingfield, A. Botha, M. Wingfield

引用次数: 0

Phylogenetic Analysis of Hoxa 11 Sequences Reveals Absence of Transposable Elements, Conservation of Transcription Factor Binding Sites, and Suggests Antisense Coding Function Hoxa 11序列的系统发育分析揭示了转座元件的缺失，转录因子结合位点的保守，以及反义编码功能

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290029981

D. Bodenmiller, C. Baxter, David V. Hansen, S. Potter

{"title":"Phylogenetic Analysis of Hoxa 11 Sequences Reveals Absence of Transposable Elements, Conservation of Transcription Factor Binding Sites, and Suggests Antisense Coding Function","authors":"D. Bodenmiller, C. Baxter, David V. Hansen, S. Potter","doi":"10.1080/10425170290029981","DOIUrl":"https://doi.org/10.1080/10425170290029981","url":null,"abstract":"Nine thousand and eighty-eight base pairs of the chicken Hoxa 11 gene, including 8470 bases 5' of the translation start site were sequenced, and the characteristics of the upstream sequence investigated. Consistent with previous findings that middle repetitive elements are rare in the HoxA cluster, no repetitive elements were found other than simple oligonucleotide repeats. Multiple and pairwise alignments of the chicken upstream sequence with its human and mouse orthologs revealed multiple regions of 80% or higher homology across species. For the chicken, these regions were separated by sequences with no significant homology to human, mouse, or in most cases any other Genbank sequences. Selective clustering of transcription factor binding motifs was found to occur within the conserved homologous regions, suggesting evolutionary conservation of critical regulatory sequences. Of particular interest, seven conserved Cdx binding sites were found in the Hoxa 11 promoter, suggesting regulation by a non-clustered Caudal homeobox gene. Previous analysis of the mouse and human Hoxa 11 genes found a conserved antisense transcript, of unknown function. The chicken Hoxa 11 antisense strand included a conserved open reading frame capable of encoding 168 amino acids. Comparison of this region in mouse and chicken showed seven insertion/deletions, with each a multiple of three bases, thereby preserving open reading frame.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"17 1","pages":"77 - 83"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87409485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genomic Cloning of Xenopus TFIIS (TCEA1) and Identification of Its Transcription Start Site 爪蟾TFIIS (TCEA1)的基因组克隆及其转录起始位点的鉴定

DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019919

F. Kugawa, Masatada Aoki

引用次数: 2