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Complete Mitochondrial DNA Sequence and Amino Acid Analysis of the Cytochrome C Oxidase Subunit I ( COI ) from Aedes aegypti 埃及伊蚊细胞色素C氧化酶亚基I (COI)线粒体DNA全序列及氨基酸分析
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290030051
I. Morlais, D. Severson
{"title":"Complete Mitochondrial DNA Sequence and Amino Acid Analysis of the Cytochrome C Oxidase Subunit I ( COI ) from Aedes aegypti","authors":"I. Morlais, D. Severson","doi":"10.1080/10425170290030051","DOIUrl":"https://doi.org/10.1080/10425170290030051","url":null,"abstract":"The complete sequence of the yellow fever mosquito, Aedes aegypti, mitochondrial cytochrome c oxidase subunit 1 gene has been identified. The nucleotide sequence codes for a 512 amino acid peptide. The AeCOI sequence is A+T rich (68.6%) and the codon usage is highly biased toward a preference for A- or T-ending triplets. The A. aegypti COI peptide shows high homology, up to 93% identity, with several other insect sequences and a phylogenetic analysis indicates that the A. aegypti sequence is closely related to two other mosquito species, Anopheles gambiae and A. quadrimaculatus. Comparisons of the nucleotide sequence for four A. aegypti laboratory strains revealed single nucleotide polymorphisms, with 25 nucleotide sites showing SNPs between strains. All SNPs occurred as synonymous transitions such that the peptide sequence is conserved among A. aegypti strains. RT-PCR analysis showed that COI is expressed at similar levels in all developmental stages and tissues.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"32 1","pages":"123 - 127"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82824806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 50
Determination of Transcription Start Site and Analysis of Promoter Sequence, Splice Junction Sites, Intron Sequence and Codon Usage Bias of Rat Liver-specific Organic Anion Transporter-1 (rlst-1/Oatp-4/Slc21a10) Gene 大鼠肝脏特异性有机阴离子转运蛋白-1 (rlst-1/Oatp-4/Slc21a10)基因转录起始位点的确定、启动子序列、剪接连接位点、内含子序列和密码子使用偏好的分析
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290030015
S. Choudhuri, K. Ogura, C. Klaassen
{"title":"Determination of Transcription Start Site and Analysis of Promoter Sequence, Splice Junction Sites, Intron Sequence and Codon Usage Bias of Rat Liver-specific Organic Anion Transporter-1 (rlst-1/Oatp-4/Slc21a10) Gene","authors":"S. Choudhuri, K. Ogura, C. Klaassen","doi":"10.1080/10425170290030015","DOIUrl":"https://doi.org/10.1080/10425170290030015","url":null,"abstract":"The full-length coding sequence of rat liver specific organic anion transporter-1 (rlst-1/Oatp4/Slc21a10) was cloned by our group (Choudhuri et al., 2000) and by Cattori et al. (2000). We also cloned a splice variant of rlst-1 (Choudhuri et al., 2000). Another splice variant was cloned by Kakyo et al. (1999). We had also obtained a BAC clone from screening a BAC-Rat library, and determined the exon breakpoints to be able to determine the origin of the splice variants. Since our original publication, we have further characterized the rlst-1 gene. In this communication, we report the sequence of about 2.3 kb of the 50-flanking sequence of the rlst-1 gene that contains the promoter. We have mapped the transcription start site to define the precise location of the promoter. We also report here the splice junction sequence, and the partial sequences and lengths of introns of the rlst-1 gene. Finally, we have analyzed the codon usage bias in the rlst-1 coding sequence. Transcription start site was determined by capsite cloning as described by Choudhuri et al. (2001) with two major modifications. First, 20mg total RNA (instead of polyA mRNA) and secondly, Gene Racer RLM RACE system (In Vitrogen, CA), were used for this experiment. Sequence of the gene specific reverse primer (GSP), and nested gene specific reverse primer (nGSP) used, was as follows:","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"103 - 107"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76129186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Na - ctl -2, a cDNA Encoding a C-type Lectin Expressed Exclusively in Adult Necator americanus Hookworms 编码c型凝集素的cDNA Na - ctl -2在美洲钩虫成虫中的特异表达
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019900
A. Loukas, Alan P Brown, D. Pritchard
{"title":"Na - ctl -2, a cDNA Encoding a C-type Lectin Expressed Exclusively in Adult Necator americanus Hookworms","authors":"A. Loukas, Alan P Brown, D. Pritchard","doi":"10.1080/10425170290019900","DOIUrl":"https://doi.org/10.1080/10425170290019900","url":null,"abstract":"C-type lectins (C-TLs) are carbohydrate-binding proteins central to diverse physiological processes including immunity, venom-induced haemostasis and wound repair. Here we describe the cloning of Na - ctl -2, a cDNA encoding a secreted C-TL from the human hookworm Necator americanus. The transcript was detected in mRNA from adult worms but not infective larvae. The cDNA encoded an N-terminal secretory signal peptide followed by a long-form C-TL domain with sequence similarity to C-TL-like proteins from Caenorhabditis elegans and mammalian antigen presenting cell receptors, suggesting that hookworms might utilise this class of lectin to interrupt anti-parasite immune responses or interfere with host clotting mechanisms. This is the first report of a full-length cDNA encoding a lectin from hookworms. The unusually skewed representation of this protein family within different nematode genera and its subsequent impact on the evolution of nematode parasitism is discussed.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"42 1","pages":"61 - 65"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83900579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
A Major Substrate for MPF: cDNA Cloning and Expression of Polypeptide Chain Elongation Factor 1γ from Goldfish ( Carassius auratus ) MPF的主要底物:金鱼多肽链延伸因子1γ cDNA的克隆与表达
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019865
M. Tokumoto, Y. Nagahama, T. Tokumoto
{"title":"A Major Substrate for MPF: cDNA Cloning and Expression of Polypeptide Chain Elongation Factor 1γ from Goldfish ( Carassius auratus )","authors":"M. Tokumoto, Y. Nagahama, T. Tokumoto","doi":"10.1080/10425170290019865","DOIUrl":"https://doi.org/10.1080/10425170290019865","url":null,"abstract":"One of the eukaryotic polypeptide chain elongation factors, EF-1 g n i complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 f. In the complex, EF-1 n has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 n from the goldfish ovary. The cloned cDNA was 1490 u bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 n from other species. Although, the phosphorylation site identified in Xenopus EF-1 n was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 n was phosphorylated by MPF. We concluded that EF-1 n is a substrate for MPF during oocyte maturation in goldfish.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"27 - 31"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73937179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Cloning and Tissue-specific Expression of Spliced Variants of the Rat Organic Anion Transporter (rOAT-K) 大鼠有机阴离子转运蛋白(rOAT-K)剪接变体的克隆及组织特异性表达
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290019856
M. Wolff, T. Su
{"title":"Cloning and Tissue-specific Expression of Spliced Variants of the Rat Organic Anion Transporter (rOAT-K)","authors":"M. Wolff, T. Su","doi":"10.1080/10425170290019856","DOIUrl":"https://doi.org/10.1080/10425170290019856","url":null,"abstract":"During the last decade, molecular cloning has identified several families of multispecific organic ion transporters mediating the renal and hepatic elimination of organic ions. Clinically, these transporters play important roles in the renal tubular secretion and reabsorption of various drugs. They are also in part responsible for the drug pharmacologic responses, drug-drug interactions, and drug nephrotoxicity. This study describes 12 novel isoforms of the rat kidney organic anion transporter rOAT-K. These isoforms are spliced variants of the same gene arising from alternative splicing of six regions defined as A-F. The two previously reported isoforms rOAT-K1 and rOAT-K2 were also found to be spliced variants of this gene. The open reading frames of the 12 isoforms encode a range of 352-670 amino acid proteins. Tissue distribution studies showed that the majority of the isoforms are kidney- and liver-specific.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"16 1","pages":"15 - 25"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91288942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
cDNA Sequence of Porcine Thioredoxin 猪硫氧还蛋白cDNA序列
DNA Sequence Pub Date : 2002-01-01 DOI: 10.1080/10425170290030033
GraceHu Yu, Jiang-ying Xu, Lin Xu, Kin-Sing Lee
{"title":"cDNA Sequence of Porcine Thioredoxin","authors":"GraceHu Yu, Jiang-ying Xu, Lin Xu, Kin-Sing Lee","doi":"10.1080/10425170290030033","DOIUrl":"https://doi.org/10.1080/10425170290030033","url":null,"abstract":"Several mammalian thioredoxin cDNA sequences, namely that of human, macaca, ovine, bovine, equine and murine have been already registered in the Genbank database; but that of porcine is still not known. In this communication, we report the full-length cDNA sequence of porcine thioredoxin as determined by RT-PCR method. We also compared the protein sequence of thioredoxin from various mammals. Multialignment of the amino acid sequences between porcine and other mammalian species revealed that the sequences are highly conserved. Only one difference exists between the amino acid sequences of porcine and bovine thioredoxin.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"3 1","pages":"113 - 115"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87982679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
The Gas Vesicle Gene (gvp) Cluster of the Cyanobacterium Pseudanabaena sp. Strain PCC 6901 假藻蓝藻pcc6901的气体囊泡基因(gvp)簇
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084457
D. Albouy, A. Castets, N. T. de Marsac
{"title":"The Gas Vesicle Gene (gvp) Cluster of the Cyanobacterium Pseudanabaena sp. Strain PCC 6901","authors":"D. Albouy, A. Castets, N. T. de Marsac","doi":"10.3109/10425170109084457","DOIUrl":"https://doi.org/10.3109/10425170109084457","url":null,"abstract":"A gene cluster located downstream from gvpA in the cyanobacterium Pseudanabaena sp. strain PCC 6901 has been cloned and sequenced. The three genes, orfl, gvpN and gvpj, are consecutive with no intergenic region. In contrast to GvpN and Gvpj, which share high similarity at the amino acid level with their counterparts in other cyanobacteria and halophilic archaea, Orfl is only 29% identical to the C-terminal part of GvpC from Anabaena flos-aquae and its sequence organization is reminiscent of the halophilic archaeal GvpC.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"34 1","pages":"337 - 344"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78028141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Gene Structure and Alternative Splicing of Murine Podocalyxin: A member of the CD34 Sialomucin Family 小鼠Podocalyxin的基因结构和选择性剪接:CD34唾液蛋白家族的一员
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084466
Jian Li, Yong Li, P. Brophy, D. Kershaw
{"title":"Gene Structure and Alternative Splicing of Murine Podocalyxin: A member of the CD34 Sialomucin Family","authors":"Jian Li, Yong Li, P. Brophy, D. Kershaw","doi":"10.3109/10425170109084466","DOIUrl":"https://doi.org/10.3109/10425170109084466","url":null,"abstract":"Podocalyxin is a sialoglycoprotein of the glomerular podocytes, vascular endothelial cells, platelets, and hemopoietic stem cells. The function of podocalyxin is unknown, but it contains most of the protein bound sialic acid in the glomerulus and is considered vital in the structure and function of the glomerular filtration apparatus. The murine podocalyxin full-length cDNA has been determined and is 5318 base pairs. The gene localizes to chromosome 6B1 by FISH analysis and contains eight major exons with one additional alternatively spliced exon. The alternatively spliced isoform of podocalyxin codes for a truncated intracellular domain and is expressed in a tissue specific pattern in parallel with full-length podocalyxin. The organization of the gene structure of murine podocalyxin is similar to the murine CD34 gene and suggests a distant evolutionary relationship to CD34.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"56 1","pages":"407 - 412"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84923260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Analysis and Expression of algL, which Encodes Alginate Lyase in Pseudomonas Syringae Pv. Syringae 丁香假单胞菌海藻酸解酶基因algL的分析与表达。两
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084474
Lori A. Preston, C. Bender, N. Schiller
{"title":"Analysis and Expression of algL, which Encodes Alginate Lyase in Pseudomonas Syringae Pv. Syringae","authors":"Lori A. Preston, C. Bender, N. Schiller","doi":"10.3109/10425170109084474","DOIUrl":"https://doi.org/10.3109/10425170109084474","url":null,"abstract":"Pseudomonas syringae produces alginate, an exopo-lysaccharide that contributes to the virulence and epiphytic fitness of this phytopathogenic bacterium. P. syringae also produces the aZgL-encoded alginate lyase, which cleaves the alginate biopolymer via a β-elimination reaction. The algL gene from P. syringae maps to a 1134 bp region within the alginate biosynthetic operon, and is similar to algL from Halomonas marina, P. aeruginosa, Azotobacter chroococcum, and A. vinelandii. algL from P. syringae was over expressed in Escherichia coli; two periplas-mic forms of AlgL were overproduced (40 and 37kDa). Both forms were enzymatically active and recognized by antibodies raised against AlgL from P. aeruginosa. Analysis of the regions flanking algL revealed significant homology to algX and algl, genes previously identified in the biosynthetic operon of other alginate-producing bacteria.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"182 1","pages":"455 - 461"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80340978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Cloning and Sequence Analysis of cDNA for Heavy-chain Ferritin from the Canis familiaris 家犬重链铁蛋白cDNA的克隆与序列分析
DNA Sequence Pub Date : 2001-01-01 DOI: 10.3109/10425170109084465
D. Jeoung, H. Kim
{"title":"Cloning and Sequence Analysis of cDNA for Heavy-chain Ferritin from the Canis familiaris","authors":"D. Jeoung, H. Kim","doi":"10.3109/10425170109084465","DOIUrl":"https://doi.org/10.3109/10425170109084465","url":null,"abstract":"Ferritin serves as a storage protein for iron in animals. Complementary DNA encoding a heavy chain ferritin was cloned from the brain of Canis familiaris. The dog ferritin cDNA encodes a 182 amino acid that shows high levels of amino acid identity with vertebrate ferritins (90-98%). Near the cap region of the 5'-untranslated region, the dog H-ferritin mRNA displays a 28-nucleotide sequence that is exactly conserved in the corresponding region of the human and pig H-ferritin mRNA, thus making this sequence a prime candidate for involvement in the known translational regulation of H-ferritin by iron.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"47 1","pages":"401 - 406"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82152050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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