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引用次数: 15
摘要
真核生物多肽链延伸因子之一EF-1 g - n复合物通过EF-1 f的GDP/GTP交换活性参与多肽链延伸。在该复合物中,EF-1 n已被报道为成熟促进因子(MPF)的主要底物。本文报道了金鱼、鲫鱼、EF-1 n的克隆、测序和表达分析。克隆的cDNA全长1490 u bp,编码442个氨基酸。推导出的氨基酸序列与其他物种的EF-1 n高度同源。虽然在爪蟾EF-1 n中发现的磷酸化位点在金鱼同源物中并不保守,但磷酸化分析表明,金鱼EF-1 n被MPF磷酸化。我们认为EF-1 n是金鱼卵母细胞成熟过程中MPF的底物。
A Major Substrate for MPF: cDNA Cloning and Expression of Polypeptide Chain Elongation Factor 1γ from Goldfish ( Carassius auratus )
One of the eukaryotic polypeptide chain elongation factors, EF-1 g n i complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 f. In the complex, EF-1 n has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 n from the goldfish ovary. The cloned cDNA was 1490 u bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 n from other species. Although, the phosphorylation site identified in Xenopus EF-1 n was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 n was phosphorylated by MPF. We concluded that EF-1 n is a substrate for MPF during oocyte maturation in goldfish.