{"title":"水貂SMAD4基因cDNA克隆及分子特性研究","authors":"Elizabeth Barnes, J. Askham, Pamela F. Jones","doi":"10.1080/10425170290019892","DOIUrl":null,"url":null,"abstract":"The Smad family of proteins have been implicated as major components of the TGF g signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink ( Mustela vison ) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"52 1","pages":"47 - 53"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"cDNA Cloning and Molecular Characterization of Mink SMAD4\",\"authors\":\"Elizabeth Barnes, J. Askham, Pamela F. Jones\",\"doi\":\"10.1080/10425170290019892\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Smad family of proteins have been implicated as major components of the TGF g signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink ( Mustela vison ) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.\",\"PeriodicalId\":11381,\"journal\":{\"name\":\"DNA Sequence\",\"volume\":\"52 1\",\"pages\":\"47 - 53\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA Sequence\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10425170290019892\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170290019892","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
cDNA Cloning and Molecular Characterization of Mink SMAD4
The Smad family of proteins have been implicated as major components of the TGF g signalling pathway and are important mediators of its pleiotrophic effects. Here we describe the cloning and characterization of the mink ( Mustela vison ) ortholog of Smad4. Mink Smad4 has a high level of conservation to its human counterpart showing 96% homology at the DNA level and 99% at the amino acid level. This is in agreement with the close homologies seen for the rat and mouse orthologs. In vitro transcription and translation shows the expression of a protein of predicted molecular weight, of identical size to its human counterpart.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
