Drug Delivery and Translational Research最新文献

{"title":"Controlled co-delivery of anti-inflammatory drugs from bilayer polymer films coating a meniscus implant.","authors":"Alfonso F Blanco, Gustavo Lou, Alba Pensado-López, Aldo Ummarino, Fernando Torres Andón, José Crecente-Campo, María José Alonso","doi":"10.1007/s13346-025-01942-5","DOIUrl":"https://doi.org/10.1007/s13346-025-01942-5","url":null,"abstract":"<p><p>Knee osteoarthritis (OA), a degenerative joint disease, is increasingly prevalent worldwide and often results from a meniscal deterioration that leads to meniscus removal. Replacing the damaged meniscus with a non-biodegradable prosthesis offers an innovative solution to prevent OA progression, particularly in older patients. However, the long-term use of anti-inflammatory drugs for pain relief and prosthesis integration can cause severe off-target side effects. The objective of this work was to design and develop drug-loaded bilayer polymer films to be used as coatings for a meniscus polycarbonate urethane (PCU). The developed bilayer polymer films enabled a sustained release of two anti-inflammatory drugs - dexamethasone (DEX) and celecoxib (CLX) - with distinct release kinetics (1-4 weeks for DEX and 6-9 months for CLX). This release profile was defined to modulate post-surgical and chronic inflammation within the knee joint, respectively. Two bilayer prototypes showed consistent biodegradation, drug release, drug loading, and reproducibility. Furthermore, the systems were sterile, biocompatible, and maintained the anti-inflammatory efficacy of the released drugs, effectively reducing pro-inflammatory cytokine secretion from human primary macrophages.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D-loaded lipid nanoparticles: antioxidant properties, preparation, optimization, and in vitro characterization.","authors":"Khadeejeh Al-Smadi, Mohammad Imran, Ayyah Abdoh, David Liu, Khanh Phan, Newton Andreo Filho, Vania Rodrigues Leite-Silva, Yousuf Mohammed","doi":"10.1007/s13346-025-01946-1","DOIUrl":"https://doi.org/10.1007/s13346-025-01946-1","url":null,"abstract":"<p><p>Vitamin D₃-loaded lipid nanoparticles (Vit D₃-LNP), integrated into an azulene cream, were developed to enhance the topical delivery and stability of Vitamin D₃. The LNP was formulated using a lipid mixture and hot homogenization-ultrasonication, with comprehensive characterization revealing a particle size of 153.9 nm, a high zeta potential (-54.3 mV), and a PDI of 0.216, which TEM confirmed. Encapsulation efficiency was high (96.98%), indicating successful incorporation of Vitamin D₃ within the lipid matrix. Stability studies revealed the impact of light exposure on Vitamin D₃ degradation. In vitro, release, and skin penetration studies using Franz diffusion cells and two-photon microscopy demonstrated enhanced drug permeation and retention in deeper skin layers with the cream formulation. Cell Viability test confirmed high cell viability (~ 80-100%) for both free Vitamin D₃ and the LNP formulation; also inflammation test showed a significant reduction in ROS levels with Vitamin D₃-LNP treatment. These findings highlight the therapeutic value of LNP in managing conditions like Vitiligo, providing insights into the design of stable, effective Vitamin D₃ delivery systems for dermal applications, and offering a promising approach for advanced skin treatments.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical evaluation of saponin as a permeabilizer for anandamide encapsulation in extracellular vesicles.","authors":"Marie Auquière, Romano Terrasi, Viridiane Gratpain, Jessica Vanderstraeten, André Cronemberger-Andrade, Negar Mozaheb, Marie-Paule Mingeot-Leclercq, Anne des Rieux, Giulio G Muccioli","doi":"10.1007/s13346-025-01951-4","DOIUrl":"https://doi.org/10.1007/s13346-025-01951-4","url":null,"abstract":"<p><p>Extracellular Vesicles (EV) have received considerable attention as drug delivery systems. Research into the loading of various exogenous cargoes in EV has been expanding in recent years. While bioactive lipids are a class of highly interesting molecules, their loading in EV remains much less explored. Here, EV isolated from a microglial cell line were loaded with the endocannabinoid anandamide (AEA), using different approaches: passive incubation, sonication and permeabilization by saponin. The addition of saponin increased the amount of AEA detected in the EV suspension, as compared to passive incubation or sonication. However, our subsequent observations demonstrated that the higher quantity of AEA measured when using saponin was due to AEA solubilization into saponin micelles. The elimination of residual saponin differs according to the purification method used. Here, ultrafiltration (UF) did not eliminate saponin micelles, leading to a co-isolation of AEA-loaded EV and AEA-loaded micelles. In addition to AEA quantification by UPLC-MS/MS, we applied the generalized polarization (GP) value of Laurdan to study the impact of AEA incubation on the EV membrane. Our data show that AEA increased EV membrane fluidity, supporting AEA insertion into the membrane of the EV. More generally, this work raises awareness about the use of saponin as a permeabilizer for bioactive lipids encapsulation in EV.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asia Pacific Delivery Science Conference (APDSC) 2024 co-organised by the CRS Malaysia, Australia, Chinese Taipei and New Zealand Local Chapters.","authors":"Choon Fu Goh, Ju Yen Fu, Tushar Kumeria, Jui-Yang Lai, Yunching Chen","doi":"10.1007/s13346-025-01958-x","DOIUrl":"https://doi.org/10.1007/s13346-025-01958-x","url":null,"abstract":"","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-derived exosome-like nanovesicles: mechanisms and molecular understanding in neurological disorders with potential therapeutic applications.","authors":"Sevim Isik, Sedra Alhelwani, Aya Sahsahi, Hilal Balcilar, Bercem Yeman-Kiyak","doi":"10.1007/s13346-025-01955-0","DOIUrl":"https://doi.org/10.1007/s13346-025-01955-0","url":null,"abstract":"<p><p>Exosomes are nano vesicles secreted by the cells that play an essential role in intercellular communication, enabling the transport of bioactive molecules, including proteins, lipids, and nucleic acids. Among them, plant-derived exosome-like nanovesicles have attracted considerable interest due to their prospective therapeutic implications, especially for neurological disorders. This article provides an overview of the biogenesis of plant-derived exosome-like nanovesicles, compares their characteristics with mammalian-derived exosomes, and investigates their bioavailability and chemical composition. The article also discusses the mechanisms through which they are uptaken by cells, highlighting several cellular uptake pathways and their significance for targeted drug delivery. Moreover, it explains the molecular basis of neurological disorders and investigates how plant-derived exosome-like nanovesicles regulate intracellular signaling pathways, providing potential therapeutic benefits. Finally, it provides the latest advancements in engineering research, emphasizing biochemical modifications on the exosomal surface, loading therapeutic molecules into exosomes, and exosomes derived from genetically engineered plants, for more effective therapies in neurological disorders.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody-conjugated polymer nanoparticles for brain cancer.","authors":"San San Amelia Tai, Hooi Leong Loo, Athirah Bakhtiar, Paul Chi-Lui Ho, Lay Hong Chuah","doi":"10.1007/s13346-025-01947-0","DOIUrl":"https://doi.org/10.1007/s13346-025-01947-0","url":null,"abstract":"","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric nanoparticle-mediated GBA1 gene therapy is neuroprotective in a preclinical model of Parkinson's disease.","authors":"Mohit Kwatra, Gijung Kwak, Haolin Li, Jung Soo Suk, Han Seok Ko","doi":"10.1007/s13346-025-01944-3","DOIUrl":"10.1007/s13346-025-01944-3","url":null,"abstract":"","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved cognition and memory via PLGA nanoparticle-mediated delivery of curcumin and piperine in an in vivo Alzheimer's disease model.","authors":"Zhi Xin Phuna, Shantini Vijayabalan, Bibhu Prasad Panda, Naveen Kumar Hawala Shivashekaregowda, Mohd Farooq Shaikh, Priya Madhavan","doi":"10.1007/s13346-025-01945-2","DOIUrl":"https://doi.org/10.1007/s13346-025-01945-2","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a multifactorial neurodegenerative disease that causes dementia, impaired cognitive function, and disorientation. Studies have revealed that curcumin and piperine were found to be neuroprotective for patients with dementia. Nevertheless, both compounds are known for their poor solubility. To address issues related to poor bioavailability, polymeric nanoparticles loaded with curcumin and piperine, in combination, were fabricated and characterized through physicochemical, surface morphology, drug-excipient compatibility, and bioavailability studies. The nanoparticle with the highest bioavailability was selected for pharmacokinetics and pharmacodynamics studies. Optimized Poly (D, L-lactide-co-glycolide) nanoparticles loaded with curcumin and piperine, which we refer to as functional nanoparticles (FNP), were successfully developed using the emulsion diffusion-high-pressure homogenization-solvent evaporation (EHS) technique with Poloxamer 188 as the stabilizer. Among nine formulations obtained, FNP1 had a particle size of 116.6 ± 2.13 nm and a zeta potential of -27.9 ± 1.51 mV. Saturation solubility and in vitro drug release studies demonstrated an enhanced solubility of curcumin and piperine in FNP1 compared to the pure compounds. Oral administration of FNP1 in a streptozotocin (STZ)-induced AD rat model resulted in significant improvement of spatial memory, as demonstrated by both the Morris Water Maze and Passive Avoidance tests. Further histology studies, which involved staining the cortex and hippocampus regions, revealed a significantly reduced number of pyramidal cells with extensive nuclear pyknosis and degeneration, a finding previously observed in untreated STZ-induced AD rats. This study concluded that the polymeric nanoparticle developed, FNP1, had successfully improved the solubility and bioavailability of curcumin and piperine, thereby enhancing cognitive and memory impairment in STZ-induced AD rats.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144946802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined anti-inflammatory and laxative effect of mango kernels-based effervescent suppositories loaded with (multi)metallic nanoparticles synthesized using ash of plantain peels and gel of Aloe vera.","authors":"Joseph Kyana, Régule S Botha, Bernard B Lufua, Christian O Lotanga, Enosch O Malutshi, Arlain L Mundeke, Vinel M Eyobi, Espoir K Kambale, Yannick B Nuapia, Aistė Balčiūnaitienė, Patrick B Memvanga","doi":"10.1007/s13346-025-01959-w","DOIUrl":"10.1007/s13346-025-01959-w","url":null,"abstract":"<p><p>The prolonged use of conventional steroidal and non-steroidal anti-inflammatory drugs, as well as laxatives, often leads to severe adverse effects and toxicity, posing significant health risks. To address these challenges, we formulated a novel therapy based on (multi)metallic nanoparticles (MMNPs) green-synthesized from agricultural by-products, namely plantain peels (Musa paradisiaca) and Aloe vera gel. The MMNPs were characterized through UV-visible spectrophotometry, energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS), revealing nanoparticle sizes between 250 and 400 nm. These MMNPs were incorporated into effervescent suppositories using Mangifera indica almond butter as a base to facilitate rectal administration and induce laxative effects in situ. The anti-inflammatory and laxative properties of the MMNPs were evaluated in guinea pigs (Cavia porcellus) used as animal model, in which inflammation and constipation were induced by formaldehyde and loperamide, respectively. Remarkably, at 60 min, the effervescent suppositories loaded with MMNPs and MMNPs enriched with ash demonstrated inhibition rates of 74.13% and 69.39%, respectively, for paw edema, outperforming the reference drug indomethacin, which showed an inhibition rate of 73.27%. Furthermore, the effervescent suppositories effectively triggered a laxative response, combating constipation with notable efficiency. This study demonstrates the potential of green-synthesized MMNPs derived from Musa paradisiaca peels and Aloe vera gel as a promising, eco-friendly therapeutic alternative for managing both inflammation and constipation, minimizing the risk of side effects associated with conventional treatments.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-assembling RADA16-I peptide in situ hydrogel loaded with Celastrol boost immunogenic cell death in oral squamous cell carcinoma.","authors":"Chanjuan Zhang, Jiajun Wu, Hongfang Li, Yaning Shi, Yantian Liang, Jingxin Chen, Li Qin","doi":"10.1007/s13346-025-01938-1","DOIUrl":"https://doi.org/10.1007/s13346-025-01938-1","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors of the head and neck and is characterized by high heterogeneity and high recurrence rates. In particular, the serious side effects of radiotherapy and chemotherapy are the main obstacles in OSCC treatment. It is worth noting that immune infiltration is associated with the occurrence of OSCC. However, the effective induction of a robust immune response remains challenging because of the limited responsiveness of most patients with oral cancer. Celastrol (CeT) has excellent therapeutic efficacy against cancers, but is poorly soluble in water. We used RADA16-I hydrogel loaded with CeT (RADA-CeT) to improve its solubility and stability. The results indicated that the complex formed by the interaction between RADA16-I and CeT exhibited better stability, smaller particle size, excellent dispersibility, and high elasticity. Particularly, RADA-CeT hydrogel was more effective in activating damage-associated molecular patterns, thereby evoking immunogenic cell death (ICD) in OSCC cells. In vivo experiments demonstrated that RADA-CeT hydrogel had a stronger promoting effect on the expressions of CD4, CD8, calreticulin (CRT), and high-mobility group box-1 (HMGB1). Thus, RADA-CeT exhibited excellent anti-tumor efficacy by amplifying ICD. In the present study, we developed a biocompatible drug delivery system for uncaging the power of CeT in OSCC immunotherapy.</p>","PeriodicalId":11357,"journal":{"name":"Drug Delivery and Translational Research","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144871941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}