Developmental Neurobiology最新文献_第5页

Large-scale waves of activity in the neonatal mouse brain in vivo occur almost exclusively during sleep cycles 活体新生小鼠大脑的大规模活动波几乎只发生在睡眠周期

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-10-17 DOI: 10.1002/dneu.22901

Dennis R. Tabuena, Randy Huynh, Jenna Metcalf, Thomas Richner, Albrecht Stroh, Bingni W. Brunton, William J. Moody, Curtis R. Easton

{"title":"Large-scale waves of activity in the neonatal mouse brain in vivo occur almost exclusively during sleep cycles","authors":"Dennis R. Tabuena, Randy Huynh, Jenna Metcalf, Thomas Richner, Albrecht Stroh, Bingni W. Brunton, William J. Moody, Curtis R. Easton","doi":"10.1002/dneu.22901","DOIUrl":"10.1002/dneu.22901","url":null,"abstract":"Spontaneous electrical activity plays major roles in the development of cortical circuitry. This activity can occur highly localized regions or can propagate over the entire cortex. Both types of activity coexist during early development. To investigate how different forms of spontaneous activity might be temporally segregated, we used wide-field trans-cranial calcium imaging over an entire hemisphere in P1–P8 mouse pups. We found that spontaneous waves of activity that propagate to cover the majority of the cortex (large-scale waves; LSWs) are generated at the end of the first postnatal week, along with several other forms of more localized activity. We further found that LSWs are segregated into sleep cycles. In contrast, cortical activity during wake states is more spatially restricted and the few large-scale forms of activity that occur during wake can be distinguished from LSWs in sleep based on their initiation in the motor cortex and their correlation with body movements. This change in functional cortical circuitry to a state that is permissive for large-scale activity may temporally segregate different forms of activity during critical stages when activity-dependent circuit development occurs over many spatial scales. Our data also suggest that LSWs in early development may be a functional precursor to slow sleep waves in the adult, which play critical roles in memory consolidation and synaptic rescaling.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 7-8","pages":"596-612"},"PeriodicalIF":3.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9419421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sex differences in myelination of the zebra finch vocal control system emerge relatively late in development 斑胸草雀声音控制系统髓鞘形成的性别差异在发育中相对较晚出现

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-10-07 DOI: 10.1002/dneu.22900

Adriana Diez, Shenghan Wang, Nicole Carfagnini, Scott A. MacDougall-Shackleton

{"title":"Sex differences in myelination of the zebra finch vocal control system emerge relatively late in development","authors":"Adriana Diez, Shenghan Wang, Nicole Carfagnini, Scott A. MacDougall-Shackleton","doi":"10.1002/dneu.22900","DOIUrl":"10.1002/dneu.22900","url":null,"abstract":"The role of myelination in the development of motor control is widely known, but its role in the development of cognitive abilities is less understood. Here, we examined sex differences in the development of myelination of structures and tracts that support song learning and production in songbirds. We collected brains from 63 young male and female zebra finches (Taeniopygia guttata) over four stages of development that correspond to different stages of song learning. Using a myelination marker (myelin basic protein), we measured the development of myelination in three different nuclei of the vocal control system (HVC, RA, and lateral magnocellular nucleus of the anterior nidopallium [LMAN]) and two tracts (HVC-RA and lamina mesopallium ventralis [LMV]). We found that the myelination of the vocal control nuclei and tracts is sex related and male biased. In males, the patterns of myelination were age-dependent, asynchronous in rate and progression and associated with the development of song learning and production. In females, myelination of vocal control nuclei was low or absent and did not significantly change with age. Sex differences in myelination of the HVC-RA tract were large and emerged late in development well after sex differences in the size of vocal control brain regions are established. Myelination of this tract in males coincides with the age of song crystallization. Overall, the changes in myelination in the vocal control areas and tracts measured are region-, age-, and sex-specific and are consistent with sex differences in song development.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 7-8","pages":"581-595"},"PeriodicalIF":3.0,"publicationDate":"2022-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cortical hem lacks stem cell potential despite expressing SOX9 and HOPX 尽管皮质边缘表达SOX9和HOPX，但缺乏干细胞潜能

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-09-06 DOI: 10.1002/dneu.22899

Alessia Caramello, Christophe Galichet, Miriam Llorian Sopena, Robin Lovell-Badge, Karine Rizzoti

{"title":"The cortical hem lacks stem cell potential despite expressing SOX9 and HOPX","authors":"Alessia Caramello, Christophe Galichet, Miriam Llorian Sopena, Robin Lovell-Badge, Karine Rizzoti","doi":"10.1002/dneu.22899","DOIUrl":"10.1002/dneu.22899","url":null,"abstract":"The adult dentate gyrus (DG) of rodents hosts a neural stem cell (NSC) niche capable of generating new neurons throughout life. The embryonic origin and molecular mechanisms underlying formation of DG NSCs are still being investigated. We performed a bulk transcriptomic analysis on mouse developing archicortex conditionally deleted for Sox9, a SoxE transcription factor controlling both gliogenesis and NSC formation, and identified Hopx, a recently identified marker of both prospective adult DG NSCs and astrocytic progenitors, as being downregulated. We confirm SOX9 is required for HOPX expression in the embryonic archicortex. In particular, we found that both NSC markers are highly expressed in the cortical hem (CH), while only weakly in the adjacent dentate neuroepithelium (DNE), suggesting a potential CH embryonic origin for DG NSCs. However, we demonstrate both in vitro and in vivo that the embryonic CH, as well as its adult derivatives, lacks stem cell potential. Instead, deletion of Sox9 in the DNE affects both HOPX expression and NSC formation in the adult DG. We conclude that HOPX expression in the CH is involved in astrocytic differentiation downstream of SOX9, which we previously showed regulates DG development by inducing formation of a CH-derived astrocytic scaffold. Altogether, these results suggest that both proteins work in a dose-dependent manner to drive either astrocytic differentiation in CH or NSC formation in DNE.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 7-8","pages":"565-580"},"PeriodicalIF":3.0,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10628860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enriched environment rescues neonatal pain induced cognitive deficits and the impaired hippocampal synaptic plasticity later in life 丰富的环境挽救新生儿疼痛引起的认知缺陷和海马突触可塑性受损

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-08-09 DOI: 10.1002/dneu.22898

Cuiting Min, Ru Ling, Mengying Chen, Dongqing Xia, Ran Chen, Xiaonan Li

{"title":"Enriched environment rescues neonatal pain induced cognitive deficits and the impaired hippocampal synaptic plasticity later in life","authors":"Cuiting Min, Ru Ling, Mengying Chen, Dongqing Xia, Ran Chen, Xiaonan Li","doi":"10.1002/dneu.22898","DOIUrl":"10.1002/dneu.22898","url":null,"abstract":"Although extensive and untreated pain that occurs during a critical developmental window may impair cognition later in life, environmental interventions early in life might promote cognition. However, the underlying mechanism is poorly understood. Our current study utilized a rat model of “repetitive needle pricks” from the day of birth (P0) to postnatal day 7 (P7) to mimic the painful experience of preterm neonates in the neonatal intensive care unit. Enriched environment (EE) during development period (from P15 to P70) was implemented as a nonpharmacological intervention approach. Electrophysiological recording, behavioral tests, and biochemical analysis were performed after the end of EE (between P71 and P80). The results showed neonatal repetitive pain resulted in a reduction in mechanical withdrawal thresholds by the von Frey test in P70 (p < .001). Furthermore, neonatal repetitive pain impaired spatial learning and memory (p < .05) and even led to dysfunction in fear memory (p < .01). In contrast, EE rescued neonatal pain-induced cognitive deficits and normalized hippocampal long-term potentiation in rats exposed to neonatal pain (p << .05). The beneficial effect of EE might be the improvements in hippocampal synaptic plasticity via upregulating neurotrophic factors and N-methyl-d-aspartate (NMDA) receptors in the hippocampus. Our findings provide evidence that early environmental intervention might be a safe strategy to overcome neurodevelopmental abnormalities in preterm infants who experienced multiple procedural painful events during the early critical period.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 6","pages":"545-561"},"PeriodicalIF":3.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40597616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Corticogenesis across species at single-cell resolution 单细胞分辨率下不同物种间的皮质发生

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-08-06 DOI: 10.1002/dneu.22896

Seon Hye E. Park, Ana K. Ortiz, Genevieve Konopka

{"title":"Corticogenesis across species at single-cell resolution","authors":"Seon Hye E. Park, Ana K. Ortiz, Genevieve Konopka","doi":"10.1002/dneu.22896","DOIUrl":"10.1002/dneu.22896","url":null,"abstract":"The neocortex (or pallium) consists of diverse cell types that are organized in a highly species-specific manner under strict spatiotemporal control during development. Many of the cell types are present transiently throughout development but contribute to permanent species-specific cortical features that are acquired through evolution. Therefore, capturing cell type-specific biological information has always been an important quest in the field of neurodevelopment. The progress in achieving fine cellular resolution has been slow due to technical challenges. However, with recent advancements in single-cell and multi-omics technologies, many laboratories have begun to successfully interrogate cellular and molecular mechanisms driving corticogenesis at single-cell resolution. In this review, we provide summarized results from many primary publications and several in-depth review articles that utilize or address single-cell genomics techniques to understand important topics, such as cellular and molecular mechanisms governing cortical progenitor proliferation, cell lineage progression, neuronal specification, and arealization, across multiple gyrencephalic (i.e., human and non-human primates) and lissencephalic species (i.e., mouse, reptiles, and songbirds). We also examine findings from recent studies involving epigenomic and posttranscriptional regulation of corticogenesis. In the discussion section, we provide our insights on the challenges the field currently faces as well as promising future applications of single cell technologies.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 6","pages":"517-532"},"PeriodicalIF":3.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10506083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

CRMP2 and CRMP4 are required for the formation of commissural tracts in the developing zebrafish forebrain CRMP2和CRMP4是发育中的斑马鱼前脑联合束形成所必需的

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-08-05 DOI: 10.1002/dneu.22897

Youjia Guo, Carolina Fiallos Oliveros, Toshio Ohshima

{"title":"CRMP2 and CRMP4 are required for the formation of commissural tracts in the developing zebrafish forebrain","authors":"Youjia Guo, Carolina Fiallos Oliveros, Toshio Ohshima","doi":"10.1002/dneu.22897","DOIUrl":"10.1002/dneu.22897","url":null,"abstract":"Axonal connections between the two sides of the brain are essential for processing sensorimotor functions, especially in animals with bilateral symmetry. The anterior commissure and postoptic commissure are two crucial axonal projections that develop early in the zebrafish central nervous system. In this study, we characterized the function of collapsin response mediator protein 2 (CRMP2) and CRMP4 in patterning the development of the anterior and postoptic commissures by analyzing morpholino-knockdown zebrafish morphants and CRISPR/Cas9-edited gene-knockout mutants. We observed a loss of commissural structures or a significant reduction in axon bundles connecting the two hemispheres, but the defects could be largely recovered by co-injecting CRMP2 or CRMP4 mRNA. Loss of both CRMP2 and CRMP4 function resulted in a synergistic increase in the number of commissural defects. To elucidate the mechanism by which CRMP2 and CRMP4 provide guidance cues for the development of the anterior and postoptic commissures, we included neuropilin 1a (Nrp1a) morphants and double morphants (CRMP2/Nrp1a and CRMP4/Nrp1a) for analysis. Our experimental results indicated that CRMP2 and CRMP4 might mediate their activities through the common semaphorin 3/Nrp1a signaling pathway.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 6","pages":"533-544"},"PeriodicalIF":3.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40585750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dachshund acts with Abdominal-B to trigger programmed cell death in the Drosophila central nervous system at the frontiers of Abd-B expression Dachshund与腹腔b在果蝇中枢神经系统Abd-B表达的前沿触发程序性细胞死亡

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-07-07 DOI: 10.1002/dneu.22894

Luis Clarembaux-Badell, Pablo Baladrón-de-Juan, Hugo Gabilondo, Irene Rubio-Ferrera, Irene Millán, Carlos Estella, Félix S. Valverde-Ortega, Ignacio Monedero Cobeta, Stefan Thor, Jonathan Benito-Sipos

{"title":"Dachshund acts with Abdominal-B to trigger programmed cell death in the Drosophila central nervous system at the frontiers of Abd-B expression","authors":"Luis Clarembaux-Badell, Pablo Baladrón-de-Juan, Hugo Gabilondo, Irene Rubio-Ferrera, Irene Millán, Carlos Estella, Félix S. Valverde-Ortega, Ignacio Monedero Cobeta, Stefan Thor, Jonathan Benito-Sipos","doi":"10.1002/dneu.22894","DOIUrl":"10.1002/dneu.22894","url":null,"abstract":"A striking feature of the nervous system pertains to the appearance of different neural cell subtypes at different axial levels. Studies in the Drosophila central nervous system reveal that one mechanism underlying such segmental differences pertains to the segment‐specific removal of cells by programmed cell death (PCD). One group of genes involved in segment‐specific PCD is the Hox homeotic genes. However, while segment‐specific PCD is highly precise, Hox gene expression is evident in gradients, raising the issue of how the Hox gene function is precisely gated to trigger PCD in specific segments at the outer limits of Hox expression. The Drosophila Va neurons are initially generated in all nerve cord segments but removed by PCD in posterior segments. Va PCD is triggered by the posteriorly expressed Hox gene Abdominal‐B (Abd‐B). However, Va PCD is highly reproducible despite exceedingly weak Abd‐B expression in the anterior frontiers of its expression. Here, we found that the transcriptional cofactor Dachshund supports Abd‐B‐mediated PCD in its anterior domain. In vivo bimolecular fluorescence complementation analysis lends support to the idea that the Dachshund/Abd‐B interplay may involve physical interactions. These findings provide an example of how combinatorial codes of transcription factors ensure precision in Hox‐mediated PCD in specific segments at the outer limits of Hox expression.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 6","pages":"495-504"},"PeriodicalIF":3.0,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ac/92/DNEU-82-495.PMC9544350.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40590665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Modulation of calcineurin signaling during development 发育过程中钙调磷酸酶信号的调节

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-07-03 DOI: 10.1002/dneu.22895

Sara Tucker Edmister, Robbert Creton

{"title":"Modulation of calcineurin signaling during development","authors":"Sara Tucker Edmister, Robbert Creton","doi":"10.1002/dneu.22895","DOIUrl":"10.1002/dneu.22895","url":null,"abstract":"Calcineurin signaling pathways are suppressed in Down syndrome (trisomy 21), by overexpression of genes that are located on chromosome 21. Two key genes are the regulator of calcineurin 1 (RCAN1), also called the Down syndrome critical region 1 (DSCR1), and the dual-specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A). The suppressed calcineurin pathway may potentially be restored using small-molecule DYRK inhibitors, which have been proposed as therapeutics in Down syndrome. However, little is known about the benefits and risks of such treatments during various stages of embryonic development, fetal development, and childhood. We examined the modulation of calcineurin signaling during development, using zebrafish as a model system. To mimic suppressed calcineurin signaling in Down syndrome, zebrafish were exposed to the calcineurin inhibitors cyclosporine and tacrolimus during development. We found that suppression of calcineurin signaling changed specific larval behaviors, including activity and responses to acoustic and visual stimuli, depending on the period of exposure. Cotreatment with the DYRK inhibitor proINDY restored a few of these behaviors but also induced a range of adverse side effects including decreased activity and reduced optomotor responses to visual stimuli. Based on these results, we conclude that proINDY has limited benefits and substantial risks when used during development. We propose that zebrafish is an efficient model system for preliminary safety and efficacy tests of other DYRK inhibitors that aim to restore calcineurin signaling during neural development.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"82 6","pages":"505-516"},"PeriodicalIF":3.0,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10131871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information 问题信息

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-07-01 DOI: 10.1002/ocea.5298

引用次数: 0

Evolutionary innovations of human cerebral cortex viewed through the lens of high-throughput sequencing 通过高通量测序观察人类大脑皮层的进化创新

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-06-28 DOI: 10.1002/dneu.22893

Ikuo K. Suzuki

引用次数: 0