Developmental Neurobiology最新文献

Prolactin Secretion During Postnatal Development in Artificial Rearing Rats (Rattus norvegicus) 人工饲养大鼠（褐家鼠）产后发育过程中催乳素分泌的研究

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-23 DOI: 10.1002/dneu.22985

César G. Toriz, Ángel I. Melo, Brenda B. García-Iglesias, Carmen Solano-Agama, María de los Ángeles Martínez-Muñoz, Elsa Liliana Aguirre-Benítez, Verónica Rodríguez-Mata, Dulce Ávila-Rodríguez, Margarita González-del-Pliego, Armando Pérez-Torres, Ismael Jiménez-Estrada, Benjamín Floran-Garduño, María Eugenia Mendoza-Garrido

{"title":"Prolactin Secretion During Postnatal Development in Artificial Rearing Rats (Rattus norvegicus)","authors":"César G. Toriz, Ángel I. Melo, Brenda B. García-Iglesias, Carmen Solano-Agama, María de los Ángeles Martínez-Muñoz, Elsa Liliana Aguirre-Benítez, Verónica Rodríguez-Mata, Dulce Ávila-Rodríguez, Margarita González-del-Pliego, Armando Pérez-Torres, Ismael Jiménez-Estrada, Benjamín Floran-Garduño, María Eugenia Mendoza-Garrido","doi":"10.1002/dneu.22985","DOIUrl":"https://doi.org/10.1002/dneu.22985","url":null,"abstract":"<p>During the lactation period, rat pups are fed by their mother and are with their siblings. In the pituitary, lactotrope and somatolactotrope cells increase in number during this period and are associated with milk-borne factors. In the artificial rearing (AR) paradigm, pups are deprived of mother– and sibling–pup interactions and are fed artificial milk. AR rats present more hypothalamic neurons associated with less apoptosis during postnatal development. Moreover, AR infantile rats show growth hormone (GH), insulin-like growth factor-1 (IGF-1), and ghrelin alterations resulting from suckling behavior and the absence of meal transition at the second week of life. In the present study, the variation in prolactin (PRL) throughout the lactation period was analyzed in AR pups and compared with that in mother-reared (MR) pups. At postnatal Day 7 (pnd7), AR pups have less serum PRL than MR pups do, and a further decrease was observed at pnd14. However, only at pnd14, AR pituitary cells secrete less PRL, which was correlated with a smaller number of somatolactotrope cells unlike lactotrope cells. Analysis of the hypothalamic dopamine and DOPAC concentrations in both groups revealed no differences at 7, 14, and 21pnd. Nevertheless, the pituitary showed higher concentrations in AR pups than in MR pups at pnd14. However, the number of dopaminergic neurons in the arcuate nucleus was similar in both groups, but they were less spread in the AR pup hypothalamus. Our results revealed the importance of mothers’ and siblings’ interactions and mothers’ milk in the maturation of the PRL hypothalamic‒pituitary axis during the lactating period.</p>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust Brain Tumor Detection and Classification From Multichannel MRI Using Deep Learning 基于深度学习的多通道MRI稳健脑肿瘤检测与分类

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-21 DOI: 10.1002/dneu.22991

Prasad A. Y., Kazuaki Tanaka, Krishnamoorthy R., R. Thiagarajan

{"title":"Robust Brain Tumor Detection and Classification From Multichannel MRI Using Deep Learning","authors":"Prasad A. Y., Kazuaki Tanaka, Krishnamoorthy R., R. Thiagarajan","doi":"10.1002/dneu.22991","DOIUrl":"https://doi.org/10.1002/dneu.22991","url":null,"abstract":"<div>\u0000 \u0000 <p>Brain tumor detection and classification from multichannel magnetic resonance imaging (MRI) using deep learning techniques for an accurate detection and classification of brain tumors from multichannel MRI are essential for guiding effective treatment strategies and improving patient outcomes. Traditional methods often struggle with handling large volumes of MRI data, leading to limitations in both efficiency and reliability. This study aims to develop a robust approach for brain tumor detection and classification by leveraging computer vision and deep learning techniques, addressing the limitations of conventional methods. The proposed approach utilizes the dual boundary-sensitive transformation (DBST) algorithm for precise tumor edge detection, whereas the scale-invariant feature transform (SIFT) method provides robust and invariant features for classification. Additionally, deep learning models, DarkNet53 and DenseNet201, are employed to enhance classification performance by learning complex patterns from a large dataset of multichannel MRI images. The dataset used in this study is publicly available, ensuring reproducibility and accessibility of the research. The results show a specificity of 98%, indicating the model's strong ability to correctly identify negative cases, and a sensitivity of 99%, demonstrating its effectiveness in identifying positive cases. This performance significantly surpasses traditional methods and is competitive with state-of-the-art (SOTA) techniques in the field. MATLAB is utilized to implement the models, showcasing the potential of deep learning in medical imaging. Future work will explore more advanced deep learning architectures, incorporate additional modalities, and further refine the techniques to improve accuracy and robustness in brain tumor detection and classification.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Vitamin A Deficiency and Supplementation on Behavioral and Oxidative Stress Markers in Male Offspring of a Valproic Acid-Induced Autism Rat Model 维生素A缺乏和补充对丙戊酸诱导的自闭症大鼠模型雄性后代行为和氧化应激标志物的影响

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-07 DOI: 10.1002/dneu.22990

Kacimi Fatima Ezzahra, Ed-Day Soumia, Nechchadi Habiba, Ibouzineddine Laila, Ramchoun Mhamed, Berrougui Hicham, Azzaoui Fatima-Zahra, Boulbaroud Samira

{"title":"The Impact of Vitamin A Deficiency and Supplementation on Behavioral and Oxidative Stress Markers in Male Offspring of a Valproic Acid-Induced Autism Rat Model","authors":"Kacimi Fatima Ezzahra, Ed-Day Soumia, Nechchadi Habiba, Ibouzineddine Laila, Ramchoun Mhamed, Berrougui Hicham, Azzaoui Fatima-Zahra, Boulbaroud Samira","doi":"10.1002/dneu.22990","DOIUrl":"https://doi.org/10.1002/dneu.22990","url":null,"abstract":"<div>\u0000 \u0000 <p>Autism spectrum disorder (ASD) is a neurodevelopmental condition influenced by genetic and environmental factors, including prenatal nutrition. This study investigates the role of Vitamin A deficiency (VAD) in exacerbating ASD-like behaviors in a valproic acid (VPA)-induced rat model and mitigating effects of Vitamin A supplementation (VAS). Twenty-five pregnant rats were divided into five groups, with treatments including VPA exposure and VAD. Neurodevelopmental and behavioral tests, such as the rollover, negative geotaxis, gait, and open field, assessed motor coordination and social interaction in offspring. VPA and VAD groups showed impaired behaviors, motor deficits, and elevated oxidative stress, marked by reduced superoxide dismutase, catalase, and glutathione levels, alongside increased malondialdehyde, nitrite oxide, and altered acetylcholine activity. VAS partially restored antioxidant defenses and alleviated behavioral symptoms. These findings highlight the exacerbating impact of VAD on ASD-like behaviors and the therapeutic potential of VAS in managing neurodevelopmental and biochemical alterations linked to ASD.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Efficient Deep Learning Framework for Automated Epileptic Seizure Detection: Toward Scalable and Clinically Applicable Solutions 用于自动癫痫发作检测的高效深度学习框架：面向可扩展和临床应用的解决方案

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-07 DOI: 10.1002/dneu.22983

Dezan Ji, Haozhou Cui, Haotian Li, Guoyang Liu, Zhen Liu, Wei Shang, Yi Li, Weidong Zhou

{"title":"An Efficient Deep Learning Framework for Automated Epileptic Seizure Detection: Toward Scalable and Clinically Applicable Solutions","authors":"Dezan Ji, Haozhou Cui, Haotian Li, Guoyang Liu, Zhen Liu, Wei Shang, Yi Li, Weidong Zhou","doi":"10.1002/dneu.22983","DOIUrl":"https://doi.org/10.1002/dneu.22983","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, we present an efficient epileptic seizure detection framework driven by a graph convolutional neural network (GCNN). Unlike conventional methods that primarily rely on local features or complex feature engineering, our GCNN-based approach explicitly encodes the spatial dependencies among electroencephalogram (EEG) electrodes, thereby capturing more comprehensive spatiotemporal features. A minimal preprocessing pipeline, consisting only of bandpass filtering and segmenting, reduces system complexity and computational overhead. On the CHB-MIT scalp EEG database, our method achieved an average accuracy of 98.64%, sensitivity of 99.49%, and specificity of 98.64% at the segment-based level and sensitivity of 96.81% with FDR of 0.27/h at the event-based level. On the SH-SDU database we collected, the method yielded segment-based accuracy of 95.23%, sensitivity of 92.42%, and specificity of 95.25%, along with event-based sensitivity of 94.11%. The average testing time for 1 h of multi-channel EEG signals is 3.89 s. These excellent results and low-computation design make the framework especially suited for clinical applications, advancing EEG-based epilepsy diagnostics and improving patient outcomes.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in Nanotechnology for Autism Spectrum Disorder: Innovative Strategies in Pediatric Neurology 纳米技术在自闭症谱系障碍中的进展：儿科神经病学的创新策略

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-07 DOI: 10.1002/dneu.22989

Haozhen Li, Jing Zhang

{"title":"Advancements in Nanotechnology for Autism Spectrum Disorder: Innovative Strategies in Pediatric Neurology","authors":"Haozhen Li, Jing Zhang","doi":"10.1002/dneu.22989","DOIUrl":"https://doi.org/10.1002/dneu.22989","url":null,"abstract":"<div>\u0000 \u0000 <p>Autism spectrum disorder (ASD) presents significant challenges in pediatric neurology, necessitating innovative management strategies to improve outcomes for affected children. This review explores the transformative potential of nanotechnology in autism treatment, highlighting specific applications of nanoscale materials and devices. We provide a detailed examination of various nanotechnology-based interventions, including targeted drug delivery systems that boost therapeutic efficacy, nanosensors for the early detection of ASD, and nanocarriers designed for gene therapy, all aimed at minimizing side effects while maximizing treatment benefits. Additionally, we discuss the role of nanotechnology in developing personalized medicine approaches tailored to the unique neurobiological profiles of children with ASD. By bridging the gap between research and clinical practice, this review aims to enhance the quality of care and life for pediatric patients with autism. We underscore the importance of interdisciplinary collaboration in advancing nanotechnology solutions and call for further research to validate these innovative strategies in pediatric neurology.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Roles of the Numb Protein in Synaptic Development and Plasticity 麻木蛋白在突触发育和可塑性中的作用

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-07-07 DOI: 10.1002/dneu.22988

Tatiana Sukhanova, M. Katie Renkemeyer, Nicholas Pritchett, Brett Berke, Haig Keshishian

{"title":"The Roles of the Numb Protein in Synaptic Development and Plasticity","authors":"Tatiana Sukhanova, M. Katie Renkemeyer, Nicholas Pritchett, Brett Berke, Haig Keshishian","doi":"10.1002/dneu.22988","DOIUrl":"https://doi.org/10.1002/dneu.22988","url":null,"abstract":"<div>\u0000 \u0000 <p>Numb is an adaptor protein with functions that include the endocytic processing of activated growth factor receptors. As growth factor signaling contributes to the development and function of the <i>Drosophila</i> neuromuscular junction (NMJ), we examined whether Numb is present at the larval NMJ and whether it is required for the growth, physiology, and/or plasticity of this synapse. Antisera prepared against Numb protein labeled NMJ presynaptic boutons, and RNAi knockdown of Numb, when directed to the presynaptic side, reduced the size of the NMJ. This was accompanied by smaller excitatory junctional potentials with reduced synaptic quantal content. Numb loss of function also suppressed the activity-dependent expansion of the NMJ, suggesting a requirement for Numb in synaptic growth plasticity. Similar phenotypes have been described at the NMJ for mutations of the Type II BMP growth factor receptor gene <i>wishful thinking (wit)</i>. As Numb is known to participate in growth factor receptor signaling in other systems, we tested whether a genetic interaction exists between the <i>numb</i> and <i>wit</i> genes. We observed a reduction of NMJ size in double heterozygotes compared to the single heterozygote control, suggesting that Numb is a candidate for processing growth factor signals during synaptic development and plasticity at the larval NMJ.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperbaric Oxygen Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating Sodium Channels 高压氧通过调节钠通道减轻大鼠脑缺血再灌注损伤

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-06-26 DOI: 10.1002/dneu.22974

Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng

{"title":"Hyperbaric Oxygen Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating Sodium Channels","authors":"Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng","doi":"10.1002/dneu.22974","DOIUrl":"https://doi.org/10.1002/dneu.22974","url":null,"abstract":"<div>\u0000 \u0000 <p>Ischemic stroke accounts for the majority of stroke cases. Hyperbaric oxygen therapy (HBOT) is being increasingly used as a treatment for this condition, but its precise mechanisms of action remain incompletely elucidated. This study examined hyperbaric oxygen (HBO) effects on sodium channels in hippocampal CA1 pyramidal neurons in rats with cerebral ischemia-reperfusion injury (CIRI). Using the middle cerebral artery occlusion (MCAO) model and whole-cell patch-clamp technology, voltage-gated sodium channel (VGSC) currents were measured at varying HBOT time points and treatment durations. Results showed that with a longer CIRI duration, the maximum current density (MCD) of sodium current (INa) decreased, while more HBOT sessions increased the MCD of INa. Notably, in the MCAO 6H group, 12 treatments of HBOT induced a leftward shift in the INa activation curve. Early HBOT intervention provided greater neuroprotection for sodium channels, and increased treatment sessions enhanced functional recovery. These findings suggest HBO's therapeutic potential in mitigating CIRI-related neuronal damage.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neonatal NLRP3 Inflammasome Activation Leads to Perineuronal Net Deficits in Early Adulthood 新生儿NLRP3炎性体激活导致成年早期神经元周围净缺陷

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-06-19 DOI: 10.1002/dneu.22986

Emre Tarakcioglu, Bilgesu Genc, Kemal Ugur Tufekci, Sermin Genc

{"title":"Neonatal NLRP3 Inflammasome Activation Leads to Perineuronal Net Deficits in Early Adulthood","authors":"Emre Tarakcioglu, Bilgesu Genc, Kemal Ugur Tufekci, Sermin Genc","doi":"10.1002/dneu.22986","DOIUrl":"https://doi.org/10.1002/dneu.22986","url":null,"abstract":"<p>Perineuronal nets (PNNs) are specialized extracellular matrix structures that surround certain neurons and play a critical role in protecting neurons from oxidative stress and maintaining synaptic stability in the central nervous system. They have roles in memory formation, and their loss has been linked to various mental alterations, such as anxiety, depression, and schizophrenia. While immune activation is known to degrade PNNs, it remains unclear whether inflammasomes are involved in PNN formation dynamics during neuronal development, where cases of sepsis are particularly high. In this study, we investigated how activation of the NLRP3 inflammasome in neonatal mouse brains influences PNNs. To explore this, neonatal wild-type and <i>Nlrp3</i> knockout mice were injected with lipopolysaccharide (LPS) or phosphate-buffered saline (PBS) on postnatal day (PND) 9, and PNNs were visualized at early adulthood (PND60). In addition, NLRP3 inflammasome activation was confirmed on PND10, and behavioral tests were performed on PND60. LPS treatment in wild-type mice reduced PNN-positive neurons in the hippocampus and cortex compared to the PBS group, whereas <i>Nlrp3</i> knockout mice showed no differences between treatment groups. Moreover, behavioral tests revealed that neonatal LPS injection resulted in anxiety- and depressive-like behavior and that NLRP3 deficiency restrained this effect. These results highlight the key role of NLRP3 inflammasome activation in inflammation-driven PNN reduction during neuronal development. NLRP3 inhibitors could thus serve as potential therapeutic agents to protect the neuronal extracellular matrix from inflammatory damage in early life.</p>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upregulated Expression and Shifted Distribution of Melatonin and Its Synthesizing Enzymes From Postnatal to Young Adult Rat Cochleae 褪黑素及其合成酶在出生后至年轻成年大鼠耳蜗中的表达上调及分布转移

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-06-18 DOI: 10.1002/dneu.22979

Monika Orsolic, Marc Diensthuber, Timo Stöver, Christin Geißler

{"title":"Upregulated Expression and Shifted Distribution of Melatonin and Its Synthesizing Enzymes From Postnatal to Young Adult Rat Cochleae","authors":"Monika Orsolic, Marc Diensthuber, Timo Stöver, Christin Geißler","doi":"10.1002/dneu.22979","DOIUrl":"https://doi.org/10.1002/dneu.22979","url":null,"abstract":"<p>The cochlea's cellular architecture plays a critical role in auditory perception, yet is highly susceptible to degenerative factors. While melatonin is known for its antioxidative properties in the adult cochlea, its expression during early development remains understudied. This study used immunohistochemical staining of melatonin and its synthesizing enzymes (AANAT, HIOMT) to explore the self-synthesis and spatial distribution of melatonin in the cochlea of postnatal and adult rats. Postnatal rats exhibited low levels of intracellular marker expression in the lateral wall and the undifferentiated sensory epithelia, with no expression observed in the spiral ganglion. They showed mainly extracellular marker expression near the stria vascularis, in the stria vascularis interspace, and above undifferentiated sensory epithelia. In contrast, adults exhibited widespread intracellular marker presence in spiral ligament fibrocytes, spiral ganglion neurons, satellite glia, and epithelial supporter cells, except in hair cells. Fibrocytes in the spiral limbus expressed AANAT and HIOMT at both developmental stages. The present findings indicate melatonin's complex involvement in cochlear protection and development. Detailed knowledge of melatonin synthesis locations and intensity across different age stages within the auditory system holds the key to pioneering novel treatments, preventive strategies, and a deeper understanding of hearing processes.</p>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22979","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of Caffeine on Siesta and Nighttime Sleep in Drosophila melanogaster 咖啡因对黑腹果蝇午睡和夜间睡眠的影响

IF 2.7 4区医学

Developmental Neurobiology Pub Date : 2025-06-17 DOI: 10.1002/dneu.22987

Debarati Bhattacharya, Terence L. Al Abaquita, Jolanta Górska-Andrzejak, Elzbieta Pyza

{"title":"The Influence of Caffeine on Siesta and Nighttime Sleep in Drosophila melanogaster","authors":"Debarati Bhattacharya, Terence L. Al Abaquita, Jolanta Górska-Andrzejak, Elzbieta Pyza","doi":"10.1002/dneu.22987","DOIUrl":"https://doi.org/10.1002/dneu.22987","url":null,"abstract":"<div>\u0000 \u0000 <p>Caffeine, a plant-derived psychostimulant, has been demonstrated to reduce sleep and increase dopaminergic neuron activity in mammals through competitive antagonism at adenosine receptors (AdoRs). However, the extent to which it influences daytime sleep <i>(siesta</i>) as much as nighttime sleep and the involvement of the adenosine signaling pathway in this process remain unsolved. It is similarly unclear whether the influence of caffeine varies with age and depends on sex and what type of cells it affects most; the clock cells that are involved in sleep timing and regulation or dopaminergic neurons, which are crucial for voluntary movement. To address this question, the role of caffeine has been investigated in <i>Drosophila melanogaster</i> (wild-type Canton-S flies), which has also been observed to reduce sleep in response to caffeine. The <i>siesta</i> and nighttime sleep of 3-, 30-, and 50-day-old males and females of Canton-S <i>Drosophila</i> were examined. Furthermore, transgenic flies with overexpressed or silenced <i>dAdoR</i> in all neurons (<i>elav</i>-expressing cells), the circadian clock cells (<i>tim</i>-expressing cells), and dopaminergic neurons (<i>th</i>-expressing cells) were studied. We found that females exhibited greater sensitivity to caffeine than males, and that older flies slept longer during the day than young flies. However, caffeine treatment resulted in reducing <i>siesta</i> in flies with overexpression of <i>dAdoR</i> in all neurons, <i>tim</i>-, and <i>th</i>-expressing cells. Conversely, silencing of <i>dAdoR</i> increased <i>siesta</i>. Therefore, the observed differences in the daytime sleep of <i>Drosophila</i> appeared to depend on signaling through AdoRs.</p>\u0000 </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0