Hyperbaric Oxygen Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating Sodium Channels

IF 2.7 4区 医学 Q2 DEVELOPMENTAL BIOLOGY
Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng
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Abstract

Ischemic stroke accounts for the majority of stroke cases. Hyperbaric oxygen therapy (HBOT) is being increasingly used as a treatment for this condition, but its precise mechanisms of action remain incompletely elucidated. This study examined hyperbaric oxygen (HBO) effects on sodium channels in hippocampal CA1 pyramidal neurons in rats with cerebral ischemia-reperfusion injury (CIRI). Using the middle cerebral artery occlusion (MCAO) model and whole-cell patch-clamp technology, voltage-gated sodium channel (VGSC) currents were measured at varying HBOT time points and treatment durations. Results showed that with a longer CIRI duration, the maximum current density (MCD) of sodium current (INa) decreased, while more HBOT sessions increased the MCD of INa. Notably, in the MCAO 6H group, 12 treatments of HBOT induced a leftward shift in the INa activation curve. Early HBOT intervention provided greater neuroprotection for sodium channels, and increased treatment sessions enhanced functional recovery. These findings suggest HBO's therapeutic potential in mitigating CIRI-related neuronal damage.

高压氧通过调节钠通道减轻大鼠脑缺血再灌注损伤
缺血性中风占中风病例的大多数。高压氧治疗(HBOT)被越来越多地用于治疗这种疾病,但其确切的作用机制仍未完全阐明。研究了高压氧(HBO)对脑缺血再灌注损伤大鼠海马CA1锥体神经元钠离子通道的影响。采用大脑中动脉闭塞(MCAO)模型和全细胞膜片钳技术,测量不同HBOT时间点和治疗时间下的电压门控钠通道(VGSC)电流。结果表明,随着CIRI持续时间的延长,钠电流(INa)的最大电流密度(MCD)降低,而HBOT持续时间的增加使INa的最大电流密度(MCD)增加。值得注意的是,在MCAO 6H组中,12次HBOT处理诱导了INa激活曲线的左移。早期HBOT干预为钠通道提供了更大的神经保护,增加治疗时间可促进功能恢复。这些发现表明HBO在减轻ciri相关神经元损伤方面具有治疗潜力。
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来源期刊
Developmental Neurobiology
Developmental Neurobiology 生物-发育生物学
CiteScore
6.50
自引率
0.00%
发文量
45
审稿时长
4-8 weeks
期刊介绍: Developmental Neurobiology (previously the Journal of Neurobiology ) publishes original research articles on development, regeneration, repair and plasticity of the nervous system and on the ontogeny of behavior. High quality contributions in these areas are solicited, with an emphasis on experimental as opposed to purely descriptive work. The Journal also will consider manuscripts reporting novel approaches and techniques for the study of the development of the nervous system as well as occasional special issues on topics of significant current interest. We welcome suggestions on possible topics from our readers.
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