Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng
{"title":"高压氧通过调节钠通道减轻大鼠脑缺血再灌注损伤","authors":"Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng","doi":"10.1002/dneu.22974","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Ischemic stroke accounts for the majority of stroke cases. Hyperbaric oxygen therapy (HBOT) is being increasingly used as a treatment for this condition, but its precise mechanisms of action remain incompletely elucidated. This study examined hyperbaric oxygen (HBO) effects on sodium channels in hippocampal CA1 pyramidal neurons in rats with cerebral ischemia-reperfusion injury (CIRI). Using the middle cerebral artery occlusion (MCAO) model and whole-cell patch-clamp technology, voltage-gated sodium channel (VGSC) currents were measured at varying HBOT time points and treatment durations. Results showed that with a longer CIRI duration, the maximum current density (MCD) of sodium current (INa) decreased, while more HBOT sessions increased the MCD of INa. Notably, in the MCAO 6H group, 12 treatments of HBOT induced a leftward shift in the INa activation curve. Early HBOT intervention provided greater neuroprotection for sodium channels, and increased treatment sessions enhanced functional recovery. These findings suggest HBO's therapeutic potential in mitigating CIRI-related neuronal damage.</p>\n </div>","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":"85 3","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Hyperbaric Oxygen Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating Sodium Channels\",\"authors\":\"Binli Gao, Mei Peng, Fulan Chen, Xiaoling Peng, Ke Huang, Qiang Li, Yuee Dai, Xianrong Zeng\",\"doi\":\"10.1002/dneu.22974\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Ischemic stroke accounts for the majority of stroke cases. Hyperbaric oxygen therapy (HBOT) is being increasingly used as a treatment for this condition, but its precise mechanisms of action remain incompletely elucidated. This study examined hyperbaric oxygen (HBO) effects on sodium channels in hippocampal CA1 pyramidal neurons in rats with cerebral ischemia-reperfusion injury (CIRI). Using the middle cerebral artery occlusion (MCAO) model and whole-cell patch-clamp technology, voltage-gated sodium channel (VGSC) currents were measured at varying HBOT time points and treatment durations. Results showed that with a longer CIRI duration, the maximum current density (MCD) of sodium current (INa) decreased, while more HBOT sessions increased the MCD of INa. Notably, in the MCAO 6H group, 12 treatments of HBOT induced a leftward shift in the INa activation curve. Early HBOT intervention provided greater neuroprotection for sodium channels, and increased treatment sessions enhanced functional recovery. These findings suggest HBO's therapeutic potential in mitigating CIRI-related neuronal damage.</p>\\n </div>\",\"PeriodicalId\":11300,\"journal\":{\"name\":\"Developmental Neurobiology\",\"volume\":\"85 3\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental Neurobiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dneu.22974\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Neurobiology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dneu.22974","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Hyperbaric Oxygen Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating Sodium Channels
Ischemic stroke accounts for the majority of stroke cases. Hyperbaric oxygen therapy (HBOT) is being increasingly used as a treatment for this condition, but its precise mechanisms of action remain incompletely elucidated. This study examined hyperbaric oxygen (HBO) effects on sodium channels in hippocampal CA1 pyramidal neurons in rats with cerebral ischemia-reperfusion injury (CIRI). Using the middle cerebral artery occlusion (MCAO) model and whole-cell patch-clamp technology, voltage-gated sodium channel (VGSC) currents were measured at varying HBOT time points and treatment durations. Results showed that with a longer CIRI duration, the maximum current density (MCD) of sodium current (INa) decreased, while more HBOT sessions increased the MCD of INa. Notably, in the MCAO 6H group, 12 treatments of HBOT induced a leftward shift in the INa activation curve. Early HBOT intervention provided greater neuroprotection for sodium channels, and increased treatment sessions enhanced functional recovery. These findings suggest HBO's therapeutic potential in mitigating CIRI-related neuronal damage.
期刊介绍:
Developmental Neurobiology (previously the Journal of Neurobiology ) publishes original research articles on development, regeneration, repair and plasticity of the nervous system and on the ontogeny of behavior. High quality contributions in these areas are solicited, with an emphasis on experimental as opposed to purely descriptive work. The Journal also will consider manuscripts reporting novel approaches and techniques for the study of the development of the nervous system as well as occasional special issues on topics of significant current interest. We welcome suggestions on possible topics from our readers.