Developmental Neurobiology最新文献_第6页

The role of snare proteins in cortical development 网罗蛋白在皮质发育中的作用

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-06-20 DOI: 10.1002/dneu.22892

Auguste Vadisiute, Elise Meijer, Florina Szabó, Anna Hoerder-Suabedissen, Eri Kawashita, Shuichi Hayashi, Zoltán Molnár

{"title":"The role of snare proteins in cortical development","authors":"Auguste Vadisiute, Elise Meijer, Florina Szabó, Anna Hoerder-Suabedissen, Eri Kawashita, Shuichi Hayashi, Zoltán Molnár","doi":"10.1002/dneu.22892","DOIUrl":"10.1002/dneu.22892","url":null,"abstract":"Neural communication in the adult nervous system is mediated primarily through chemical synapses, where action potentials elicit Ca2+ signals, which trigger vesicular fusion and neurotransmitter release in the presynaptic compartment. At early stages of development, the brain is shaped by communication via trophic factors and other extracellular signaling, and by contact-mediated cell–cell interactions including chemical synapses. The patterns of early neuronal impulses and spontaneous and regulated neurotransmitter release guide the precise topography of axonal projections and contribute to determining cell survival. The study of the role of specific proteins of the synaptic vesicle release machinery in the establishment, plasticity, and maintenance of neuronal connections during development has only recently become possible, with the advent of mouse models where various members of the N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex have been genetically manipulated. We provide an overview of these models, focusing on the role of regulated vesicular release and/or cellular excitability in synaptic assembly, development and maintenance of cortical circuits, cell survival, circuit level excitation–inhibition balance, myelination, refinement, and plasticity of key axonal projections from the cerebral cortex. These models are important for understanding various developmental and psychiatric conditions, and neurodegenerative diseases.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evolution of genetic mechanisms regulating cortical neurogenesis 调节皮层神经发生的遗传机制的进化

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-06-07 DOI: 10.1002/dneu.22891

Alexandre Espinós, Eduardo Fernández-Ortuño, Enrico Negri, Víctor Borrell

{"title":"Evolution of genetic mechanisms regulating cortical neurogenesis","authors":"Alexandre Espinós, Eduardo Fernández-Ortuño, Enrico Negri, Víctor Borrell","doi":"10.1002/dneu.22891","DOIUrl":"10.1002/dneu.22891","url":null,"abstract":"The size of the cerebral cortex increases dramatically across amniotes, from reptiles to great apes. This is primarily due to different numbers of neurons and glial cells produced during embryonic development. The evolutionary expansion of cortical neurogenesis was linked to changes in neural stem and progenitor cells, which acquired increased capacity of self-amplification and neuron production. Evolution works via changes in the genome, and recent studies have identified a small number of new genes that emerged in the recent human and primate lineages, promoting cortical progenitor proliferation and increased neurogenesis. However, most of the mammalian genome corresponds to noncoding DNA that contains gene-regulatory elements, and recent evidence precisely points at changes in expression levels of conserved genes as key in the evolution of cortical neurogenesis. Here, we provide an overview of basic cellular mechanisms involved in cortical neurogenesis across amniotes, and discuss recent progress on genetic mechanisms that may have changed during evolution, including gene expression regulation, leading to the expansion of the cerebral cortex.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49498401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Evaluation of advances in cortical development using model systems 用模型系统评价皮层发育的进展

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-05-29 DOI: 10.1002/dneu.22879

Patricia R. Nano, Aparna Bhaduri

{"title":"Evaluation of advances in cortical development using model systems","authors":"Patricia R. Nano, Aparna Bhaduri","doi":"10.1002/dneu.22879","DOIUrl":"10.1002/dneu.22879","url":null,"abstract":"Compared with that of even the closest primates, the human cortex displays a high degree of specialization and expansion that largely emerges developmentally. Although decades of research in the mouse and other model systems has revealed core tenets of cortical development that are well preserved across mammalian species, small deviations in transcription factor expression, novel cell types in primates and/or humans, and unique cortical architecture distinguish the human cortex. Importantly, many of the genes and signaling pathways thought to drive human-specific cortical expansion also leave the brain vulnerable to disease, as the misregulation of these factors is highly correlated with neurodevelopmental and neuropsychiatric disorders. However, creating a comprehensive understanding of human-specific cognition and disease remains challenging. Here, we review key stages of cortical development and highlight known or possible differences between model systems and the developing human brain. By identifying the developmental trajectories that may facilitate uniquely human traits, we highlight open questions in need of approaches to examine these processes in a human context and reveal translatable insights into human developmental disorders.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9835301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transcriptomic analyses of NeuroD1-mediated astrocyte-to-neuron conversion NeuroD1介导的星形胶质细胞向神经元转化的转录组学分析

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-05-23 DOI: 10.1002/dneu.22882

Ning-Xin Ma, Brendan Puls, Gong Chen

{"title":"Transcriptomic analyses of NeuroD1-mediated astrocyte-to-neuron conversion","authors":"Ning-Xin Ma, Brendan Puls, Gong Chen","doi":"10.1002/dneu.22882","DOIUrl":"10.1002/dneu.22882","url":null,"abstract":"Ectopic expression of a single neural transcription factor NeuroD1 can reprogram reactive glial cells into functional neurons both in vitro and in vivo, but the underlying mechanisms are not well understood yet. Here, we used RNA-sequencing technology to capture the transcriptomic changes at different time points during the reprogramming process. We found that following NeuroD1 overexpression, astroglial genes (ACTG1, ALDH1A3, EMP1, CLDN6, SOX21) were significantly downregulated, whereas neuronal genes (DCX, RBFOX3/NeuN, CUX2, RELN, SNAP25) were significantly upregulated. NeuroD family members (NeuroD1/2/6) and signaling pathways (Wnt, MAPK, cAMP) as well as neurotransmitter receptors (acetylcholine, somatostatin, dopamine) were also significantly upregulated. Gene co-expression analysis identified many central genes among the NeuroD1-interacting network, including CABP7, KIAA1456, SSTR2, GADD45G, LRRTM2, and INSM1. Compared to chemical conversion, we found that NeuroD1 acted as a strong driving force and triggered fast transcriptomic changes during astrocyte-to-neuron conversion process. Together, this study reveals many important downstream targets of NeuroD1 such as HES6, BHLHE22, INSM1, CHRNA1/3, CABP7, and SSTR2, which may play critical roles during the transcriptomic landscape shift from a glial profile to a neuronal profile.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22882","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42936637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

In vivo glia-to-neuron conversion: pitfalls and solutions 体内胶质细胞到神经元的转换:陷阱和解决方案

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-05-10 DOI: 10.1002/dneu.22880

Lei-Lei Wang, Chun-Li Zhang

引用次数: 11

E3 ubiquitin ligases and cerebral cortex development in health and disease E3泛素连接酶与健康和疾病中的大脑皮层发育

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-04-27 DOI: 10.1002/dneu.22877

Nicolas Lambert, Martin Moïse, Laurent Nguyen

引用次数: 0

Characterization of neurological disease progression in a canine model of CLN5 neuronal ceroid lipofuscinosis 犬CLN5神经元样脂褐变模型神经系统疾病进展的表征

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-04-15 DOI: 10.1002/dneu.22878

Elizabeth J. Meiman, Grace Robinson Kick, Cheryl A. Jensen, Joan R. Coates, Martin L. Katz

{"title":"Characterization of neurological disease progression in a canine model of CLN5 neuronal ceroid lipofuscinosis","authors":"Elizabeth J. Meiman, Grace Robinson Kick, Cheryl A. Jensen, Joan R. Coates, Martin L. Katz","doi":"10.1002/dneu.22878","DOIUrl":"10.1002/dneu.22878","url":null,"abstract":"Golden Retriever dogs with a frameshift variant in CLN5 (c.934_935delAG) suffer from a progressive neurodegenerative disorder analogous to the CLN5 form of neuronal ceroid lipofuscinosis (NCL). Five littermate puppies homozygous for the deletion allele were identified prior to the onset of disease signs. Studies were performed to characterize the onset and progression of the disease in these dogs. Neurological signs that included restlessness, unwillingness to cooperate with the handlers, and proprioceptive deficits first became apparent at approximately 12 months of age. The neurological signs progressed over time and by 21 to 23 months of age included general proprioceptive ataxia, menace response deficits, aggressive behaviors, cerebellar ataxia, intention tremors, decreased visual tracking, seizures, cognitive decline, and impaired prehension. Due to the severity of these signs, the dogs were euthanized between 21 and 23 months of age. Magnetic resonance imaging revealed pronounced progressive global brain atrophy with a more than sevenfold increase in the volume of the ventricular system between 9.5 and 22.5 months of age. Accompanying this atrophy were pronounced accumulations of autofluorescent inclusions throughout the brain and spinal cord. Ultrastructurally, the contents of these inclusions were found to consist primarily of membrane-like aggregates. Inclusions with similar fluorescence properties were present in cardiac muscle. Similar to other forms of NCL, the affected dogs had low plasma carnitine concentrations, suggesting impaired carnitine biosynthesis. These data on disease progression will be useful in future studies using the canine model for therapeutic intervention studies.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22878","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42405973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Refinement of axonal conduction and myelination in the mouse optic nerve indicate an extended period of postnatal developmental plasticity 小鼠视神经的轴突传导和髓鞘形成的改善表明了出生后发育可塑性的延长

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-04-11 DOI: 10.1002/dneu.22875

Annika Balraj, Cheryl Clarkson-Paredes, Ahdeah Pajoohesh-Ganji, Matthew W. Kay, David Mendelowitz, Robert H. Miller

{"title":"Refinement of axonal conduction and myelination in the mouse optic nerve indicate an extended period of postnatal developmental plasticity","authors":"Annika Balraj, Cheryl Clarkson-Paredes, Ahdeah Pajoohesh-Ganji, Matthew W. Kay, David Mendelowitz, Robert H. Miller","doi":"10.1002/dneu.22875","DOIUrl":"10.1002/dneu.22875","url":null,"abstract":"Retinal ganglion cells generate a pattern of action potentials to communicate visual information from the retina to cortical areas. Myelin, an insulating sheath, wraps axonal segments to facilitate signal propagation and when deficient, can impair visual function. Optic nerve development and initial myelination has largely been considered completed by the fifth postnatal week. However, the relationship between the extent of myelination and axonal signaling in the maturing optic nerve is not well characterized. Here, we examine the relationship between axon conduction and elements of myelination using extracellular nerve recordings, immunohistochemistry, western blot analysis, scanning electron microscopy, and simulations of nerve responses. Comparing compound action potentials from mice aged 4–12 weeks revealed five functional distinct axonal populations, an increase in the number of functional axons, and shifts toward fast-conducting axon populations at 5 and 8 weeks postnatal. At these ages, our analysis revealed increased myelin thickness, lower g-ratios and changes in the 14 kDa MBP isoform, while the density of axons and nodes of Ranvier remained constant. At 5 postnatal weeks, axon diameter increased, while at 8 weeks, increased expression of a mature sodium ion channel subtype, Nav 1.6, was observed at nodes of Ranvier. A simulation model of nerve conduction suggests that ion channel subtype, axon diameter, and myelin thickness are more likely to be key regulators of nerve function than g-ratio. Such refinement of axonal function and myelin rearrangement identified an extended period of maturation in the normal optic nerve that may facilitate the development of visual signaling patterns.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9372870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Polycomb-mediated gene regulation in human brain development and neurodevelopmental disorders polycomb介导的基因调控在人脑发育和神经发育障碍中的作用

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-04-05 DOI: 10.1002/dneu.22876

Nora Bölicke, Mareike Albert

{"title":"Polycomb-mediated gene regulation in human brain development and neurodevelopmental disorders","authors":"Nora Bölicke, Mareike Albert","doi":"10.1002/dneu.22876","DOIUrl":"10.1002/dneu.22876","url":null,"abstract":"The neocortex is considered the seat of higher cognitive function in humans. It develops from a sheet of neural progenitor cells, most of which eventually give rise to neurons. This process of cell fate determination is controlled by precise temporal and spatial gene expression patterns that in turn are affected by epigenetic mechanisms including Polycomb group (PcG) regulation. PcG proteins assemble in multiprotein complexes and catalyze repressive posttranslational histone modifications. Their association with neurodevelopmental disease and various types of cancer of the central nervous system, as well as observations in mouse models, has implicated these epigenetic modifiers in controlling various stages of cortex development. The precise mechanisms conveying PcG‐associated transcriptional repression remain incompletely understood and are an active field of research. PcG activity appears to be highly context‐specific, raising the question of species‐specific differences in the regulation of neural stem and progenitor regulation. In this review, we will discuss our growing understanding of how PcG regulation affects human cortex development, based on studies in murine model systems, but focusing mostly on findings obtained from examining impaired PcG activity in the context of human neurodevelopmental disorders and cancer. Furthermore, we will highlight relevant experimental approaches for functional investigations of PcG regulation in human cortex development.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22876","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48595924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cortical myelination in toddlers and preschoolers with autism spectrum disorder 患有自闭症谱系障碍的幼儿和学龄前儿童的皮层髓鞘形成

IF 3 4区医学

Developmental Neurobiology Pub Date : 2022-03-29 DOI: 10.1002/dneu.22874

Bosi Chen, Annika Linke, Lindsay Olson, Jiwandeep Kohli, Mikaela Kinnear, Martin Sereno, Ralph-Axel Müller, Ruth Carper, Inna Fishman

{"title":"Cortical myelination in toddlers and preschoolers with autism spectrum disorder","authors":"Bosi Chen, Annika Linke, Lindsay Olson, Jiwandeep Kohli, Mikaela Kinnear, Martin Sereno, Ralph-Axel Müller, Ruth Carper, Inna Fishman","doi":"10.1002/dneu.22874","DOIUrl":"10.1002/dneu.22874","url":null,"abstract":"Intracortical myelin is thought to play a significant role in the development of neural circuits and functional networks, with consistent evidence of atypical network connectivity in children with autism spectrum disorder (ASD). However, little is known about the development of intracortical myelin in the first years of life in ASD, during the critical neurodevelopmental period when autism symptoms first emerge. Using T1-weighted (T1w) and T2w structural magnetic resonance imaging (MRI) in 21 young children with ASD and 16 typically developing (TD) children, ages 1.5–5.5 years, we demonstrate the feasibility of estimating intracortical myelin in vivo using the T1w/T2w ratio as a proxy. The resultant T1w/T2w maps were largely comparable with those reported in prior T1w/T2w studies in TD children and adults, and revealed no group differences between TD children and those with ASD. However, differential associations between T1w/T2w and age were identified in several early myelinated regions (e.g., visual, posterior cingulate, precuneus cortices) in the ASD and TD groups, with age-related increase in estimated myelin content across the toddler and preschool years detected in TD children, but not in children with ASD. The atypical age-related effects in intracortical myelin, suggesting a disrupted myelination in the first years of life in ASD, may be related to the aberrant brain network connectivity reported in young children with ASD in some of the same cortical regions and circuits.","PeriodicalId":11300,"journal":{"name":"Developmental Neurobiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/dneu.22874","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41621953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7