Diseases of aquatic organisms最新文献_第7页

Spatio-temporal patterns of Perkinsus marinus infections are driven by a changing environment in the Chesapeake Bay. 切萨皮克湾环境的变化驱动了海扁虱感染的时空格局。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-11-20 DOI: 10.3354/dao03876

Mariah L Kachmar, Chelsea Bergman, Harold J Schreier, Gemma Feild, Katrina M Pagenkopp Lohan, Ryan B Carnegie, Colleen A Burge, Sarah Gignoux-Wolfsohn

{"title":"Spatio-temporal patterns of Perkinsus marinus infections are driven by a changing environment in the Chesapeake Bay.","authors":"Mariah L Kachmar, Chelsea Bergman, Harold J Schreier, Gemma Feild, Katrina M Pagenkopp Lohan, Ryan B Carnegie, Colleen A Burge, Sarah Gignoux-Wolfsohn","doi":"10.3354/dao03876","DOIUrl":"https://doi.org/10.3354/dao03876","url":null,"abstract":"Shellfish fisheries and aquaculture within the Chesapeake Bay (hereafter 'the Bay') and its tributaries have been historically impacted by disease and climate events. Climate-driven shifts in temperature and salinity can alter host-parasite dynamics, influencing outbreaks. Here, we explore the relationship between temperature, salinity and parasite distribution and abundance in the eastern oyster Crassostrea virginica-Perkinsus marinus system. We use long-term (30 yr) environmental data and P. marinus surveys in the Bay to identify (1) how climate affects P. marinus prevalence and intensity, (2) seasonal and climate-driven infection patterns, and (3) regional environmental influences on disease. We found significant relationships between P. marinus infection intensity, prevalence, increasing temperature and decreasing salinity. Our results indicated that there is an overall decreased abundance of P. marinus prevalence and intensity throughout the Bay driven by decreases in salinity over time, most prominently from 2003-2020. However, these temporal trends in prevalence and intensity vary largely by region, with some regions still experiencing high disease burden. Examining monthly environmental parameters reinforced the dominant role of salinity in driving disease patterns. Salinity had significant relationships with prevalence and intensity year-round, with the largest effects in late spring/early summer. Monthly temperatures had fewer significant relationships to prevalence and intensity, but the largest significant effects were seen in late winter/early spring. Notably, this study is the first to document that winter salinity influences fall parasite prevalence, sometimes exerting a greater effect than temperature. Continued and expanded monitoring of marine disease is crucial to understand how the changing climate is impacting disease.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"111-127"},"PeriodicalIF":1.2,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145563044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First occurrence of ranavirus in the Scandinavian peninsula. 斯堪的纳维亚半岛首次出现拉纳病毒。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-11-20 DOI: 10.3354/dao03879

B Thumsová, N Chondrelli, A E Valdés, R Eghbal, J Höglund, A Laurila, J Bosch, M Cortázar-Chinarro

引用次数: 0

Antiviral potential of aqueous extract of star anise Illicium verum against fish nodavirus. 八角茴香水提物对鱼类诺达病毒的抗病毒作用。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-11-06 DOI: 10.3354/dao03869

Mohiadeen Shajia Banu, Selvam Suryakodi, Ratan Kumar Saha, Sivaraj Mithra, Seepoo Abdul Majeed, Gani Taju, Azeez Sait Hameed, Himadri Saha

{"title":"Antiviral potential of aqueous extract of star anise Illicium verum against fish nodavirus.","authors":"Mohiadeen Shajia Banu, Selvam Suryakodi, Ratan Kumar Saha, Sivaraj Mithra, Seepoo Abdul Majeed, Gani Taju, Azeez Sait Hameed, Himadri Saha","doi":"10.3354/dao03869","DOIUrl":"https://doi.org/10.3354/dao03869","url":null,"abstract":"We tested the antiviral activity of aqueous extract of star anise Illicium verum (AEIV) against striped jack nervous necrosis virus (SJNNV) in vitro as well as in vivo using the Sahul Indian Seabass Spleen (SISS) cell line and Asian seabass Lates calcarifer. Preliminary results from GC-MS profiling and polyphenol content estimation of AEIV revealed the antiviral potential of the extract. Before testing the compound, the extract was assessed for its toxic potential in an in vitro model (SISS cell line) using 4 different concentrations (50, 75, 100 and 200 µg ml-1) of AEIV through MTT and neutral red assays. The tested concentrations were not toxic (≥90% survival). Subsequently, the same concentrations of AEIV were tested for their antiviral potential against SJNNV in SISS cells. The results showed that AEIV at higher concentration (100 and 200 µg ml-1) effectively inhibited SJNNV replication in the SISS cells. RT-PCR and qPCR confirmed the inhibition of the virus. The same concentrations were then used to evaluate their antiviral efficacy in vivo by intraperitoneal injection in Asian seabass. We observed 100% mortality only in the fish injected with SJNNV (control), which confirms that AEIV possesses the potency to control the replication of SJNNV under both in vitro and in vivo conditions. The present study indicates that medication with plant extracts having antiviral activity could be a potentially beneficial alternative to the usage of chemotherapeutics in aquaculture.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"91-103"},"PeriodicalIF":1.2,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hydrogen peroxide on microcystin-LR from Microcystis sp. 过氧化氢对微囊藻中微囊藻毒素lr的影响。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-11-06 DOI: 10.3354/dao03875

Pok Him Ng, Sophie St-Hilaire

{"title":"Effect of hydrogen peroxide on microcystin-LR from Microcystis sp.","authors":"Pok Him Ng, Sophie St-Hilaire","doi":"10.3354/dao03875","DOIUrl":"https://doi.org/10.3354/dao03875","url":null,"abstract":"Microcystis spp. blooms in fish ponds can lead to fluctuations in water parameters, and the microcystin-LR (MC-LR) toxin they produce may pose health risks for fish, as well as consumers. Hydrogen peroxide (H2O2) treatment has been demonstrated to reduce populations of Microcystis spp. in aquaculture ponds. This study conducted 2 trials to investigate whether these treatments result in the release of MC-LR under laboratory conditions. We applied 7 mg l-1 of H2O2 to a Microcystis sp. culture and assessed the MC-LR level post-treatment. In the first trial, no MC-LR was detected in the H2O2 treatment group nor the negative control group, while 0.933 ± 0.381 ppb of MC-LR was extracted from 2344230 Microcystis sp. cells in 5 ml of media stock in our positive control group (where all cells were destroyed). In our second trial, where we added almost 30 times more Microcystis sp. to the culture media (i.e. 64125000 cells in 5 ml of stock), 19.333 ± 0.742 ppb of MC-LR were detected in the positive control on average. At this concentration of algae, our negative control (no treatment) and our H2O2 treatment group had 16.933 ± 0.303 ppb and 16.933 ± 0.109 ppb of MC-LR, respectively. The toxin levels were similar between the treated and untreated groups, but significantly less than the positive control group (p = 0.003). The results suggest that 7 mg l-1 H2O2 treatment to Microcystis sp. did not increase the level of MC-LR in the solution, but MC-LR was also not removed by the treatment. The low-dose 7 mg l-1 H2O2 treatment against Microcystis sp. bloom is unlikely to cause extra toxin (MC-LR) release to the medium.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"105-109"},"PeriodicalIF":1.2,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dermisichthinium pseudosporum gen. et sp. nov. (Dinophyceae, Suessiaceae): a dinoflagellate parasite in freshwater fish in Wisconsin, USA. 假孢子甲藻（dinmisichthinium pseudosporum gen. et sp. 11 .）：美国威斯康辛州淡水鱼中甲藻寄生虫。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-23 DOI: 10.3354/dao03874

Jonah A Nguyen, Justin M Stilwell, Sarah Sanderson, Nicole A Nietlisbach, Christopher Long, Eric Leis, Elisabeth Hehenberger, Ethan T Woodyard, Matt J Griffin, Thomas G Rosser

{"title":"Dermisichthinium pseudosporum gen. et sp. nov. (Dinophyceae, Suessiaceae): a dinoflagellate parasite in freshwater fish in Wisconsin, USA.","authors":"Jonah A Nguyen, Justin M Stilwell, Sarah Sanderson, Nicole A Nietlisbach, Christopher Long, Eric Leis, Elisabeth Hehenberger, Ethan T Woodyard, Matt J Griffin, Thomas G Rosser","doi":"10.3354/dao03874","DOIUrl":"https://doi.org/10.3354/dao03874","url":null,"abstract":"A novel dinoflagellate is described from bluegill Lepomis macrochirus, rock bass Ambloplites rupestris, largemouth bass Micropterus nigricans, and yellow perch Perca flavescens collected from Lundgren Lake and Townsend Flowage, Wisconsin, USA. A new genus, Dermisichthinium gen. nov., is established for this species, D. pseudosporum sp. nov., which produces white spots grossly similar to those caused by Ichthyophthirius multifiliis. Microscopically, however, the vegetative cysts of D. pseudosporum closely resemble Haidadinium ichthyophilum, a parasite of threespine stickleback Gasterosteus aculeatus. Haidadinium ichthyophilum was collected from Haida Gwaii, British Columbia, Canada, for morphological and molecular comparison. Molecular analysis of the small subunit (18S), large subunit (28S), and internal transcribed spacer rDNA regions supports the novel species description and erection of a new genus. Pairwise comparisons of partial 18S and 28S sequences revealed divergence levels approximately 3 times greater than those among congeneric suessiacean dinoflagellates. Dermisichthinium pseudosporum sp. nov. lacks a 25 bp insertion in 28S unique to H. ichthyophilum, providing a molecular character for distinguishing the 2 species and supporting their placement in separate genera. Phylogenetic analyses consistently place D. pseudosporum sp. nov. and H. ichthyophilum in distinct clades. This study enhances our understanding of parasitic dinoflagellate diversity, underscores the importance of integrating morphological, molecular, and other diagnostic characteristics in their taxonomic classification, and offers valuable diagnostic insight for fish health professionals and parasitologists encountering this unusual group of cyst-forming dinoflagellates.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"67-90"},"PeriodicalIF":1.2,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal modulation of proteasome and ubiquitin-mediated responses in rainbow trout kidneys during infectious pancreatic necrosis virus infection. 感染性胰腺坏死病毒感染期间虹鳟鱼肾脏蛋白酶体和泛素介导反应的时间调节。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-23 DOI: 10.3354/dao03872

Pamela Breguel-Serrano, Daniela Aravena-Canales, Rodrigo Zuloaga, Francisca Pérez-Molina, Andrea Soza, Juan A Valdés, Andrea Rivas-Aravena

{"title":"Temporal modulation of proteasome and ubiquitin-mediated responses in rainbow trout kidneys during infectious pancreatic necrosis virus infection.","authors":"Pamela Breguel-Serrano, Daniela Aravena-Canales, Rodrigo Zuloaga, Francisca Pérez-Molina, Andrea Soza, Juan A Valdés, Andrea Rivas-Aravena","doi":"10.3354/dao03872","DOIUrl":"https://doi.org/10.3354/dao03872","url":null,"abstract":"Infectious pancreatic necrosis virus (IPNV) poses a significant economic threat to aquaculture, yet understanding of the host-virus interaction remains limited. This study investigates the impact of IPNV on proteasomal response within rainbow trout kidneys during both subclinical (5 days post-infection, dpi) and clinical (10 dpi) stages of infection. Our results reveal a biphasic response: an initial stimulation of proteasomal activity at 5 dpi followed by inhibition at 10 dpi. Transcript analysis of proteasomal subunits in individual fish shows predominantly a correlation between their expression and proteasomal activity at 5 dpi, but this correlation was not seen by 10 dpi. Additionally, it was observed that the upregulation of immunoproteasome subunits by gamma interferon (γIFN) did not consistently cause a rise in proteasomal activity. Notably, a significant increase in free ubiquitin and ubiquitinated proteins was detected at 5 dpi, followed by a decrease at 10 dpi, indicative of dynamic changes in protein degradation, which agrees with proteasomal activity. The biphasic proteasome response suggests that early activation aids viral protein clearance, while later inhibition triggers cellular stress, potentially driving symptom development. Overall, this study underscores the significant impact of IPNV on proteasome activity in rainbow trout.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"45-56"},"PeriodicalIF":1.2,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a quantitative PCR TaqMan™ assay for the detection of Ergasilus labracis in mixed plankton samples from a Newfoundland bay. 定量PCR TaqMan™检测纽芬兰湾混合浮游生物样本中唇格缕虫的应用

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-23 DOI: 10.3354/dao03873

Harry M Murray, Kimberley D Hobbs

{"title":"Application of a quantitative PCR TaqMan™ assay for the detection of Ergasilus labracis in mixed plankton samples from a Newfoundland bay.","authors":"Harry M Murray, Kimberley D Hobbs","doi":"10.3354/dao03873","DOIUrl":"https://doi.org/10.3354/dao03873","url":null,"abstract":"Ergasilids are an important group of parasitic copepods that occur globally in some coastal, estuarine, and freshwater habitats, including the south coast of the island of Newfoundland, Canada. Generally, males and developing females are not parasitic and remain in plankton. Adult females, however, become parasitic and seek a host following mating. Few studies have focused on detection and/or quantification of planktonic stages, and of those, all have utilized microscopic techniques. This method is time consuming and dependent on a specific parasitological skill set. In recent years, quantitative PCR (qPCR) techniques have become common in the detection and relative quantification of various invertebrate larval stages within plankton, including many metazoan parasites. In the present study, a qPCR assay using TaqMan™ minor groove binder probe technology, based on the Ergasilus labracis mitochondrial cytochrome c oxidase subunit I sequence, was developed for the first time to detect this parasite in mixed plankton samples taken near active salmonid aquaculture sites in a Northwest Atlantic coastal estuary. Ultimately, the technique can be used for tracking seasonal variability and abundance of planktonic stages of this parasite and thus illustrate patterns of seasonal infestation for both wild and cultured species in this region.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"57-65"},"PeriodicalIF":1.2,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Timing of seasonal pathogen exposure relative to oyster lifecycle. 季节性病原体暴露时间与牡蛎生命周期的关系。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-09 DOI: 10.3354/dao03870

Justin Manley, Bonnie Brown, Thomas Bliss, Elizabeth L Harvey

{"title":"Timing of seasonal pathogen exposure relative to oyster lifecycle.","authors":"Justin Manley, Bonnie Brown, Thomas Bliss, Elizabeth L Harvey","doi":"10.3354/dao03870","DOIUrl":"https://doi.org/10.3354/dao03870","url":null,"abstract":"Protozoan parasites Perkinsus marinus (dermo) and Haplosporidium nelsoni (MSX) are associated with extensive epizootic mortality in eastern oyster Crassostrea virginica populations along the coastlines of the Atlantic Ocean and Gulf of Mexico. For decades, both diseases have resulted in substantial losses to ecological functioning of natural oyster habitat and have undermined the economic stability of wild oyster fisheries and mariculture industries in the USA. Both pathogens are widespread in coastal Georgia and are established regionally within native oyster populations, yet a clear timeline of transmission is not defined. Here, high frequency sampling was used to investigate when disease transmission is most probable relative to oyster lifecycle stage based in a localized region of the South Atlantic Bight (SAB). Dermo was present in the water column throughout the year with peaks in abundance concurrent with spawning activity, resulting in significant positive correlations among P. marinus concentration and the number of spawning females, egg yield, and shell height per spawning female. Detection of MSX in water samples appeared during 3 punctuated periods throughout the year but was not significantly associated with any environmental or life cycle metric. Overall, this research indicates that spawning events may be a mechanism for P. marinus transmission in oyster populations of the SAB.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"1-12"},"PeriodicalIF":1.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring ostreid herpesvirus-1 (OsHV-1) outbreaks in juvenile Pacific oysters Crassostrea gigas along the west coast of the USA. 监测OsHV-1在美国西海岸幼龄太平洋牡蛎长牡蛎中的爆发

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-09 DOI: 10.3354/dao03868

Sanford Evans, Christopher J Langdon, Colleen A Burge, Sukanya Dayal, Brett R Dumbauld

{"title":"Monitoring ostreid herpesvirus-1 (OsHV-1) outbreaks in juvenile Pacific oysters Crassostrea gigas along the west coast of the USA.","authors":"Sanford Evans, Christopher J Langdon, Colleen A Burge, Sukanya Dayal, Brett R Dumbauld","doi":"10.3354/dao03868","DOIUrl":"https://doi.org/10.3354/dao03868","url":null,"abstract":"Ostreid herpesvirus-1 (OsHV-1) is a threat to the global production of Pacific oysters Crassostrea gigas, often resulting in nearly complete mortality in affected stocks. A sentinel monitoring program was conducted between June and October 2020, to characterize OsHV-1 outbreaks in Pacific oysters along the west coast of the USA. Deployment of sentinel oysters at 5 commercial growing locations, coupled with frequent sampling, allowed measurement of the spatial and temporal occurrence of OsHV-1 outbreaks as well as the viral load and pathogenesis of OsHV-1 during C. gigas mortality events. In addition, 2 divergent oyster families were deployed at sites that have historically tested positive for OsHV-1 to measure the effect of oyster genotype on the severity of OsHV-1-induced mortality in the field. Mortality events at California test sites were associated with elevated levels of OsHV-1 in oyster tissue. OsHV-1 was not detected in oysters at Oregon and Washington test sites. In Tomales Bay, California, high variation among replicate culture units was observed in cumulative field survival and peak viral load. A negative relationship was observed between peak OsHV-1 load in oyster tissues and shell height at the time of peak viral load, suggesting larger seed may be less vulnerable during periods of OsHV-1 infection risk. Cumulative survival over the duration of the growing season in Tomales Bay was related to peak viral load and differed by family. These results corroborate previous findings suggesting selective breeding may effectively increase survival of oyster families during OsHV-1 outbreaks along the US west coast.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"31-44"},"PeriodicalIF":1.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marteilia cocosarum in the Wash Estuary, UK: links to mass mortalities of cockles Cerastoderma edule and relationship to closely related species. 英国沃什河口的茧蚶：与贝壳类动物大量死亡的联系以及与近缘物种的关系。

IF 1.2 4区农林科学

Diseases of aquatic organisms Pub Date : 2025-10-09 DOI: 10.3354/dao03871

Anna Tidy, Ron Jessop, Georgia M Ward, Matthew J Green, Kelly S Bateman, David Bass, Jasmine E Hunt, Stuart H Ross, Chantelle Hooper

{"title":"Marteilia cocosarum in the Wash Estuary, UK: links to mass mortalities of cockles Cerastoderma edule and relationship to closely related species.","authors":"Anna Tidy, Ron Jessop, Georgia M Ward, Matthew J Green, Kelly S Bateman, David Bass, Jasmine E Hunt, Stuart H Ross, Chantelle Hooper","doi":"10.3354/dao03871","DOIUrl":"https://doi.org/10.3354/dao03871","url":null,"abstract":"Globally, Marteilia spp. parasites have been associated with significant mass mortality events in populations of commercially important bivalve molluscs, frequently resulting in large-scale fishery collapses and substantial socio-economic impacts. The Wash Estuary, UK, supports several bivalve fisheries, and among these, common cockles Cerastoderma edule have suffered unusually high mortalities since 2008. We investigate potential causes of these mortalities, and confirm infection with M. cocosarum, strongly associated with cockle moribundity, also confirming its presence in archived samples collected in 2009. Molecular and light microscopy screening of samples collected during mortality events in 2021, including healthy (buried) and moribund (weak, unable to bury) cockles, indicated high prevalence of M. cocosarum in moribund cockles (PCR incidence up to 95%) in contrast to healthy cockles (up to 42%), suggesting an association between cockle moribundity and Marteilia infection. Analysis of the full ribosomal RNA array identified consistently different nucleotides between M. cocosarum infections in the Wash (denoted as genotype WE1) and those in Wales (denoted genotype WA1). A total of 83% of infections in the Wash could be identified as M. cocosarum WE1 and 12% as M. cocosarum WA1, with both genotypes recovered from 5% of infected animals. Histopathologically, M. cocosarum WE1 infects the gill, mantle and connective tissues, identical to observations of M. cocosarum infecting Welsh cockles. Ongoing cockle mortalities in the Wash raise concerns regarding the sustainability of this resource ecologically and economically. Additional measures may be required to reduce the spread of this pathogen, noting that its distribution beyond the Wash and Wales is currently unknown.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"164 ","pages":"13-30"},"PeriodicalIF":1.2,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145250308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0