Shewanella putrefaciens as an emerging pathogen of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis.

IF 1.1 4区农林科学 Q3 FISHERIES

Diseases of aquatic organisms Pub Date : 2024-08-29 DOI:10.3354/dao03811

Jie Liu, Chenhao Teng, Xurui Zheng, La Xu, Haipeng Cao, Chunlei Gai

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引用次数: 0

Abstract

Shewanella putrefaciens has been recognized as an emerging important pathogen in aquaculture. However, scarce information is available on the characterization and microbial control of S. putrefaciens as a causal agent of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. In this study, a multi-resistant S. putrefaciens isolate (DZ-A) was identified as a causal pathogen of hepatopancreas necrosis disease in Chinese mitten crabs. It showed a lethal dose (LD50) of 2.20 × 105 CFU ml-1 in Chinese mitten crabs, and multiple resistance to aminoglycoside, chloramphenicol, macrolide, penicillin, peptide, and tetracycline antimicrobials. In addition, Bdellovibrio powder exhibited a significant antibacterial effect against the pathogenic S. putrefaciens, and conferred significant protection to challenged Chinese mitten crabs with relative percentage survivals of 80.00% to 93.33% via significant improvement in their immune response and antioxidant capability. The findings of this study provide valuable insights into the phenotypic characterization and biological control of pathogenic S. putrefaciens in Chinese mitten crabs.

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中华绒螯蟹肝胰腺坏死病的新病原体--普氏雪旺菌。

腐生雪旺氏菌已被认为是水产养殖中一种新出现的重要病原体。然而，有关中华绒螯蟹肝胰腺坏死病病原S. putrefaciens的特征和微生物控制的信息却很少。在这项研究中，一个多重耐药的中华绒螯蟹分离株（DZ-A）被确定为中华绒螯蟹肝胰腺坏死病的病原体。它对中华绒螯蟹的致死剂量（LD50）为 2.20 × 105 CFU ml-1，并对氨基糖苷类、氯霉素、大环内酯类、青霉素、肽类和四环素类抗菌药具有多重耐药性。此外，Bdellovibrio 菌粉对致病性 S. putrefaciens 有显著的抗菌作用，并通过显著改善中华绒螯蟹的免疫反应和抗氧化能力，为中华绒螯蟹提供显著的保护，相对存活率为 80.00% 至 93.33%。这项研究的结果为中华绒螯蟹致病性 S. putrefaciens 的表型特征和生物防治提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diseases of aquatic organisms 农林科学-兽医学

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

8-16 weeks

期刊介绍： DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis