Diseases of aquatic organisms最新文献_第6页

Association of ectoparasite Lepeophtheirus salmonis counts on farmed Atlantic salmon and wild sea trout in Scotland. 苏格兰养殖大西洋鲑鱼和野生海鳟体外寄生虫 Lepeophtheirus salmonis 数量的关联。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03774

Stephen C Ives, Alexander G Murray, John D Armstrong

{"title":"Association of ectoparasite Lepeophtheirus salmonis counts on farmed Atlantic salmon and wild sea trout in Scotland.","authors":"Stephen C Ives, Alexander G Murray, John D Armstrong","doi":"10.3354/dao03774","DOIUrl":"10.3354/dao03774","url":null,"abstract":"Parasitic sea lice (Copepoda: Caligidae) colonising marine salmonid (Salmoniformes: Salmonidae) aquaculture production facilities have been implicated as a possible pressure on wild salmon and sea trout populations. This investigation uses monitoring data from the mainland west coast and Western Isles of Scotland to estimate the association of the abundance of adult female Lepeophtheirus salmonis (Krøyer) colonising farmed Atlantic salmon Salmo salar L. with the occurrence of juvenile and mobile L. salmonis on wild sea trout, anadromous S. trutta L. The associations were evaluated using generalised linear mixed models incorporating farmed adult female salmon louse abundances which are temporally lagged relative to dependent wild trout values. The pattern of lags, which is consistent with time for L. salmonis development between egg and infective stage, was evaluated using model deviances. A significant positive association is identified between adult female L. salmonis abundance on farms and juvenile L. salmonis on wild trout. This association is consistent with a causal relationship in which increases in the number of L. salmonis copepodids originating from lice colonising farmed Atlantic salmon cause an increase of L. salmonis abundance on wild sea trout.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"95-106"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc). 评估针对恙虫病（Diadema antillarum scuticociliatosis，DaSc）病原体的体外疗法。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03776

James S Evans, Julie J Voelschow, Isabella T Ritchie, Mya Breitbart, Ian Hewson, Christina A Kellogg

{"title":"Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc).","authors":"James S Evans, Julie J Voelschow, Isabella T Ritchie, Mya Breitbart, Ian Hewson, Christina A Kellogg","doi":"10.3354/dao03776","DOIUrl":"10.3354/dao03776","url":null,"abstract":"In the 1980s, a mass die-off of the long-spined sea urchin Diadema antillarum occurred on Florida and Caribbean coral reefs. D. antillarum populations largely did not recover, and in 2022, remaining populations experienced another mass mortality event. A ciliate most similar to Philaster apodigitiformis was identified as the causative agent of the 2022 event, which was named D. antillarum scuticociliatosis (DaSc). Here, we investigated possible treatments for this pathogen. We tested the efficacy of 10 compounds at final concentrations of 100, 50, 25, 12.5, 6.25, and 3.13 µM, or a 10-fold serial dilution series, against ciliates cultured from an infected D. antillarum specimen. Of the tested compounds, 8 induced 100% ciliate mortality at some dose after 24 h. The most effective (defined as those requiring the lowest dose to induce 100% ciliate mortality) were quinacrine and tomatine (both effective at 12.5 µM), followed by furaltadone and plumbagin (25 µM), bithionol sulfoxide and 2'4' dihydroxychalcone (50 µM), and oxyclozanide and carnidazole (100 µM). Toltrazuril and a commercially available anticiliate product containing naphthoquinones were not effective at any dose tested. Shortened (15 min) time trials were performed using ciliate cultures reared in natural seawater to better reflect natural environmental conditions, and revealed that 2 of the compounds (quinacrine and tomatine) induced 100% ciliate mortality at 100 µM, with tomatine also effective at 50 µM. This study identified several treatments effective against the causative agent of DaSc in vitro, but their toxicity and utility in vivo remain unknown.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"107-112"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First molecular confirmation of cyprinid herpesvirus 1 (CyHV1) in diseased carp in Serbia. 首次从分子角度证实塞尔维亚患病鲤鱼体内存在鲤科疱疹病毒 1 (CyHV1)。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03777

Vladimir Radosavljevic, Dimitrije Glisic, Jelena Maksimovic-Zoric, Ljubisa Veljovic, Bozidar Savic

引用次数: 0

Susceptibility of shellfish aquaculture species in the Chesapeake Bay and Maryland coastal bays to ostreid herpesvirus 1 microvariants. 切萨皮克湾和马里兰沿海海湾的贝类水产养殖物种对奥斯特里德疱疹病毒 1 微变种的易感性。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03775

M L Kachmar, K S Reece, M V Agnew, H J Schreier, C A Burge

{"title":"Susceptibility of shellfish aquaculture species in the Chesapeake Bay and Maryland coastal bays to ostreid herpesvirus 1 microvariants.","authors":"M L Kachmar, K S Reece, M V Agnew, H J Schreier, C A Burge","doi":"10.3354/dao03775","DOIUrl":"10.3354/dao03775","url":null,"abstract":"Ostreid herpesvirus 1 (OsHV-1) and its microvariants (µVars) cause economically devastating mass mortalities of oysters and pose a threat to the shellfish aquaculture industry globally. OsHV-1 outbreaks can cause up to 100% mortality in the Pacific oyster Crassostrea gigas. However, OsHV-1 and its variants have a broad host range and can infect at least 7 bivalve species, including bay scallops Argopecten irradians and eastern oysters C. virginica. Determining the susceptibility of economically and ecologically important bivalve species to OsHV-1 is critical for improving biosecurity and disease management to protect the aquaculture industry. Surveys of eastern oysters were conducted in June to August 2021 in the Maryland portion of the Chesapeake Bay to determine the prevalence and viral load of OsHV-1 at 5 aquaculture farms. Using quantitative PCR, OsHV-1 was not detected at any sites. Experiments examined the susceptibility of single stocks of eastern oysters and hard clams Mercenaria mercenaria to the virus and their ability to horizontally transmit it using OsHV-1 µVar SD (San Diego, California) and OsHV-1 µVar FRA (Marennes-Olreon, France). Results showed that OsHV-1 µVars did not cause mortality or symptomatic infection in the single stocks of eastern oysters and hard clams used in these experiments using natural infection pathways. However, the eastern oyster stock, when injected with OsHV-1, did transmit the virus to naïve Pacific oysters. Further experimentation using additional stocks and lines and establishment of surveillance programs along the east and Gulf coasts of the USA are necessary to prepare for the potential spread and impact of OsHV-1 related disease.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"113-127"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Habitat shift of adult Caligus undulatus (Copepoda: Siphonostomatoida: Caligidae) from host fish to plankton in response to host behavior. 桡足类：虹吸目：桡足科）成体根据宿主行为从宿主鱼类转移到浮游生物的栖息地。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03773

Yusuke Kondo, Susumu Ohtsuka, Masaki Nawata, Yusuke Nishida, Sota Komeda, Sadaharu Iwasaki, Panakkool Thamban Aneesh, Balu Alagar Venmathi Maran

{"title":"Habitat shift of adult Caligus undulatus (Copepoda: Siphonostomatoida: Caligidae) from host fish to plankton in response to host behavior.","authors":"Yusuke Kondo, Susumu Ohtsuka, Masaki Nawata, Yusuke Nishida, Sota Komeda, Sadaharu Iwasaki, Panakkool Thamban Aneesh, Balu Alagar Venmathi Maran","doi":"10.3354/dao03773","DOIUrl":"10.3354/dao03773","url":null,"abstract":"Before 2019, adults of the sea louse Caligus undulatus were reported exclusively in plankton from ocean samples worldwide and were not known to parasitize fish hosts. In 2019, the first instance of this caligid parasitizing a fish host, Japanese sardinella Sardinella zunasi, was reported in the Seto Inland Sea, Japan. The presently reported study aimed to investigate the biology and ecology of adult C. undulatus in plankton communities in the Seto Inland Sea and surrounding waters from March 2020 to November 2021. The occurrence of sea lice in plankton communities was restricted to the period of August-January, mainly between October and December with maximum plankton abundance (10.5 ind. per 1000 m3) recorded on 30 November 2020. All post-naupliar stages of C. undulatus were found on the host fish, and they represented a typical life cycle pattern known for Caligus species. The sex ratios in both planktonic and parasitic adults were not significantly different. The frequency of occurrence of planktonic and parasitic adult females with egg strings was 68 and 46%, respectively. The number of eggs per string was significantly higher in parasitic adult females (mean ± SD: 16.9 ± 8.6) than in planktonic females (10.4 ± 10.8). These data suggest that adult females were detached from their hosts and continued to produce eggs without feeding. Seasonal migration of S. zunasi to brackish water for spawning may result in the detachment of mature caligids from the host and may be effective in protecting the offspring, which are less tolerant of less brackish water.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"81-94"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel lambdapapillomavirus in northern sea otters Enhydra lutris kenyoni, associated with oral hyperplastic nodules. 与口腔增生性结节有关的北部海獭Enhydra lutris kenyoni体内的新型羊羔病毒。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-02-29 DOI: 10.3354/dao03771

Carlos H Romero, Pam Tuomi, Kathleen A Burek-Huntington, Verena A Gill

{"title":"Novel lambdapapillomavirus in northern sea otters Enhydra lutris kenyoni, associated with oral hyperplastic nodules.","authors":"Carlos H Romero, Pam Tuomi, Kathleen A Burek-Huntington, Verena A Gill","doi":"10.3354/dao03771","DOIUrl":"10.3354/dao03771","url":null,"abstract":"A novel papillomavirus (PV) associated with hyperplastic nodules scattered over the muco-cutaneous border of the oral cavity of a dead, wild, subadult northern sea otter Enhydra lutris kenyoni (NSO) in 2004 in Homer, Alaska, USA, was genetically characterized. Primers for the amplification of 2 large overlapping DNA fragments that contained the complete genome of the NSO PV were designed. Sanger methodology generated sequences from which new specific primers were designed for the primer-walking approach. The NSO PV genome consists of 8085 nucleotides and contains an early region composed of E6, E7, E1, and E2 open reading frames (ORFs), an E4 ORF (contained within E2) lacking an in-frame proximal ATG start codon, an unusually long (907 nucleotide) stretch lacking any ORFs, a late region that contains the capsid genes L2 and L1, and a non-coding regulatory region (ncRR). This NSO PV has been tentatively named Enhydra lutris kenyoni PV2 (ElkPV2). Pairwise and multiple sequence alignments of the complete L1 ORF nucleotides and concatenated E1-E2-L1 amino acid sequences showed that the NSO PV is a novel PV, phylogenetically most closely related to southern sea otter PV1. The carboxy end of the E6 oncoprotein does not contain the PDZ-binding motif with a strong correlation with oncogenicity, suggesting a low-risk PV, which is in agreement with histopathological findings. However, the ElkPV2 E7 oncoprotein does contain the retinoblastoma (pRb) binding domain LXCXE (LQCYE in ElkPV2), associated with oncogenicity in some high-risk PVs. Further studies on the prevalence and clinical significance of ElkPV2 infections in NSO are needed.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"73-80"},"PeriodicalIF":1.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in Paramarteilia canceri infections in velvet crab Necora puber. 绒蟹（Necora puber）体内癌副马氏管感染的变异。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-02-29 DOI: 10.3354/dao03772

Signe Martin, Deborah Cheslett, Evelyn Collins, Simona Georgieva, Ian O Connor, Fiona Swords, Katie O Dwyer

{"title":"Variation in Paramarteilia canceri infections in velvet crab Necora puber.","authors":"Signe Martin, Deborah Cheslett, Evelyn Collins, Simona Georgieva, Ian O Connor, Fiona Swords, Katie O Dwyer","doi":"10.3354/dao03772","DOIUrl":"10.3354/dao03772","url":null,"abstract":"Sustainable management of crustacean populations requires an understanding of the range of factors affecting different crustacean species. Recently, a high prevalence of a paramyxid parasite, Paramarteilia canceri, was reported in velvet crabs Necora puber in Ireland. Similar parasites have been known to cause mass mortalities in bivalves and, as velvet crabs are an important commercial species, these parasite infections are cause for concern. The main objective of this study was to examine variation in P. canceri infections in relation to host biology and season over a 2 yr period. In addition, we tested a range of host tissues and organs to gain more information on the host-parasite interaction. The parasite was present in all tissues and organs investigated, including the gonad and eggs of a berried female. Parasite prevalence was highest in the cuticular epithelium and hepatopancreas. Both annual and seasonal variation was found in parasite prevalence and parasite load. No difference was found in parasite prevalence or parasite load with either crab size or crab sex. Granulomas as a response to infection were significantly more abundant in infected velvet crab individuals. The results of this study provide important information on the host-parasite interaction between P. canceri and the velvet crab and highlight the importance of including parasite monitoring in the management of crustacean fisheries.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"61-72"},"PeriodicalIF":1.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erysipelas with preferential brain and skin involvement in a Mediterranean bottlenose dolphin Tursiops truncatus. 地中海瓶鼻海豚（Tursiops truncatus）患红斑性溃疡，脑部和皮肤优先受累。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-02-01 DOI: 10.3354/dao03770

Laura Martino, Bárbara Serrano, Jaume Alomar, Lola Pérez, Virginia Aragon, Alex Cobos, Maria Lourdes Abarca, Zeinab Yazdi, Esteban Soto, Mariano Domingo

{"title":"Erysipelas with preferential brain and skin involvement in a Mediterranean bottlenose dolphin Tursiops truncatus.","authors":"Laura Martino, Bárbara Serrano, Jaume Alomar, Lola Pérez, Virginia Aragon, Alex Cobos, Maria Lourdes Abarca, Zeinab Yazdi, Esteban Soto, Mariano Domingo","doi":"10.3354/dao03770","DOIUrl":"10.3354/dao03770","url":null,"abstract":"Infections by Erysipelothrix rhusiopathiae occur in domestic animals and cause the disease known as 'erysipelas'. The ubiquity of Erysipelothrix spp. makes infection possible in a wide range of vertebrates and invertebrates. Cetaceans are highly susceptible to erysipelas, especially those under human care. The number of cases documented in wild cetaceans is low, the pathogenesis is incompletely understood, and the full spectrum of lesions is not well defined. The possible serotypes and species of the genus that can cause disease are unknown. In October 2022, a common bottlenose dolphin Tursiops truncatus stranded in Vilassar de Mar (Catalonia) showing skin lesions consistent with 'diamond skin disease', a characteristic lesion of erysipelas shared by swine and cetaceans. Necropsy was performed following standardized procedures, and multiple samples were taken for histopathology and bacteriology. Erysipelothrix sp. grew in pure culture in many tissue samples. Genetic characterization by multi-locus sequence analysis identified the species as E. rhusiopathiae. Histologically, the main lesions were an intense suppurative vasculitis of leptomeningeal arteries and veins with abundant intramural Gram-positive bacilli and meningeal hemorrhages. Meningeal lesions were considered the cause of death. The affected skin showed moderate suppurative dermatitis. Herein we document a case of erysipelas in a Mediterranean common bottlenose dolphin with unusual lesions in the leptomeningeal vessels and marked skin tropism. To our knowledge, this is the first case of severe brain involvement in erysipelas in a cetacean. We also provide a review of available cases in wild cetaceans, to highlight the characteristics of the disease and improve future diagnosis.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"31-43"},"PeriodicalIF":1.4,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139650399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diet-induced nephrocalcinosis in aquarium-raised juvenile spotted wolffish Anarhichas minor. 水族馆饲养的斑狼鱼幼鱼饮食引起的肾钙化症。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-01-18 DOI: 10.3354/dao03769

Karine Béland, Catherine Rousseau, Stéphane Lair

{"title":"Diet-induced nephrocalcinosis in aquarium-raised juvenile spotted wolffish Anarhichas minor.","authors":"Karine Béland, Catherine Rousseau, Stéphane Lair","doi":"10.3354/dao03769","DOIUrl":"10.3354/dao03769","url":null,"abstract":"Wolffish are regularly housed in aquaria, but little data on their husbandry and health is available for caretakers. High occurrence rates of nephrocalcinosis and urolithiasis have been observed in Atlantic Anarhichas lupus and spotted A. minor wolffish housed at 2 Canadian zoological institutions. To explore the effect of diet on nephrocalcinosis and urolithiasis development, a 16 mo prospective study was conducted. A total of 32 juvenile spotted wolffish were randomly assigned to one of 4 experimental groups fed exclusively with the following diet: (1) Skretting® Europa 18 pellets; (2) Mazuri® LS Aquatic Carni-Blend Diet Formula; (3) vitamin-supplemented fish-based diet, and (4) vitamin-supplemented invertebrate-based diet. Urinalysis, radiographs, and complete necropsies were performed at the end of the study. None of the wolffish developed uroliths during the study period. All specimens fed with the fish-based and invertebrate-based diets developed nephrocalcinosis, whereas this condition was seen in 12.5 and 0% of the fish in the Skretting® and Mazuri® groups, respectively. Affected wolffish often presented with oxalate crystalluria and increased radiodensity of the posterior kidneys. Urinalysis and radiographic study were considered useful in the antemortem diagnosis of nephrocalcinosis. None of the previously published risk factors for the development of nephrocalcinosis in fish were supported by the results of this study. However, nutritional analyses of the 4 diets suggest that high dietary levels of gelatin or vitamin C or low levels of vitamin E could be potential risk factors for the development of nephrocalcinosis in spotted wolffish and thus warrant further study.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"19-30"},"PeriodicalIF":1.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brome mosaic virus (BMV) capsid protein virus-like particle-delivering dsRNA targeting VP28 gene of white spot syndrome virus (WSSV) through feed reduced Penaeus vannamei shrimp mortality from WSSV infection 通过饲料传递针对白斑综合征病毒(WSSV) VP28基因的衣壳蛋白病毒样颗粒可降低凡纳滨对虾对白斑综合征病毒(WSSV)感染的死亡率

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2023-10-26 DOI: 10.3354/dao03754

EA Ruiz Guerrero, I. Giffard Mena, M. Viana, S. Ramos Carreño, S. Sánchez Serrano

{"title":"Brome mosaic virus (BMV) capsid protein virus-like particle-delivering dsRNA targeting VP28 gene of white spot syndrome virus (WSSV) through feed reduced Penaeus vannamei shrimp mortality from WSSV infection","authors":"EA Ruiz Guerrero, I. Giffard Mena, M. Viana, S. Ramos Carreño, S. Sánchez Serrano","doi":"10.3354/dao03754","DOIUrl":"https://doi.org/10.3354/dao03754","url":null,"abstract":"Numerous strategies have been investigated to combat viral infections in shrimp, specifically targeting the white spot syndrome virus (WSSV) that has caused outbreaks worldwide since the 1990s. One effective treatment involves intramuscular application of dsRNA-mediated interference against the viral capsid protein VP28. However, this approach presents challenges in terms of individual shrimp management, limiting its application on a large scale. To address this, our study aimed to evaluate the efficacy of oral delivery of protected dsRNA using chitosan nanoparticles or virus-like particles (VLPs) synthesized in brome mosaic virus (BMV). These delivery systems were administered before, during, and after WSSV infection to assess their therapeutic potential. Our findings indicate that BMV-derived VLPs demonstrated superior efficiency as nanocontainers for dsRNA delivery. Notably, the treatment involving vp28 dsRNA mixed in the feed and administered simultaneously to shrimp already infected with WSSV exhibited the highest survival rate (48%), while the infected group had a survival rate of zero, suggesting the potential efficacy of this prophylactic approach in commercial shrimp farms.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"1 1","pages":"15-28"},"PeriodicalIF":1.4,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69648134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0