DNA sequence : the journal of DNA sequencing and mapping最新文献_第7页

Cloning and characterization of Xenopus dicalcin, a novel S100-like calcium-binding protein in Xenopus eggs. 爪蟾卵中一种新的s100样钙结合蛋白——爪蟾钙蛋白的克隆与特性研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701241470

Naofumi Miwa, Yukiko Shinmyo, Satoru Kawamura

引用次数: 8

Novel mutations in the SLC12A3 gene causing Gitelman's syndrome in Swedes. SLC12A3基因的新突变导致瑞典人的吉特曼综合征。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701400456

Cristiano Fava, Martina Montagnana, Lena Rosberg, Philippe Burri, Anders Jönsson, Pär Wanby, Hans Wahrenberg, U Lennart Hulthén, Mattias Aurell, Gian Cesare Guidi, Olle Melander

{"title":"Novel mutations in the SLC12A3 gene causing Gitelman's syndrome in Swedes.","authors":"Cristiano Fava, Martina Montagnana, Lena Rosberg, Philippe Burri, Anders Jönsson, Pär Wanby, Hans Wahrenberg, U Lennart Hulthén, Mattias Aurell, Gian Cesare Guidi, Olle Melander","doi":"10.1080/10425170701400456","DOIUrl":"https://doi.org/10.1080/10425170701400456","url":null,"abstract":"Purpose: Gitelman's syndrome (GS) is an inherited autosomal recessive disorder due to loss of function mutations in the SLC12A3 gene encoding the Na-Cl co-transporter (NCCT), the target of thiazide diuretics. The defective function of the NCCT, which normally is expressed in the apical membrane of the distal convolute tubule in the kidney, leads to mild hypotension, hypokalemia, hyperreninemic hyperaldosteronism, mild metabolic alkalosis, hypomagnesemia and hypocalciuria. Up to now, more than 100 mutations of the SLC12A3 gene have been described in GS patients.Methods: We have collected 30 patients from Sweden with a clinical diagnosis of GS and undertaken a mutation screening by SSCP and successive sequencing of the 26 exons and intronic boundaries. Both mutations were identified in most (n = 28, 93%) and at least one mutation was identified in all patients.Results: We found 22 different mutations evenly distributed throughout the gene, 11 of which have not been described previously. The new variants include 8 missense mutations (Glu68Lys, His69Asn, Argl45His, Vall53Met, Gly230Asp, Gly342Ala, Val677Leu and Gly867Ser), 1 insertion (c.834_835insG on exon 6) and 2 splice-site mutations (c.2667 + lT>G substitution in splicing donor site after exon 22, c.1569-1G>A substitution in the splicing acceptor site before exon 13).Conclusion: In Swedish patients with the clinical features of GS, disease-causing mutations in the SLC12A3 gene were identified in most patients. The spectrum of GS mutations is wide making full mutation screening of the SLC12A3 gene necessary to confirm the diagnosis.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"395-9"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701400456","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Isolation and characterization of the ATP-binding cassette (ABC) transporter system genes from loofah witches' broom phytoplasma. 丝瓜丛枝植物原体中atp结合盒(ABC)转运体系统基因的分离与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701350784

Chun-Lin Huang, Kuo-Chieh Ho

{"title":"Isolation and characterization of the ATP-binding cassette (ABC) transporter system genes from loofah witches' broom phytoplasma.","authors":"Chun-Lin Huang, Kuo-Chieh Ho","doi":"10.1080/10425170701350784","DOIUrl":"https://doi.org/10.1080/10425170701350784","url":null,"abstract":"A clone containing a 3903 bp EcoRI-restriction fragment was obtained from a lambda(ZAP) genomic library of loofah witches' broom (LfWB) phytoplasma by plaque hybridization using a PCR fragment as a probe. Sequence analysis revealed that this fragment contained three open reading frames (ORFs). The deduced amino acid sequences of ORF 1 and ORF 2 showed a high homology with the ATP-binding proteins of the ABC transporter system genes of prokaryotes and eukaryotes, and encoded proteins with a molecular mass of 36 and 30 kDa, respectively. Based on amino acid sequence similarity, secondary structure, hydrophilicity and a signal peptide sequence at the N-terminus, we predicted that ORF 3 might encode a specific solute-binding prolipoprotein of the ABC transporter system with a molecular mass of 62 kDa. The cleavage site of this prolipoprotein signal peptide was similar to those of gram-positive bacteria. In addition to nutrient uptake, ABC transporter systems of bacteria also play a role in signal transduction, drug-resistance and perhaps virulence. The possible implications of the system to the survival and the pathogenesis of phytoplasma were discussed.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"347-56"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701350784","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Single nucleotide polymorphisms in the open reading frame of the stearoyl-CoA desaturase gene and resulting genetic variants in Canadian Holstein and Jersey cows. 加拿大荷斯坦奶牛和泽西奶牛硬脂酰辅酶a去饱和酶基因开放阅读框中的单核苷酸多态性及由此产生的遗传变异。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701291921

Patrick M Kgwatalala, Eveline M Ibeagha-Awemu, John F Hayes, Xin Zhao

{"title":"Single nucleotide polymorphisms in the open reading frame of the stearoyl-CoA desaturase gene and resulting genetic variants in Canadian Holstein and Jersey cows.","authors":"Patrick M Kgwatalala, Eveline M Ibeagha-Awemu, John F Hayes, Xin Zhao","doi":"10.1080/10425170701291921","DOIUrl":"https://doi.org/10.1080/10425170701291921","url":null,"abstract":"Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of conjugated linoleic acid (CLA) and mono-unsaturated fatty acids (MUFA) from their saturated counterparts in the mammary gland and adipose tissue of ruminant animals. We hypothesize that single nucleotide polymorphisms (SNPs) in the SCD gene account for some of the differences in SCD activity, and consequently for some of the variations in CLA and MUFA content of milk fat between Holsteins and Jersey cows and within these two breeds. We analyzed the open reading frame of the SCD gene of 44 Holsteins and 48 Jerseys for SNPs by sequencing. Three SNPs: 702A --> G, 762T --> C and 878C --> T were identified in both breeds and a further SNP, 435G --> A, was unique to Holsteins. The SNPs characterized four different genetic variants in Holsteins: A (G(435)A(702)T(762)C(878)), A1 (A(435)A(702)T(762)C(878)), B (G(435)G(702)C(762)T(878)) and B1 (A(435)G(702)C(762)T(878)), with only variants A and B in Jerseys. SNP 878C --> T resulted in a non-synonymous codon change while the rest resulted in synonymous codon changes giving rise to two protein variants, A having alanine and B having valine. Allele A was the most prevalent in the two breeds. These differences may, therefore, contribute to existing variations in CLA and fat content between and within Canadian Holstein and Jersey cows.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"357-62"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701291921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Cloning, sequence analysis and phylogeny of connexin43 isolated from American black bear heart. 美洲黑熊心脏connexin43基因的克隆、序列分析及系统发育。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701400589

Marcel A G Van Der Heyden, Bart Kok, Evelyn N Kouwenhoven, Øivind Tøien, Brian M Barnes, Vadim G Fedorov, Igor R Efimov, Tobias Opthof

{"title":"Cloning, sequence analysis and phylogeny of connexin43 isolated from American black bear heart.","authors":"Marcel A G Van Der Heyden, Bart Kok, Evelyn N Kouwenhoven, Øivind Tøien, Brian M Barnes, Vadim G Fedorov, Igor R Efimov, Tobias Opthof","doi":"10.1080/10425170701400589","DOIUrl":"https://doi.org/10.1080/10425170701400589","url":null,"abstract":"Conduction in the heart requires gap junctions. In mammalian ventricular myocytes these consist of connexin43 (Cx43). Hearts of non-hibernating species display conduction disturbances at reduced temperatures. These may exacerbate into lethal arrhythmias. Hibernating species are protected against these arrhythmias by a non-resolved mechanism. To analyze whether the amino acid composition of Cx43 from the hibernating American black bear displays specific features, we cloned the full coding sequence of Ursus americanus Cx43 and compared with that of other (non)hibernating species. UaCx43 displays 99.7% identity to rabbit Cx43 at the amino acid level. No specific features were observed in UaCx43 when compared to previously cloned Cx43 from hibernating and non-hibernating mammals. Phylogenetic tree reconstruction of this and other published full-length Cx43 sequences reveals a very high level of conservation from fish to men. Finally, one of the previously identified six mammalian characteristic amino acids, is not conserved in the black bear.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"380-4"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701400589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Statement of retraction. 撤回声明。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01

引用次数: 0

Genomic organization and polymorphisms detected by denaturing high-performance liquid chromatography of porcine SLC11A1 gene. 变性高效液相色谱法检测猪SLC11A1基因的基因组组织及多态性。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170600857541

Zhen-Fang Wu, Wen-Hua Luo, Guan-Fu Yang, Xi-Quan Zhang

{"title":"Genomic organization and polymorphisms detected by denaturing high-performance liquid chromatography of porcine SLC11A1 gene.","authors":"Zhen-Fang Wu, Wen-Hua Luo, Guan-Fu Yang, Xi-Quan Zhang","doi":"10.1080/10425170600857541","DOIUrl":"https://doi.org/10.1080/10425170600857541","url":null,"abstract":"SLC11A1 (also known as Natural Resistance Associated Macrophage Protein1, NRAMP1) plays a crucial role in resistance of inbred mice to infection with several intracellular pathogens such as Mycobacterium, Leishmania and Salmonella. In this study, PCR amplification and sequencing were performed to obtain the genomic organization and sequence of porcine SLC11A1 gene by comparative genomic analysis. Results showed that porcine SLC11A1 gene consists of 15 exons and 14 introns, which is consistent with that of mice and human. All introns were sequenced and their nucleotide sequences were submitted to GenBank. The exon/intron boundaries were determined by comparing cDNA sequence with amplified genomic DNA sequences. Mutational analysis was performed on exonic and neighboring intronic region by denaturing high-performance liquid chromatography (DHPLC) and sequencing confirmation. Forty polymorphisms were identified; six are located in exons and thirty-four in introns. Two exonic polymorphisms are nonsynonymous changes (D6H and V175I), three are synonymous changes (S23, G33 and I155), and one is in 3' UTR. The availability of the fine genomic organization and identification of the polymorphisms will facilitate the evaluation of porcine SLC11A1 functional role in diseases resistance or susceptibility.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"327-33"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170600857541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Molecular cloning and characterization of buffalo alpha(s1)-casein gene. 水牛α (s1)-酪蛋白基因的克隆与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701388974

S Sukla, T K Bhattacharya, R T Venkatachalapathy, P Kumar, A Sharma

{"title":"Molecular cloning and characterization of buffalo alpha(s1)-casein gene.","authors":"S Sukla, T K Bhattacharya, R T Venkatachalapathy, P Kumar, A Sharma","doi":"10.1080/10425170701388974","DOIUrl":"https://doi.org/10.1080/10425170701388974","url":null,"abstract":"Buffaloes in Indian subcontinent play an important role as the producer of milk and milk products. The alpha(s1)-casein constitutes 38% of the total milk proteins. The present study was carried out to characterize the gene in Murrah breed of Riverine buffalo. Buffalo alpha(s1)-casein cDNA was synthesized by RT-PCR, then cloned using pDRIVE-cloning vector and sequenced. The sequencing revealed that the size of alpha(s1)-casein cDNA was of 645 bp with GC content of 45.58%. The alpha(s1)-casein gene coded 214 amino acids precursor with a signal peptide of 15 amino acid residues. The similarity of buffalo alpha(s1)-casein mRNA sequence with that of cattle, goat, sheep, pig, camel, equine and human were estimated as 97.2, 93, 92.3, 57.2, 59.5, 55.9 and 46.6%, respectively. A similar trend was observed when compared amino acid sequences of these species. In the phylogenetic trees, constructed from the data of the alpha(s1)-casein mRNA as well as protein sequences, it has been observed that buffalo, cattle, goat and sheep formed a cluster with a closer relationship between cattle and buffalo followed by goat and sheep.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"334-40"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701388974","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Molecular cloning and characterization of a glutathione S-transferase gene from Ginkgo biloba. 银杏谷胱甘肽s -转移酶基因的克隆与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701389063

Xuefen Liu, Zhongxiang Deng, Shi Gao, Xiaofen Sun, Kexuan Tang

{"title":"Molecular cloning and characterization of a glutathione S-transferase gene from Ginkgo biloba.","authors":"Xuefen Liu, Zhongxiang Deng, Shi Gao, Xiaofen Sun, Kexuan Tang","doi":"10.1080/10425170701389063","DOIUrl":"https://doi.org/10.1080/10425170701389063","url":null,"abstract":"Glutathione S-transferases (GSTs) play an important role in the response of plants to changing environmental conditions. Here, we report the cloning of the GST gene for GST from Ginkgo biloba, a native medicinal plant species in China, by rapid amplification of cDNA ends (RACE). The full-length cDNA (designated as GbGST) was 1008 bp and contained a 684 bp open reading frame (ORF) encoding a polypeptide of 228 amino acids. The genomic sequence of GbGST was also obtained. Semi-quantitative RT-PCR analysis revealed that GbGST expressed in all tested tissues of G. biloba, including leaf, root and stem and the expression of GbGST could be induced by UV, MJ and drought treatments, suggesting that GbGST was potentially involved in plant's stress tolerance. To our knowledge, this is the first GST cDNA cloned from Ginkgoaceae. Based on comparative analyses of amino acid sequence, phylogeny, predicted three-dimensional structure together with the gene structure, the GbGST should be classified into the tau class.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"371-9"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701389063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The complete nucleotide sequence of Chinese hamster (Cricetulus griseus) mitochondrial DNA. 中国仓鼠(Cricetulus griseus)线粒体DNA全核苷酸序列。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170601101287

Michael A Partridge, Mercy M Davidson, Tom K Hei

引用次数: 34