DNA sequence : the journal of DNA sequencing and mapping最新文献_第8页

Evidence for maternal inheritance of mitochondrial DNA in allotetraploid. 异源四倍体线粒体DNA母系遗传的证据。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701248541

Xinhong Guo, Shaojun Liu, Yun Liu

{"title":"Evidence for maternal inheritance of mitochondrial DNA in allotetraploid.","authors":"Xinhong Guo, Shaojun Liu, Yun Liu","doi":"10.1080/10425170701248541","DOIUrl":"https://doi.org/10.1080/10425170701248541","url":null,"abstract":"The complete mitochondrial DNA (mtDNA) sequences of the allotetraploid and red crucian carp were determined in this paper. We compared the complete mtDNA sequences between the allotetraploid and its female parent red crucian carp, and between the allotetraploid and its male parent common carp. The results indicated that the complete mtDNA nucleotide identity (99.7%) between the allotetraploid and its female parent red crucian carp was higher than that (89.0%) between the allotetraploid and its male parent common carp. Moreover, the analysis on the start and stop codons, overlaps and spacers, and phylogeny of the mt genomes indicated the genetic relationship between the allotetraploid and its female parent red crucian carp was closer than that between the allotetraploid and its male parent common carp. Our results indicated that the allotetraploid mt genome was strictly maternally inherited. Through maternal inheritance, the mt genome in the F(11) allotetraploid displayed extremely high similarity to that in the female parent red crucian carp after 11 generations (from F(1) to F(11) hybrids). Such results indicated that the F(11) allotetraploid possessed the stable inheritance characteristic. Thus the tetraploid stocks possessed the good base to form a new tetraploid species in the future. Since the establishment of the new tetraploid stocks has the great significance in analyzing evolutionary theory of vertebrate and in improving aquaculture industry, analysis of the mt genome and the elucidation of the variation of the mt genome in the allotetraploid and its parents proved that it was a useful genetic marker to monitor the variations in the progeny of the crosses.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 4","pages":"247-56"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701248541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26753910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Complete mitochondrial genome of the rabbitfish Siganus fuscescens (Perciformes, Siganidae). 兔鱼的线粒体全基因组(鲈形目，兔鱼科)。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701248525

Dae-Ju Oh, Ji-Young Kim, Jung-A Lee, Weon-Jong Yoon, Soo-Yeong Park, Yong-Hwan Jung

{"title":"Complete mitochondrial genome of the rabbitfish Siganus fuscescens (Perciformes, Siganidae).","authors":"Dae-Ju Oh, Ji-Young Kim, Jung-A Lee, Weon-Jong Yoon, Soo-Yeong Park, Yong-Hwan Jung","doi":"10.1080/10425170701248525","DOIUrl":"https://doi.org/10.1080/10425170701248525","url":null,"abstract":"We determined the complete nucleotide sequence of the mitochondrial genome for the rabbitfish Siganus fuscescens (Perciformes, Siganidae). This mitochondrial genome, consisting of 16,491 base pairs (bp), included 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a noncoding control region similar those found in other vertebrates; the gene order was identical to that of typical vertebrates. Most of the genes of S. fuscescens were encoded on the H-strand, while the ND6 and eight tRNA (Gln, Ala, Asn, Cys, Tyr, Ser [UCN], Glu, and Pro) genes were encoded on the L-strand. The reading frames of ATPase 8 and 6 and those of ND4L and ND4 overlapped by ten and seven nucleotides, respectively. All mitochondrial protein-coding genes began with an ATG start codon, except for CO1, which started with GTG. Open reading frames of S. fuscescens ended with TAA (ND1, CO1, ATPase 8, ND4L, ND5 and ND6), and the remainder had incomplete stop codons, either TA (ATPase 6 and CO3) or T (ND2, CO2, ND3, ND4, and Cytb). The origin of L-strand replication in S. fuscescens was located in a cluster of five tRNA genes (WANCY) and was 34 nucleotides in length. A major noncoding region between the tRNA-Pro and tRNA-Phe genes (828 bp) was considered to be the control region (D-loop). Within this sequence, we identified a conserved sequence block characteristic of this region. The rabbitfish was grouped with Siganus canaliculatus in most parsimony analyses, which showed 100% bootstrap support for their divergence. These findings are useful for inferring phylogenetic relationships and identification within the suborder Acanthuroidei.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 4","pages":"295-301"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701248525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26754419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Molecular characterization, expression and mapping of porcine LMP2 and MECL-1 genes. 猪LMP2和MECL-1基因的分子特征、表达和定位。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170601119222

Yang Liu, He-Jun Li, Xiao-Tian Qiu, Hong-Wei Guo, Yu-Hua Li, Qin Zhang

{"title":"Molecular characterization, expression and mapping of porcine LMP2 and MECL-1 genes.","authors":"Yang Liu, He-Jun Li, Xiao-Tian Qiu, Hong-Wei Guo, Yu-Hua Li, Qin Zhang","doi":"10.1080/10425170601119222","DOIUrl":"https://doi.org/10.1080/10425170601119222","url":null,"abstract":"Low molecular mass polypeptides 2 (LMP2) and Multicatalytic endopeptidase complex subunit (MECL-1) are two of three catalytic beta-type subunits of the 20s proteasome and upon interferon gamma-induction. LMP2 is critical for the production of major histocompatibility complex (MHC) class I ligand and T-lymphocytes. The LMP2 gene is located in the MHC region, but MECL-1 is located outside the MHC region. They are involved in the antigen presentation and are important candidate genes for an initial exploration of relationships between the antigen processing genes and disease resistance. In this report, the porcine LMP2 and MECL-1 cDNA were cloned and A 5099 bp LMP2 genomic DNA structure was identified, then two single nucleotide polymorphisms were detected in the exon2 and exon5 of LMP2 gene in 367 individuals. The LMP2 and MECL-1 genes putative protein included 219,274 amino acids, respectively. Alignment and phylogenetic of predicted porcine LMP2 and MECL-1 amino acid sequence with their homologies were analyzed. Tissues expression of LMP2 and MECL-1 mRNA were observed by real time quantitative PCR (Q-PCR) method, the results revealed MECL-1 expressed widely in all tissues, but LMP2 was not detected in muscle. The porcine MECL-1 gene was mapped to chromosome 6, closely linked to microsatellite SW1108 (LOD = 4.09, 84cR) by radiation hybrid panel.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 4","pages":"257-64"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601119222","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26753911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Molecular cloning and sequence analysis of lamB encoding outer membrane maltose-inducible porin of Aeromonas hydrophila. 嗜水气单胞菌外膜麦芽糖诱导孔蛋白lamB基因的克隆及序列分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701248608

Tanuja Upadhyaya, Rajesh K Singh, Aparna Dixit

引用次数: 4

Molecular cloning and expression pattern of myostatin gene in yellow catfish (Pelteobagrus fulvidraco). 黄颡鱼肌肉生长抑制素基因的克隆及表达模式研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701243492

Jianlin Pan, Xiaolin Wang, Wei Song, Jianxiu Chen, Caojun Li, Qingshun Zhao

{"title":"Molecular cloning and expression pattern of myostatin gene in yellow catfish (Pelteobagrus fulvidraco).","authors":"Jianlin Pan, Xiaolin Wang, Wei Song, Jianxiu Chen, Caojun Li, Qingshun Zhao","doi":"10.1080/10425170701243492","DOIUrl":"https://doi.org/10.1080/10425170701243492","url":null,"abstract":"Myostatin (Mstn), a member of transforming growth factor beta (TGF-beta) superfamily, plays crucial roles in negative regulation of muscle growth. Yellow catfish, Pelteobagrus fulvidraco Richardson, is one of the most important freshwater aquaculture species in China, but little is known about its genes relate to growth. Here we report molecular cloning and expression pattern of Mstn gene in yellow catfish. Our results reveal that yellow catfish Mstn comprises three exons encoding a protein of 393 amino acid residues. Protein sequence alignments show that the Mstn exhibits 94% amino acid identity with other catfish Mstn and 59.3% identity with cattle Mstn, respectively. Moreover, the predicted bioactive form of yellow catfish Mstn shares 100% identity with other catfish and 87.1% identity with cattle Mstn respectively. Employing reverse transcription polymerase chain reaction (RT-PCR) analysis, we demonstrated that the yellow catfish Mstn gene is expressed in a variety of tissues with varied levels.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 4","pages":"279-87"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701243492","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26754417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Molecular cloning and characterization of Rab6 gene in duck. 鸭Rab6基因的克隆与特性分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701248509

Biao Dong, Daoqing Gong, Zhiliang Gu, He Meng

引用次数: 0

Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. 玫瑰花耐多药型ABC转运基因Crmdr1的克隆与特性研究

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701207018

Hongbin Jin, Donghui Liu, Kaijing Zuo, Yifu Gong, Zhiqi Miao, Yuhui Chen, Weiwei Ren, Xiaofen Sun, Kexuan Tang

{"title":"Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus.","authors":"Hongbin Jin, Donghui Liu, Kaijing Zuo, Yifu Gong, Zhiqi Miao, Yuhui Chen, Weiwei Ren, Xiaofen Sun, Kexuan Tang","doi":"10.1080/10425170701207018","DOIUrl":"https://doi.org/10.1080/10425170701207018","url":null,"abstract":"A novel gene encoding a MDR-like ABC transporter protein was cloned from Catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cDNA ends (RACE). This gene (named as Crmdr1; GenBank accession no.: DQ660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kDa. The CrMDR1 protein shared 59.8, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAD31576), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Crmdr1 was a low-copy gene. Expression pattern analysis revealed that Crmdr1 constitutively expressed in the root, stem and leaf, but with lower expression in leaf. The domains analysis showed that CrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in \"TMD1-NBD1-TMD2-NBD2\" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, \"Walker A\", \"Walker B\" and C motif, were found. These results indicate that CrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 4","pages":"316-25"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701207018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26754291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The cloning and sequencing of the UDP-galactose 4-epimerase gene (galE) from Avibacterium paragallinarum. 副翼鸟芽胞杆菌(Avibacterium paragallinarum) udp -半乳糖4- epimase基因(galE)的克隆与测序。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170701207067

Yolande Roodt, Robert Bragg, Jacobus Albertyn

引用次数: 4

Comprehensive analysis of prokaryotic mechanosensation genes: their characteristics in codon usage. 原核生物机械感觉基因的综合分析:密码子使用特征。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170601136564

Rong Chen, Hong Yan, Kong-Nan Zhao, Boris Martinac, Guang B Liu

引用次数: 8

Unusual organization associated to a tandem of IS231 may yield two peculiar cloverleaf secondary structures. 与IS231串联相关的不寻常组织可能产生两个特殊的三叶草二级结构。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-08-01 DOI: 10.1080/10425170601141127

Dong Xu, Jean-Charles Côté

引用次数: 0