DNA sequence : the journal of DNA sequencing and mapping最新文献_第10页

Sequencing of cDNA and proximal promoter of equine hexokinase II gene. 马己糖激酶II基因cDNA及近端启动子序列分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-06-01 DOI: 10.1080/10425170601136648

Tetsuo Sato, Takuya Itou, Go Sato, Yuki Kobayashi, Hideki Endo, Takeo Sakai

引用次数: 1

Cloning, sequence analysis and homology modeling of a novel phospholipase A2 from Heterometrus fulvipes (Indian black scorpion). 一种新的印度黑蝎磷脂酶A2的克隆、序列分析及同源性建模。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-06-01 DOI: 10.1080/10425170701243294

Gururao Hariprasad, Baskar Singh, Utpal Das, Abdul S Ethayathulla, Punit Kaur, Tej P Singh, Alagiri Srinivasan

引用次数: 22

Sequence analysis of a human RhoGAP domain-containing gene and characterization of its expression in human multiple tissues. 人RhoGAP结构域基因序列分析及其在人多组织中的表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-06-01 DOI: 10.1080/10425170600752965

Lei Lv, Jian Xu, Shuo Zhao, Chunjing Chen, Xin Zhao, Shaohua Gu, Chaoneng Ji, Yi Xie, Yumin Mao

{"title":"Sequence analysis of a human RhoGAP domain-containing gene and characterization of its expression in human multiple tissues.","authors":"Lei Lv, Jian Xu, Shuo Zhao, Chunjing Chen, Xin Zhao, Shaohua Gu, Chaoneng Ji, Yi Xie, Yumin Mao","doi":"10.1080/10425170600752965","DOIUrl":"https://doi.org/10.1080/10425170600752965","url":null,"abstract":"Rho GTPase activating proteins (GAPs) stimulate the intrinsic GTP hydrolysis activity of Rho family proteins. Here we isolated a rhoGAP domain-containing protein gene with the same reading frame with ARHGAP19 gene, which has an ORF of 1485 bp encoding a putative protein of 494 amino acid residues with a predicted molecular mass of 55.806 kDa. Protein pattern analysis shows that it contains a bipartite nuclear localization signal (NLS) besides the rhoGAP domain, and it is consistent with the result of sub-cellular localization. ARHGAP19 is located in chromosome 10q24.1 and consists of 12 exons according to the Blastn result. Weak expression was detected in adult pancreas, spleen, thymus and ovary of the 16 adult tissues examined, while it had a more abundant expression pattern in eight important human fetal tissues. The expression pattern of ARHGAP19 shows it may have functions related to fetus development and gives us some clues on its probable functions in adult tissues.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 3","pages":"184-9"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170600752965","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26682724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells. BY-2 细胞中的激发素反应性凝集素样受体激酶基因

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601060905

Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

引用次数: 24

Molecular cloning and characterization of a cDNA encoding a novel antibacterial peptide, defensin, from the mulberry longicorn beetle, Apriona germari. 桑蚕天牛新抗菌肽防御素cDNA的克隆及特性研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601108639

Wei Lu, Zheng Cui, Dong Wang, Kwang Sik Lee, Nam Sook Park, Doh Hoon Kim, Byung Rae Jin

引用次数: 2

Cloning and characterization of a putative 12-oxophytodienoic acid reductase cDNA induced by osmotic stress in roots of foxtail millet. 渗透胁迫诱导谷子根部12-氧植物二烯酸还原酶cDNA的克隆与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601060764

Jin-Peng Zhang, Ting-Song Liu, Jun Zheng, Zheng Jin, Yun Zhu, Jiu-Feng Guo, Guo-Ying Wang

{"title":"Cloning and characterization of a putative 12-oxophytodienoic acid reductase cDNA induced by osmotic stress in roots of foxtail millet.","authors":"Jin-Peng Zhang, Ting-Song Liu, Jun Zheng, Zheng Jin, Yun Zhu, Jiu-Feng Guo, Guo-Ying Wang","doi":"10.1080/10425170601060764","DOIUrl":"https://doi.org/10.1080/10425170601060764","url":null,"abstract":"Foxtail millet is a gramineous crop with low water requirement. Cloning of osmotic responses-related genes from foxtail millet is a key step for understanding the mechanism of its tolerance to drought. Here we reported the cloning and characterization of a cDNA (SiOPR1) encoding a putative 12-oxophytodienoic acid reductase 1 from foxtail millet by using RACE methods. Sequence analysis showed that SiOPR1 encoded a polypeptide of 374 amino acids with a predicted molecular mass of 41.9 kDa and pI of 5.14. Multiple alignment result showed that OPR1 protein was very conservative among gramineous crops. RNA gel blot analysis results indicated that SiOPR1 was up-regulated by osmotic stress, and its expression was limited in the roots of foxtail millet. However, SiOPR1 expression was not affected by ABA, NaCl and MeJA treatments both in roots and shoots. Therefore, it is suggested that SiOPR1 gene play an important role in response to drought stress.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 2","pages":"138-44"},"PeriodicalIF":0.0,"publicationDate":"2007-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601060764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26608507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Identification and characterization of a cDNA encoding the acetylcholinesterase of Haematobia irritans (L.) (Diptera: Muscidae). 刺激嗜血杆菌乙酰胆碱酯酶cDNA的鉴定与鉴定(双翅目:蝇科)。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601060558

Kevin B Temeyer, Andrew C Chen

引用次数: 16

Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua. 甜菜夜蛾滞育激素及信息素生物合成激活神经肽基因的克隆与表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601060806

Jun Xu, Jian-Ya Su, Jin-Liang Shen, Wei-Hua Xu

{"title":"Cloning and expression of the gene encoding the diapause hormone and pheromone biosynthesis activating neuropeptide of the beet armyworm, Spodoptera exigua.","authors":"Jun Xu, Jian-Ya Su, Jin-Liang Shen, Wei-Hua Xu","doi":"10.1080/10425170601060806","DOIUrl":"https://doi.org/10.1080/10425170601060806","url":null,"abstract":"Diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN), two important insect neuropeptides, regulate insect development and sex pheromone biosynthesis, respectively. DH-like immunoreactivity has been detected in the suboesophageal ganglion (SG) of pharate adult of Spodoptera exigua (Spe) by using an antiserum against Helicoverpa armigera DH. A full-length of Spe-DH-PBAN cDNA was obtained based on reverse transcription-PCR and rapid amplification of cDNA ends strategies. The open reading frame of this cDNA encodes a 197-amino acid precursor protein that contains DH, PBAN, and three other SG neuropeptides, all of which share a conservative C-terminal pentapeptide motif FXPR/KL (X = G, T or S). Northern blot analysis demonstrates the presence of an 800 bp transcript in the SG. The Spe-DH-PBAN mRNA is detectable at high levels at larval and adult stages, suggesting that Spe-DH-PBAN gene might be correlated with larval development and sex pheromone biosynthesis in moths.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 2","pages":"145-51"},"PeriodicalIF":0.0,"publicationDate":"2007-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601060806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26608508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Cloning, sequencing of bone morphogenetic protein from sea urchin, Hemicentrotus pulcherrimus. 海胆骨形态发生蛋白的克隆与序列分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601017186

Yumi Kurogi, Kazumasa Ohta, Tohru Nakazawa, Hiroaki Tosuji

引用次数: 0

Heterotopic expression of B-class floral homeotic genes PISTILLATA/GLOBOSA supports a modified model for crocus (Crocus sativus L.) flower formation. b类花同源基因PISTILLATA/GLOBOSA的异位表达支持了藏红花(crocus sativus L.)花形成的改进模型。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-04-01 DOI: 10.1080/10425170601060582

Apostolos Kalivas, Konstantinos Pasentsis, Alexios N Polidoros, Athanasios S Tsaftaris

{"title":"Heterotopic expression of B-class floral homeotic genes PISTILLATA/GLOBOSA supports a modified model for crocus (Crocus sativus L.) flower formation.","authors":"Apostolos Kalivas, Konstantinos Pasentsis, Alexios N Polidoros, Athanasios S Tsaftaris","doi":"10.1080/10425170601060582","DOIUrl":"https://doi.org/10.1080/10425170601060582","url":null,"abstract":"For uncovering and understanding the molecular mechanisms controlling flower development in cultivated Crocus sativus and particularly the transformation of sepals in outer whorl (whorl 1) tepals, we have cloned and characterized the expression of a family of five PISTILLATA/GLOBOSA-like (PI/GLO-like) MADS-box genes expressed in the C. sativus flower. The deduced amino acid sequences of the coded proteins indicated high homology with members of the MADS-box family of transcription factors, and particularly with other members of the PI/GLO family of MADS-box proteins that control floral organ identity. PI/GLO expression studies in cultivated C. sativus uncover the presence of PI/GLO transcripts not only in the second and third whorls of flower organs as expected, but also in the outer whorl tepals that are the sepals in most typical flowers. This heterotopic expression of both B-class genes: PI/GLO and AP3/DEF, known to form heterodimers for stamens and petals (petaloid inner whor l-whorl 2-tepals in C. sativus), explains the homeotic transformation of sepals into outer whorl tepals in this species. Analysis of PI/GLO sequences from C. sativus for putative targets to known micro-RNAs (miRNAs) showed that the target site for ath-miRNA167 found in Arabidopsis thaliana PI is not present in C. sativus, however, the PI/GLO sequences may be regulated by an ath-miRNA163.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 2","pages":"120-30"},"PeriodicalIF":0.0,"publicationDate":"2007-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601060582","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26609143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32