DNA sequence : the journal of DNA sequencing and mapping最新文献_第6页

Isolation, sequence analysis and expression profile of a novel porcine gene, CXCL10, differentially expressed in the Longissimus dorsi muscle tissues from Meishan, Meishan x Large White cross and Large White pigs. 梅山、梅山×大白猪和大白猪背最长肌组织差异表达猪新基因CXCL10的分离、序列分析及表达谱

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701243328

G Y Liu, Y Z Xiong

{"title":"Isolation, sequence analysis and expression profile of a novel porcine gene, CXCL10, differentially expressed in the Longissimus dorsi muscle tissues from Meishan, Meishan x Large White cross and Large White pigs.","authors":"G Y Liu, Y Z Xiong","doi":"10.1080/10425170701243328","DOIUrl":"https://doi.org/10.1080/10425170701243328","url":null,"abstract":"In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Meishan (MS), Meishan x Large White (ML) cross and Large White (LW) pigs. One novel gene that was differentially expressed was identified using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and its full-length cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 104 amino acids that contains the putative conserved domain of the chemokine CXC which could be designated as chemokine cd00273 subgroup and this protein has high homology with the small inducible cytokine B10 precursor (CXCL10) of five species - dog(87%), human (84%), monkey (84%), mouse (75%) and rat (70%) - so that it can be defined as swine small inducible cytokine B10 precursor. The phylogenetic tree analysis revealed that the swine CXCL10 has a closer genetic relationship with the CXCL10 of dog than with those of human, monkey, mouse and rat. The tissue expression analysis indicated that the swine CXCL10 gene is more highly expressed in muscle and weakly expressed in fat and kidney. The genomic sequence of swine CXCL10 gene was finally amplified and result revealed that the swine CXCL10 gene contains four exons and three introns. Our experiment is the first to establish the primary foundation for further research on the swine CXCL10 gene.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 6","pages":"415-22"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701243328","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Isolation and expression of GA 2-oxidase2 in tomato. GA 2-氧化酶2在番茄中的分离与表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701462183

J H Xiao, J H Zhang, Y Y Zhang, T T Wang, R G Chen, H X Li, Z B Ye

引用次数: 1

Molecular cloning and expression analysis of chicken MyD88 and TRIF genes. 鸡MyD88和TRIF基因的克隆及表达分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701295856

Sarah Wheaton, Melissa D Lambourne, Aimie J Sarson, Jennifer T Brisbin, Ashraf Mayameei, Shayan Sharif

{"title":"Molecular cloning and expression analysis of chicken MyD88 and TRIF genes.","authors":"Sarah Wheaton, Melissa D Lambourne, Aimie J Sarson, Jennifer T Brisbin, Ashraf Mayameei, Shayan Sharif","doi":"10.1080/10425170701295856","DOIUrl":"https://doi.org/10.1080/10425170701295856","url":null,"abstract":"Toll-like receptors (TLRs) trigger the innate immune system by responding to specific components of microorganisms. MyD88 and TRIF are Toll/interleukin (IL)-1 (TIR)-domain containing adapters, which play essential roles in TLR-mediated signalling via the MyD88-dependant and -independent pathways, respectively. Genes encoding several TLRs have been identified in the chicken genome, however, elements of their signalling pathways have not been well characterized. Here we describe the cloning of chicken MyD88 and TRIF orthologs, and examine the spatial and temporal expression of these genes. The chicken MyD88 cDNA was shown to have an open reading frame (ORF) of 1104 bp, encoding a predicted protein sequence of 368 aa, 8 aa short of a previously published coding sequence due to a premature stop codon. MyD88 gene expression was detected in each tissue tested except in muscle. The chicken TRIF cDNA possessed an ORF of 2205 bp, encoding a predicted protein sequence of 735 aa, which shared 37.3% similarity and 28.9% identity to human TRIF protein sequence. TRIF was ubiquitously expressed in all tissues.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 6","pages":"480-6"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701295856","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). 玉米花期干旱胁迫诱导基因的分离与特性分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701292051

Hui-Yong Li, Tian-Yu Wang, Yun-Su Shi, Jun-Jie Fu, Yan-Chun Song, Guo-Ying Wang, Yu Li

{"title":"Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays).","authors":"Hui-Yong Li, Tian-Yu Wang, Yun-Su Shi, Jun-Jie Fu, Yan-Chun Song, Guo-Ying Wang, Yu Li","doi":"10.1080/10425170701292051","DOIUrl":"https://doi.org/10.1080/10425170701292051","url":null,"abstract":"Maize female organs are sensitive to drought stress, leading to reproductive failure and yield reduction. In the present study gene expression profiles of ears and silks of maize at the flowering stage under drought stress were investigated. From 1920 white positive clones of a forward suppression subtractive hybridization (SSH) library, 1439 available sequences of expression sequence tags (ESTs) were obtained, resulting in 361 unique ESTs after assembling. Data analysis showed that 218 of the unique ESTs had significant protein homology by BLASTX in UNIPROT database. Totally 99 uniESTs were found in TIGR maize gene indices and nr database by BLASTN, while 44 uniESTs were not found to have homologous nucleic acid sequences and putatively classified as \"maize-specific\" uniESTs. The 218 cDNAs with significant protein homology were sorted into 13 groups according to the functional categories of the Arabidopsis proteins. Among those genes, the genes associated with the metabolisms were the largest group (account for 27%), and the genes related to protein synthesis, protein fate, transcription, cell cycle and DNA processing accounted for 16, 10, 10 and 9%, respectively. After analysis of macroarray data and real-time quantitative polymerase chain reaction (PCR), it was found that 160 of the 218 homologous protein uniESTs were up-regulated genes in the ears, 129 in the silks, and 125 in both of the tissues. The present work provided a valuable starting point for further elucidation of the roles played by these genes/gene products in drought tolerance in maize.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 6","pages":"445-60"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701292051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Complete mitochondrial genome of a Chinese scorpion Mesobuthus martensii (Chelicerata, Scorpiones, Buthidae). 马氏中蝎(螯角目，蝎科，蝎科)线粒体全基因组的研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701289883

Eun Hwa Choi, Shin Ju Park, Keum Hee Jang, Wook Hwang

引用次数: 20

Isolation and characterization of SlIAA3, an Aux/IAA gene from tomato. 番茄Aux/IAA基因SlIAA3的分离与特性分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701517820

Junhong Zhang, Rugang Chen, Jinhua Xiao, Liping Zou, Hanxia Li, Bo Ouyang, Zhibiao Ye

引用次数: 10

Molecular cloning and characterization of a putative protein kinase gene from the thermophilic fungus Thermomyces lanuginosus. 嗜热真菌热酵母菌蛋白激酶基因的克隆与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701318443

Duo-Chuan Li, Wei Li, Qing-Xin Zhou, Jing Lu, You-Liang Peng

{"title":"Molecular cloning and characterization of a putative protein kinase gene from the thermophilic fungus Thermomyces lanuginosus.","authors":"Duo-Chuan Li, Wei Li, Qing-Xin Zhou, Jing Lu, You-Liang Peng","doi":"10.1080/10425170701318443","DOIUrl":"https://doi.org/10.1080/10425170701318443","url":null,"abstract":"Based on the conserved amino acid sequence (DLKPEN) of serine-threonine protein kinase from several fungi, a degenerate primer was designed and synthesized. Total RNA was isolated from the thermophilic fungus Thermomyces lanuginosus. Using RACE-PCR, full-length cDNA of a putative serine-threonine protein kinase gene was cloned from T. lanuginosus. The full-length cDNA of T. lanuginosus protein kinase was 2551 bp and contained an 1806 bp open reading frame encoding a putative protein kinase precursor of 601 amino acid residues. Sequencing analysis showed that the cloned cDNA of T. lanuginosus had consensus protein kinase sequences. Conservative amino acid subdomains which most serine-threonine kinases contain can be found in the deduced amino acid sequence of T. lanuginosus putative protein kinase. Comparison results showed that the deduced amino acid sequence of T. lanuginosus putative protein kinase was highly homologous to that of Neurospora crassa dis1-suppressing protein kinase Dsk1. The putative protein kinase contained three arginine/serine-rich (SR) regions and two transmembrane domains. These showed that it might be a novel putative serine-threonine protein kinase.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 6","pages":"423-33"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701318443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Corticotropin releasing hormone (CRH) gene variation: comprehensive resequencing for variant and molecular haplotype discovery in monosomic hybrid cell lines. 促肾上腺皮质激素释放激素(CRH)基因变异:单体杂交细胞系变异和分子单倍型发现的综合重测序。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-12-01 DOI: 10.1080/10425170701388719

Lawrence C Shimmin, Sivamani Natarajan, Heladio Ibarguen, May Montasser, Do-Kyun Kim, Craig L Hanis, Eric Boerwinkle, Pathik D Wadhwa, James E Hixson

{"title":"Corticotropin releasing hormone (CRH) gene variation: comprehensive resequencing for variant and molecular haplotype discovery in monosomic hybrid cell lines.","authors":"Lawrence C Shimmin, Sivamani Natarajan, Heladio Ibarguen, May Montasser, Do-Kyun Kim, Craig L Hanis, Eric Boerwinkle, Pathik D Wadhwa, James E Hixson","doi":"10.1080/10425170701388719","DOIUrl":"https://doi.org/10.1080/10425170701388719","url":null,"abstract":"Candidate gene association studies have met with mixed success due to many reasons including incomplete surveys of genetic variation and differences in patterns of genetic variation among study populations. We present the results of comprehensive variant discovery for the corticotropin releasing hormone gene (CRH on chromosome 8) encoding a neuropeptide that is central to many physiologic pathways. Mouse-human hybrid cell lines were constructed that are monosomic for human chromosome 8 for resequencing of separated CRH alleles to identify variants and directly determine their chromosomal phase for three major ethnic groups including African Americans (AA), Mexican Americans (MA) and European Americans (EA). We also resequenced diploid individuals to evaluate single nucleotide polymorphism (SNP) discovery in the limited numbers of monosomic hybrid cell lines. Our results show that CRH variation is very different in AA, yielding larger numbers of variants and haplotypes compared to MA and EA. Analysis of LD structure found three haplotype blocks in AA and two blocks in EA. Comparisons between AA and EA groups yielded extremely high measures of genetic differentiation (Wright's F(ST)>0.6), likely reflecting disruptive selection in CRH evolution. Network analysis showed that AA have retained an ancestral CRH haplotype, while the most common EA haplotype is derived from a single recombination event.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 6","pages":"434-44"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701388719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Verification and tissue-specific expression of nifU-like gene from the amphioxus Branchiostoma belcheri. 文昌鱼belcheri Branchiostoma nifU-like基因的验证及组织特异性表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701400308

Lei Li, Shicui Zhang, Chunxin Fan, Zhenhui Liu, Jing Luan

引用次数: 0

Cloning, characterization and differential expression of an hsp70 gene from the pathogenic dimorphic fungus, Penicillium marneffei. 致病性二态真菌马尔尼菲青霉hsp70基因的克隆、鉴定及差异表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2007-10-01 DOI: 10.1080/10425170701309012

Aksarakorn Kummasook, Patthama Pongpom, Nongnuch Vanittanakom

{"title":"Cloning, characterization and differential expression of an hsp70 gene from the pathogenic dimorphic fungus, Penicillium marneffei.","authors":"Aksarakorn Kummasook, Patthama Pongpom, Nongnuch Vanittanakom","doi":"10.1080/10425170701309012","DOIUrl":"https://doi.org/10.1080/10425170701309012","url":null,"abstract":"A gene encoding heat shock protein 70 (Hsp70) of Penicillium marneffei was isolated and characterized. The structure of P. marneffei hsp70 gene was similar to hsp70 genes of other organisms, with a unique sequence of 3-nt microexon flanked by two introns. Comparison of the deduced amino acid sequence revealed that the Hsp70 was grouped in the fungal cytosolic Hsp70s. Northern blot analysis demonstrated the upregulation of hsp70 expression during the mycelium to yeast phase transition. Upregulation was also observed during yeast or mycelial cells encountering heat shock condition at 39 degrees C. Experimental analysis showed that the expression of hsp70 is temperature dependent. Contradictory, a severe heat shock condition at 42 degrees C resulted in lowering the hsp70 transcript. Reverse transcription-polymerase chain reaction (RT-PCR) showed the accumulation of a large population of mature mRNA and small population of intron II-unspliced hsp70 mRNA in most cell types (conidia, mycelia and yeast). These results suggested that the Hsp70 may play an important role in environmental stress response and adaptation.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"18 5","pages":"385-94"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701309012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26850072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26