DNA sequence : the journal of DNA sequencing and mapping最新文献_第5页

Sequencing, characterization and genetic variation of the Bos indicus glucose-6-phosphate-dehydrogenase gene. 马铃薯葡萄糖-6-磷酸脱氢酶基因的测序、鉴定和遗传变异。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701283977

G V P P S Ravi Kumar, G Srinivas, A Sharma, V V S Suryanarayana, P Ravi Kumar, T K Bhattacharya, A Mitra

{"title":"Sequencing, characterization and genetic variation of the Bos indicus glucose-6-phosphate-dehydrogenase gene.","authors":"G V P P S Ravi Kumar, G Srinivas, A Sharma, V V S Suryanarayana, P Ravi Kumar, T K Bhattacharya, A Mitra","doi":"10.1080/10425170701283977","DOIUrl":"https://doi.org/10.1080/10425170701283977","url":null,"abstract":"The coding sequence of the bovine (Bos indicus) Glucose-6-phosphate-dehydrogenase (G6PD) gene was amplified by Reverse Transcriptase-PCR (RT-PCR), cloned, sequenced and characterized. The deduced amino acid sequence clustered the bovine G6PD sequence with the other mammalian G6PD proteins into a monophyletic group. The bovids (B. indicus and B. taurus) clustered clearly from the rodent (rat, mouse and hamster) subcluster and from humans. The multiple sequence alignment of the bovine G6PD with the mammalian species clearly revealed conservation of the substrate, coenzyme, catalytic and the dimer binding sites with the solved X-ray crystallographic structure of Homo sapiens. Also, four fragments of bovine (Bos indicus) G6PD gene viz. 118, 319, 683 and 408 bp were amplified and sequenced for the first time. A G/A and G/C single nucleotide polymorphisms in intron-9 and exon-10 were detected on PCR-RFLP of the 319 bp amplicon with Hae III and Pst I, respectively. This work is the first study on Bos indicus G6PD gene at the nucleotide level.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"19 1","pages":"37-43"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701283977","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27286397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Verification and tissue-specific expression of nifU-like gene from the amphioxus Branchiostoma belcheri. 文昌鱼belcheri Branchiostoma nifU-like基因的验证及组织特异性表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170601176875

Lei Li, Shicui Zhang, Chunxin Fan, Zhenhui Liu, Jing Luan

引用次数: 0

Identification of coding polymorphisms in human circadian rhythm genes PER1, PER2, PER3, CLOCK, ARNTL, CRY1, CRY2 and TIMELESS in a multi-ethnic screening panel. 在多种族筛选小组中鉴定人类昼夜节律基因 PER1、PER2、PER3、CLOCK、ARNTL、CRY1、CRY2 和 TIMELESS 的编码多态性。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701322197

Gregory A Hawkins, Deborah A Meyers, Eugene R Bleecker, Allan I Pack

{"title":"Identification of coding polymorphisms in human circadian rhythm genes PER1, PER2, PER3, CLOCK, ARNTL, CRY1, CRY2 and TIMELESS in a multi-ethnic screening panel.","authors":"Gregory A Hawkins, Deborah A Meyers, Eugene R Bleecker, Allan I Pack","doi":"10.1080/10425170701322197","DOIUrl":"10.1080/10425170701322197","url":null,"abstract":"Study objective: In this study, the exonic regions of the circadian rhythm genes PER1, PER2, PER3, CLOCK, ARNTL, CRY1, CRY2 and TIMELESS were re-sequenced and coding changes identified in a panel of 95 individuals varying in ethnicity.Study participants: DNA screening panel consisting of 95 DNA samples (17 American Caucasians, 17 African Americans, 8 Ashkenazi Jews, 8 Chinese, 8 Japanese, 5 Mexican Indians, 8 Mexicans, 8 Northern Europeans, 8 Puerto Ricans, and 8 South Americans) selected from the Coriell Institute Human Variation Panel.Results: In addition to coding changes already identified in the database dbSNP, novel coding changes were identified, including PER1: Pro37Ser, Pro351Ser, Gln988Pro, Ala998Thr; PER2: Leu83Arg, Leu157Leu, Thre174Ile, Phe400Phe, Pro822Pro, Ala828Thr, Ala861Val, Phe876Leu, Val883Met, Val903Ile, Ala923Pro; PER3: Pro67Pro, Val90Ile, His638His, Ala820Ala, Leu929Leu; ARNTL: Arg166Gln, Ser459Phe; CLOCK: Ala34Ala, Ser208Cys, Phe233Phe, Ser632Thr, Ser816Ser; TIMELESS: Met870Val and CRY2: His35His. No coding polymorphisms were identified in CRY1.Conclusions: Considerable genetic variation occurs within the coding region of the genes regulating circadian rhythm. Many of the non-synonymous coding polymorphisms could affect protein structure/function with the potential to affect molecular regulation of the sleep/wake cycle. Many of the potential functional effects could be ethnic group specific.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":" ","pages":"44-9"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701322197","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40959609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Cloning and expression of translation elongation factor 2 (EF-2) in zebrafish. 翻译延伸因子2 (EF-2)在斑马鱼中的克隆与表达。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170500332314

Shu-Hong Zhang, Ji-Hua Yao, Huai-Dong Song, Lu Wang, Jing-Lun Xue

{"title":"Cloning and expression of translation elongation factor 2 (EF-2) in zebrafish.","authors":"Shu-Hong Zhang, Ji-Hua Yao, Huai-Dong Song, Lu Wang, Jing-Lun Xue","doi":"10.1080/10425170500332314","DOIUrl":"https://doi.org/10.1080/10425170500332314","url":null,"abstract":"We have identified a developmentally regulated gene translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession No. AAQ91234). Analysis of DNA sequence of zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame from nucleotide 55 to 2631 and encodes a protein of 858 amino acids. It shares an identity of 92, 93, 93, 92, 79 and 80% in amino acid sequence to human, mouse, Chinese hamster, Gallus gullus, C. elegans and Drosophila EF-2, respectively. Zebrafish EF-2 protein has 16 conserved domains, GTP-binding domain is found in the NH2 terminus, and the ADP-ribosylation domain locates at the COOH terminus. Whole mount in situ hybridization on zebrafish embryos shows that the transcripts of EF-2 gene are detected during the early development of zebrafish embryo and constantly change from 5-somite stage to protruding-mouth stage. It expresses strongly throughout envelope at 5-somite stage. Then the stained cells concentrate strongly in the eyes, brain and muscle tissue. From prim-25 stage the stained cells only appear strongly in the lens and the anterior portion of the cerebellum.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"19 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170500332314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27285881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of a seed-specific isoform of microsomal omega-6 fatty acid desaturase gene (FAD2-1B) from soybean. 大豆微粒体欧米伽-6 脂肪酸去饱和酶基因（FAD2-1B）种子特异性同工型的分离与鉴定。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701207208

Lingyong Li, Xiaolin Wang, Junyi Gai, Deyue Yu

{"title":"Isolation and characterization of a seed-specific isoform of microsomal omega-6 fatty acid desaturase gene (FAD2-1B) from soybean.","authors":"Lingyong Li, Xiaolin Wang, Junyi Gai, Deyue Yu","doi":"10.1080/10425170701207208","DOIUrl":"10.1080/10425170701207208","url":null,"abstract":"In plants, the endoplasmic reticulum (ER)-associated oleate desaturase (FAD2) is the key enzyme responsible for the production of linoleic acid in non-photosynthetic tissues. In this study, we report the characterization of a seed-specific isoform of microsomal omega-6 fatty acid desaturase gene (FAD2-1B) sharing high sequence similarity with FAD2-1 from soybean. Several potential promoter elements including seed-specific motifs are found in the 5'-flanking region of FAD2-1B gene. The ORF of FAD2-1B is 1161 bp long and encodes a protein of 387 amino acids. This deduced protein holds three histidine boxes and four putative membrane-spanning helices, and possesses a signal for endoplasmic reticulum retention at C-terminal. Yeast cells transformed with the plasmid construct containing soybean FAD2-1B accumulate an appreciable amount of linoleic acid (18:2), normally not present in wild-type yeast cells, indicating that the cloned gene encodes a functional FAD2 enzyme. Both semi-quantitative RT-PCR and in silico analysis show that FAD2-1B gene is specifically expressed in developing seeds of soybean.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":"19 1","pages":"28-36"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170701207208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27286396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cloning and characterization of a novel human SPRYD4 gene encoding a putative SPRY domain-containing protein. 一个新的人类SPRYD4基因的克隆和特性，该基因编码一个假定的SPRY结构域蛋白。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701433101

Zhaomin Zhong, Haoxing Zhang, Meirong Bai, Jun Ni, Bo Wan, Xinya Chen, Long Yu

引用次数: 3

Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus). 南极掠食鱼(Harpagifer antarcticus)温热适应蛋白基因(wap65)的研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701388586

Melody S Clark, Gavin Burns

引用次数: 15

Sequence analysis and characterization of vacuolar-type H+ -ATPase proteolipid transcript from Acanthus ebracteatus Vah1. 棘猴Vah1液泡型H+ - atp酶蛋白脂转录物的序列分析与特性研究。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701445501

Chai-Ling Ho, Phuoc Dang Nguyen, Jennifer Ann Harikrishna, Raha Abdul Rahim

引用次数: 2

1A and 3D gene sequences of coxsackievirus B3 strain CC: variation and phylogenetic analysis. 柯萨奇病毒B3株CC的1A和3D基因序列:变异和系统发育分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170601101428

Ming-Yu Liu, Dong-Lai Wu, Ni-Hong Liu, Qing-Wen Meng, Fan-Chao Meng

{"title":"1A and 3D gene sequences of coxsackievirus B3 strain CC: variation and phylogenetic analysis.","authors":"Ming-Yu Liu, Dong-Lai Wu, Ni-Hong Liu, Qing-Wen Meng, Fan-Chao Meng","doi":"10.1080/10425170601101428","DOIUrl":"https://doi.org/10.1080/10425170601101428","url":null,"abstract":"Coxsackievirus B3 (CVB3) was thought to be the most common causative agent of life-threatening viral myocarditis. Coxsackievirus B3 strain CC (CVB3-CC) was isolated in China; however, no sequence data are available. The 1A and 3D regions of CVB3-CC were sequenced and phylogenetic analysis was done with reference to ten other CVB3 strains and all 36 prototype strains of human enterovirus B (HEV-B). Sequence analysis showed that the 1A gene region of CVB3-CC consisted of 207 nucleotides, encoding 69 amino acids; and the 3D gene region was comprised of 1386 nucleotides, encoding 462 amino acids. Variation analysis showed that the 3D gene of CVB3 strain CC varied the least among the two regions. Phylogenetic tree analysis of the 1A and 3D regions indicated that CVB3-CC clustered together with CVB3 Nancy strain suggesting that there may be a close evolutionary relationship between the two strains. Incongruity was observed between the non-structural protein gene and the structural protein gene trees, according to the topological structure, indicating that recombination was occurred among these strains.","PeriodicalId":11197,"journal":{"name":"DNA sequence : the journal of DNA sequencing and mapping","volume":" ","pages":"8-12"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10425170601101428","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40960081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cloning and functional analysis of the swine eNOS promoter. 猪eNOS启动子的克隆及功能分析。

DNA sequence : the journal of DNA sequencing and mapping Pub Date : 2008-02-01 DOI: 10.1080/10425170701400183

Kimberly M Shontz, Bi Zhou, C Yung Yu, Baogen Y Su

引用次数: 1