Dental and Medical Problems最新文献

{"title":"Laser therapies for oral lichen planus: An overview of systematic reviews and meta-analyses with evidence-based recommendations from the Polish Society for Laser Dentistry.","authors":"Kinga Grzech-Leśniak, Rafał Wiench, Jakub Fiegler-Rudol, Zuzanna Grzech-Leśniak, Wojciech Niemczyk, Grzegorz Marek, Bartłomiej Pokrzywka, Adam Ziemlewski, Dariusz Roman Skaba, Jacek Matys, Alina Jankowska-Konsur, Halina Bubala, Marek Ussowicz, Tomasz Wróbel, Krzysztof Dowgierd, Mariusz Szuta, Marzena Dominiak","doi":"10.17219/dmp/216723","DOIUrl":"https://doi.org/10.17219/dmp/216723","url":null,"abstract":"<p><p>The objective of the study was to comprehensively synthesize and critically evaluate the effectiveness, safety and clinical applicability of various laser therapies in the management of oral lichen planus (OLP), including photodynamic therapy (PDT), photobiomodulation therapy (PBMT) and high-intensity laser therapy (HILT), and to provide guidelines for clinicians.A structured literature review was conducted following the PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) guidelines, and recommendations were formulated. A total of 23 relevant studies published between 2015 and 2025 were identified through searches of multiple biomedical databases. The risk of bias and quality of evidence were assessed, focusing on clinical outcomes such as reduction in lesion severity, pain relief, mucosal healing, and recurrence rates.Laser therapies demonstrated effectiveness in reducing lesion size and pain, with safety profiles comparable to or superior to those of conventional corticosteroid treatments. Photobiomodulation therapy and HILT showed particularly favorable outcomes, including lower recurrence rates and fewer adverse effects. However, heterogeneity in treatment protocols and laser parameters limited direct comparisons, with corticosteroids remaining the first-line treatment.Laser-based therapies are valuable adjuncts or alternatives for patients with OLP who are intolerant to corticosteroids. Standardized treatment protocols and further large-scale randomized controlled trials with longer follow-up periods are needed to optimize clinical guidelines and enable personalized therapy. Photobiomodulation therapy is recommended as the safest and most effective modality for erosive OLP.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of photobiomodulation therapy and occlusal splints in the treatment of bruxism in children: A systematic review.","authors":"Souad Al Fallaha, Ramah Eimad Makieh, Chaza Nader Kouchaji, Dina Aly Sharaf, Omar Hamadah","doi":"10.17219/dmp/185318","DOIUrl":"https://doi.org/10.17219/dmp/185318","url":null,"abstract":"<p><p>Untreated sleep bruxism (SB) can cause damage to the stomatognathic system, potentially reducing academic performance in children and negatively affecting their quality of life. Occlusal splints are considered the gold standard for the treatment of bruxism in children; however, they present many limitations, prompting the search for alternative therapies. Laser therapy has been proposed as a more favorable, painless and cost-effective treatment option for pediatric patients. Therefore, the aim of this study was to evaluate the effectiveness of photobiomodulation therapy (PBMT) and occlusal splints in the treatment of bruxism in children.This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. Studies were identified through searches of the following databases: MEDLINE (PubMed®); Google Scholar; and the Cochrane Library. The review included articles published between January 2010 and June 2023. Studies evaluating the treatment of bruxism using occlusal splints and/or PBMT in participants under 18 years of age were included.A total of 67 articles published in English were identified. After applying the eligibility criteria and assessing study quality using the Cochrane risk-of-bias tool, 6 studies were included in the final analysis. Of these, 4 investigated various types of occlusal splints, while 2 compared PBMT with occlusal splints. The papers also examined diagnostic methods, treatment outcomes, salivary cortisol levels, and dopamine levels.Occlusal splints have limited effectiveness in managing the signs and symptoms of bruxism. Photobiomodulation therapy shows potential as a treatment method, but its effectiveness remains inconclusive. Due to the limited number of available studies, definitive conclusions cannot be drawn, and further research is necessary.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and cytotoxic evaluation of biosurfactant-coated copper nanoparticles: A study on selected endodontic pathogens.","authors":"Hmoud Ali Algarni, Meshal Aber Alonazi, Shaeesta Khaleelahmed Bhavikatti, Abdul Habeeb Adil, Mohmed Isaqali Karobari","doi":"10.17219/dmp/199972","DOIUrl":"https://doi.org/10.17219/dmp/199972","url":null,"abstract":"<p><strong>Background: </strong>Endodontic infections caused by pathogens such as Escherichia coli, Staphylococcus aureus, Streptococcus oralis, and Enterococcus spp. pose significant challenges due to biofilm formation and resistance to conventional treatments. Copper nanoparticles (CuNPs) exhibit promising antimicrobial properties, and coating them with biosurfactants may enhance stability while reducing cytotoxicity.</p><p><strong>Objectives: </strong>The aim of the study was to synthesize and characterize CuNPs stabilized with biosurfactants derived from Achromobacter xylosoxidans and Pseudomonas aeruginosa, and to evaluate their antimicrobial efficacy and cytotoxicity.</p><p><strong>Material and methods: </strong>Copper nanoparticles were synthesized using a chemical reduction method and coated with biosurfactants to prevent oxidation and aggregation. The nanoparticles were characterized using Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Antimicrobial activity against E. coli, S. aureus, S. oralis, and Enterococcus spp. was evaluated using the well diffusion method and the minimum inhibitory concentration (MIC). Cytotoxicity was assessed using an MTT assay on L929 fibroblast cell lines.</p><p><strong>Results: </strong>Characterization confirmed the formation of spherical CuNPs with sizes ranging from 90 nm to 100 nm. Both CuO+P5C and CuO+P11 nanoparticles demonstrated potent antimicrobial activity against all tested pathogens, with a MIC value of 125 μg/mL. Cytotoxicity results showed minimal toxicity, with nearly 78-80% cell viability maintained at concentrations of up to 100 μg/mL, indicating excellent biocompatibility.</p><p><strong>Conclusions: </strong>Biosurfactant-coated CuNPs represent a stable, eco-friendly and effective antimicrobial alternative for addressing persistent endodontic infections. Further in vivo studies and extended cytotoxicity evaluations are needed to confirm their clinical application.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of multiple gingival recessions, using the modified coronally advanced tunnel technique and subepithelial connective tissue graft with or without 24% EDTA root conditioning: 3- and 5-year outcomes of a randomized clinical trial.","authors":"Nela Molga-Chlipała, Bartłomiej Górski, Kacper Nijakowski, Darco Bozic","doi":"10.17219/dmp/216999","DOIUrl":"https://doi.org/10.17219/dmp/216999","url":null,"abstract":"<p><strong>Background: </strong>Gingival recession (GR) represents a highly common mucogingival condition in the permanent dentition in the general population.</p><p><strong>Objectives: </strong>The aim of this randomized, split-mouth clinical trial was to assess the 3- and 5-year results of the treatment of multiple GRs through modified coronally advanced tunnel technique (MCAT) and subepithelial connective tissue graft (SCTG) with or without 24% ethylenediaminetetraacetic acid (EDTA) root conditioning.</p><p><strong>Material and methods: </strong>Twenty patients with 142 GRs received both test and control treatment (72 test sites: MCAT+SCTG+EDTA, and 70 control sites: MCAT+SCTG). Two patients were lost to follow-up before the 3-year mark; therefore, a total of 125 GRs were analyzed in 18 subjects. The mean and complete root coverage (MRC, CRC), gain in the clinical attachment level (CAL), gain in keratinized tissue width (KTW), gain in gingival thickness (GT), the root esthetic coverage score (RES), and patient-reported outcome measures (PROMs) were evaluated.</p><p><strong>Results: </strong>All parameters showed significant improvement in both groups as compared with baseline. There were no statistically significant differences between 3 and 5 years within the groups except for GT, or between the groups at either time point. In the test group, MRC was 95.8% at 3 years and 94.2% at 5 years, with CRC obtained in 87.3% and 84.1% of GRs, respectively. In the control group, MRC was 92.6% at 3 years and 90.6% at 5 years, and CRC was achieved in 82.3% and 80.7% of GRs, respectively. Both groups achieved high RES: 9.0 and 8.9 for the MCAT+SCTG+EDTA and MCAT+SCTG sites after 3 years, and 9.1 and 9.0 after 5 years, respectively. After 5 years, the mean gingival recession height (GRH) decreased from 2.3 ±1.0 mm to 0.2 ±0.5 mm in the test group, and from 2.5 ±1.1 mm to 0.2 ±0.5 mm in the control group.</p><p><strong>Conclusions: </strong>Root surface conditioning with 24% EDTA did not contribute to the outcomes of MCAT+SCTG - 3 and 5 years postoperatively. Thus, its application in clinical practice is not justified.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification and overexpression of a novel m6A reader FXR1 are associated with poor prognosis in head and neck squamous cell carcinoma.","authors":"Jeevitha Manicka Vasagam, Balachander Kannan, Vijayashree Priyadharsini Jayaseelan, Paramasivam Arumugam","doi":"10.17219/dmp/185583","DOIUrl":"https://doi.org/10.17219/dmp/185583","url":null,"abstract":"<p><strong>Background: </strong>Fragile X-related protein 1 (FXR1), an RNA-binding protein (RBP) implicated in various cancers, has emerged as a potential contributor to the progression of head and neck squamous cell carcinoma (HNSCC).</p><p><strong>Objectives: </strong>The aim of the study was to comprehensively analyze FXR1 genetic alterations, mRNA expression, protein levels, and immune infiltration in HNSCC, in order to elucidate its molecular role and therapeutic potential.</p><p><strong>Material and methods: </strong>Data from The Cancer Genome Atlas (TCGA)-HNSCC cohort was analyzed. Copy number variation (CNV) was assessed using cBioPortal, FXR1 expression profiling was performed using the University of ALabama at Birmingham CANcer data analysis Portal (UALCAN), and immunohistochemistry (IHC) examination was conducted using the Human Protein Atlas (HPA). The TIMER 2.0 and TISIDB databases were employed for immune infiltration analysis.</p><p><strong>Results: </strong>Genetic alterations in FXR1, including DNA amplification, were observed in 22% of HNSCC patients and were significantly correlated with elevated mRNA expression. FXR1 mRNA and protein levels were upregulated in HNSCC tumors, and this upregulation was associated with poor prognosis. The expression of FXR1 was also correlated with clinical features, including disease stage, grade, metastasis, and human papillomavirus (HPV) status. Immune infiltration analysis revealed a negative correlation between FXR1 mRNA expression and CD8+ T cells, suggesting a potential role in immune modulation.</p><p><strong>Conclusions: </strong>FXR1 may serve as a potential biomarker and immune regulator in HNSCC, with genetic alterations influencing its mRNA expression. This study sheds light on the multifaceted role of FXR1 in the progression of HNSCC, and suggests potential avenues for targeted therapeutic strategies. Further research, including functional experiments and large-scale cohort analyses, is warranted to validate and translate these findings into clinical applications.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating pheophorbide-a as a photosensitizer in oral cancer photodynamic therapy: A systematic review.","authors":"Rafał Wiench, Jakub Fiegler-Rudol, Barbara Lipka, Sylwia Wójcik, Marta Bort, Martin Starosta, Won-Pyo Lee, Krzysztof Dowgierd, Tadeusz Morawiec","doi":"10.17219/dmp/216779","DOIUrl":"https://doi.org/10.17219/dmp/216779","url":null,"abstract":"<p><p>Oral squamous cell carcinoma (OSCC) is commonly treated with surgery, radiotherapy and chemotherapy, which often cause significant morbidity. Photodynamic therapy (PDT) has emerged as a minimally invasive alternative. Pheophorbide-a (Pa), a second-generation photosensitizer, shows promise, but has not been critically reviewed in oral malignancies. The aim of the present systematic review was to evaluate preclinical evidence on the mechanisms of action, efficacy and safety of Pa-mediated PDT (Pa-PDT) in oral oncology.A systematic search of PubMed, Embase, Scopus, and Cochrane Library was conducted in line with the PRISMA 2020 guidelines (PROSPERO ID: CRD420251066127). Eligible studies included in vitro or in vivo models using Pa-PDT in oral malignancies. Data on therapeutic outcomes, the mechanisms of action, and study quality was extracted and analyzed. Nine studies were included. Pheophorbide-a-induced photodynamic therapy consistently induced apoptosis, autophagy and endoplasmic reticulum (ER) stress through reactive oxygen species (ROS) and mitochondrial dysfunction. It also overcame multidrug resistance by downregulating HOXC6 and MDR1. Conjugation with targeting peptides, nanocarriers and immune checkpoint inhibitors enhanced selectivity and cytotoxicity.Pheophorbide-a demonstrated favorable photophysical properties, including strong red-light absorption (664-675nm), high singlet oxygen yield and minimal dark toxicity. Evidence was limited by small sample sizes, heterogeneous protocols and the absence of human trials. Pheophorbide-a-induced photodynamic therapy shows antitumor activity in preclinical OSCC models, supporting further preclinical development and carefully designed early-phase trials. Translation should proceed only within early-phase trials that directly benchmark Pa against the approved photosensitizers, using standardized protocols.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147688774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Romanian version of the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) Axis I: Cross-cultural adaptation, criterion validity, and reliability.","authors":"Alexandru Măzăreanu, Mieszko Więckiewicz, Dragos George Balaiasa, Maria Iacob, Alin Pandea, Tibor Szatmari, Petre Razvan Liviu, Tzvika Greenbaum","doi":"10.17219/dmp/213202","DOIUrl":"https://doi.org/10.17219/dmp/213202","url":null,"abstract":"<p><strong>Background: </strong>Temporomandibular disorders (TMD) are the most common cause of non-dental orofacial pain, affecting about 1/3 of the population worldwide. The Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) Axis I is recognized as the international gold standard for the clinical and research diagnosis of TMD; however, no validated Romanian version has been available to date.</p><p><strong>Objectives: </strong>The aim of the present study was to translate and culturally adapt the DC/TMD Axis I into Romanian, and to evaluate its criterion validity and interand intra-examiner reliability.</p><p><strong>Material and methods: </strong>This cross-sectional methodological study followed the Beaton et al. and DC/TMD translation guidelines. The process included forward-backward translation, an expert panel review, and pre-testing on 20 patients to ensure semantic and conceptual equivalence. A total of 140 Romanian-speaking adults (mean age: 34.7 ±12.1 years; 70.7% women) were examined by 2 calibrated clinicians. Criterion validity was assessed against an expert-consensus reference diagnosis. Interand intra-examiner reliability for categorical variables was analyzed using Cohen's κ, and for continuous measurements using intraclass correlation coefficients (ICCs). The Bland-Altman plots and demographic analyses further evaluated measurement consistency.</p><p><strong>Results: </strong>The Romanian DC/TMD Axis I showed excellent diagnostic performance, with sensitivity ranging from 85.7% (for disc displacement without reduction) to 91.2% (for myalgia), specificity of 93.5-98.2%, and AUC (the area under the receiver operating characteristic (ROC) curve) ≥ 0.91 for all diagnostic categories. Inter-examiner agreement was substantial to almost perfect (κ = 0.78-0.94), and intra-examiner agreement similarly high (κ = 0.80-0.95). Continuous mandibular measurements demonstrated excellent reproducibility (ICC = 0.89-0.96). Slightly lower sensitivity for disc displacement without reduction indicates an area for further refinement of diagnostic criteria.</p><p><strong>Conclusions: </strong>The Romanian version of the DC/TMD Axis I is a valid, reliable and culturally equivalent tool for diagnosing TMD. Its adoption will standardize clinical assessment, enhance interdisciplinary communication, and enable Romanian participation in multicenter and educational initiatives across Central and Eastern Europe.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo evaluation of alveolar ridge bone formation resulting from the administration of a natural propolis extract combined with a bovine xenograft.","authors":"Utari Kresnoadi, Jennifer Widjaja, Wibi Riawan","doi":"10.17219/dmp/171268","DOIUrl":"10.17219/dmp/171268","url":null,"abstract":"<p><strong>Background: </strong>Natural propolis is widely known to be an antibacterial agent with strong antioxidant and anti-inflammatory properties. In combination with a bovine xenograft, it is expected to promote bone formation in the alveolar ridge, following tooth extraction.</p><p><strong>Objectives: </strong>The aim of the present study was to evaluate the effect of administering a propolis extract combined with a bovine xenograft on alveolar bone formation.</p><p><strong>Material and methods: </strong>The lower incisor teeth of Cavia cobaya were extracted and the resulting alveolar sockets were filled with different materials based on the group assignment: polyethylene glycol (PEG); a propolis extract (PE); a bovine xenograft (BX); and the propolis extract-bovine xenograft composite (PE-BX). The alveolar sockets were examined after 7 and 30 days by means of immunohistochemical staining for the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-ĸB), tolllike receptor 2 (TLR2), tumor necrosis factor-alpha (TNF-α), and type 1 collagen. The one-way analysis of variance (ANOVA) and post-hoc Tukey's test were used to compare the groups.</p><p><strong>Results: </strong>The PE-BX group demonstrated promising results, with increased type I collagen levels and reduced expression of inflammatory markers (NF-κB, TLR2 and TNF-α) on days 7 and 30 (p < 0.001). Furthermore, in comparison with the BX group, this material demonstrated significantly higher type I collagen expression on days 7 and 30 (p < 0.01).</p><p><strong>Conclusions: </strong>The composite of a natural propolis extract and a bovine xenograft enhances type I collagen expression and reduces the expression of inflammatory markers. Further research is warranted to explore its potential for alveolar bone preservation.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dynamic fatigue loading on the adaptation and retention of maxillary single dentures reinforced with digitally constructed zirconia and cobalt-chromium frameworks.","authors":"Ghada Mahmoud Elgindy, Hebatallah Mohamed Tarek, Fardos Nabil Rizk, Marwa Ezzat Sabet","doi":"10.17219/dmp/177931","DOIUrl":"https://doi.org/10.17219/dmp/177931","url":null,"abstract":"<p><strong>Background: </strong>The impact of dynamic fatigue loading on denture adaptation and retention is clinically important. Zirconia and cobalt-chromium (Co-Cr) frameworks enhance denture strength, but their performance under cyclic loading remains unclear.</p><p><strong>Objectives: </strong>The aim of the study was to evaluate the adaptation and retention of maxillary single dentures reinforced with digitally constructed Co-Cr or zirconia frameworks after a simulated 1 year of function.</p><p><strong>Material and methods: </strong>Three types of maxillary single dentures were constructed on maxillary master casts. Group A included maxillary dentures without reinforcement (control group), whereas groups B and C encompassed maxillary dentures reinforced with digitally designed and milled Co-Cr and zirconia frameworks, respectively (n = 13/group). Acrylic resin was conventionally processed on stone casts. Initial adaptation was evaluated for each denture using Geomagic software, which allowed for accurate matching and superimposition of the scanned master cast with the standard tessellation language (STL) file of the scanned fitting denture surface obtained with the use of a desktop scanner. Initial retention was evaluated using a universal testing machine. Dynamic fatigue loading of 50 N at a speed of 60 mm/s was applied in the chewing simulator, after which final adaptation and retention values were recorded. Paired t-tests were conducted to compare results within each group, and one-way analysis of variance (ANOVA) was performed to compare the 3 groups. When ANOVA revealed significant differences, Tukey's honestly significant difference (HSD) test was used for post hoc pairwise comparisons. The significance level was set at p ≤ 0.05.</p><p><strong>Results: </strong>Group C showed the most favorable adaptation after dynamic fatigue loading (0.31 ±0.04 mm), while group B exhibited the least favorable adaptation (0.61 ±0.08 mm). Regarding retention, group C demonstrated the highest values (20.04 ±0.96 N), while group B exhibited the lowest values (9.32 ±0.78 N). Significant differences were observed among the 3 groups for both adaptation and retention.</p><p><strong>Conclusions: </strong>Maxillary single dentures reinforced with zirconia frameworks may represent a successful alternative to Co-Cr frameworks for prosthetic rehabilitation using digital technology.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and antibiofilm activities of neem and myrrh extracts against Enterococcus faecalis in dentin specimens.","authors":"Shimaa Rifaat, Hatem Mustafa Abuohashish, Rabab Alsaleh, Maha Alqahtani, Noor Aloraifi, Jood Aljarbaua, Abdullah Albeladi, Youmna Almajed, Faisal Alharamlah, Doaa Aleraky","doi":"10.17219/dmp/197325","DOIUrl":"https://doi.org/10.17219/dmp/197325","url":null,"abstract":"<p><strong>Background: </strong>Enterococcus faecalis is a primary oral pathogen responsible for root canal treatment failures. The clinical use of conventional root canal disinfectants may be associated with cytotoxicity and other unfavorable effects.</p><p><strong>Objectives: </strong>The aim of the study was to assess and compare the antimicrobial effect of Azadirachta indica (neem) and Commiphora molmol (myrrh) extracts against E. faecalis biofilm in dentin samples.</p><p><strong>Material and methods: </strong>The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of neem and myrrh extracts against E. faecalis (ATCC 29212) were determined. Human dentin discs were prepared and inoculated with E. faecalis for 21 days. The antibiofilm activities of neem and myrrh extracts were compared using colony forming unit (CFU) assays, cell proliferation assays and live/dead fluorescence microscopy. A 5.25% sodium hypochlorite (NaOCl) solution and 2% chlorhexidine (CHX) were used as standard disinfectants.</p><p><strong>Results: </strong>The MIC values for the neem and myrrh extracts were 0.0312 mg/mL and 0.0156 mg/mL, respectively, while the MBC values were 0.0625 mg/mL and 0.0312 mg/mL, respectively. The CFU assay revealed a significant decrease in bacterial counts in the neem and myrrh groups. Similarly, cell counting kit-8 (CCK-8) assays demonstrated decreased bacterial viability in the neem and myrrh groups compared with the phosphate-buffered saline (PBS) group. Furthermore, live/dead fluorescence microscopy confirmed the antibacterial effects of both extracts against E. faecalis.</p><p><strong>Conclusions: </strong>Myrrh and neem extracts showed promising antimicrobial potential as root canal disinfectants. These natural agents may serve as effective alternatives to conventional chemical endodontic irrigants.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}