Dental and Medical Problems最新文献

{"title":"Comparative assessment of periodontal conditions between bilateral cleft lip and palate (BCLP) and unilateral cleft lip and palate (UCLP): A systematic review and meta-analysis.","authors":"Jitesh Wadhwa, Alpa Gupta, Puneet Batra","doi":"10.17219/dmp/171896","DOIUrl":"https://doi.org/10.17219/dmp/171896","url":null,"abstract":"<p><p>The existing literature on the periodontal condition in different cleft types is inconclusive and has yielded conflicting results. Therefore, the aim of this systematic review and meta-analysis is to assess and compare the oral health needs of children with bilateral cleft lip and palate (BCLP) with those of children with unilateral cleft lip and palate (UCLP).Six electronic databases were thoroughly searched for articles published up to June 2022 that directly compared the periodontal condition of BCLP patients with that of UCLP patients. A meta-analysis was conducted using the random-effects model with inverse variance weighting. The literature search yielded 858 articles, out of which 58 studies were selected for a full-text review. Finally, 5 articles, which compared 86 BCLP individuals with 132 UCLP patients across 3 continents, were evaluated. The selected papers compared gingival and periodontal parameters, including the plaque index (PI), the gingival index (GI), periodontal probing depth (PPD), and clinical attachment loss (CAL).The meta-analysis revealed a significant difference in CAL on the facial side in BCLP individuals (mean difference: -0.44, 95% confidence interval (CI): 0.27-0.61, Z = 5.07, p < 0.0001). The remaining parameters did not reveal any significant differences between the 2 groups.In light of the established correlation between cleft lip and palate morbidity and surgical interventions on gingival and periodontal health, these factors must be incorporated into treatment planning.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal and dental changes after bone-borne versus tooth-borne surgically assisted rapid palatal expansion in subjects with maxillary transverse deficiency: A systematic review and meta-analysis.","authors":"Leelan Kanwal, Hafsa Qabool, Wafa Idrees, Rashna Hoshang Sukhia, Mubassar Fida","doi":"10.17219/dmp/171393","DOIUrl":"https://doi.org/10.17219/dmp/171393","url":null,"abstract":"<p><p>The objective of the study was to determine the difference in skeletal and dental effects of bone-borne (BB) versus tooth-borne (TB) surgically assisted rapid palatal expansion (SARPE) in subjects with maxillary transverse deficiency (MTD).The present review included randomized controlled trials (RCTs), non-RCTs and cohort studies. A systematic search was conducted in online databases (i.e., PubMed®, Dentistry & Oral Sciences Source, CINAHL Plus, and Cochrane Central Register of Controlled Trials (CENTRAL)) for articles published up to January 2023. The outcome was estimated using the weighted average difference and 95% confidence intervals (CIs). The heterogeneity of the studies was assessed using Cochran's heterogeneity test (I2 test). The metaanalysis was conducted using the RevMan software, v. 5.3.5.22.The qualitative and quantitative synthesis incorporated 7 articles that satisfied the inclusion criteria. The skeletal and dental expansion was assessed preand post-expansion in 249 patients who underwent SARPE with BB and TB appliances. Five studies were included in the meta-analysis to measure skeletal expansion in the first premolar and first molar regions. The analysis revealed no statistically significant differences between the study groups (mean difference: -0.16; 95% CI: -0.34, 0.67). To measure dental expansion, 7 studies were included in the meta-analysis, and no significant differences were observed between them (mean difference: -0.29; 95% CI: -0.77, 0.19).This systematic review and meta-analysis revealed no differences in skeletal and dental expansion in patients who underwent SARPE with BB and TB appliances.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tooth bleaching in patients with fixed orthodontic appliances: A systematic review.","authors":"Gustavo Teodoro Costa Lizarelli, Rafaela Manente, Aline Souza-Gabriel, Silmara Aparecida Milori Corona","doi":"10.17219/dmp/171606","DOIUrl":"10.17219/dmp/171606","url":null,"abstract":"<p><p>Patients seeking a harmonious smile that combines satisfactory aesthetics and stable occlusion raise questions about the concomitant association of treatments. This systematic review aimed to answer the following question: \"Does the application of hydrogen peroxide on teeth with fixed metallic orthodontic brackets interfere with the dental bleaching effect?\". The PICOS (Population, Intervention, Comparison, Outcome, and Study design) strategy and the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines were adhered to during the review process. A comprehensive search strategy was implemented, encompassing the PubMed®, Web of Science, Scopus, Embase, and LILACS databases. The risk of bias was assessed using the Cochrane risk of bias (RoB) 2.0 tool. The initial search yielded 51 articles, which were then filtered to remove duplicates. The titles and abstracts of 26 studies were subsequently reviewed. Eleven articles were selected for full-text reading, and after applying the eligibility criteria, 3 studies were included for qualitative analysis. Two studies were classified as having a low risk of bias, while 1 study was classified as having some concerns. The bleaching was satisfactory with 8% and 10% hydrogen peroxide (HP) applied for 45 min daily over 10 days, even with fixed orthodontic brackets. However, a single study that employed the in-office technique (38% HP) demonstrated that fixed orthodontic appliances influenced external tooth bleaching. Tooth bleaching using the home bleaching technique yielded effective results when performed alongside orthodontic treatment.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anterior Stafne bone defect: Literature review and a case series.","authors":"Dominika Kopciuch, Marzena Dominiak, Ingrid Różyło-Kalinowska, Paweł Kubasiewicz-Ross","doi":"10.17219/dmp/188781","DOIUrl":"https://doi.org/10.17219/dmp/188781","url":null,"abstract":"<p><p>The study objective was to review the literature and to present 3 cases of the anterior Stafne bone defect (SBD). The electronic databases - MEDLINE via PubMed and Google Scholar - were searched by 2 independent authors, who retrieved 20 articles concerning this pathology. The Stafne bone defect is an asymptomatic bone lesion, diagnosed mostly incidentally through radiological imaging, typically located in the lateral section of the mandible. The anterior SBD is exceedingly rarely observed. So far, less than 40 cases have been described. The hypothesis of the formation of a bone cavity in connection with the sublingual salivary gland has not been confirmed in the literature, considering other tissue structures present within the lesion, including lymphoid or adipose tissues. The anterior variant of SBD can be mistaken for other lesions, considering its atypical location and lower incidence rate. In most cases, it does not require any treatment and the 'wait-and-see' strategy is adopted. In the present study, 2 cases of twochamber and 1 case of single-chamber anterior SBDs were presented. Their course was asymptomatic; however, in 2 cases, increased tension of the suprahyoid muscles on physical examination was reported. The cone-beam computed tomography (CBCT) imaging was employed in each case. There was no need for biopsy, and the monitoring of the lesion was established in each reported case.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of potential risk factors for the development of medication-related osteonecrosis of the jaw.","authors":"Monika Bełch, Grażyna Wyszyńska-Pawelec, Mariusz Szuta, Justyna Hajto-Bryk, Piotr Michalak, Jan Zapała, Joanna Zarzecka","doi":"10.17219/dmp/195758","DOIUrl":"https://doi.org/10.17219/dmp/195758","url":null,"abstract":"<p><strong>Background: </strong>Medication-related osteonecrosis of the jaw (MRONJ) is an undesirable consequence of the action of drugs, involving the exposure of bones in a patient not exposed to ionizing radiation in the head and neck area. The occurrence of MRONJ is associated with therapy with anti-resorptive drugs (bisphosphonates), receptor activator of nuclear factor-kappa B ligand (RANKL) drugs, e.g., denosumab, and anti-angiogenic drugs that are vascular endothelial growth factor (VEGF) inhibitors.</p><p><strong>Objectives: </strong>The aim of the present study was to identify risk factors for the development of MRONJ.</p><p><strong>Material and methods: </strong>The medical records of patients hospitalized in the years 2015-2022 in the Department of Maxillofacial Surgery of the Ludwik Rydygier Specialist Hospital in Krakow and the Clinical Department of Maxillofacial Surgery of University Hospital in Krakow, Poland, were retrospectively analyzed. The study included patients treated for MRONJ in the maxilla and/or the mandible with a history of past bisphosphonate therapy. Patients with symptoms of osteonecrosis after radiotherapy of the head and neck region were excluded from the study. The patients' demographic data, comorbidities, the initial disease treated with bisphosphonates, the route of drug administration, the type of causative dental surgery, the area of necrosis, and the MRONJ class according to the American Association of Oral and Maxillofacial Surgeons (AAOMS) criteria were analyzed.</p><p><strong>Results: </strong>The investigated group consisted of 29 females and 14 males. Common comorbidities were anemia, diabetes mellitus (DM) and hypertension. Bisphosphonates were used in 30 patients (69.8%) treated for cancer, in 10 patients (23.3%) treated for osteoporosis and in 2 patients (4.7%) treated for osteopenia. In the majority of cases (n = 19; 44.2%), bisphosphonates were administrated intravenously. Medication-related osteonecrosis of the jaw was diagnosed in the mandible in 25 cases (58.1%) and in 18 (41.9%) - in the maxilla. In 14 patients (32.6%), necrosis was initiated by a dental procedure, most often tooth extraction.</p><p><strong>Conclusions: </strong>Risk factors for the development of MRONJ in patients treated with bisphosphonates include the intravenous route of drug administration, past intraoral surgery, female gender, and senior age.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of heartfulness meditation in the management of recurrent aphthous ulcers.","authors":"Madhulika Yadav, Neha Rathore, Ashish Katiyar, Ravish Ahuja, Amogh Tanwar, Sahana Shivkumar, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.17219/dmp/168912","DOIUrl":"https://doi.org/10.17219/dmp/168912","url":null,"abstract":"<p><strong>Background: </strong>Recurrent aphthous ulcers (RAUs) are the most prevalent form of painful oral mucosal disorder. Conventional treatment for RAUs involves the administration of tetracycline, which has been shown to alleviate clinical signs and symptoms. However, this medication has been associated with a number of adverse effects. Heartfulness meditation (HM) has been shown to facilitate natural healing of ulcers without complications by developing a compassionate connection with oneself.</p><p><strong>Objectives: </strong>The aim of the study was to evaluate the effectiveness of HM in the management of RAUs and to compare its effects with those of conventional treatment.</p><p><strong>Material and methods: </strong>A parallel study was conducted among 54 patients affected with RAUs. The patients were divided into 3 groups: group 1 - HM; group 2 - tetracycline treatment; and group 3 - combination of tetracycline treatment and HM. The evaluated outcomes included pain levels and the duration of ulceration. One-way analysis of variance (ANOVA) and paired t-test were applied to identify significant differences between the groups.</p><p><strong>Results: </strong>A substantial decrease in pain intensity was observed during HM. The group that engaged in meditation alongside tetracycline treatment exhibited the lowest pain scores. At follow-up intervals of 3, 5 and 7 days, pain levels between the tetracycline group and the HM group were similar. The group that received a combination of tetracycline treatment and HM demonstrated the shortest duration of ulcers (7.167 ±0.383 days).</p><p><strong>Conclusions: </strong>The study concluded that when HM was practiced in addition to tetracycline treatment, it demonstrated superior efficacy in reducing pain at the shortest time. Furthermore, the study noted a reduction in the duration of ulcers when HM was used in conjunction with tetracycline, as compared to the practice of HM alone.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of EDTA, fumaric acid, and the chitosan solutions prepared with distilled water and acetic acid on dentin microhardness and tubular penetration: An in vitro study.","authors":"Esma Sariçam, Hanife Altınışık, Neslihan Bulak, Berna Güven, Sabiha Zeynep Yurdagül, Gülin Amasya","doi":"10.17219/dmp/167467","DOIUrl":"https://doi.org/10.17219/dmp/167467","url":null,"abstract":"<p><strong>Background: </strong>Chitosan prepared with acetic acid is commonly used as an endodontic irrigant. However, the chitosan solution prepared with distilled water has not been evaluated for endodontic usage.</p><p><strong>Objectives: </strong>The present study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA), fumaric acid, and the chitosan solutions prepared with distilled water (C-DW) and acetic acid (C-AA) on dentin microhardness and dentinal tubule penetration.</p><p><strong>Material and methods: </strong>Eighty maxillary central incisors were endodontically instrumented and randomly divided into 2 main groups (n = 40) for the evaluation of dentin microhardness and tubular penetration, with 4 subgroups in each main group (n = 10) according to the final irrigation solutions used (EDTA, fumaric acid, C-DW, and C-AA). The C-AA solution was prepared by diluting medium-molecularweight chitosan in acetic acid. The C-DW solution was prepared with distilled water and chitosan ammonium salts, which were synthesized using trichloroacetic acid and low-molecular-weight chitosan. After irrigation, the roots were sectioned horizontally 2 mm (the apical third) and 5 mm (the middle third) from the apex. The microhardness measurements were taken at depths of 500 μm and 1,000 μm from the canal lumen. The sections were examined for tubular penetration using confocal laser scanning microscopy. The data was analyzed using the analysis of variance (ANOVA), with a significance level set at p < 0.05.</p><p><strong>Results: </strong>The microhardness values were statistically similar at either depth for each third (p > 0.05), except for the 1,000-μm depth in the apical third, where the use of the C-AA solution resulted in lower microhardness as compared to fumaric acid (p < 0.05). No significant differences were observed in tubular penetration with regard to each third (p > 0.05).</p><p><strong>Conclusions: </strong>All solutions showed a similar penetration ability in each third. At the 1,000-μm depth in the apical third, the fumaric acid solution provided a higher microhardness value than the C-AA solution.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of selected parameters of reparative dentin after direct pulp capping with MTA Repair HP in human teeth, using CBCT and micro-CT.","authors":"Mirona Palczewska-Komsa, Adam Puszkarz, Bartosz Dalewski, Kinga Kaczor-Wiankowska, Ewa Sobolewska, Łukasz Pałka, Magdalena Gębska, Alicja Nowicka","doi":"10.17219/dmp/189747","DOIUrl":"https://doi.org/10.17219/dmp/189747","url":null,"abstract":"<p><strong>Background: </strong>The biomimetic regeneration of the pulp-dentin complex is a modern approach involving the application of a bioactive substance. Bioactive substances (e.g., cement or stem cells) have been reported to be able to control the signaling and differentiation of the pulp cells, thus limiting the inflammatory reactions and enabling the repair and regeneration of tissues. One of such materials is MTA Repair HP (MTA HP) - a type of mineral trioxide aggregate (MTA) material characterized by high plasticity, composed of hydrophilic particles of mineral oxides.</p><p><strong>Objectives: </strong>The aim of the present study was to analyze the reaction of the pulp-dentin complex of human teeth to MTA HP using 2 radiological techniques - cone-beam computed tomography (CBCT) and micro-computed tomography (micro-CT).</p><p><strong>Material and methods: </strong>In the study, 6 caries-free, intact maxillary and mandibular third molars or premolars from 6 patients aged 30-37 years were analyzed. The teeth were scheduled to be extracted for orthodontic or surgical reasons.</p><p><strong>Results: </strong>No statistically significant differences in the examined parameters of the tertiary dentin were observed in the CBCT and micro-CT images (p < 0.05).</p><p><strong>Conclusions: </strong>The CBCT technique, similarly to micro-CT, proved to be effective in identifying and assessing the tertiary dentin. Furthermore, CBCT has the advantage of usability in clinical settings. Knowledge on the parameters of reparative dentin identified with radiological techniques is still insufficient, and further research is recommended.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of endodontic retreatment by conventional therapy compared to combined therapy with an Er:YAG laser and photobiomodulation: A randomized clinical trial.","authors":"Ilona Kolberg-Babrzyńska, Kinga Grzech-Leśniak, Jan Kiryk, Marzena Dominiak, Jacek Matys","doi":"10.17219/dmp/188864","DOIUrl":"https://doi.org/10.17219/dmp/188864","url":null,"abstract":"<p><strong>Background: </strong>The success of endodontic retreatment relies on the effective elimination of pathogenic microflora from the root canal.</p><p><strong>Objectives: </strong>The study aimed to investigate the effects of an erbium-doped yttrium-aluminum-garnet (Er:YAG) laser and a 635-nm laser on the healing of asymptomatic chronic periapical lesions (PLs) in endodontically treated teeth and the reduction of postoperative pain.</p><p><strong>Material and methods: </strong>Forty patients with PLs in mandibular molars were referred for root canal retreatment (RCR). Conventional chemo-mechanical endodontic treatment was conducted in the control group (G1; n = 20). In the test group (G2; n = 20), in addition to conventional chemo-mechanical treatment, Er:YAG laser-activated irrigation (LAI) with 2% NaOCl and 17% EDTA was performed. The laser parameters were as follows: 50 mJ; 25 Hz; 1 W; 300 μs; a tip diameter of 300 μm; fluence of 71.4 J/cm2; and power density of 1,428.6 W/cm2. Subsequently, the canals were filled with thermo-condensed guttapercha, using the AH Plus sealer. In group G2, additional photobiomodulation (PBM) with a wavelength of 635 nm (400 mW, 5 s per point, a dose per point: 2 J, a dose per square centimeter: 4 J, an applicator diameter of 8 mm) was applied, with 2 application points at the apex level, administered over 4 sessions - on the treatment day, and after 24 h, 48 h and 96 h. Endodontic lesion remission was assessed by measuring the PL size with the use of cone-beam computed tomography (CBCT) at 6 and 12 months postoperatively. Postoperative pain was evaluated using the visual analog scale (VAS) after 1, 2 and 4 days.</p><p><strong>Results: </strong>The study results demonstrated a statistically significant decrease in the mean PL size at 6 months postoperatively in the test group (mean PL size: 1.55 ±0.51 mm) as compared to the control group (mean PL size: 1.95 ±0.71 mm) (p < 0.05). In the test group, postoperative pain on VAS was significantly lower after the procedure (p < 0.05).</p><p><strong>Conclusions: </strong>The application of Er:YAG and 635-nm diode lasers improved PL healing and decreased postoperative pain.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of the effect of Er:YAG laser with a fractional PS04 handpiece on microbial biofilm survival.","authors":"Zuzanna Grzech-Leśniak, Jakub Pyrkosz, Jagoda Szwach, Martyna Lelonkiewicz, Magdalena Pajączkowska, Joanna Nowicka, Jacek Matys, Kinga Grzech-Leśniak","doi":"10.17219/dmp/201941","DOIUrl":"https://doi.org/10.17219/dmp/201941","url":null,"abstract":"<p><strong>Background: </strong>The oral microbiota consists of a diverse range of microorganisms, with Streptococcus spp. and Candida spp. frequently coexisting in oral infections.</p><p><strong>Objectives: </strong>The aim of the study was to investigate the impact of Er:YAG (erbium-doped yttrium aluminum garnet) laser therapy, utilizing the PS04 fractional beam, on the in vitro growth and biofilm formation of clinical strains of Candida albicans, Candida glabrata and Streptococcus mutans.</p><p><strong>Material and methods: </strong>Singleand dual-species planktonic cultures and biofilms were exposed to an Er:YAG laser using a fractional PS04 handpiece. The effects of the laser were evaluated immediately after irradiation and 24 h post-irradiation by measuring colony-forming units per milliliter (CFU/mL). Biofilm biomass (singleand dual-species) was quantified using the crystal violet staining method. The study tested 2 sets of laser parameters: group 1 (T1): 1.5 W, 10 Hz, 30 s, 0.4 J/cm2, irradiance: 3.9 W/cm2; and group 2 (T2): 6.15 W, 10 Hz, 30 s, 1.6 J/cm2, irradiance: 16 W/cm2. Non-irradiated samples served as controls. The parameters were selected based on their frequent clinical use for snoring treatment and facial rejuvenation.</p><p><strong>Results: </strong>Candida albicans exhibited a significantly greater reduction under T2 settings in comparison to T1 (85.3 ±1.2% vs. 43.9 ±4.5%, respectively; p = 0.006) within single-species biofilms. For C. glabrata, a significant reduction was observed under T1 parameters (69.8 ±14.9%). Furthermore, S. mutans demonstrated a significantly higher reduction at T2 settings (97.1 ±0.6%) compared to T1 settings (81.1 ±19.6%), with statistically significant differences noted between S. mutans and both C. albicans and C. glabrata under T1, as well as between S. mutans and C. glabrata under T2. In dual-species biofilms, T2 fluence led to greater reductions in C. glabrata, S. mutans and C. albicans in mixed cultures (p < 0.05).</p><p><strong>Conclusions: </strong>The Er:YAG laser, when used in conjunction with the PS04 handpiece, demonstrated a substantial reduction in the biofilms of C. albicans and C. glabrata. Higher fluence maintained reductions over a 24-h period, particularly in the case of C. glabrata and S. mutans. This study highlights the antifungal potential of low-fluence laser settings that are commonly used in facial aesthetic procedures and snoring treatment.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}