Current Trends in Biotechnology and Pharmacy最新文献_第8页

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.39

Mandara V Mahadevaiah, Prasanna K. Santhekadur

引用次数: 1

Development of in-house indirect enzyme linked immunosorbent assay (iELISA) for detection of Salmonella enteritidis dpecific antibodies in poultry 内部间接酶联免疫吸附试验（iELISA）检测家禽肠炎沙门氏菌特异性抗体的建立

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.26

Navaneetha P. Navaneetha, M. Anil, A. V. Kumar, Manasa Manasa, P. Reddy, S. Sudhakar, Rathnagiri P. Rathnagiri

{"title":"Development of in-house indirect enzyme linked immunosorbent assay (iELISA) for detection of Salmonella enteritidis dpecific antibodies in poultry","authors":"Navaneetha P. Navaneetha, M. Anil, A. V. Kumar, Manasa Manasa, P. Reddy, S. Sudhakar, Rathnagiri P. Rathnagiri","doi":"10.5530/CTBP.2020.3.26","DOIUrl":"https://doi.org/10.5530/CTBP.2020.3.26","url":null,"abstract":"Salmonella enteritidis is a most important pathogenic bacterium of avian and mammals. Salmonella Enteritidis is the main cause of Salmonellosis in poultry flocks. S. enteritidis majorly infects the chicks, eggs and vertically transmitted to their off springs. The majority of the food infections to the humans are caused by salmonella by eating chicken meat and eggs. Monitoring of poultry farms with the bacteriological methods were time consuming and labour intensive process. The present study was development an in-house indirect enzyme linked immunesorbent assay (iELISA) for the detection of antibodies against Salmonella enteritidis in chicken serum samples. For detection of antibodies, Salmonella enteritidis LPS was used as antigen and rabbit anti chicken IgG HRP was used as the secondary antibody to detect antibodies against Salmonella enteritidis . The developed in-house ELISA was compared with the Rapid plate agglutination test. The purified LPS antigen 200ng/well, test sample serum at a dilution of 1:100 and rabbit anti chicken IgG HRP 1:10000 were used as optimal concentration of the assay and OD was measured at 450nm. A total of 1020 chicken serum samples were collected and performed the assay along with known Positive and negative controls. Out of these samples 592 and 566 samples were seropositive with iELISA and RPA respectively. Out of 1020 samples 58% samples shown positive immune response with iELISA and 55.6% samples were shown positive immune response to Rapid plate agglutination assay. The major prevalence of SE antibodies against SE antigen were shown in 20-25 weeks birds was 65.5%.The findings suggested that an in-house indirect ELISA based on S.enteritidis LPS can be a useful as a rapid and sensitive assay for the detection of antibodies to S.enteritidis and can be best assay for regular monitoring of Salmonella Enteritidis infection in flocks.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42803322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced L-lysine production through chemical mutagenesis in Corynebacterium glutamicum MTCC 25069 通过化学诱变提高谷氨酸棒状杆菌MTCC 25069的l -赖氨酸产量

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.29

Ramesh Malothu

引用次数: 0

Formulation of pullulan/plasticizer blended films for their physical and biodegradability studies 普鲁兰/增塑剂共混膜的配方及其物理和生物降解性研究

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.27

V. S. R. K. Ganduri, M. Ushakiranmayi, K. Rao, S. Poda

{"title":"Formulation of pullulan/plasticizer blended films for their physical and biodegradability studies","authors":"V. S. R. K. Ganduri, M. Ushakiranmayi, K. Rao, S. Poda","doi":"10.5530/CTBP.2020.3.27","DOIUrl":"https://doi.org/10.5530/CTBP.2020.3.27","url":null,"abstract":"The present investigation utilizes the eco-efficient pullulan polysaccharide as film forming biopolymer. Pullulan-based edible films offer good physical, thermal and mechanical properties which enable them to use in shelf-life preservation of fresh produce. Blends of other film forming polysaccharides, plasticizers and an antioxidant with pullulan (Pu) solution were prepared in order to determine physical and optical parameters of those films. The morphological and biodegradable studies were attempted to identify the changes on the films’ surfaces. The films made from (only) pullulan (10Pu), pullulan composited with sodium alginate(10Pu_0.5SA), gelatin (10Pu_0.5G), polyethylene glycol (10Pu_0.5PG), calcium chloride and lemon juice (10Pu_1CC_2L) resulted heavier film densities, higher whiteness indexes and lower total color difference values.All the films were tested for their biodegradability in soil, where visual changes were appreciated after 15 days, partial and complete degradation took place at the end of 34 days and 53 days, respectively. Thus, these pullulan blended films could be a better replacement for synthetic films towards environmental problems.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48809702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Green synthesis of silver nanoparticles from fractionated Annona reticulata leaf extract in different solvents and analysis of its antioxidant and antibacterial activity 网状番荔枝叶提取物在不同溶剂中的绿色合成及其抗氧化抗菌活性分析

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.30

Sugunakar Sugunakar Yj, P ChandramatiShankar

引用次数: 1

Generation of gamma irradiated mutagenized population in Solanum lycopersicum CV. Arka Vikas 番茄茄γ辐照诱变群体的产生。Arka维

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.28

P. Prashanth

{"title":"Generation of gamma irradiated mutagenized population in Solanum lycopersicum CV. Arka Vikas","authors":"P. Prashanth","doi":"10.5530/CTBP.2020.3.28","DOIUrl":"https://doi.org/10.5530/CTBP.2020.3.28","url":null,"abstract":"Natural and induced mutants are the primary resource for understanding the functions of the genes. The study of lethal dose (LD) and development of 300Gy, 400Gy, and 500Gy gamma irradiation population in Solanum lycopersicum L. cv. ArkaVikas was carriedout in the present investigation. The highest germination percentage was recorded in control (98.53%). The germination percentage reduction, seedling height decline, and decreased pollen fertility in M1 generation was noted with the increasing gamma radiation dose. The 300Gy irradiation caused 41.73% seed germination reduction and was considered an appropriate irradiation dose to develop a mutagenized population. A total of 3000 seeds have been irradiated with 300Gy of gamma rays and 1,748 (58.26%) M1 mutagenized plants have survived and out of these 1,185 viable fertile plants were noted. In M1 generation two chlorophyll mutants have been identified. Six plants from each M2 line were screened based on morphological alterations and six putative mutants (0.51%) were identified. The identified mutant lines displayed a varied range of morphological variations that include altered chlorophyll content, elongated fruits, and orange color fruit.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":"14 1","pages":"271-278"},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46870809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A simple and novel sample preparation approach for effective characterization of antibody low molecular weight impurities by CE-SDS method CE-SDS法是一种简单、新颖的样品制备方法，可有效地表征抗体低分子量杂质

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.32

B. Bheemareddy, P. Iyer, Kranthi Vemparala, V. R. Dirisala

{"title":"A simple and novel sample preparation approach for effective characterization of antibody low molecular weight impurities by CE-SDS method","authors":"B. Bheemareddy, P. Iyer, Kranthi Vemparala, V. R. Dirisala","doi":"10.5530/CTBP.2020.3.32","DOIUrl":"https://doi.org/10.5530/CTBP.2020.3.32","url":null,"abstract":"Effect of sample buffer composition and pH on LMW impurities analysis Abstract Product and process related impurities of biopharmaceuticals have serious implications on product safety and efficacy in clinical use. Low molecular weight (LMW) impurities are generated during process and stability studies are routinely analyzed using non-reducing capillary electrophoresis with SDS during different stages of product development and release. The current sample processing methodology with heat denaturation is known to induce fragmentation and interfere with the LMW impurity analysis. In this study, we compared different sample processing buffers with different compositions and pH and finally found a solution to the problem of sample artifacts generated during heat denaturation step of sample processing which interferes with the LMW impurity analysis by CE-SDS method. We compared three sample buffers (100 mM Tris-Cl, 25mM Citrate and 25mM citrate with Urea) in for their ability to maintain product integrity during sample processing at different pH and temperatures in the non-reducing CE-SDS analysis. This study suggests that, the sample processing with 25mM citrate with 8M Urea sample processing buffer does not require heat denaturation at higher temperatures and hence is the most appropriate buffer for sample processing in the LMW impurity analysis. The 25mM Citrate + 8M Urea buffer has shown better drug product stability and integrity compared to other buffers. Hence, we recommend the 25mM Citrate buffer with 8M Urea for sample processing in LMW impurity analysis by CE-SDS method.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":"14 1","pages":"311-318"},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49203940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of a haloalkaline pectinase from Halomonas pantellerinsis strain SSL8 isolated from Sambhar lake, Rajasthan 拉贾斯坦邦Sambhar湖盐单胞菌SSL8盐碱性果胶酶的制备及特性研究

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.33

M. N. Cherekar, A. Pathak

引用次数: 0

Anticancer potential of D-limonene and hispolon against colon cancer cell lines D-柠檬烯和hispolon对结肠癌细胞株的抗癌作用

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.31

K. C. Sekhar, Rajanikanth A. Rajanikanth, Md Nazneen Bobby, J. R. Kanala

引用次数: 1

Morphological and biochemical characterization of fluorescent Pseudomonads from groundnut rhizosphere 花生根际荧光假单胞菌的形态和生化特性

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI: 10.5530/CTBP.2020.3.35

Padma Gurucharan Jogi, L.Nirmala Jyothi, K. Kumar, P. O. Basha, E. Reddy

{"title":"Morphological and biochemical characterization of fluorescent Pseudomonads from groundnut rhizosphere","authors":"Padma Gurucharan Jogi, L.Nirmala Jyothi, K. Kumar, P. O. Basha, E. Reddy","doi":"10.5530/CTBP.2020.3.35","DOIUrl":"https://doi.org/10.5530/CTBP.2020.3.35","url":null,"abstract":"Groundnut is an important grain legume and oil seed crop grown all over the world. Fluorescent Pseudomonads are beneficial bacteria that inhabit the root zone of plants and increase the growth of plants by a wide variety of mechanisms. In our studies,, we have assessed the morphological and biochemical characteristics of 24 fluorescent pseudomonads isolated from groundnut rhizosphere collected from Rayalaseema region of Andhra Pradesh. All the Pseudomonas fluorescens (Pf) strains showed development of fluorescent pigments under UV light. Morphological studies indicated that they have displayed (i) rod-shaped, (ii) smooth shiny surface, (iii) gram-negative reaction (iv) motility and (v) growth even at 410C. None of the strains were positive for the indole, and voges-proskauer test. All strains were positive for oxidase, citrate, catalase, and gelatin liquefaction test; 58% were starch hydrolysis-positive, 29.16% were methyl red-positive and 62.5% were H2S-positive. The present study proved that these Pf strains have potential plant growth-promoting activities. Our future studies are directed in establishing the plant growth-promoting effects of these Pf strains under Greenhouse and field conditions on groundnut.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":"14 1","pages":"340-346"},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41905825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0