Current Trends in Biotechnology and Pharmacy最新文献_第7页

Antibacterial and Antioxidant Activities of Aqueous Extract of Soapnuts (Sapindus mukorossi) 皂子水提物的抗菌和抗氧化活性研究

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.40

Aruna Jyothi Kora

{"title":"Antibacterial and Antioxidant Activities of Aqueous Extract of Soapnuts (Sapindus mukorossi)","authors":"Aruna Jyothi Kora","doi":"10.5530/ctbp.2020.4.40","DOIUrl":"https://doi.org/10.5530/ctbp.2020.4.40","url":null,"abstract":"As an alternative to chemical surfactants, the biosurfactants obtained from plants are renewable, biocompatible, biodegradable, less toxic and less expensive. In the present report, the aqueous extract prepared from the pericarps of soapnuts fruits is employed, which are known to exhibit a myriad of biological properties. The extract was characterized using Fourier transform infrared spectroscopy (FTIR) and zeta analyzer for identifying the functional groups and surface charge, respectively. The extract showed abundance of saponins, triterpenoids, flavonoids and negative charge of –8.9 mV. The free radical scavenging and antibacterial activities of the extract were evaluated with DPPH scavenging and well diffusion assays. The DPPH scavenging (%) increased with an increase in extract concentration and showed a significant radical scavenging potential of 85.3% at a concentration of 250 μg/mL. The extract didn’t show antibacterial action on Gram–negative bacteria at the selected concentrations. But, it demonstrated significant inhibitory action on Gram–positive bacteria; Bacillus subtilis and Micrococcus luteus with inhibition zones of 4.0 mm and 12.5 mm at 43.75 mg of crude saponins, respectively. Thus, the green extract used in the present study finds its application as a natural, antibacterial and antioxidant biosurfactant in cosmetic and food industries, as a substitute to chemical surfactants.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46007842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The Implication of a Novel Herbal Formulation in Reversal of Drug-Resistance for Cancer Treatment 一种新型中药制剂逆转癌症耐药性的意义

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.44

U. Ankita, A. RaoGururaj, Rao Rao S.G.A, D. Debjani, A. RaoJyothsna

{"title":"The Implication of a Novel Herbal Formulation in Reversal of Drug-Resistance for Cancer Treatment","authors":"U. Ankita, A. RaoGururaj, Rao Rao S.G.A, D. Debjani, A. RaoJyothsna","doi":"10.5530/ctbp.2020.4.44","DOIUrl":"https://doi.org/10.5530/ctbp.2020.4.44","url":null,"abstract":"Traditional medicines or herbal medicinal compounds are being increasingly considered as useful complementary and alternative treatments for cancer. A large number of in vitro and in vivo studies have reported the beneficial effects of herbal medicines alone and in combination with conventional therapeutics. We developed a polyherbal formulation Swastharakshak® (SR033), exhibiting anti-tumorigenic property. Although, majorly 5-Fluorouracil (5FU) resulted in necrosis of HeLa cells, however in combination with SR033, the majority of cells underwent apoptosis with an increase of 30%, SR033 alone was considered as an experimental control. Moreover, 5FU and PTX resistance were reversed by SR033 with 2.5×103and 0.17×103 fold, respectively, in resistant HeLa-R cells. We observed a 2 fold decrease in free radicals when treated with SR033 as compared to untreated controls; however, 1.2 fold decrease was observed in SR033 + 5FU groups in comparison to 5FU alone.These results demonstrated that SR033 acts synergistically with 5FU, along with chemo-protective and 5FU resistance reversal property. Hence, SR033 is a potential herbal formulation that could be used effectively with 5FU as a combination therapy for cancer.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49596383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational and Experimental Validation of Methotrexate as Staphylococcal - DHFR inhibitor 甲氨蝶呤作为葡萄球菌- DHFR抑制剂的计算和实验验证

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.41

M. Mammen, Archana G. Mohanan, Praveen Kumar

{"title":"Computational and Experimental Validation of Methotrexate as Staphylococcal - DHFR inhibitor","authors":"M. Mammen, Archana G. Mohanan, Praveen Kumar","doi":"10.5530/ctbp.2020.4.41","DOIUrl":"https://doi.org/10.5530/ctbp.2020.4.41","url":null,"abstract":"Dihydrofolate reductase (DHFR) is an enzyme that reduces dihydrofolate to tetrahydrofolate for the denovo synthesis of purines. DHFR is a well-established and classic drug target used in cancer therapy. Several drugs like Methotrexate (MTX), Trimethoprim, Pemetrexed, Pyrimethamine, etc. are known inhibitors of DHFR and presently used to treat cancer patients. Staphylococcus is a major human pathogen and the causative agent of numerous hospital and community-acquired infections. In the present investigation, DHFR of Staphylococcus aureus (PDB Id: 2W9G) was subjected to molecular docking to evaluate whether the anti-cancer drugs, MTX and Pemetrexed strongly bind to the former. The results of molecular docking indicated that MTX and Pemetrexed strongly interact with Staphylococcal DHFR with the binding energy of -8.3kcal/mol and -9.0kcal/mol respectively. To validate the in silicostudies, the antimicrobial property of MTX and pemetrexed was evaluated in clinical strains of S. arlettae and S.sciuriin vitro and the results indicated that MTX but not pemetrexed possessed antimicrobial activity. But the similar antimicrobial effect of the abovementioned drugs was not found in gram-negative bacteria Pseudomonas aeruginosa (ATCC 27853), and E. coli (ATCC 25922). The Minimal Inhibitory Concentration (MIC) of MTX was found to be higher than 2mg/ml for both strains. Even though the MIC values of MTX are high, we propose that structural modification of MTX or its combination with conventional antibiotics may lead to the discovery of the new potential antimicrobial drug.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42706871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Anatomical Characters of Rauwolfia serpentine Benth. ex Kruz. 蛇床草的形态解剖学特征。前Kruz。

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.46

M. K. Vyas

引用次数: 1

Role of IoT and its Adoption in Smart Farming 物联网的作用及其在智能农业中的应用

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.47

Chavali L. N. Chavali, P. Kishore, P. Narayana

引用次数: 1

Cystic Fibrosis in Human - A Review 人类囊性纤维化研究综述

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.48

P. Patel

引用次数: 2

Optimization of Process Parameters for High Yield Production of Exo-Inulinase from Trichoderma asperellum RSBR08 by Using Solid State Fermentation 曲霉木霉RSBR08固体发酵高产产菊粉酶工艺参数优化

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.45

Babul Reddy G, K. Sukumar, G. Swarnabala Swarnabala G

引用次数: 0

Optimization of Polyhydroxybutyrate (PHB) Production by Locally Isolated Bacillus aryabhattai Using Response Surface Methodology 响应面法优化局部分离aryabhattai芽孢杆菌生产聚羟基丁酸酯(PHB

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.37

Neveen M. El-Metwally, R. Allam, M. Shafei, M. Amin, Yasser M Ragab, H. El-Refai, A. E. Khattab

{"title":"Optimization of Polyhydroxybutyrate (PHB) Production by Locally Isolated Bacillus aryabhattai Using Response Surface Methodology","authors":"Neveen M. El-Metwally, R. Allam, M. Shafei, M. Amin, Yasser M Ragab, H. El-Refai, A. E. Khattab","doi":"10.5530/ctbp.2020.4.37","DOIUrl":"https://doi.org/10.5530/ctbp.2020.4.37","url":null,"abstract":"In the present study, an attempt was made to isolate an efficient Polyhydroxybutyrate producing bacterium from soil. A total of 38 different types of bacteria were isolated, out of which 15 were found to be PHB positive, based on the viable colony staining method of screening using Nile red Dye. The isolate (6N) showed maximum PHB production of 0.17 g/L, and PHB produced was confirmedusing NMR.The most potent isolate (6N-NRC) was identified using 16S rRNA, and phylogenetic analysis clearly demonstrated that the strain 6N-NRC is a member of the genus Bacillus and is identified as Bacillus aryabhattai. The culture medium and growth parameters were optimized using one factor at a time, multifactorial experimental design (PlackettBurman and Box-Behnken) and utilization of Beet molasses as cheap and economic carbon source for maximum PHB production was done. Beet molasses (30 g/L) as the carbon and ammonium chloride (0.75 g/L) as the nitrogen source were found to be the best nutritional sources for maximum PHB production. Incubation time period 36h, pH of the medium at 8.0 and temperature of 300C were found to be optimum conditions for obtaining maximum PHB yield of 3.799 g/L.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45545435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A New Approach for Tracing Adulteration of Saffron with Safflower by Universal Barcoding Primers 通用条形码引物溯源藏红花掺假的新方法

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.38

J. Goodarzi, F. Mokhtari, F. Hosseini

引用次数: 2

Autologous Stem Cell Transplantation to Treat Emery- Dreifuss Muscular Dystrophy 自体干细胞移植治疗金刚砂-锥状肌营养不良

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI: 10.5530/ctbp.2020.4.43

Vijay Rajesh Andanamala, N. Sankar

引用次数: 0