曲霉木霉RSBR08固体发酵高产产菊粉酶工艺参数优化

Q3 Pharmacology, Toxicology and Pharmaceutics

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-12-16 DOI:10.5530/ctbp.2020.4.45

Babul Reddy G, K. Sukumar, G. Swarnabala Swarnabala G

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引用次数: 0

摘要

菊粉酶是一种工业应用酶，广泛用于生产超高果糖糖浆和低聚果糖。以成本有效的方式生产菊粉酶是面临的主要挑战。在本研究中，利用固态发酵法生产了具有高外显菊粉酶能力的木霉RSBR08。对固体发酵生产菊粉外酶的工艺参数进行了优化。结果表明，以大蒜为基质，菊粉外酶产量高（199.2±6.3U/gds），水分含量高（228.4±3.4），温度26℃（219.1±5.8），pH值5.0（213.6±4.5），研究了Mn2+和Hg2+对产菊粉酶的影响，其中Ca2+诱导产酶（196.3±5.6），而Hg2＋抑制产酶（37.0±3.3）。

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Optimization of Process Parameters for High Yield Production of Exo-Inulinase from Trichoderma asperellum RSBR08 by Using Solid State Fermentation

Inulinase is an industrially applicable enzyme which is used widely in the production of ultra high fructose syrups and fructo-oligosaccharides. Production of inulinase enzyme in a cost effective manner is the major challenge faced. In the present research study, Trichoderma asperellum RSBR08 which has the capability to produce high exo-inulinase enzyme is produced by using the solid state fermentation. Process parameters for production of exo-inulinase by using solid state fermentation were optimized. Results showed high exo-inulinase production in garlic as substrate (199.2±6.3 U/gds), 45% moisture percentage (228.4±3.4), 26 o C temperature (219.1±5.8) and 5.0 pH (213.6±4.5). Effect of different metals viz. Mg 2+ ,Zn 2+ , K + ,Ca 2+ , Na + ,Mn 2+ and Hg 2+ were studied on the production of exo-inulinase enzyme in which Ca 2+ induced the enzyme production (196.3±5.6) whereas Hg 2+ has inhibited the inulinase production (37.0±3.3). Based on the above results, the solid substrate fermentation parameters optimized can be used industrially.

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来源期刊

Current Trends in Biotechnology and Pharmacy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.