Generation of gamma irradiated mutagenized population in Solanum lycopersicum CV. Arka Vikas

Q3 Pharmacology, Toxicology and Pharmaceutics

Current Trends in Biotechnology and Pharmacy Pub Date : 2020-09-22 DOI:10.5530/CTBP.2020.3.28

P. Prashanth

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引用次数: 1

Abstract

Natural and induced mutants are the primary resource for understanding the functions of the genes. The study of lethal dose (LD) and development of 300Gy, 400Gy, and 500Gy gamma irradiation population in Solanum lycopersicum L. cv. ArkaVikas was carriedout in the present investigation. The highest germination percentage was recorded in control (98.53%). The germination percentage reduction, seedling height decline, and decreased pollen fertility in M1 generation was noted with the increasing gamma radiation dose. The 300Gy irradiation caused 41.73% seed germination reduction and was considered an appropriate irradiation dose to develop a mutagenized population. A total of 3000 seeds have been irradiated with 300Gy of gamma rays and 1,748 (58.26%) M1 mutagenized plants have survived and out of these 1,185 viable fertile plants were noted. In M1 generation two chlorophyll mutants have been identified. Six plants from each M2 line were screened based on morphological alterations and six putative mutants (0.51%) were identified. The identified mutant lines displayed a varied range of morphological variations that include altered chlorophyll content, elongated fruits, and orange color fruit.

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番茄茄γ辐照诱变群体的产生。Arka维

自然和诱导突变体是了解基因功能的主要资源。300Gy、400Gy和500Gy辐照对茄类植物致死剂量的影响及种群发育的研究。在本调查中进行了ArkaVikas。对照发芽率最高，为98.53%。随着γ辐射剂量的增加，M1代的发芽率降低、苗高下降、花粉育性降低。300Gy辐照使种子萌发率降低41.73%，被认为是产生诱变群体的适宜辐照剂量。用300Gy γ射线辐照3000粒种子，1748株(58.26%)M1诱变植株存活，其中1185株可育。在M1代中发现了两个叶绿素突变体。根据形态变异对每个M2系筛选6株，鉴定出6个推定突变体(0.51%)。所鉴定的突变系表现出多种形态变异，包括叶绿素含量改变，果实拉长，果实呈橙色。

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来源期刊

Current Trends in Biotechnology and Pharmacy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.