Cytotechnology最新文献

{"title":"New mechanism of miR-34a-5p in regulating the biological behavior of osteosarcoma by targeting FoxM1.","authors":"Wenxiang Shen, Xiang Liu, Shengdong Wang, Shaowen Du, Liming Cong, Yulong Ma, Kaishan Ye","doi":"10.1007/s10616-025-00758-y","DOIUrl":"https://doi.org/10.1007/s10616-025-00758-y","url":null,"abstract":"<p><p>Osteosarcoma (OS), the most common primary malignant bone tumor in pediatric and adolescent populations, is characterized by significant morbidity and mortality. MicroRNAs (miRNAs) are essential non-coding RNAs that exert pivotal regulatory functions in diverse physiological and pathological processes, including tumorigenesis, disease progression, and drug resistance. The association of miR-34a-5p with osteosarcoma has been documented; However, its underlying mechanisms remain poorly understood.This investigation delineates the impact of miR-34a-5p on the proliferation, invasion, migration, and apoptosis of osteosarcoma cells via in vitro assays, aiming to elucidate the associated mechanisms. Employing up-regulation and knockdown strategies, this study evaluated the roles of miR-34a-5p and FoxM1 in modulating osteosarcoma cell behaviors.These effects were further validated through a rescue experiment, providing robust evidence of the miRNA's impact. Quantitative RT-PCR showed that, compared with normal tissues, miR-34a-5p was significantly downregulated while FoxM1 was markedly upregulated in nine osteosarcoma samples.Increased miR-34a-5p expression attenuated proliferation, migration, and invasion in MG-63 and U2OS cell lines, while enhancing apoptosis.Bioinformatic analyses and dual luciferase assays identified FoxM1 as a downstream target of miR-34a-5p, a finding corroborated by quantitative RT-PCR and Western blotting, which confirmed the negative regulation of FoxM1 by miR-34a-5p.Additionally, FoxM1 knockdown reduced tumor cell proliferation, migration, and invasion, concurrently promoting apoptosis; co-inhibition of miR-34a-5p and FoxM1 partially mitigated these effects. This study demonstrates that miR-34a-5p significantly inhibits osteosarcoma cell proliferation, migration, and invasion, while promoting apoptosis, by targeting and suppressing FoxM1. Our findings suggest that miR-34a-5p is a potential tumor suppressor with therapeutic value. The establishment of the miR-34a-5p/FoxM1 regulatory axis provides new insights into the molecular mechanisms of osteosarcoma. Targeting this axis could offer a promising strategy for improving the prognosis of osteosarcoma.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"90"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAP1-mediated ubiquitination inhibition and CAS6/AXL signaling activation in bladder cancer progression.","authors":"Liang Chen, Zhenjun Liu","doi":"10.1007/s10616-025-00757-z","DOIUrl":"https://doi.org/10.1007/s10616-025-00757-z","url":null,"abstract":"<p><p>This study investigates the role of BRCA1-associated protein 1 (BAP1) in regulating the ubiquitination of SP1, YAP, and PD-L1, as well as its impact on the CAS6/AXL signaling pathway in bladder cancer progression. Transcriptomic analysis was performed using the GSE3167 dataset to identify key gene expression patterns and regulatory mechanisms. A bladder cancer mouse model was established with control (NC), OE-BAP1, and KD-BAP1 groups to assess the effects of BAP1 overexpression and knockdown. Western blot analysis evaluated the expression levels of BAP1, SP1, YAP, PD-L1, CAS6, AXL, and related signaling proteins. Functional assays, including scratch, Transwell, and colony formation, were conducted to assess cell migratory, invasive, and proliferative capacities. Additional groups included BAP1, SP1 inhibitor, BAP1 + SP1 inhibitor, SP1 + anti-PD-L1 monoclonal antibody, and BAP1 + SP1 + anti-PD-L1 combination to evaluate the interplay of these regulatory mechanisms. BAP1 overexpression significantly increased the expression of SP1, YAP, PD-L1, CAS6, AXL, and downstream signaling proteins (PI3K, STAT3, ERK½, MMP-2, and MMP-9), while BAP1 knockdown reduced their levels. Functional assays showed that the BAP1 group exhibited significantly enhanced migratory, invasive, and proliferative abilities compared to controls. Inhibiting SP1 or combining SP1 inhibition with anti-PD-L1 treatment effectively reduced migration, invasion, and proliferation, particularly after 48 h. BAP1 promotes bladder cancer progression by inhibiting the ubiquitination of SP1, YAP, and PD-L1 and activating the CAS6/AXL signaling pathway. These findings highlight BAP1 as a potential therapeutic target for bladder cancer treatment.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"95"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12050257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA SNHG25 facilitates colorectal cancer progression by upregulating PPP2R2D expression through sponging miR-329-3p.","authors":"Yuanqiang Li, Weipeng Liu, Chao Liu, Guangsheng Wang, Xin Zhou","doi":"10.1007/s10616-025-00753-3","DOIUrl":"https://doi.org/10.1007/s10616-025-00753-3","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) have been evidenced to function as pivotal modulators in tumorigenesis. LncRNA SNHG25 is highly expressed in colorectal cancer (CRC), but its specific function in CRC has not been elucidated yet. The expression of SNHG25, miR-329-3p, and PPP2R2D was determined using qRT-PCR analysis and western blot analysis. The influence of the SNHG25/miR-329-3p/PPP2R2D axis on CRC progression was explored through in vitro assays including CCK-8, colony formation, wound healing, Transwell assays and in vivo orthotopic xenografts assay. The interaction between miR-329-3p and SNHG25 or PPP2R2D was examined by RNA pull-down, RIP, and luciferase reporter assays. SNHG25 presented high expression in CRC cell lines. Silencing of SNHG25 suppressed the malignant phenotypes of CRC cells in vitro and tumor growth in vivo. MiR-329-3p, which displayed low expression in CRC cells, was sponged by SNHG25. Downregulation of miR-329-3p reversed the inhibitory effects of SNHG25 silencing on CRC cell malignant behaviors. Additionally, PPP2R2D served as a miR-329-3p downstream target, whose expression was downregulated by overexpressing miR-329-3p. Importantly, overexpression of PPP2R2D rescued SNHG25 silencing-induced repression on CRC cell malignancy. SNHG25 plays a carcinogenic role in CRC via regulation of the miR-329-3p/PPP2R2D axis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00753-3.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"89"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer efficiency of <i>Campylobacter jejuni</i> secretome loaded chitosan nanoparticles on colorectal cancer signaling pathways.","authors":"Ghazale Khodadadi, Masoumeh Saberpour, Bita Bakhshi, Sara Minaeian","doi":"10.1007/s10616-025-00756-0","DOIUrl":"https://doi.org/10.1007/s10616-025-00756-0","url":null,"abstract":"<p><p>Colorectal cancer (CRC) remains as a major health problem with high lethality rate in the world. The innovate therapeutic strategies are essential in CRC management. The purpose of this research was to evaluate the effect of chitosan nanoparticle containing <i>Campylobacter jejuni</i> culture supernatant (CNP/Cj-sup) on genes involved in CRC signaling pathways. CNP/Cj-sup was fabricated via ionotropic gelation method. Dynamic light scattering and transmission electron microscopy (TEM) techniques were employed to characterize of the CNP/Cj-sup including the electrical charge, size distribution, and morphological properties. The loaded protein, released protein, and entrapment efficacy (EE) were assayed utilizing a BCA assay kit. After the evaluation of the viability of Caco-2 (colon adenocarcinoma) and HDF (human dermal fibroblasts) cells against CNP/Cj-sup by MTT assay, subsequently anti-tumor effect of CNP/Cj-sup on genes associated with CRC signaling pathways was assessed via real-time PCR method. The size dispersion of CNP/Cj-sup was 400.6 ± 24.4 nm with an electrical charge of + 4.5 mV. The loaded protein was calculated 1100 µg. The release rate of protein from CNP/Cj-sup was 78% at pH of 6.8 after 48 h, with EE of 74.62%. The viability of Caco-2 and HDF cells against CNP/Cj-sup (1100 µg + 0.05%) was measured 75.8 and 96.5%, respectively after 48 h. CNP/Cj-sup exhibited the highest efficacy in inhibiting the expression of oncogenes <i>TGF-α, Bcl2, TLR4, CEA, TGF-β</i>, and <i>PI3K</i> by to 0.06, 0.34, 0.14, 0.13, 0.08, and 0.14-fold (<i>p</i> value < .0001). Moreover, it led to a significant increase in the expression of the suppressor genes <i>caspase9</i> and <i>PTEN</i> by to 55.7 and 1.8- fold (<i>p</i> value < .0001). CNP/Cj-sup demonstrated the highest efficiency in suppressing <i>TGF-α</i> and enhancing <i>caspase9</i> compared to CNP and Cj-sup. In conclusion, CNP/Cj-sup as an innovative potential anticancer agent, with the ability to modulate genes involved in CRC progression, represents a promising approach to CRC treatment.</p><p><strong>Graphical abstract: </strong>The effect of CNP/Cj-sup on different colorectal cancer signaling pathways.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"93"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and clinical significance of IGF-1 and PDGF in aqueous humor and serum of diabetic patients with visually significant cataract.","authors":"Minghui Chen, Xiuwen Dai, Jing Liang","doi":"10.1007/s10616-025-00754-2","DOIUrl":"https://doi.org/10.1007/s10616-025-00754-2","url":null,"abstract":"<p><p>This study aimed to analyze the expression and clinical significance of IGF-1 and PDGF in the aqueous humor and serum of diabetic patients with visually significant cataract. A retrospective study was conducted on 136 diabetic patients with visually significant cataract who underwent phacoemulsification. Patients were divided into non-complication (n = 82) and complication groups (n = 54) based on postoperative outcomes. Clinical baseline data, as well as IGF-1 and PDGF levels in aqueous humor and serum, were compared between groups. ROC curve analysis was utilized to evaluate the predictive value of IGF-1 and PDGF for postoperative complications. Logistic regression was employed to identify risk factors, and Kaplan-Meier analysis was adopted to assess the impact of IGF-1 and PDGF levels on postoperative complications. No significant differences were found between the two groups in terms of gender, age, BMI, intraocular pressure, surgical eye, phacoemulsification time, phacoemulsification energy, axial length, smoking, drinking history, hypertension, hyperlipidemia, FINS, HbA1C, CRP, or FPG. However, significant differences were observed in disease duration, presence of retinopathy, and IL-6 levels. Patients with complications had significantly higher IGF-1 and PDGF levels in both aqueous humor and serum compared to those without complications. Elevated IGF-1 and PDGF levels were independent risk factors for complications and increased the risk of postoperative complications. Elevated IGF-1 and PDGF levels in aqueous humor and serum are significant independent risk factors for postoperative complications in diabetic patients with visually significant cataract. Both markers can assist in predicting adverse outcomes and are associated with an increased risk of complications.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"94"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Proanthocyanidin B2 alleviates <i>Pg</i>.LPS-induced RAW264.7 cellular inflammation and oxidative stress via PI3K/Akt/NFkB pathway.","authors":"Xin Chen, Zhichun Fang, Junwei Zhao, Xiaoyan Ou","doi":"10.1007/s10616-025-00751-5","DOIUrl":"https://doi.org/10.1007/s10616-025-00751-5","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1007/s10616-025-00734-6.].</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"91"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Protective effects of liquiritin on polycystic ovary syndrome through modulating ovarian granulosa cell proliferation and apoptosis via miR-206/PI3K/AKT pathway.","authors":"Xuan Cui, Shisan Zhou, Yongtao Lin","doi":"10.1007/s10616-025-00746-2","DOIUrl":"https://doi.org/10.1007/s10616-025-00746-2","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1007/s10616-022-00531-5.].</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"97"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of <i>Epimedium</i> in treating diabetic nephropathy based on network pharmacology and experimental validation study.","authors":"Leyu Huang, Hui Li, Ying Han","doi":"10.1007/s10616-025-00748-0","DOIUrl":"10.1007/s10616-025-00748-0","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is a severe complication of diabetes, characterized by chronic inflammation, metabolic disturbances, and progressive renal damage. Natural perennial herb, such as <i>Epimedium</i>, has shown potential therapeutic effects on DN, but its underlying mechanisms remain unclear. This study aimed to explore the pharmacological mechanisms of <i>Epimedium</i> in the treatment of DN through network pharmacology, molecular docking, and experimental validation. Active components of <i>Epimedium</i> were identified using TCMSP and SwissTargetPrediction databases, while DN-related targets were retrieved from GeneCards, DisGeNET, OMIM, and TTD databases. Overlapping targets were analyzed via PPI network and Cytoscape's cytoHubba plugin to identify hub genes. GO and KEGG enrichment analyses were conducted to explore functional pathways. Molecular docking validated the binding affinity between key targets and active components. Finally, high-glucose-induced HK-2 cell injury models were used to verify the protective effects of <i>Epimedium</i> through RT-qPCR, western blotting, and mitochondrial function assays. A total of 224 overlapping targets were identified, with <i>AKT1, TNF, HSP90AA1</i>, and <i>SRC</i> serving as key hub genes. GO and KEGG analyses revealed significant enrichment in pathways such as the PI3K-Akt signaling pathway and lipid metabolism. Molecular docking demonstrated strong interactions between <i>Epimedium</i> components and hub targets. Experimental validation showed that <i>Epimedium</i> restored nephrin and WT1 protein levels, mitigated mitochondrial dysfunction, and reversed high-glucose-induced overexpression of key targets. <i>Epimedium</i> exerts therapeutic effects on DN through multi-target interactions, primarily via the PI3K-Akt pathway, highlighting its potential as a novel treatment for DN.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00748-0.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"82"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cartilage repair: unleashing PRP's potential in organoid models.","authors":"Mahsa Golshan, Hengameh Dortaj, Zeinab Omidi, Mehdi Golshan, Majid Pourentezari, Mehrdad Rajabi, Ali Rajabi","doi":"10.1007/s10616-025-00739-1","DOIUrl":"10.1007/s10616-025-00739-1","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) has emerged as a promising biological therapy in regenerative medicine due to its high concentration of growth factors and cytokines, which promote tissue healing and regeneration. In recent years, its application in cartilage tissue engineering has garnered significant attention. This study explores the synergistic interaction between PRP and cartilage organoids, a novel three-dimensional in vitro culture system that closely mimics the structural and functional properties of native cartilage. Cartilage organoids serve as a physiologically relevant model for studying cartilage development, disease progression, and regeneration. By integrating PRP with cartilage organoids, this review aims to enhance chondrogenesis, extracellular matrix synthesis, and cellular proliferation within the organoids. Emerging evidence suggests that PRP supplementation significantly improves chondrocyte viability, growth, and differentiation in cartilage organoids, thereby accelerating their maturation. This combination holds great potential for advancing cartilage repair strategies, providing a robust platform for preclinical studies, and paving the way for innovative therapeutic approaches for cartilage-related injuries and degenerative diseases. These key aspects-chondrogenesis, matrix synthesis, and cellular proliferation-were specifically selected due to their fundamental roles in cartilage tissue engineering and regeneration. Chondrogenesis is crucial for chondrocyte differentiation and maintenance, matrix synthesis ensures the structural integrity and functional properties of regenerated cartilage, and cellular proliferation supports tissue viability and repair. Addressing these factors is essential, as current cartilage regeneration strategies often suffer from limited long-term efficacy and inadequate extracellular matrix production. By elucidating the synergistic effects of PRP and cartilage organoids in these areas, this study seeks to bridge existing knowledge gaps and provide valuable insights for improving regenerative approaches in clinical applications, particularly for osteoarthritis and cartilage defects.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"86"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different cell culture media on the production and glycosylation of a monoclonal antibody from a CHO cell line.","authors":"Jaeweon Lee, Uriel Ortega-Rodriguez, Chikkathur N Madhavarao, Tongzhong Ju, Thomas O'Connor, Muhammad Ashraf, Seongkyu Yoon","doi":"10.1007/s10616-025-00733-7","DOIUrl":"10.1007/s10616-025-00733-7","url":null,"abstract":"<p><p>Recombinant monoclonal antibodies (mAbs) are commonly produced using Chinese hamster ovary (CHO) cells and the cell culture medium used in bioreactors influences the yield and quality attributes of the protein drug products. The COVID 19 pandemic revealed a vulnerability in the supply chain for necessary reagents (such as culture medium and raw material) for maintaining un-interrupted production of protein drugs with consistent quality. The supply interruption for the cell culture medium ActiPro™ optimized for producing VRC01, an IgG1-κ mAb, from a CHO-K1 cell line, necessitated the search for alternate media. VRC01 mAb is highly glycosylated and can broadly neutralize several strains of Human Immunodeficiency Virus (HIV). We investigated to see if an alternate medium can be used in the production without impacting quality attributes like glycosylation. In our strategy, we used 3 different commercially available media, performed two sets of experiments-with and without media supplements, Cell boost 7a and Cell boost 7b. Cell growth, volumetric production of the mAb protein and glycosylation pattern were compared to identify an alternative medium. Among the tested media based on cell growth, mAb production potential and glycosylation analysis, ActiCHO™ P was found to be a better alternate medium to ActiPro™ medium than EX-CELL® 325 PF CHO medium to produce VRC01 mAb. Overall, the approach used here to establish the impact of variation in medium on protein therapeutic attributes may be used during product development to build in supply chain resilience in drug manufacturing.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s10616-025-00733-7.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 3","pages":"81"},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}