Peiqing Yang, Yong Tan, Shunmin Hu, Zhongzhen Xu, Gan Ji, Shaoli Tai, Xia Zhang, Weizhou Yu
{"title":"柴胡治疗慢性萎缩性胃炎作用机制的网络药理学研究。","authors":"Peiqing Yang, Yong Tan, Shunmin Hu, Zhongzhen Xu, Gan Ji, Shaoli Tai, Xia Zhang, Weizhou Yu","doi":"10.1007/s10616-025-00802-x","DOIUrl":null,"url":null,"abstract":"<p><p>Radix Bupleuri (RB) is used as a traditional Chinese medicine for treating gastric disorders. However, the active components and pharmacological targets of RB are unknown. In this study, we aimed to reveal the pharmacological effects of RB on gastritis by combining a rat model and a network pharmacology approach. The results showed that quercetin was the most activate ingredient in the treatment of chronic atrophic gastritis (CAG). The molecular docking results showed that RB had a strong affinity with TNF. In vivo and in vitro experiments showed that RB alleviated MNNG-induced gastritis by inhibiting the TNF-α/NF-κB signaling pathway. Through network pharmacology, molecular docking, and in vitro and in vivo experiments, the mechanisms and potential effects of RB on CAG were demonstrated, and a theoretical basis for RB in the treatment of CAG was provided.</p>","PeriodicalId":10890,"journal":{"name":"Cytotechnology","volume":"77 4","pages":"136"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226447/pdf/","citationCount":"0","resultStr":"{\"title\":\"A web-based pharmacological approach to the mechanism of action of Radix Bupleuri in the treatment of chronic atrophic gastritis.\",\"authors\":\"Peiqing Yang, Yong Tan, Shunmin Hu, Zhongzhen Xu, Gan Ji, Shaoli Tai, Xia Zhang, Weizhou Yu\",\"doi\":\"10.1007/s10616-025-00802-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Radix Bupleuri (RB) is used as a traditional Chinese medicine for treating gastric disorders. However, the active components and pharmacological targets of RB are unknown. In this study, we aimed to reveal the pharmacological effects of RB on gastritis by combining a rat model and a network pharmacology approach. The results showed that quercetin was the most activate ingredient in the treatment of chronic atrophic gastritis (CAG). The molecular docking results showed that RB had a strong affinity with TNF. In vivo and in vitro experiments showed that RB alleviated MNNG-induced gastritis by inhibiting the TNF-α/NF-κB signaling pathway. Through network pharmacology, molecular docking, and in vitro and in vivo experiments, the mechanisms and potential effects of RB on CAG were demonstrated, and a theoretical basis for RB in the treatment of CAG was provided.</p>\",\"PeriodicalId\":10890,\"journal\":{\"name\":\"Cytotechnology\",\"volume\":\"77 4\",\"pages\":\"136\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12226447/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cytotechnology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10616-025-00802-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytotechnology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10616-025-00802-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/3 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
A web-based pharmacological approach to the mechanism of action of Radix Bupleuri in the treatment of chronic atrophic gastritis.
Radix Bupleuri (RB) is used as a traditional Chinese medicine for treating gastric disorders. However, the active components and pharmacological targets of RB are unknown. In this study, we aimed to reveal the pharmacological effects of RB on gastritis by combining a rat model and a network pharmacology approach. The results showed that quercetin was the most activate ingredient in the treatment of chronic atrophic gastritis (CAG). The molecular docking results showed that RB had a strong affinity with TNF. In vivo and in vitro experiments showed that RB alleviated MNNG-induced gastritis by inhibiting the TNF-α/NF-κB signaling pathway. Through network pharmacology, molecular docking, and in vitro and in vivo experiments, the mechanisms and potential effects of RB on CAG were demonstrated, and a theoretical basis for RB in the treatment of CAG was provided.
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The scope of the Journal includes:
1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products.
2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools.
3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research.
4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy.
5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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