Crop Science最新文献_第5页

Exploring differentially expressed genes and associated biological processes across embryos with different seed dormancy in barley (Hordeum vulgare)

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-07 DOI: 10.1002/csc2.70000

Yuta Aoyagi Blue, Hideki Hirakawa, Youko Oono, Shingo Nakamura

{"title":"Exploring differentially expressed genes and associated biological processes across embryos with different seed dormancy in barley (Hordeum vulgare)","authors":"Yuta Aoyagi Blue, Hideki Hirakawa, Youko Oono, Shingo Nakamura","doi":"10.1002/csc2.70000","DOIUrl":"https://doi.org/10.1002/csc2.70000","url":null,"abstract":"Barley (Hordeum vulgare) has a wide range of seed dormancy. Seed dormancy is a crucial trait for plant survival and crop production. To explore genes and functions associated with the difference in barley seed dormancy, we performed differentially expressed gene and gene ontology (GO) enrichment analyses on seed embryos from a wild barley accession H602 with strong dormancy, a cultivated malting barley accession Kanto Nakate Gold (KNG) with weak dormancy, and reciprocal crosses between the two H602 × KNG and KNG × H602. RNA was extracted from embryos in seeds harvested at physiological maturity and dried for 1 week. We also assessed expression levels of known quantitative trait locus genes linked to seed dormancy. In H602 and H602 × KNG, 417 genes showed significantly higher expression than KNG and KNG × H602, with 14 GO terms enriched, including stress response and sugar metabolism. Genes involved in DNA repair were also enriched for highly expressed genes in H602 than KNG. In KNG and KNG × H602, 198 genes showed significantly higher expression than H602 and H602 × KNG, with 60 GO terms enriched, including development and auxin transport. Genes involved in translation were also enriched for highly expressed genes in KNG × H602 than H602 × KNG. Our findings indicated that genes involved in abiotic stress response, sugar metabolism, and DNA repair would be associated with strong seed dormancy, whereas genes involved in development and translation would be associated with weak dormancy in barley.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70000","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of Ehd1 prolongs basic vegetative growth periods to boost biomass and grain yield in rice

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-06 DOI: 10.1002/csc2.70009

Limin Wang, Jiahui Zhu, Jiaoyan Liu, Jiatao Zhan, Yuechen Li, Ru Wang, Huiwu Tang

{"title":"Mutation of Ehd1 prolongs basic vegetative growth periods to boost biomass and grain yield in rice","authors":"Limin Wang, Jiahui Zhu, Jiaoyan Liu, Jiatao Zhan, Yuechen Li, Ru Wang, Huiwu Tang","doi":"10.1002/csc2.70009","DOIUrl":"10.1002/csc2.70009","url":null,"abstract":"Heading date is a pivotal agronomic trait that influences grain yield, and it is determined by florigen genes. However, the regulation of heading date and its impact on other agronomic traits remain to be fully understood. In this study, we identified a mutant named late-flowering and big panicle 1 (lbp1) in rice; we surveyed the growth and yield traits in lbp1. Changes in gene expression between lbp1 and wild type (WT) were determined by RNA-seq. lbp1 exhibits delayed heading of 1 month and enhancements in various agronomic traits. Compared with WT plants, lbp1 showed 336.9% higher biomass production, 27.7% longer panicle length, 41.2% higher grain numbers, 29.4% and 56.6% more primary and secondary branch numbers, and an overall 58.7% higher grain yield. Map-based cloning pinpointed that the mutant phenotypes stemmed from a mutation in the early heading date 1 (Ehd1) gene. Through RNA-seq and quantitative reverse transcription-polymerase chain reaction analysis, we observed significantly lower expression levels of heading date 3a (Hd3a) and RICE FLOWERING LOCUS T 1 (RFT1) in lbp1 compared to WT plants. Additionally, the expression levels of several MADS-box genes were downregulated in the mutant. Our findings indicate that negative regulation of the Ehd1-Hd3a/RFT1 pathway leads to delayed heading, enhanced biomass production, and increased grain yield. This discovery presents an effective breeding strategy for developing new japonica rice varieties with prolonged vegetative growth periods suitable for cultivation in southern China and other low-latitude regions.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosynthesis-driven yield gains in global wheat breeding trials

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-06 DOI: 10.1002/csc2.70005

Qiansu Ding, Xiaoxing Zhen, Walid Sadok

{"title":"Photosynthesis-driven yield gains in global wheat breeding trials","authors":"Qiansu Ding, Xiaoxing Zhen, Walid Sadok","doi":"10.1002/csc2.70005","DOIUrl":"10.1002/csc2.70005","url":null,"abstract":"Increasing wheat yields is critical to meet the demands of a growing population and ensure socioeconomic stability. Rising photosynthesis is being proposed as a promising avenue to increase wheat yields, but evidence for this is conflicting across studies. To test the viability of this hypothesis, here we report a systematic and quantitative literature review examining the association between historic yield increases due to breeding that occurred over 129 years (1890–2019) across the globe and light-saturated photosynthesis (Asat) for 273 wheat commercial varieties released to farmers in five continents. A positive and highly significant relationship between trial-corrected grain yield and cultivars’ year of release (YOR) was found globally (R2 = 0.56), with an annual rate of yield increase of 0.84%. Trial-corrected seed-fill Asat increased globally at a pace of 0.58% year−1, and this association was the strongest among the examined four phenological stages. Detrended (from YOR), trial-corrected grain yield and Asat were found to be correlated, with this association being significant only during seed-fill. The analysis also suggests that historical yield gains may also have been achieved by an increase in water uptake during seed-fill, but it points to the need for more standardized protocols for measuring gas exchange in such vintage/era studies. Overall, these insights indicate that breeding for higher wheat yields was accompanied by physiological alterations to improve Asat and, possibly, increase water use during the critical seed-fill period, two traits that are also likely to enhance wheat climate resilience.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved cis-acting motifs and localization of MFT2 transcripts and MFT2 protein in barley and rice 大麦和水稻中保守的顺式作用基序以及 MFT2 转录本和 MFT2 蛋白的定位

IF 2 3区农林科学

Crop Science Pub Date : 2025-02-06 DOI: 10.1002/csc2.70007

Shigeko Utsugi, Hiroyuki Kawahigashi, Akemi Tagiri, Rie Kikuchi, Kohei Mishina, Hiromi Morishige, Shingo Nakamura

{"title":"Conserved cis-acting motifs and localization of MFT2 transcripts and MFT2 protein in barley and rice","authors":"Shigeko Utsugi, Hiroyuki Kawahigashi, Akemi Tagiri, Rie Kikuchi, Kohei Mishina, Hiromi Morishige, Shingo Nakamura","doi":"10.1002/csc2.70007","DOIUrl":"10.1002/csc2.70007","url":null,"abstract":"Pre-harvest sprouting, in which grain germinates while still on the spikes, causes substantial yield and quality losses, particularly in regions where the rainy season and harvest times overlap. Breeders have used a natural mutation in the A-box motif in the promoter of wheat (Triticum aestivum) MFT2 gene on chromosome 3A (TaMFT-3A) to prevent pre-harvest sprouting in wheat cultivars in East Asia. Here, we examined the barley (Hordeum vulgare) and rice (Oryza sativa) orthologs of TaMFT-3A. RNA in situ hybridization showed that the rice and barley orthologs are primarily expressed in the scutellum, indicating that these three MFT2 genes have a common expression pattern during seed development. Analysis of the cis-acting regulatory elements of their promoter sequences showed that the promoters share eight seed-specific cis-acting RY motifs and a binding site for B3-domain transcription factors, the main regulators of seed development. Moreover, we found tandemly repeated A-box motifs in the promoters of TaMFT on chromosomes 3B and 3D, but a single motif on chromosome 3A, possibly explaining why the A-box motif mutation in TaMFT-3A increases dormancy. Our immunohistochemical observations of green fluorescent protein (GFP)-tagged MFT2 in transgenic rice plants detected rice MFT2 (OsMFT2)-GFP in the scutellum and the scutellar epithelium, which is an important tissue for initiating germination. These findings improve our understanding of the role of MFT2, providing information for reducing pre-harvest sprouting in cereals.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hairy vetch (Vicia villosa Roth) germplasm contains a cryptic second species (Vicia varia Host)

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-31 DOI: 10.1002/csc2.21455

Neal Tilhou, Lisa Kissing Kucek, Virginia Moore, Solveig Hanson, Chris Reberg-Horton, Matthew R. Ryan, Nancy Jo Ehlke, Amy Bartow, Brandon Carr, Joel Douglas, John Englert, John Raasch, Alyssa J. Woodard, Jamie Crawford, Ryan Crawford, Shahjahan Ali, Suresh Bhamidimarri, Steven Mirsky, Maria J. Monteros, Gerry Moore, Audrey V. Gamble, Nithya Rajan, Sruthi Narayanan, Erin R. Haramoto, Nicholas T. Basinger, Virginia R. Sykes, Amanda McWhirt, Mark S. Reiter, Heathcliffe Riday

{"title":"Hairy vetch (Vicia villosa Roth) germplasm contains a cryptic second species (Vicia varia Host)","authors":"Neal Tilhou, Lisa Kissing Kucek, Virginia Moore, Solveig Hanson, Chris Reberg-Horton, Matthew R. Ryan, Nancy Jo Ehlke, Amy Bartow, Brandon Carr, Joel Douglas, John Englert, John Raasch, Alyssa J. Woodard, Jamie Crawford, Ryan Crawford, Shahjahan Ali, Suresh Bhamidimarri, Steven Mirsky, Maria J. Monteros, Gerry Moore, Audrey V. Gamble, Nithya Rajan, Sruthi Narayanan, Erin R. Haramoto, Nicholas T. Basinger, Virginia R. Sykes, Amanda McWhirt, Mark S. Reiter, Heathcliffe Riday","doi":"10.1002/csc2.21455","DOIUrl":"10.1002/csc2.21455","url":null,"abstract":"Hairy vetch is a promising legume cover crop (Vicia villosa Roth) for the northern United States. Based on evidence from molecular markers, multi-site evaluations, and morphological observations, a distinct second species exists within US hairy vetch germplasm, referred to hereafter as smooth vetch (Vicia varia Host). Morphologically, hairy vetch is highly variable, but this study found statistically significant differences between smooth and hairy vetch in visual pubescence scores, plant maturity, and calyx lobe lengths. We used a panel of single sequence repeat (SSR) markers to assign cultivars and breeding materials to the two species and found that many commercial cultivars are smooth vetch. Interestingly, the SSR panel indicated that woollypod vetch (Vicia dasycarpa Ten.) is a subpopulation of hairy vetch. Based on an elastic net model trained with multi-site trial results from >35 site-years, smooth vetch is not winter hardy in the northern United States but has superior performance relative to hairy vetch in the south-central and Pacific Northwest United States. Specifically, smooth vetch has greater performance in environments with mild winters, cool spring temperatures, or low clay soils. Because of these differences in adaptation, differentiating these species will greatly improve agronomic outcomes and accelerate ongoing cover crop breeding progress.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Higher seed yield through selection for reduced seed shattering in Italian ryegrass

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-30 DOI: 10.1002/csc2.70002

Jenny Kiesbauer, Roland Kölliker, Maria Hug, Meril Sindelar, Linda Helene Schlatter, Jonathan Ohnmacht, Bruno Studer, Christoph Grieder

{"title":"Higher seed yield through selection for reduced seed shattering in Italian ryegrass","authors":"Jenny Kiesbauer, Roland Kölliker, Maria Hug, Meril Sindelar, Linda Helene Schlatter, Jonathan Ohnmacht, Bruno Studer, Christoph Grieder","doi":"10.1002/csc2.70002","DOIUrl":"10.1002/csc2.70002","url":null,"abstract":"Seed shattering, that is, the loss of seeds at ripening stage shortly before or during seed harvest, is strongly reducing seed yield in Italian ryegrass (Lolium multiflorum Lam.). The aim of this study was to evaluate the possibility to reduce seed shattering within breeding germplasm via recurrent phenotypic selection on spaced plants. Starting from a founder population of 300 plants not previously selected for seed shattering, two cycles of phenotypic selection for high and low seed shattering were performed based on spaced plants. The resulting five populations with different levels of selection for seed shattering (selected once or twice for low seed shattering, selected once or twice for high seed shattering, and no [random] selection) were phenotyped in plot trials with two harvesting dates (early and late). Seed shattering was highest in the population selected twice for increased seed shattering (7.92% for early and 28.44% for late harvesting), followed by the population selected once for increased seed shattering (7.34% early, 21.56% late), the non-selected population (6.12% early, 20.22% late), the population selected once for decreased seed shattering (4.13% early, 16.73% late), and the population selected twice for decreased seed shattering (2.41% early, 9.31% late). Selecting twice for low seed shattering resulted in a reduction of seed shattering by 10.91 percentage points at late harvest when compared to the non-selected population. Thus, recurrent phenotypic selection on spaced plants is appropriate to efficiently reduce seed shattering and to increase seed yield in sown plots, which is highly relevant for seed production.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting inbred parent synchrony at flowering for maize hybrid seed production by integrating crop growth model with whole genome prediction

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-30 DOI: 10.1002/csc2.21453

Anabelle Laurent, Eugenia Munaro, Honghua Zhao, Frank Technow, Eric Whitted, Randy Clark, Juan Pablo San Martin, Radu Totir

{"title":"Predicting inbred parent synchrony at flowering for maize hybrid seed production by integrating crop growth model with whole genome prediction","authors":"Anabelle Laurent, Eugenia Munaro, Honghua Zhao, Frank Technow, Eric Whitted, Randy Clark, Juan Pablo San Martin, Radu Totir","doi":"10.1002/csc2.21453","DOIUrl":"10.1002/csc2.21453","url":null,"abstract":"One of the challenges of maize (Zea mays) hybrid seed production is to ensure synchrony at flowering of the two inbred parents of a hybrid, which depends on the specific parental combination and environmental conditions of the production field. Maize flowering can be simulated using a mechanistic crop growth model that converts thermal time accumulation to leaf numbers based on inbred-specific physiological parameter values. Heretofore, these inbred-specific physiological parameters need to be measured or assigned based on prior knowledge. Here, we leverage genetic, environmental, and management data to predict physiological parameters and simulate flowering phenotypes by using whole genome prediction methodology combined with a crop growth model (CGM–WGP) as part of in-field in-season inbred growth development. We use two estimation sets that differ in terms of management and weather information to test the robustness of our approach. As part of our findings, we demonstrate the importance of defining informative priors to generate biologically meaningful predictions of unobserved physiological parameters. Our CGM–WGP infrastructure is efficient at simulating flowering phenotypes. An important practical application of our method is the ability to recommend differential planting intervals for male and female maize inbreds used in commercial seed production fields to synchronize male and female flowering.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating UAV-based multispectral remote sensing and machine learning for detection and classification of chocolate spot disease in faba bean

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-30 DOI: 10.1002/csc2.21454

Shirin Mohammadi, Anne Kjersti Uhlen, Heidi Udnes Aamot, Jon Arne Dieseth, Sahameh Shafiee

{"title":"Integrating UAV-based multispectral remote sensing and machine learning for detection and classification of chocolate spot disease in faba bean","authors":"Shirin Mohammadi, Anne Kjersti Uhlen, Heidi Udnes Aamot, Jon Arne Dieseth, Sahameh Shafiee","doi":"10.1002/csc2.21454","DOIUrl":"10.1002/csc2.21454","url":null,"abstract":"Chocolate spot (CS), caused by Botrytis fabae, is one of the most destructive fungal diseases affecting faba bean (Vicia faba L.) globally. This study evaluated 33 faba bean cultivars across two locations and over 2 years to assess genetic resistance and the effect of fungicide application on CS progression. The utility of unmanned aerial vehicle–mounted multispectral camera for disease monitoring was examined. Significant variability was observed in cultivar susceptibility, with Bolivia exhibiting the highest level of resistance and Louhi, Sampo, Vire, Merlin, Mistral, and GL Sunrise proving highly susceptible. Fungicide application significantly reduced CS severity and improved yield. Analysis of canopy spectral signatures revealed the near-infrared and red edge bands, along with enhanced vegetation index (EVI) and soil adjusted vegetation index, as most sensitive to CS infection, and they had a strong negative correlation with CS severity ranging from −0.51 to −0.71. In addition, EVI enabled early disease detection in the field. Support vector machine accurately classified CS severity into four classes (resistant, moderately resistant, moderately susceptible, and susceptible) based on spectral data with higher accuracy after the onset of disease compared to later in the season (accuracy 0.75–0.90). This research underscores the value of integrating resistant germplasm, sound agronomic practices, and spectral monitoring for effectively identification and managing CS disease in faba bean.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic analysis of forage, seed, and turf quality in tall fescue: Unraveling inheritance patterns and interrelationships

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-26 DOI: 10.1002/csc2.21447

Fatemeh Pirnajmedin, Mohammad Mahdi Majidi, Maryam Safari

{"title":"Genetic analysis of forage, seed, and turf quality in tall fescue: Unraveling inheritance patterns and interrelationships","authors":"Fatemeh Pirnajmedin, Mohammad Mahdi Majidi, Maryam Safari","doi":"10.1002/csc2.21447","DOIUrl":"10.1002/csc2.21447","url":null,"abstract":"Tall fescue (Festuca arundinacea Schreb. syn. Lolium arundinaceum), an important cool-season grass, has limited understanding regarding its genetic inheritance patterns and the potential for simultaneous selection across forage, seed, and turf traits. In this study, 24 half-sib families derived from polycrosses, along with their corresponding parental genotypes, were assessed for different agro-morphological, seed, and turf quality characteristics in the field for 2 years (2019–2020). High genotypic variation was observed for all the measured traits. Moderate narrow-sense heritability (h2PFM) for turf quality and seed-related traits indicated that genetic variance predominates in total phenotypic variance of these traits. Low value of h2PFM (0.25) for dry forage yield (DFY) shows the high environmental influence on the expression of this economic trait. Indirect selection to improve DFY was more effective through its components, such as crown diameter (h2PFM = 0.43) and plant height (h2PFM = 0.48), which had higher heritability and positive correlation with forage yield. However, for seed and turf quality traits, direct selection would be possible during recurrent selection programs. The simultaneous selection for both forage yield and seed yield would be possible due to the positive correlation between them. Based on the application of multivariate analysis, parental genotypes and half-sib families with possible utility as forage and seed use or turf application were recognized, which can be used in the future breeding programs for developing synthetic varieties.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143044205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bambara groundnut [Vigna subterranea (L.) Verdc.] genetic diversity and population structure assessed through next-generation sequencing technologies: Restriction-site-associated DNA sequencing 班巴拉花生[地下葡萄属（L.）]Verdc。通过下一代测序技术评估遗传多样性和群体结构：限制性位点相关DNA测序

IF 2 3区农林科学

Crop Science Pub Date : 2025-01-20 DOI: 10.1002/csc2.21444

Nsovo H. Baloyi, Alvera A. Voster, Pieter Swanepoel

{"title":"Bambara groundnut [Vigna subterranea (L.) Verdc.] genetic diversity and population structure assessed through next-generation sequencing technologies: Restriction-site-associated DNA sequencing","authors":"Nsovo H. Baloyi, Alvera A. Voster, Pieter Swanepoel","doi":"10.1002/csc2.21444","DOIUrl":"10.1002/csc2.21444","url":null,"abstract":"The importance of underutilized crops in the diversification of diets for both humans and animals, among other uses, has been highlighted in literature in recent times. Underutilized crops are especially important because of their potential to provide nutrient-packed, climate-resilient, and sustainable farming practices. One such crop is Bambara groundnut [Vigna subterranea (L.) Verdc], whose genetic potential has not been afforded sufficient research attention. For most of the rural areas in Sub-Saharan Africa, it is a great source of food and income and is most valued for its nutrient richness and ability to thrive in marginal land. However, farmers grapple with the lack of high agronomic quality seeds where production of the crop is concerned. The aim of this study was to establish an easy basis for selecting seeds that are of favorable agronomic potential by assessing whether a singular characteristic (seed coat color) was sufficient to group landraces. Restriction-site-associated DNA (RAD) sequencing was used for the first time to assess the genetic variations and/or similarities in 48 Bambara groundnut landraces. The findings revealed that there are two populations that are genetically variable among the chosen 48 landraces; however, these variations were not as a result of a singular morphological attribute. Therefore, farmers cannot use coat color alone to select for landraces that are of better agronomic quality.","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.21444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142991497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0