Crop Science最新文献

{"title":"Environmental adaptation of small-seeded lentils (Lens culinaris) in Indian climates: Insights into crop–environment interactions, mega-environments, and breeding approaches","authors":"Ashok Kumar Parihar,&nbsp;Shailesh Tripathi,&nbsp;Kali Krishna Hazra,&nbsp;Amrit Lamichaney,&nbsp;Debjyoti Sen Gupta,&nbsp;Jitendra Kumar,&nbsp;Anil Kumar Singh,&nbsp;Jai Dev Sharma,&nbsp;Parvez Ahmad Sofi,&nbsp;Ajaz Ahmad Lone,&nbsp;Muniyandi Samuel Jeberson,&nbsp;Ashok Kumar,&nbsp;Sarvjeet Singh,&nbsp;Harsh Kumar Dikshit,&nbsp;Murlidhar Aski,&nbsp;Sumer Singh Punia,&nbsp;Manoj Katiyar,&nbsp;Anil Kumar Singh,&nbsp;Subhash Chandra,&nbsp;Chandra Shekhar Mahto,&nbsp;Hironya Kumar Borah,&nbsp;Amitava Banerjee,&nbsp;Arpita Das,&nbsp;Deepak Singh,&nbsp;Sanjeev Gupta,&nbsp;Girish Prasad Dixit","doi":"10.1002/csc2.70090","DOIUrl":"https://doi.org/10.1002/csc2.70090","url":null,"abstract":"<p>This study aimed to evaluate the impact of diverse environments on agronomic traits of small-seeded lentils in India and to identify strategic environment(s) for crop improvement using empirical approaches. Sixteen small-seeded lentil genotypes were tested across 16 locations, covering North Hill Zone (NHZ), North Western Plain Zone (NWPZ), and North Eastern Plain Zone (NEPZ). Crop yield exhibited a quadratic response to maximum temperature during flowering (TMAX_F) and minimum temperature during reproductive period (TMIN_RP), indicating that both higher and lower TMAX_F (optimal = 23.5°C) and TMIN_RP (optimal = 12.0°C) negatively affected yield. Low temperatures extended crop duration, and correlation analysis confirmed that environment-induced variations in vegetative or maturity periods significantly influenced yield, as further validated by regression models and principal component analysis. While an extended reproductive period increased seed weight, its association with grain yield was non-significant. Among the tested zones, NWPZ recorded the highest average yield (1359 kg ha⁻¹), while NEPZ (1269 kg ha⁻¹) demonstrated greater yield consistency across locations. Genotype, environment, and genotype-by-environment interaction (GEI) contributed 6%, 53%, and 36% of the total yield variation, respectively. Biplot analysis identified three distinct mega-environments, with Durgapura (NWPZ), Varanasi (NEPZ), Faizabad (NEPZ), and Berthin (NHZ) emerging as ideal testing sites. This study highlights the significant influence of diverse agroclimatic conditions on crop yield and emphasizes the need for region-specific breeding to harness positive GEI (at mega-environment scale) and to prioritize early flowering and optimal seed traits to achieve broader adaptability across Indian climates.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasticity of maize (Zea mays L.) roots in water-deficient and nitrogen-limited soil","authors":"Yutong Jiang,&nbsp;Joann K. Whalen","doi":"10.1002/csc2.70084","DOIUrl":"https://doi.org/10.1002/csc2.70084","url":null,"abstract":"<p>Maize (<i>Zea mays</i> L.), a versatile global crop with diverse application in food, feed, and industry, experiences production challenges due to water deficits and concurrent nitrogen (N) limitations. Maize relies on multiple root types, including primary, seminal, crown, brace roots, and associated lateral roots, to acquire water and nutrients from the soil. These root types exhibit distinct soil water and N uptake capacities, dynamically adjusting their morphological and anatomical traits to optimize resource uptake. This review summarizes the plastic responses of maize roots to soil water deficit, N limitation, and lack of other essential nutrients. In response to water deficit, maize axial roots bend toward regions with higher water availability and produce more lateral roots. Simultaneously, the diameter of the root xylem decreases, facilitating sustained water uptake from dry soil. In response to N limitation, maize increases lateral root length, reduces xylem area, and increases cortical aerenchyma. This review enhances our understanding of maize root adaptability in water-limited and N-poor soils, offering valuable perspectives on optimizing root growth for improved water and N acquisition.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing genetic variation, genotype-environment interactions, and genomic prediction for cassava garri quality","authors":"Chinedozi Amaefula,&nbsp;Tesfahun Alemu Setotaw,&nbsp;Justice Obinna Okoronkwo,&nbsp;Ugo Chijioke,&nbsp;Lydia Chidinma Ezenwaka,&nbsp;Joseph Onyeka,&nbsp;Ismail Rabbi,&nbsp;Hale Ann Tufan,&nbsp;Chiedozie Ngozi Egesi,&nbsp;Jean-Luc Jannink","doi":"10.1002/csc2.70032","DOIUrl":"10.1002/csc2.70032","url":null,"abstract":"<p>In this study, we assessed the genetic and genotype by environment interaction variation and genomic prediction of the garri quality traits of cassava (<i>Manihot esculenta</i> Crantz). The study was conducted in the National Root Crop Research Institute experimental sites in Umudike and Otobi, Nigeria, in two planting seasons of 2021 and 2022. We estimated the broad and narrow-sense heritability and correlation among measured physicochemical parameters on the garri and dried cassava roots (chips). A fivefold cross-validation scheme was used to calculate the accuracy of the genomic prediction. A significant negative correlation was observed between dry matter content, swelling index, water absorption capacity, and amylose content in garri. The swelling index had a significant negative correlation with swelling power, bulk density, amylose content of cassava chips, crude fiber, and dry matter content. Bulk density had a significant negative correlation with the crude fiber of garri and a positive correlation with the amylose content of cassava chips, the crude fiber of cassava chips, and the dry matter content. Broad-sense heritability ranged between 0 and 0.19 for garri traits and 0.02 and 0.49 for chips. Narrow-sense heritability ranged between 0 and 0.18 for garri quality traits and 0 and 0.20 for chips. A significant genotype × environment effect was observed for most of the evaluated traits of chips and garri quality. The predictability of the sugar content, swelling index, swelling power, and water absorption capacity of garri was higher than that of the amylose content, bulk density, and starch content of garri. There was low predictive accuracy for the quality traits garri studied. The sugar content, swelling power, and water absorption capacity of cassava chips averaged approximately 20%. This study provides valuable insights into assessing the genetic variation, correlation, and genomic selection within breeding programs aimed at enhancing the quality traits of cassava for end users.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into root system architecture of buckwheat through genome-wide association mapping-first study","authors":"Diksha Singh,&nbsp;Jebi Sudan,&nbsp;Anjali Verma,&nbsp;Basharat Bhat,&nbsp;Uneeb Urwat,&nbsp;Asif B Shikari,&nbsp;M. Ashraf Bhat,&nbsp;Parvaze Ahmad Sofi,&nbsp;Sajad Majeed Zargar","doi":"10.1002/csc2.70092","DOIUrl":"https://doi.org/10.1002/csc2.70092","url":null,"abstract":"<p>Buckwheat, a valuable nutraceutical crop, is predominantly cultivated in low-input marginal farming systems and faces productivity challenges due to abiotic stresses, including drought. Roots play a crucial role in stress tolerance and nutrient acquisition. This study aimed to comprehensively investigate the root system architecture (RSA) of 117 buckwheat genotypes, including <i>Fagopyrum esculentum</i> and <i>Fagopyrum tataricum</i>, using phenotypic and genomic analyses. Plants were grown under controlled conditions, and root traits were analyzed using high-resolution scanning and image analysis. Phenotypic analysis revealed significant variability in root traits, with <i>F. tataricum</i> displaying superior RSA attributes compared to <i>F. esculentum</i>. Genome-wide association studies identified 73 significant quantitative trait loci (QTLs) associated with 27 traits, primarily related to RSA. High-density genotyping using the genotyping-by-sequencing approach enabled the identification of single nucleotide polymorphism and candidate genes within a 100-kb region surrounding these QTLs. Correlation analyses highlighted key traits such as root surface area, root volume, and branching frequency, which influence stress tolerance. This pioneering study provides valuable insights into the genetic basis of RSA in buckwheat. Identified QTLs and candidate genes offer potential for marker-assisted breeding to develop drought-resilient, high-yielding buckwheat varieties. These findings contribute to improving buckwheat's productivity and stress adaptation, supporting its role in sustainable agriculture and global food security.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploiting barley landraces to reintroduce lost genetic diversity: Resistance to net form net blotch","authors":"Nola K. D'Souza,&nbsp;Hoan Dinh,&nbsp;Julie A. Lawrence,&nbsp;Elzette Palmiero,&nbsp;Mariano J. Muria-Gonzalez,&nbsp;Sanjiv Gupta,&nbsp;Kerrie Forrest,&nbsp;Simon R. Ellwood","doi":"10.1002/csc2.70088","DOIUrl":"https://doi.org/10.1002/csc2.70088","url":null,"abstract":"<p>Centuries of barley (<i>Hordeum vulgare</i>) domestication and selection has resulted in reduced genetic diversity in modern cultivars, limiting breeder's options to select desirable traits. Barley landraces, heirloom varieties, and wild relatives are substantially more variable and can be exploited to reintroduce favorable genes and alleles. Five doubled haploid populations were phenotyped for net form net blotch (NFNB) disease, caused by the pathogen <i>Pyrenophora teres</i> f. <i>teres</i>, at three growth stages. Major, moderate, and minor effect quantitative trait loci (QTLs) associated with NFNB resistance were detected on six of the seven barley chromosomes, with percentage of explained variance (PEV) ranging from less than 10% to over 70%. Previously established major (PEV &gt; 50%) and moderate (PEV 10%–40%) effect QTLs on 3H and 6H were detected against the Australian isolate used, as well as moderate and minor QTLs (PEV &lt; 10%) distributed on 2H, 3H, 4H, and 5H. Differences in effect sizes of individual QTL were apparent between growth stages, tapering up toward heading or down from seedlings, together with growth stage-specific and synergistic QTL. Several of these QTL represent novel sources of resistance that may be combined for durable NFNB resistance.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure of Colombian sweet potato genotypes reveal possible adaptations to specific environmental conditions","authors":"Jhon A. Berdugo-Cely,&nbsp;Jazmín Vanessa Pérez-Pazos,&nbsp;Amparo Rosero","doi":"10.1002/csc2.70091","DOIUrl":"https://doi.org/10.1002/csc2.70091","url":null,"abstract":"<p><i>Ipomoea batatas</i>, commonly known as sweet potato, is a widely cultivated and consumed crop. It is valued for its storage roots, which serve as an important source of energy and essential nutrients. However, in Colombia, sweet potato remains underutilized, with limited cultivation and consumption. Native and alternative crops are central to government strategies that link biodiversity to food security and sovereignty. This study evaluates the diversity and genetic structure of 438 genotypes from the Colombian Sweet Potato Collection (CSPC), using both morphological and molecular data, and proposes a core collection to support conservation strategies. Additionally, a genetic comparison with the USDA collection was conducted to contextualize the diversity of the CSPC within a global framework. Substantial diversity was observed among Colombian sweet potato genotypes. Four distinct phenotypic groups were identified based on their origin in the Andean and Caribbean regions, exhibiting variations in their ability to develop storage roots and floral structures under the conditions of the evaluation site. Molecular analyses revealed over 37,000 single nucleotide polymorphism (SNP) variants, allowing the identification of six genetic groups associated with geographic origin (Andean and Caribbean regions), altitude, levels of genetic diversity, and cultivation status (landraces, breeding lines, or wilds). A core collection of 100 genotypes was proposed to represent the genetic diversity of the CSPC, incorporating both morphological and molecular data. Finally, the CSPC was compared with the USDA collection, revealing clear genetic differentiation between the two collections and higher levels of genetic diversity in the CSPC. These findings highlight the broad genetic diversity of sweet potato in Colombia, with its genetic structure strongly influenced by local environmental conditions. They also confirm that Colombia is an important center of diversity for this crop. These insights are essential for advancing research, strengthening conservation efforts, and optimizing the use of sweet potato genetic resources in the country.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onerice breeding framework: An end-to-end system to develop better varieties faster","authors":"Sankalp Bhosale,&nbsp;Parthiban Thathapalli Prakash,&nbsp;Suresh Babu Kadaru,&nbsp;Waseem Hussain,&nbsp;Shalabh Dixit,&nbsp;Jauhar Ali,&nbsp;John D. Platten,&nbsp;Md Rafiqul Islam,&nbsp;Vikas Kumar Singh,&nbsp;Rosemary Murori,&nbsp;Alexis Ndayiragije,&nbsp;Ajay Panchbhai,&nbsp;Swati Nayak,&nbsp;Princess Dela Cruz,&nbsp;Matty Demont,&nbsp;Jerome Bartholome,&nbsp;Giovanny Covarrubias-Pazaran,&nbsp;Joshua N. Cobb,&nbsp;Hans Bhardwaj","doi":"10.1002/csc2.70087","DOIUrl":"https://doi.org/10.1002/csc2.70087","url":null,"abstract":"<p>Breeding in the Consultative Group on International Agricultural Research (CGIAR) system is an intricate process that integrates the contributions of market research, pre-breeding, breeding, breeding operations, and seed systems. Therefore, a well-defined framework is critical for the effective and efficient operation of a breeding program. The OneRice Breeding Framework developed at the International Rice Research Institute (IRRI) integrates these components, from initial market research to establish breeding goals, creating breeding strategies for improved product design and development, and swiftly testing and replacing products through effective seed systems. The framework represents a cutting-edge breeding approach that offers comprehensive guidance on harnessing modern tools and technologies, including genomic selection, speed breeding, sparse testing, and so on. Additionally, the framework outlines strategies for systematically integrating novel genetic variation into elite breeding programs through pre-breeding efforts. It is adaptable across different crops and is dynamic, allowing adjustments in the breeding program based on target objectives, resource availability, and tools. The OneRice Breeding Framework is a comprehensive end-to-end framework that integrates all the components to enhance genetic gains and develop and disseminate better products faster to address food, nutrition, and income security. Consequently, the OneRice Breeding Framework is the fundamental blueprint for modern rice (<i>Oryza sativa</i>) crop breeding.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of the physiological ecology effects of global warming and recent strategies to overcome its multifaceted challenges","authors":"Azam Ranjbar,&nbsp;Ali Tajabadipour,&nbsp;Marieh Nadi","doi":"10.1002/csc2.70082","DOIUrl":"https://doi.org/10.1002/csc2.70082","url":null,"abstract":"<p>Natural resource scarcity is a major concern worldwide, and rapid population growth exacerbates limitations on resources. On the other hand, climate change leads to irregular rainfall patterns, soil erosion, water scarcity, and reduced crop yields. In this way, the evolution of agricultural ecosystems and their diversity is affected by climate change and exacerbates the issues related to food security. Sustainable production under changing climate conditions is a top priority for developed countries globally. This study examines the adaptation strategies that plants employ to combat environmental stresses. Additionally, practical strategies for managing the consequences of climate change are reviewed. All aspects of food security are considered simultaneously, aiding in the development of effective policies to ensure food security.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maturity type and flowering date instability among serradella (Ornithopus spp.) cultivars","authors":"Richard J. Simpson,&nbsp;Suzanne P. Boschma,&nbsp;Daniel R. Kidd,&nbsp;Matthew T. Newell,&nbsp;Adam Stefanski,&nbsp;Rebecca E. Haling,&nbsp;Megan H. Ryan,&nbsp;Richard C. Hayes,&nbsp;Laura Goward","doi":"10.1002/csc2.70083","DOIUrl":"https://doi.org/10.1002/csc2.70083","url":null,"abstract":"<p>Grass-legume pastures on acid soils of the temperate/Mediterranean climatic zone (∼29 million ha) of southern Australia are based almost entirely on a single annual legume (subterranean clover; <i>Trifolium subterraneum</i> L.). Serradellas (<i>Ornithopus</i> spp.) are options for legume diversification. We assessed maturity type among current serradella cultivars to evaluate whether widespread use is possible. Cultivars of yellow (<i>Ornithopus compressus</i> L.), French (<i>Ornithopus sativus</i> Brot.), and slender serradella [<i>Ornithopus pinnatus</i> (Mill.) Druce], and five subterranean clover control cultivars were sown at four sites in late autumn 2017, and in early and late autumn 2018. We determined the date at which 50% of the plants representing each cultivar had produced their first flower. Maturity type (e.g., <i>early</i>-season, <i>mid</i>-season, and <i>late</i>-season) was determined by comparison with the clover controls. Serradella cultivars ranked consistently by median flowering date within sites in both years, but the maturity type of several cultivars differed between sites. Gaps in the maturity range of serradellas needed for southern Australia included <i>very</i>-<i>late</i> maturing yellow serradellas, and <i>very</i>-<i>early</i>, <i>late</i>, and <i>very</i>-<i>late</i> hardseeded French serradellas. Unlike the clovers, many serradellas did not flower at the same time in spring when sown at different times in autumn. This was of similar concern to gaps in the maturity-type range because unstable flowering confounded maturity type classification. We argue that unstable flowering dates may also adversely impact forage quality, seed production and legume persistence. New serradella cultivars should exhibit stable flowering dates to support their adaptation in grasslands where the commencement of autumn rainfall is highly variable.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/csc2.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A diagnostic marker for the Hessian fly resistance gene H13 in wheat","authors":"Xiao-Ting Xu,&nbsp;Xuming Liu,&nbsp;Ming-Shun Chen,&nbsp;Paul St. Amand,&nbsp;Lanfei Zhao,&nbsp;Mohamed Mergoum,&nbsp;Mary J. Guttieri,&nbsp;Guihua Bai","doi":"10.1002/csc2.70070","DOIUrl":"https://doi.org/10.1002/csc2.70070","url":null,"abstract":"<p>Hessian fly [HF; Mayetiola destructor (Say)] is a serious pest of wheat in the United States, and growing HF-resistant wheat cultivars is an effective approach to control the pest. <i>H13</i> from <i>Aegilops tauschii</i> is a resistance gene on chromosome 6D that shows high resistance to multiple HF biotypes; however, diagnostic markers are not available for selection of the gene. This study developed two kompetitive allele-specific PCR (KASP) markers, <i>Kasp_H13_R1064*</i> and <i>Kasp_H13_R217K</i>, using the sequence of a previously reported <i>H13</i> candidate gene. Both markers showed the same segregation pattern in a Molly × Newton population and 38 lines that carry different named HF resistance genes. The resistance marker alleles were detected in Molly (<i>H13</i>), KS89WGRC03 (<i>H23</i>), and KS89WGRC04 (<i>Hwgrc4</i>) with the resistance genes all mapped on chromosome arm 6DS from <i>A. tauschii</i>. The results suggest those markers are near diagnostic for all three HF resistance genes and they are either allelic or tightly linked. <i>Kasp_H13_R1064*</i> was validated in a diversity panel from the major US wheat-growing areas and three wheat lines carrying <i>H13</i>. Further screening of wheat breeding lines from 2022 hard winter wheat (HWW) regional nurseries (RGON2022 and RPN2022; where RGON is the Regional Germplasm Observation Nursery and RPN is the Regional Performance Nursery) with <i>Kasp_H13_R1064*</i> did not detect <i>H13</i>, suggesting that <i>H13</i> has not been deployed in the current US HWW breeding programs. The resistance allele at <i>Kasp_H13_R1064*</i> was also relatively low (16.1%) in a worldwide <i>A. tauschii</i> collection. Development of the KASP marker for <i>H13</i> will facilitate deployment of the gene in wheat breeding programs.</p>","PeriodicalId":10849,"journal":{"name":"Crop Science","volume":"65 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}