发布求助
文献互助 智能选刊 最新文献

Current opinion in biotechnology最新文献

筛选
英文 中文
Recent developments in terpenoid biosynthesis for sustainable biofuels: from bottlenecks to emerging convergent technologies 用于可持续生物燃料的萜类生物合成的最新进展：从瓶颈到新兴融合技术
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-22 DOI: 10.1016/j.copbio.2026.103441
Zhe Zhao , Weihan Chen , Hongwei Yu , Lidan Ye
{"title":"Recent developments in terpenoid biosynthesis for sustainable biofuels: from bottlenecks to emerging convergent technologies","authors":"Zhe Zhao ,&nbsp;Weihan Chen ,&nbsp;Hongwei Yu ,&nbsp;Lidan Ye","doi":"10.1016/j.copbio.2026.103441","DOIUrl":"10.1016/j.copbio.2026.103441","url":null,"abstract":"<div><div>Terpenoid-based biofuels represent a sustainable alternative to fossil fuels with superior energy densities and combustion properties. However, achieving industrial-scale production requires overcoming multiple bottlenecks: heterologous enzyme incompatibility, metabolic flux imbalance, product toxicity, and economic viability. This review synthesizes recent breakthroughs in addressing these challenges through enzyme engineering, metabolic rewiring, host tolerance enhancement, and feedstock utilization. Simultaneously, the field is positioned at a critical juncture where convergent technologies — generative artificial intelligence for protein discovery, synthetic organelles via liquid–liquid phase separation, and engineering of non-natural terpenoid scaffolds (C₁₁, C₁₆) — promise transformative advances. This review provides a roadmap integrating these emerging capabilities to advance terpenoid-based biofuels toward commercial viability.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103441"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond the model: data infrastructure as the foundation for autonomous virtual laboratories 超越模型：数据基础设施作为自主虚拟实验室的基础
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-13 DOI: 10.1016/j.copbio.2025.103434
Lea M Sommer, Teddy Groves, Alberto Santos
{"title":"Beyond the model: data infrastructure as the foundation for autonomous virtual laboratories","authors":"Lea M Sommer,&nbsp;Teddy Groves,&nbsp;Alberto Santos","doi":"10.1016/j.copbio.2025.103434","DOIUrl":"10.1016/j.copbio.2025.103434","url":null,"abstract":"<div><div>Artificial intelligence (AI) and machine learning are reshaping biotechnology, but their impact is still constrained by inadequate data infrastructure. Although recent advances have focused on model architectures, data quality, standardization, and interoperability remain the main bottlenecks. A data-centric approach that strengthens data practices across the design-build-test-learn cycle is needed to advance AI-enabled biomanufacturing. In this review, we argue that curated repositories, consistent metadata, real-time validation, and efficient learning strategies are essential prerequisites. We highlight the manual test-to-learn ingestion step as a critical source of latency that can be mitigated through semantic standardization and structured data collection. By addressing these data-centric limitations, the field can move toward reliable, scalable, and ultimately autonomous virtual laboratories that adhere to the Findable, Accessible, Interoperable, Reusable principles.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103434"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145972879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in tailoring camelina and pennycress oilseeds for specialty metabolites 针对特殊代谢物定制亚麻荠和月桂菜籽的研究进展
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-03 DOI: 10.1016/j.copbio.2025.103422
Tingyuan Xiao, Timothy P Durrett
{"title":"Advances in tailoring camelina and pennycress oilseeds for specialty metabolites","authors":"Tingyuan Xiao,&nbsp;Timothy P Durrett","doi":"10.1016/j.copbio.2025.103422","DOIUrl":"10.1016/j.copbio.2025.103422","url":null,"abstract":"<div><div>Oilseeds provide a renewable platform to produce lipids and other valuable biomolecules. While conventional crops accumulate a limited set of fatty acids, advances in synthetic biology now enable exogenous pathways to expand oil diversity. The oilseeds camelina (<em>Camelina sativa</em>) and pennycress (<em>Thlaspi arvense</em>) have emerged as powerful platforms for this work due to their efficient transformation methods. Recent breakthroughs have come from genome-editing rewiring of endogenous lipid metabolism to remove competing pathways, which, when combined with bioprospecting to identify more efficient enzymes, delivers the greatest gains in product yield. Overcoming challenges such as achieving cell-type–specific expression and developing scalable strategies for pathway gene expression control will ensure these crops realize their potential as versatile platforms for next-generation bioproducts.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103422"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering whole-cell catalysts to use plastic waste as a feedstock 设计全细胞催化剂，使用塑料废物作为原料
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-23 DOI: 10.1016/j.copbio.2026.103443
Jose I Jiménez , Catalina Cruañas Pániker , Brooke H Wain
{"title":"Engineering whole-cell catalysts to use plastic waste as a feedstock","authors":"Jose I Jiménez ,&nbsp;Catalina Cruañas Pániker ,&nbsp;Brooke H Wain","doi":"10.1016/j.copbio.2026.103443","DOIUrl":"10.1016/j.copbio.2026.103443","url":null,"abstract":"<div><div>The extensive production, durability, and waste mismanagement of plastic polymers have led to a highly concerning environmental problem. Recycling methods aim to reduce the amount of plastic pollution, and among them, biological processes have emerged as an interesting alternative for the management of plastic waste that is difficult to collect or can not be recycled by other methods. While there has been significant progress in the field, in particular related to the enzymatic hydrolysis of polyesters, most biological methods rely on the use of enzymes <em>in vitro</em>, using collected plastics. In this review, we explore the status of technologies using whole-cell catalysts that could be used for <em>in vivo</em> upcycling of plastic waste — with plastic becoming a microbial feedstock — and for the development of biodegradation strategies in relevant environments. We have identified a number of barriers related to polymer bioavailability, enzyme activity and secretion, and the use of strains and microbial communities that need to be overcome to materialize a much-needed solution to plastic pollution.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103443"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent progress in plant genome engineering: from large insertions to chromosome number changes 植物基因组工程的最新进展：从大插入到染色体数目的变化
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-08 DOI: 10.1016/j.copbio.2025.103426
Seda Yaşar, Fabienne Gehrke, Niklas Capdeville, Holger Puchta
{"title":"Recent progress in plant genome engineering: from large insertions to chromosome number changes","authors":"Seda Yaşar,&nbsp;Fabienne Gehrke,&nbsp;Niklas Capdeville,&nbsp;Holger Puchta","doi":"10.1016/j.copbio.2025.103426","DOIUrl":"10.1016/j.copbio.2025.103426","url":null,"abstract":"<div><div>The adaptation of the CRISPR/Cas system as a biotechnological tool has enabled a wide spectrum of targeted genome modifications. Whereas earlier approaches focused on small sequence changes, recent years have seen a shift toward larger-scale alterations. Advances in homology-directed gene targeting now enable efficient, scar-free kilobase insertions, while combining nuclease-deficient Cas effectors with recombinases or transposases allows the integration of much larger sequences. Prime editing further expands this scope, enabling inversions, replacements, and deletions spanning hundreds of kilobases to several megabases. More recently, genome engineering has reached a new stage with chromosome fission and fusion, demonstrating the feasibility of controlled karyotype restructuring. Together, these advances open new opportunities for crop improvement, from establishing reproductive barriers and mimicking evolutionary processes to trait stacking on Plant Artificial Chromosomes.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103426"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145920970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Model acetogens as chassis for CO2-driven bioproduction 模拟二氧化碳驱动生物生产的二氧化碳源
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-09 DOI: 10.1016/j.copbio.2025.103423
Karen Rodriguez , Jitendra Joshi , Chris Greening , Esteban Marcellin
{"title":"Model acetogens as chassis for CO2-driven bioproduction","authors":"Karen Rodriguez ,&nbsp;Jitendra Joshi ,&nbsp;Chris Greening ,&nbsp;Esteban Marcellin","doi":"10.1016/j.copbio.2025.103423","DOIUrl":"10.1016/j.copbio.2025.103423","url":null,"abstract":"<div><div>Microbes play a pivotal role in the Earth’s carbon cycle, regulating greenhouse gas fluxes by emitting, fixing and transforming CO<sub>2</sub>. Among them, acetogens stand out for their ability to fix CO<sub>2</sub> through the Wood–Ljungdahl pathway, an ancient, highly energy-efficient route to acetyl-CoA that operates at thermodynamic limits. By coupling hydrogen (H<sub>2</sub>) or carbon monoxide oxidation to CO<sub>2</sub> fixation, acetogens conserve energy while generating biomass and valuable products such as ethanol and acetate. These features position them as promising microbial cell factories for sustainable bioproduction via gas fermentation. Recent advances in metabolic engineering and synthetic biology have expanded the production spectrum of acetogens, enabling production of platform chemicals at lab-to-commercial scale. Yet, CO<sub>2</sub>-only bioconversion remains energetically challenging compared to syngas-based applications, requiring innovative solutions in strain development, bioprocess optimisation and integration of renewable energy sources. This review highlights the central role of model acetogens in anaerobic CO<sub>2</sub> conversion, covering their metabolic capabilities, strain development and emerging bioprocess strategies to unlock their potential for low-carbon biomanufacturing.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103423"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design principles for adaptive and evolving engineered living materials 适应性和进化工程生物材料的设计原则。
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-03 DOI: 10.1016/j.copbio.2025.103397
Yifan Cui , Mark W Tibbitt , Timothy K Lu , Tzu-Chieh Tang
{"title":"Design principles for adaptive and evolving engineered living materials","authors":"Yifan Cui ,&nbsp;Mark W Tibbitt ,&nbsp;Timothy K Lu ,&nbsp;Tzu-Chieh Tang","doi":"10.1016/j.copbio.2025.103397","DOIUrl":"10.1016/j.copbio.2025.103397","url":null,"abstract":"<div><div>Engineered living materials (ELMs) combine living cells, typically microorganisms, such as bacteria, yeasts, or filamentous fungi, with structural carrier matrices to form systems capable of sensing, growth, and self-repair. Most current designs emphasize programming the microbes to render otherwise static materials functional. A less explored dimension is leveraging reciprocal microbial–material interactions themselves to engineer adaptive and evolving living materials as integrated systems. Achieving such dynamic behavior requires understanding how support matrices influence microbial behavior and how cells, in turn, reshape material properties over time. This review outlines key modes of cell–material interactions as a framework for expanding the functional toolbox of ELMs and for creating sustainable and programmable materials that respond to their environments and evolve.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103397"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Windows of operation as qualitative early-stage design tool for microfluidic (single-cell) cultivations 操作窗口作为微流体（单细胞）培养的定性早期设计工具
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-13 DOI: 10.1016/j.copbio.2025.103401
Yannick Scholz, Boris Yermakov, Alexander Grünberger
{"title":"Windows of operation as qualitative early-stage design tool for microfluidic (single-cell) cultivations","authors":"Yannick Scholz,&nbsp;Boris Yermakov,&nbsp;Alexander Grünberger","doi":"10.1016/j.copbio.2025.103401","DOIUrl":"10.1016/j.copbio.2025.103401","url":null,"abstract":"<div><div>Microfluidic cultivation systems have transformed the study of cellular physiology by enabling high spatial and temporal analysis under precisely controlled environmental conditions. However, the successful application of these systems is hindered by technical challenges, including the lack of systematic characterization of key operational parameters and their interdependencies, which limits experimental reproducibility within a given setup and hampers the rational design of new systems. Here, we propose adapting ‘Windows of Operation’ — a framework originating from bioprocess engineering to visualize how different parameters define design limits — as a qualitative operational design tool for microfluidic cultivation, here further denoted as microfluidic window of operation (MWO). Through selected case studies, we demonstrate how defining MWOs can guide the identification and optimization of key experimental parameters. This provides a foundation for robust experimental design that links device function with operating parameters, thereby advancing feasibility, robustness, and comparability, while minimizing experimental bias.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103401"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The systems biology of sleep: toward integrative understanding of molecular and circuit-based mechanisms of sleep 睡眠的系统生物学：迈向对睡眠的分子和电路机制的综合理解。
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-10 DOI: 10.1016/j.copbio.2025.103427
Hiroyuki J Kanaya , Koji L Ode , Hiroki R Ueda
{"title":"The systems biology of sleep: toward integrative understanding of molecular and circuit-based mechanisms of sleep","authors":"Hiroyuki J Kanaya ,&nbsp;Koji L Ode ,&nbsp;Hiroki R Ueda","doi":"10.1016/j.copbio.2025.103427","DOIUrl":"10.1016/j.copbio.2025.103427","url":null,"abstract":"<div><div>Sleep, a universal biological phenomenon, is regulated by multiscale processes, from molecular mechanisms to cellular networks. While the underlying mechanisms, particularly those governing sleep homeostasis, were poorly understood, recent technological breakthroughs have facilitated the identification of molecular and circuit-based mechanisms. Advances in mouse genetics, including next-generation genetics that bypass the need for crossing and postnatal gene knockout methods, enable the comprehensive identification of molecular components for sleep regulation by combining them with noninvasive, large-scale sleep measurements. Elucidated mechanisms include Ca<sup>2+</sup>-related and protein kinase/phosphatase-mediated signaling, supporting the phosphorylation hypothesis of sleep. The molecular signaling forms ‘cellular sleepiness’ in sleep regulatory neurons to modulate neuronal activity. These integrated understandings of multiscale mechanisms will lead to a system-level understanding of sleep regulation.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103427"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular diagnostics for infectious disease and cancer based on glass capillary nanopore sensing 基于玻璃毛细管纳米孔传感的传染病和癌症分子诊断。
IF 7 2区 工程技术
Current opinion in biotechnology Pub Date : 2026-02-01 Epub Date: 2025-11-29 DOI: 10.1016/j.copbio.2025.103394
Ryogo Higashi , Izumi Shibayama , Jiajue Ji , Tao Ren , Iiro Kiiski , Nanami Takeuchi , Ryuji Kawano
{"title":"Molecular diagnostics for infectious disease and cancer based on glass capillary nanopore sensing","authors":"Ryogo Higashi ,&nbsp;Izumi Shibayama ,&nbsp;Jiajue Ji ,&nbsp;Tao Ren ,&nbsp;Iiro Kiiski ,&nbsp;Nanami Takeuchi ,&nbsp;Ryuji Kawano","doi":"10.1016/j.copbio.2025.103394","DOIUrl":"10.1016/j.copbio.2025.103394","url":null,"abstract":"<div><div>Glass capillary nanopores have been widely used for resistive pulse sensing and ionic current rectification-based nanopore sensing due to their excellent mechanical properties, simple fabrication, easy surface modification, and low production cost. These nanopores have demonstrated great potential in detecting diverse biomarkers, including nucleic acids, proteins, cell vesicles, viruses, and bacteria. Here, we highlight recent advances in molecular diagnostics using glass capillary nanopores, especially focusing on the detection of infectious disease pathogens and cancer-associated microRNAs. By integrating precisely sized glass nanopipette-based nanopores with nucleic acid amplification techniques and well-designed probes, this sensing platform has emerged as a promising method for liquid biopsy from patient body fluids. In consideration of recent research on molecular diagnostics and the difficulties with glass capillary nanopipettes, we also discuss the future direction of molecular diagnostics using this tool.</div></div>","PeriodicalId":10833,"journal":{"name":"Current opinion in biotechnology","volume":"97 ","pages":"Article 103394"},"PeriodicalIF":7.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145647698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书