提高生物技术工艺效率的热力学工具：以一氧化碳生产聚（3-羟基丁酸）为例。

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology Pub Date : 2024-10-01 DOI:10.1016/j.copbio.2024.103212

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引用次数: 0

摘要

现代生物技术需要整合多个学科，其中热力学是一个重要学科。生物技术中经常使用的实验方法，如重新连接代谢网络或在工程环境中培养微生物，都可以从热力学工具的应用中获益。本文概述了几种有助于设计和优化生物技术过程的热力学工具，并展示了这些工具在利用一氧化碳（CO）生产聚-（3-羟基丁酸）（PHB）过程中的潜在应用。我们讨论了这些工具如何帮助设计代谢工程策略、计算预期产量、评估目标转化的热力学可行性、识别潜在的热力学瓶颈以及选择基因工程目标。虽然我们使用从 CO 生产 PHB 的具体实例来说明这些工具，但它们也可应用于其他底物和产品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide

Modern biotechnology requires the integration of several disciplines, with thermodynamics being a crucial one. Experimental approaches frequently used in biotechnology, such as rewiring of metabolic networks or culturing of micro-organisms in engineered environments, can benefit from the application of thermodynamic tools. In this paper, we provide an overview of several thermodynamic tools that are useful for the design and optimization of biotechnological processes, and we demonstrate their potential application in the production of poly-(3-hydroxybutyrate) (PHB) from carbon monoxide (CO). We discuss how these tools can aid in the design of metabolic engineering strategies, the calculation of expected yields, the assessment of the thermodynamic feasibility of the targeted conversions, the identification of potential thermodynamic bottlenecks, and the selection of genetic engineering targets. Although we illustrate these tools using the specific example of PHB production from CO, they can be applied to other substrates and products.

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来源期刊

Current opinion in biotechnology 工程技术-生化研究方法

CiteScore

16.20

自引率

2.60%

发文量

226

审稿时长

4-8 weeks

期刊介绍： Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time. As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows. COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.