Methane to bioproducts: unraveling the potential of methanotrophs for biomanufacturing

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology Pub Date : 2024-10-04 DOI:10.1016/j.copbio.2024.103210

Justin N Tan , Keshav Ratra , Steven W Singer , Blake A Simmons , Shubhasish Goswami , Deepika Awasthi

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引用次数: 0

Abstract

With the continuous increase in the world population, anthropogenic activities will generate more waste and create greenhouse gases such as methane, amplifying global warming. The biological conversion of methane into biochemicals is a sustainable solution to sequester and convert this greenhouse gas. Methanotrophic bacteria fulfill this role by utilizing methane as a feedstock while manufacturing various bioproducts. Recently, methanotrophs have made their mark in industrial biomanufacturing. However, unlike glucose-utilizing model organisms such as Escherichia coli and Saccharomyces cerevisiae, methanotrophs do not have established transformation methods and genetic tools, making these organisms challenging to engineer. Despite these challenges, recent advancements in methanotroph engineering demonstrate great promise, showcasing these C1-carbon-utilizing microbes as prospective hosts for bioproduction. This review discusses the recent developments and challenges in strain engineering, biomolecule production, and process development methodologies in the methanotroph field.

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从甲烷到生物产品：揭示甲烷营养体在生物制造方面的潜力。

随着世界人口的不断增加，人类活动将产生更多的废物，并产生甲烷等温室气体，加剧全球变暖。通过生物转化将甲烷转化为生化物质是封存和转化这种温室气体的可持续解决方案。养甲烷细菌利用甲烷作为原料，制造各种生物产品，从而发挥了这一作用。最近，甲烷营养细菌在工业生物制造领域大显身手。然而，与大肠杆菌和酿酒酵母等利用葡萄糖的模式生物不同，甲烷营养菌没有成熟的转化方法和遗传工具，因此这些生物的工程设计具有挑战性。尽管存在这些挑战，但最近在甲烷营养体工程学方面取得的进展展示了巨大的前景，表明这些 C1 碳利用微生物有望成为生物生产的宿主。本综述讨论了甲烷营养体领域在菌种工程、生物大分子生产和工艺开发方法方面的最新进展和挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in biotechnology 工程技术-生化研究方法

CiteScore

16.20

自引率

2.60%

发文量

226

审稿时长

4-8 weeks

期刊介绍： Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time. As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows. COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.