Current Medical Science最新文献

{"title":"A Simple Modification Results in a Significant Improvement in Measuring the Size of Extracellular Vesicles.","authors":"Xiao-Jun Liu, Zhen-Sheng Ma, Yan Li, Tai-Bing Fan, Zhen-Wei Ge, Zhi-Jun Ou, Jing-Song Ou","doi":"10.1007/s11596-025-00045-z","DOIUrl":"https://doi.org/10.1007/s11596-025-00045-z","url":null,"abstract":"<p><strong>Objective: </strong>Size distribution is an important biophysical property of extracellular vesicles (EVs). EVs include small EVs (s-EVs) and large EVs (l-EVs) by size. Differential ultracentrifugation (dUC) is widely used to separate EVs from biofluids, but it can precipitate large impurity particles. Dynamic light scattering (DLS) is a simple and fast method for analyzing the size distribution of EVs. However, this approach is nonideal for heterogeneous and polydisperse samples since a small quantity of large impurity particles can markedly disturb the DLS results. Here, we developed a simple method to improve the reliability of DLS measurements.</p><p><strong>Methods: </strong>Plasma was obtained from 13 volunteers. The plasma was first processed by dUC to obtain crude l-EVs. The crude l-EVs were filtered with syringe filters (pore size of 1 μm and membrane material of hydrophilic polyvinylidene fluoride (PVDF)) to remove large impurity particles from l-EVs. The size distributions of the crude l-EVs and filtered l-EVs were measured via DLS.</p><p><strong>Results: </strong>After the samples were filtered, the coefficients of variation of the hydrodynamic radius and Peak 1 intensity of the filtered l-EVs decreased from 20.39% (12.76-28.96%) and 20.44% (14.58-28.32%) to 3.05% (1.79-4.72%) and 3.43% (1.76-5.88%), respectively, compared with those of the crude l-EVs.</p><p><strong>Conclusion: </strong>These findings suggest that filtration can effectively separate circulating l-EVs in plasma to remove large impurity particles and make samples suitable for characterization by DLS. Our findings provide a simple method to improve precision via DLS to measure the size distribution of EVs.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":"45 2","pages":"244-252"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Retrograde Pubic Ramus Intramedullary Nails and Percutaneous Cannulated Screws in Treating Anterior Pelvic Ring Fractures: A Retrospective Cohort Study.","authors":"En-Zhi Yin, Xue-Feng Yuan, Yang-Xing Luo, Peng-Hui Xiang, Li He, Yi-Liu Liao, Cheng-la Yi","doi":"10.1007/s11596-025-00044-0","DOIUrl":"10.1007/s11596-025-00044-0","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical outcomes of retrograde pubic ramus intramedullary nail (RPRIN) and percutaneous cannulated screw (PCS) in the treatment of anterior pelvic ring fractures (APRFs).</p><p><strong>Methods: </strong>This retrospective cohort study included 45 patients with APRFs treated between February 2019 and October 2022 in our trauma center. Patients were divided into two groups based on the surgical method: 20 received RPRIN fixation, and 25 received PCS fixation. Key variables including operation time, fluoroscopic time, blood loss, and postoperative complications were analyzed. Fracture reduction quality was assessed using the Matta score system, and pelvic functional recovery was evaluated using the Majeed score system at the final follow-up. Quantitative variables were compared using the independent sample t test, while categorical variables were analyzed using Chi-square and Fisher's exact tests.</p><p><strong>Results: </strong>The RPRIN group had significantly shorter operation time (36.3 ± 5.6 min vs. 49.5 ± 6.9 min, P < 0.01), fluoroscopic time (32.0 ± 2.8 s vs. 48.4 ± 3.6 s, P < 0.01), and less blood loss (20.4 ± 7.6 mL vs. 34.0 ± 5.7 mL, P < 0.01) than the PCS group. Fracture reduction quality (Matta outcome) and pelvic functional recovery (Majeed outcome) were comparable between the two groups (P > 0.05). No significant complications were reported in either group.</p><p><strong>Conclusions: </strong>Both RPRIN and PCS are effective for treating APRFs. However, RPRIN offers distinct advantages by reducing operation time, fluoroscopic time, and blood loss, making it a more efficient and less invasive option. Further multicenter studies and biomechanical analyses are warranted to confirm these findings.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"341-348"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progression from Initial Lesions to Type B Aortic Dissection: A Patient-Specific Study of Computational Fluid Dynamics Models with Follow-up Data.","authors":"Yue-Ying Pan, Zhi-Yue Guan, Chen-Wei Li, Han-Xiong Guan","doi":"10.1007/s11596-025-00006-6","DOIUrl":"10.1007/s11596-025-00006-6","url":null,"abstract":"<p><strong>Background and objective: </strong>The natural history of type B aortic intramural hematoma (IMH) is highly heterogeneous. A computational fluid dynamics (CFD) model can be utilized to calculate a range of data pertinent to flow dynamics, including flow rates, blood velocity, pressure, and wall shear stress. This study presents a series of CFD simulations that model the dynamic progression from type B aortic IMH to false lumen formation.</p><p><strong>Methods: </strong>A 66-year-old male patient presenting with chest and back pain underwent aortic computed tomography angiography (CTA), and a 3D patient-specific model was constructed. To evaluate the hemodynamic environment, the velocity, pressure, time-averaged wall shear stress (TAWSS), and oscillatory shear index (OSI) were calculated.</p><p><strong>Results: </strong>A modest quantity of slow flow and recirculation flow was observed in the vicinity of the ulcer-like protrusion (ULP). During the formation of the false lumen, low-velocity blood flow entered the false lumen and resulted in vortex flow. ULPs were located in the region with higher TAWSS, and some high OSIs were found on the ULPs.</p><p><strong>Conclusion: </strong>This preliminary study suggests a potential association between the TAWSS or OSI and progression from type B aortic IMH to aortic dissection.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"373-381"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning-Based Diagnostic Model for Parkinson's Disease Using Handwritten Spiral and Wave Images.","authors":"K Aditya Shastry","doi":"10.1007/s11596-025-00017-3","DOIUrl":"10.1007/s11596-025-00017-3","url":null,"abstract":"<p><strong>Objective: </strong>To develop and validate a deep neural network (DNN) model for diagnosing Parkinson's Disease (PD) using handwritten spiral and wave images, and to compare its performance with various machine learning (ML) and deep learning (DL) models.</p><p><strong>Methods: </strong>The study utilized a dataset of 204 images (102 spiral and 102 wave) from PD patients and healthy subjects. The images were preprocessed using the Histogram of Oriented Gradients (HOG) descriptor and augmented to increase dataset diversity. The DNN model was designed with an input layer, three convolutional layers, two max-pooling layers, two dropout layers, and two dense layers. The model was trained and evaluated using metrics such as accuracy, sensitivity, specificity, and loss. The DNN model was compared with nine ML models (random forest, logistic regression, AdaBoost, k-nearest neighbor, gradient boost, naïve Bayes, support vector machine, decision tree) and two DL models (convolutional neural network, DenseNet-201).</p><p><strong>Results: </strong>The DNN model outperformed all other models in diagnosing PD from handwritten spiral and wave images. On spiral images, the DNN model achieved accuracies of 41.24% over naïve Bayes, 31.24% over decision tree, and 27.9% over support vector machine. On wave images, the DNN model achieved accuracies of 40% over naïve Bayes, 36.67% over decision tree, and 30% over support vector machine. The DNN model demonstrated significant improvements in sensitivity and specificity compared to other models.</p><p><strong>Conclusions: </strong>The DNN model significantly improves the accuracy of PD diagnosis using handwritten spiral and wave images, outperforming several ML and DL models. This approach offers a promising diagnostic tool for early PD detection and provides a foundation for future work to incorporate additional features and enhance detection accuracy.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"206-230"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scoring the Clinical Application of a Novel Scale in a Hybrid Operating Room for Neurosurgery.","authors":"Wei-Yu Sun, Yu Feng, Jin Yu, Ting-Bao Zhang, Yi-Hui Ma, Kun-Xian Zhang, Xi-Qian Gu, Min Niu, Xiang Li, Jin-Cao Chen, Wen-Yuan Zhao","doi":"10.1007/s11596-025-00015-5","DOIUrl":"10.1007/s11596-025-00015-5","url":null,"abstract":"<p><strong>Objective: </strong>The lack of clarity regarding the application performance of a hybrid operating room (HOR) and the uncertainty of surgical scheduling often lead to its inefficient application. This study aimed to review the clinical application of our neurosurgical HOR and propose a scale to score cases clearly.</p><p><strong>Methods: </strong>We reviewed the operating procedures and duration of stay in 1865 HOR cases. The actual procedures of each case were summarized into 5 application types, and numerical assignment was used to distinguish the dependence of each type on our HOR: surgical procedures combined with interventional procedures (4 points, the highest dependence), surgical procedures combined with imaging procedures (3 points), interventional procedures (2 points), imaging procedures (1 point), and surgical procedures (0 points, the lowest dependence).</p><p><strong>Results: </strong>A novel scale that could score 1865 cases into those 5 grades was developed. The percentages by grade were as follows: 4 points, 4.24%; 3 points, 4.88%; 2 points, 20.75%; 1 point, 69.38%; and 0 points, 0.75%. The cumulative usage time was 4241.9 h, the duration of which was as follows: 4 points, 16.17%; 3 points, 15.50%; 2 points, 31.32%; 1 point, 35.62%; and 0 points, 1.39%.</p><p><strong>Conclusions: </strong>The HOR serves as a multifunctional room to treat neurosurgical diseases. The scale helps to quickly prioritize cases that rely more on HOR, providing guidelines for surgical scheduling. Although our HOR is unsuitable for emergency cases, it clearly shows the application performance of our HOR to provide a reference for promoting its efficient application.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"349-362"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen Detection in Spinal Infections: Next-Generation Sequencing Versus Conventional Microbiological Methods.","authors":"Khan Akhtar Ali, Ling-Xiao He, Fang Gao, Ze-An Xia, Hui Huang, Heng Zeng, Wei-Hua Hu","doi":"10.1007/s11596-025-00040-4","DOIUrl":"10.1007/s11596-025-00040-4","url":null,"abstract":"<p><strong>Objective and background: </strong>Early and accurate diagnosis of spinal infections, including spinal tuberculosis, is pivotal for effective treatment but remains challenging. This study aims to assess the diagnostic yield of metagenomic next-generation sequencing (mNGS) compared with that of conventional microbiological tests (CMTs) in identifying pathogens associated with spinal pathologies, with a special focus on infections leading to surgical interventions.</p><p><strong>Methods: </strong>We enrolled 85 patients who underwent spinal surgery, comprising 63 patients with clinically diagnosed spinal infections, including patients with spinal tuberculosis, and 22 patients with noninfectious spinal conditions. The procedures involved irrigation and debridement for persistent wound drainage, with subsequent DNA extraction from plasma and joint fluid for mNGS and CMT analysis.</p><p><strong>Results: </strong>Significantly increased C-reactive protein (CRP) levels were observed in patients with infections. The mNGS approach showed greater diagnostic sensitivity (92.06%) for detecting pathogens, including Mycobacterium tuberculosis, than did CMTs (36.51%). Despite its low specificity, mNGS had considerable negative predictive value (70.59%), underscoring its utility in ruling out infections.</p><p><strong>Conclusions: </strong>The mNGS offers superior sensitivity over CMTs in the diagnosis of a variety of spinal infections, notably spinal tuberculosis. This study highlights the potential of mNGS in enhancing the diagnosis of complex spinal infections, thereby informing targeted treatment strategies.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"331-340"},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143728964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Stereotactic Injection of shRNA GSK-3β-AAV Promotes Axonal Regeneration after Spinal Cord Injury.","authors":"Yu-Chao Zuo, Nan-Xiang Xiong, Hong-Yang Zhao","doi":"10.1007/s11596-025-00012-8","DOIUrl":"10.1007/s11596-025-00012-8","url":null,"abstract":"","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"154-155"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avocado Oil: Recent Advances in Its Anti-diabetic Potential.","authors":"Dorji Drakpa, Taniya Paul, Sukriti Chakrabarty, Karma Jigdrel, Prasun Mukherjee, Jeena Gupta","doi":"10.1007/s11596-025-00010-w","DOIUrl":"10.1007/s11596-025-00010-w","url":null,"abstract":"<p><strong>Background: </strong>Diet plays a crucial role in insulin resistance and diabetes, with high-fat and high-carbohydrate diets being major contributors. However, the type of fat consumed is critical, as different fatty acids impact insulin sensitivity differently.</p><p><strong>Objective: </strong>This review examines the potential benefits of avocado oil, which is rich in monounsaturated fatty acids (MUFAs), in improving glycaemic control and lipid metabolism. It also explores variations in avocado oil composition across different avocado cultivars.</p><p><strong>Methods: </strong>Experimental and clinical studies were analysed to assess the metabolic effects of avocado oil. The impact of MUFAs on insulin sensitivity, lipid profiles, and metabolic health was reviewed, alongside the influence of fruit quality, maturity, and cultivar differences.</p><p><strong>Results: </strong>Avocado oil may enhance glycaemic control and lipid metabolism, benefiting individuals with diabetes and hypercholesterolemia. However, its composition varies significantly across avocado variants such as Hass, Reed, Ettinger, and Fuerte, influencing its therapeutic properties.</p><p><strong>Conclusion: </strong>Avocado oil's high MUFA content offers promising metabolic benefits. Further research is needed to standardize its therapeutic application, considering cultivar-dependent variations in composition.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"11-24"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Helicobacter pylori Eradication on Clinical and Laboratory Parameters in Non-alcoholic Fatty Liver Disease Patients: A Systematic Review and Meta-analysis of Randomized Controlled Trials.","authors":"Fouad Jaber, Saqr Alsakarneh, Azizullah Beran, Tala Alsharaeh, Ahmed-Jordan Salahat, Abdelrahman Abdelshafi, Islam Mohamed, Willie Johnson, Khaled Elfert, Mohammad Jaber, Mohammad Almeqdadi, Mohamed Ahmed, Hassan Ghoz, Wendell K Clarkston, John H Helzberg","doi":"10.1007/s11596-025-00001-x","DOIUrl":"10.1007/s11596-025-00001-x","url":null,"abstract":"<p><strong>Objective: </strong>Helicobacter pylori (HP) infection is associated with non-alcoholic fatty liver disease (NAFLD) and insulin resistance; however, the correlation between HP eradication and NAFLD remains controversial. This systematic review and meta-analysis examined the effect of HP treatment on clinical and laboratory parameters in NAFLD patients.</p><p><strong>Methods: </strong>We conducted a literature search of the PubMed, Embase, Scopus, and Web of Science databases through September 2023 for randomized controlled trials (RCTs) examining the effect of HP treatment on NAFLD patients versus lifestyle changes alone. The primary outcome was the change in steatosis parameters. The secondary endpoints were changes in anthropometric parameters, inflammatory markers (TNF-α), and metabolic parameters (fasting blood glucose, homeostasis model assessment of insulin resistance, AST/ALT, and lipid profile). The random effects model was used to calculate the standardized mean difference (SMD) with associated 95% confidence intervals (CIs) for our desired outcome.</p><p><strong>Results: </strong>Four RCTs met our inclusion criteria. A total of 453 patients were included (mean age 42.8 years, 58.5% males), 228 (50.3%) of whom were in the HP eradication group and 225 (49.7%) of whom were in the lifestyle modification group. Compared with lifestyle modification alone, HP eradication had a significant effect on reducing liver steatosis and TNF-α levels (SMD: - 0.9; 95% CI - 14.67, - 3.82, I² = 0% and SMD: - 6.3; 95% CI - 9.04, - 3.56, I² = 0%, respectively). No significant effect on other metabolic parameters was found.</p><p><strong>Conclusions: </strong>HP eradication significantly reduced liver steatosis and TNF-α levels in NAFLD patients. However, HP eradication did not significantly affect other metabolic indices compared to lifestyle changes alone.</p>","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"1-10"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to: Electroacupuncture Alleviates Memory Deficits in APP/PS1 Mice by Targeting Serotonergic Neurons in Dorsal Raphe Nucleus.","authors":"Chao-Chao Yu, Xiao-Fei Wang, Jia Wang, Chu Li, Juan Xiao, Xue-Song Wang, Rui Han, Shu-Qin Wang, Yuan-Fang Lin, Li-Hong Kong, Yan-Jun Du","doi":"10.1007/s11596-025-00014-6","DOIUrl":"10.1007/s11596-025-00014-6","url":null,"abstract":"","PeriodicalId":10820,"journal":{"name":"Current Medical Science","volume":" ","pages":"156"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}