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Inheritance and fitness costs of Cry1Ab and Cry1Ac resistance in Brazilian populations of Diatraea saccharalis (Lepidoptera: Crambidae) 巴西糖翅蝗种群Cry1Ab和Cry1Ac抗性的遗传和适应代价(鳞翅目:革蛾科)
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-24 DOI: 10.1016/j.cropro.2025.107216
Juliana G. Rodrigues , Caroline Izabel R. Sakuno , Fabricio José B. Francischini , Karen Missy A. Komada , Fangneng Huang
{"title":"Inheritance and fitness costs of Cry1Ab and Cry1Ac resistance in Brazilian populations of Diatraea saccharalis (Lepidoptera: Crambidae)","authors":"Juliana G. Rodrigues ,&nbsp;Caroline Izabel R. Sakuno ,&nbsp;Fabricio José B. Francischini ,&nbsp;Karen Missy A. Komada ,&nbsp;Fangneng Huang","doi":"10.1016/j.cropro.2025.107216","DOIUrl":"10.1016/j.cropro.2025.107216","url":null,"abstract":"<div><div>The sugarcane borer, <em>Diatraea saccharalis</em> (F.), is the main insect pest on sugarcane in the Americas. Recently, transgenic <em>Bacillus thuringiensis</em> (Bt) sugarcane is planted in Brazil to control <em>D. saccharalis</em>. Resistance development is the greatest challenge for the sustainability of Bt crop biotechnology. Inheritance and fitness costs of resistance are essential information needed in developing effective insecticide resistance management (IRM) programs. In this study, multiple crossings were performed to characterize the inheritance and fitness costs of Cry1Ab and Cry1Ac resistance in two Brazilian populations (Cry1Ab_RR and Cry1Ac_RR) of <em>D. saccharalis</em>. Diet-incorporated and plant tissue bioassays showed that both the Cry1Ab and Cry1Ac resistance in the insect is inherited as a single, autosomal, and genetically incompletely recessive gene. Complementation tests showed that the Cry1Ab and Cry1Ac resistance in the two populations shared the same resistance gene. Leaf tissue bioassays suggested that the Cry1Ac sugarcane variety (CTC9001BT) tested in the study most likely meets the ‘high dose’ requirement for <em>D. saccharalis</em> as defined in the ‘high dose/refuge’ IRM strategy for Bt crops, while additional studies are needed to document the ‘high dose’ qualification for the tested Cry1Ab sugarcane variety (CTC20BT). Effective dominance levels of resistance decreased as Bt concentrations increased, suggesting that elevating Bt protein expressions in plants could be an effective strategy to make a ‘non-high dose’ plant become a ‘high dose’ plant. The Bt resistance in Cry1Ab_RR and Cry1Ac_RR was not associated with fitness costs on both non-Bt sugarcane and non-Bt corn/meridic diet. Knowledge generated from this study is useful in resistance risk assessment, monitoring, and development of IRM programs for the sustainable use of Bt sugarcane technology for pest management.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"194 ","pages":"Article 107216"},"PeriodicalIF":2.5,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple effects of indoxacarb on the soybean caterpillar Anticarsia gemmatalis 茚虫威对大豆毛虫的多重防治作用
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-22 DOI: 10.1016/j.cropro.2025.107214
Carlos Henrique Batista , Angelica Plata-Rueda , Weslei dos Santos Cunha , José Cola Zanuncio , José Eduardo Serrão , Luis Carlos Martínez
{"title":"Multiple effects of indoxacarb on the soybean caterpillar Anticarsia gemmatalis","authors":"Carlos Henrique Batista ,&nbsp;Angelica Plata-Rueda ,&nbsp;Weslei dos Santos Cunha ,&nbsp;José Cola Zanuncio ,&nbsp;José Eduardo Serrão ,&nbsp;Luis Carlos Martínez","doi":"10.1016/j.cropro.2025.107214","DOIUrl":"10.1016/j.cropro.2025.107214","url":null,"abstract":"<div><div>The caterpillar, <em>Anticarsia gemmatalis</em> is one of the most important defoliating pests of soybean crops in the Americas. Control of <em>A</em>. <em>gemmatalis</em>, is primarily achieved with chemical insecticides; however, populations of this species have developed resistance to certain chemical molecules. Therefore, insecticides with different modes of action should be evaluated to control this pest. The objective of this study was to evaluate the effects of the insecticide indoxacarb on mortality, respiration, locomotor behavior, and midgut histology in <em>A. gemmatalis</em> caterpillars. After 72 h of exposure, indoxacarb was toxic to <em>A. gemmatalis</em> caterpillars, with 87 % survival in the control group and 54 %, 38 %, 24 %, and 0 % survival in those exposed to indoxacarb at LC<sub>25</sub> (2.14 g a.i. L<sup>−1</sup>), LC<sub>50</sub> (3.08 g a.i. L<sup>−1</sup>), LC<sub>75</sub> (4.42 g a.i. L<sup>−1</sup>), and LC<sub>90</sub> (6.39 g a.i. L<sup>−1</sup>), respectively. With increasing concentrations, the locomotor behavior of <em>A. gemmatalis</em> was altered, with shorter walking distances and longer resting time avoiding the treated area, indicating a strong repellent effect of the insecticide. The respiration rate of <em>A. gemmatalis</em> caterpillars decreased after exposure to indoxacarb. There was a reduction in food consumption by caterpillars treated with the insecticide, indicating a phagoinhibitory effect. In addition, histological changes were observed in the midgut of <em>A. gemmatalis</em>, including cytoplasmic vacuolization, an increased amount of cell fragments with nuclei released into the gut lumen, and degradation of the peritrophic matrix, indicating severe effects of cell degeneration. Indoxacarb caused both lethal and sublethal effects on <em>A</em>. <em>gemmatalis</em>, indicating its potential for the control of this pest.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"194 ","pages":"Article 107214"},"PeriodicalIF":2.5,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vertebrate frugivory in blueberry crops: Patterns across birds and mammals and consequences for yield 蓝莓作物中脊椎动物的果食:鸟类和哺乳动物的模式及其对产量的影响
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-22 DOI: 10.1016/j.cropro.2025.107213
José Javier Jiménez-Albarral , Daniel García , Marcos Miñarro
{"title":"Vertebrate frugivory in blueberry crops: Patterns across birds and mammals and consequences for yield","authors":"José Javier Jiménez-Albarral ,&nbsp;Daniel García ,&nbsp;Marcos Miñarro","doi":"10.1016/j.cropro.2025.107213","DOIUrl":"10.1016/j.cropro.2025.107213","url":null,"abstract":"<div><div>Damage and consumption of crops by vertebrates represent a significant source of conflict between humans and wildlife. This is particularly relevant in the context of small-fruit crops, such as blueberries, which are highly conspicuous, small-sized, and situated close to the ground, allowing a wide range of vertebrate species to swallow them whole and in large quantities. In the present study, we assessed the patterns of blueberry consumption by birds and mammals, and the effect of consumption on crop yield, in blueberry orchards in northern Spain. To this end, we characterised the fruit-eating vertebrate community through direct observation and camera traps in blueberry orchards, and conducted exclusion experiments in blueberry cultivars that ripen at different dates. The study was conducted in eight orchards over two consecutive years. We recorded 14 bird species and four mammal species consuming blueberries, with common blackbird (<em>Turdus merula</em> L.) and wild boar (<em>Sus scrofa</em> L.) accounting for more than 80 % of consumption events. Whereas frugivory by blackbirds was observed across orchards and cultivars, blueberry foraging by wild boar was highly localised. The exclusion experiments evidenced a negative impact of vertebrate frugivory on blueberry production. However, this impact was not homogeneous across sites, ranging from no impact to 74 % of yield loss. Similarly, vertebrate impact depended on the cultivar, with the early ripening cultivar being more susceptible to yield losses than late ripening cultivars. Further research is needed to ascertain what extrinsic (orchard and landscape structure) and intrinsic (abundance and physiological requirements of vertebrates) factors are responsible for the observed variability in yield loss across sites and cultivars. In terms of management, we recommend to prioritise bird deterrent methods to minimise the negative impact of vertebrates.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107213"},"PeriodicalIF":2.5,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Probing behavior analysis of foxglove aphids on soybean genotypes using the DC-EPG system 利用DC-EPG系统分析毛地黄蚜虫对大豆基因型的探测行为
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-21 DOI: 10.1016/j.cropro.2025.107210
Samuel A. Fasusi , Bo Yoon Seo , Hong Min Koh , Ji-Min Kim , Sungtaeg Kang
{"title":"Probing behavior analysis of foxglove aphids on soybean genotypes using the DC-EPG system","authors":"Samuel A. Fasusi ,&nbsp;Bo Yoon Seo ,&nbsp;Hong Min Koh ,&nbsp;Ji-Min Kim ,&nbsp;Sungtaeg Kang","doi":"10.1016/j.cropro.2025.107210","DOIUrl":"10.1016/j.cropro.2025.107210","url":null,"abstract":"<div><div>The foxglove aphid (FA), <em>Aulacorthum solani,</em> is a soybean pest that threatens food security. However, few studies on soybean plant resistance to FAs with resistance to <em>Aulacorthum solani</em> (<em>Raso</em>) genes have been conducted in Korea and Japan. Understanding how FAs infiltrate soybean plant tissues and identifying the location of resistance are crucial for the effective management of insect pests. This study focused on evaluating the probing behavior of FAs that fed on three susceptible and five (moderately and highly) resistant soybean genotypes using the electrical penetration graph (EPG) technique. We used EPG to visualize the movement of the aphid stylet and probing behavior in soybean tissues. We carefully studied the eight distinct waveforms generated during 14 h of feeding. The susceptible genotypes exhibited shorter duration and frequency of non-probing (np) waveforms but higher duration and frequency of E2 waveforms. In contrast, moderately and highly resistant genotypes produced fewer E2 waveforms but more np waveforms. The lower frequency and duration of the E2 waveform values from the resistant genotypes indicated that the resistance factor was located in the phloem. Therefore, phloem defense mechanisms inhibit FA activity. This pioneering study characterized the probing behavior of FAs in various soybean genotypes. Understanding the variations in FA probing behavior across different genotypes is useful for elucidating the mechanism of FA resistance and can guide future research on plant-insect interactions to assist in selecting resistant genotypes.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107210"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Diaporthe cinerascens from latent and symptomatic fig tree cankers using species-specific primers 利用种特异性引物检测无花果树潜伏性和症状性溃疡病中的孢子虫
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-21 DOI: 10.1016/j.cropro.2025.107211
Zeinab Bolboli, Hamed Negahban, Reza Mostowfizadeh-Ghalamfarsa
{"title":"Detection of Diaporthe cinerascens from latent and symptomatic fig tree cankers using species-specific primers","authors":"Zeinab Bolboli,&nbsp;Hamed Negahban,&nbsp;Reza Mostowfizadeh-Ghalamfarsa","doi":"10.1016/j.cropro.2025.107211","DOIUrl":"10.1016/j.cropro.2025.107211","url":null,"abstract":"<div><div>Fig canker is a multi-agent and complex disease, and since its identification, Diaporthe canker, primarily caused by <em>Diaporthe cinerascens</em>, has emerged as one of the most persistent and destructive diseases affecting fig trees in regions such as Iran, California, Canada, and Italy. The economic implications of this disease are considerable, further aggravated by the lack of effective therapeutic interventions. This study presents the development and validation of novel species-specific PCR primers designed to enhance the early and accurate detection of Diaporthe canker in artificially and naturally infected samples with mixed and latent infections. We utilized pure cultures of well-characterized <em>D. cinerascens</em> isolates, whose proven pathogenicity and genetic homogeneity were validated in earlier studies. Among the 82 developed primers, the TUB-Dc1 and HIS-Dc1 pairs were chosen after a thorough analysis of the β-tubulin (<em>tub</em>) and histone 3 (<em>his3</em>) genes, along with the internal transcribed spacer of rDNA (ITS) region. These selected primers successfully amplified target gene sequences in pure cultures and infected tissue samples using direct and nested PCR. The diagnostic sensitivity thresholds achieved were 100 fg μL<sup>−1</sup> for direct PCR and 1 ag μL<sup>−1</sup> for nested PCR. Notably, nested PCR significantly enhanced detection capabilities by up to 100,000-fold compared to direct PCR. The TUB-Dc1 primer pair effectively identified <em>D. cinerascens</em> in 80 % of samples with Type A canker, 22.7 % with Type C canker, and 8.8 % showing dieback symptoms. In comparison, conventional cultural methods detected the pathogen in only 30 % of Type A canker samples, and 4.4 % of Type C canker samples, with no detections in samples showing dieback. Furthermore, our results underscore the potential utility of our molecular tools for precise identification and detecting latent infections in asymptomatic samples via nested PCR. Our findings indicate that the species-specific assays are highly sensitive, specific, and reliable for detecting <em>D. cinerascens</em> in symptomatic and asymptomatic environmental samples. Additionally, this tool paves the way for future applications aimed at certifying pathogen-free fig saplings, an essential component for sustainable fig cultivation practices.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107211"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential parasitism performance and host adaptability of key biocontrol Eretmocerus species against two major whitefly pests 主要生物防治品种白蝇对两种主要粉虱的不同寄生性能及寄主适应性
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-21 DOI: 10.1016/j.cropro.2025.107212
Xi-Ya Wang , Jia-Wei Sun , Lucie S. Monticelli , Nicolas Desneux , Lian-Sheng Zang
{"title":"Differential parasitism performance and host adaptability of key biocontrol Eretmocerus species against two major whitefly pests","authors":"Xi-Ya Wang ,&nbsp;Jia-Wei Sun ,&nbsp;Lucie S. Monticelli ,&nbsp;Nicolas Desneux ,&nbsp;Lian-Sheng Zang","doi":"10.1016/j.cropro.2025.107212","DOIUrl":"10.1016/j.cropro.2025.107212","url":null,"abstract":"<div><div>Whiteflies are major agricultural pests, and their sustainable management depends on effective biological control strategies. This study investigates the preference, the host performance and the host feeding behavior of two key parasitoid species, <em>Eretmocerus corni</em> and <em>Eretmocerus hayati</em>, against <em>Bemisia tabaci</em> and <em>Trialeurodes vaporariorum</em>. To assess host selection, parasitism, host-feeding, emergence rates, developmental times, proportion of female offspring, and total mortality rate across different host species and instars, both no-choice and choice experiments were conducted. Key findings include: (1) <em>E. corni</em> successfully parasitized all instars of both whitefly species, while <em>E. hayati</em> completed its development only in <em>B. tabaci</em>. Both parasitoids achieved the highest parasitism rates and emergence rates on the second and third instars of their respective hosts. (2) <em>E. hayati</em> exhibited superior host-feeding, faster development, and higher total mortality rate compared to <em>E. corni</em>, additionally, both host-feeding rates and developmental time significantly decreased as the host instar increased. (3) Although <em>E. hayati</em> could not complete its development in <em>T. vaporariorum</em>, it caused substantial mortality through aborted parasitism and superior host-feeding. (4) In the choice tests, <em>E. corni</em> showed a significant preference for <em>T. vaporariorum</em> when both whitefly species were at the same instar stage, whereas <em>E. hayati</em> exhibited no notable host preference. (5) Under the mixed-host condition, both <em>E. corni</em> and <em>E. hayati</em> exhibited increased mortality rates against whiteflies at the same instar compared to the single-host condition. These findings offer valuable insights into the host preference, performance of these parasitoids and provide theoretical guidance for optimizing whitefly biological control.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107212"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Herbicide selectivity and efficacy in weed control in canola production system 油菜籽生产系统除草剂防治杂草的选择性和有效性研究
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-20 DOI: 10.1016/j.cropro.2025.107207
Natalia Costa , Guilherme Vieira Pimentel , Amanda Santana Chales , Luiz Daniel Rodrigues da Silva , Elisabete da Cruz Silva Watanabe , Adenilson Henrique Gonçalves
{"title":"Herbicide selectivity and efficacy in weed control in canola production system","authors":"Natalia Costa ,&nbsp;Guilherme Vieira Pimentel ,&nbsp;Amanda Santana Chales ,&nbsp;Luiz Daniel Rodrigues da Silva ,&nbsp;Elisabete da Cruz Silva Watanabe ,&nbsp;Adenilson Henrique Gonçalves","doi":"10.1016/j.cropro.2025.107207","DOIUrl":"10.1016/j.cropro.2025.107207","url":null,"abstract":"<div><div>Canola is an essential oilseed in global agriculture, contributing to the economic, social, and environmental sectors due to its versatility and nutritional value. Despite the notable growth of canola in Brazil, weed management poses a challenge due to the scarcity of registered selective herbicides and the prohibition of transgenics, resulting in productivity losses. Therefore, it is crucial to develop innovative technologies and generate knowledge to guide effective weed management and facilitate the expansion of canola in the country. Field studies during the 2022/2023 growing season evaluated the selectivity of herbicides in canola production and their effectiveness in weed control. The pre-emergent treatments were: clomazone, s-metolachlor, clomazone + s-metolachlor, and no pre-emergent herbicide; and the post-emergent treatments were: quinclorac, dicamba, quinclorac + dicamba, and no post-emergent herbicide, in addition to a weed-free control. Although clomazone, quinc lorac, and dicamba are not registered for use in canola in Brazil, they showed promising results by significantly reducing weed interference without negatively impacting oil and protein contents or yield components. Combinations S-metolachlor + clomazone and quinclorac + dicamba, provided the best outcomes in both weed control and grain productivity, with yields reaching 2053.29 kg ha<sup>−1</sup> in Lavras. These findings suggest that the tested herbicides have potential for selective use in canola production, paving the way for further studies aimed at their registration and incorporation into production systems.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107207"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resistance of ten Indonesian cassava cultivars to the cassava lacebug, Vatiga illudens (Drake) (Hemiptera: Tingidae), a newly invasive exotic species in Asia 印度尼西亚10个木薯品种对亚洲新入侵外来种木薯粉虱(半翅目:粉虱科)的抗性
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-19 DOI: 10.1016/j.cropro.2025.107208
Retno Dyah Puspitarini, Ave Maria Simbolon, Chairunisah Putri, Hendy Dwi Bayu Ardianto, Vriescha Ermalitha Ramadhanti Rudiantoyo, Windi Puspa Saputri, Faiz Nashiruddin Muhammad, Ito Fernando
{"title":"Resistance of ten Indonesian cassava cultivars to the cassava lacebug, Vatiga illudens (Drake) (Hemiptera: Tingidae), a newly invasive exotic species in Asia","authors":"Retno Dyah Puspitarini,&nbsp;Ave Maria Simbolon,&nbsp;Chairunisah Putri,&nbsp;Hendy Dwi Bayu Ardianto,&nbsp;Vriescha Ermalitha Ramadhanti Rudiantoyo,&nbsp;Windi Puspa Saputri,&nbsp;Faiz Nashiruddin Muhammad,&nbsp;Ito Fernando","doi":"10.1016/j.cropro.2025.107208","DOIUrl":"10.1016/j.cropro.2025.107208","url":null,"abstract":"<div><div>The cassava lacebug, <em>Vatiga illudens</em> (Drake) (Hemiptera: Tingidae), is a pest that feeds on cassava foliage, significantly reducing the yield of tuberous roots. Recently introduced to Asia, this invasive exotic pest has been reported only in Indonesia, where it poses a serious threat to cassava cultivation. This study aimed to determine the resistance levels of ten Indonesian cassava cultivars to <em>V. illudens</em>. The leaf nutritional profile (proximate content), as well as phenol and hydrogen cyanide (HCN) content, were analyzed as potential factors affecting the biological parameters of <em>V. illudens</em>. Results from the choice test revealed that <em>V. illudens</em> adults exhibited a clear preference for certain cultivars, with the highest numbers observed on Gajah (10.00 individuals), Adira 1 (5.60 individuals), and Mentega (5.20 individuals). In contrast, adult numbers on the remaining cultivars were lower, ranging from 2.40 to 3.60 individuals. In the no-choice antibiosis test, the nymphal developmental period (from first instar to adult) ranged from 11.70 to 20.40 days, while adult longevity varied from 9.05 to 16.30 days. Leaf HCN content was the only chemical property found to be associated with the degree of resistance in cassava cultivars, with low-HCN cultivars like Gajah (9.66 mg/g) and Mentega (11.26 mg/g) being more preferred by <em>V. illudens</em>. The insect also exhibited faster development and extended adult longevity on these cultivars, leading to increased population growth. These findings are expected to provide valuable information for the management of <em>V. illudens</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107208"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro and in vivo compatibility of isocycloseram-based insecticides with entomopathogenic fungi used for corn leafhopper management 异环菌胺类杀虫剂与玉米叶蝉昆虫病原真菌的体内外相容性研究
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-17 DOI: 10.1016/j.cropro.2025.107204
Maria Eduarda Sperotto , Matheus Rakes , Maíra Chagas Morais , Daniel Bernardi , Leandro do Prado Ribeiro
{"title":"In vitro and in vivo compatibility of isocycloseram-based insecticides with entomopathogenic fungi used for corn leafhopper management","authors":"Maria Eduarda Sperotto ,&nbsp;Matheus Rakes ,&nbsp;Maíra Chagas Morais ,&nbsp;Daniel Bernardi ,&nbsp;Leandro do Prado Ribeiro","doi":"10.1016/j.cropro.2025.107204","DOIUrl":"10.1016/j.cropro.2025.107204","url":null,"abstract":"<div><div>Recently registered, isocycloseram-based insecticides have the corn leafhopper (<em>Dalbulus maidis</em>) as a main target in Brazil. Nevertheless, the management of this vector is complex and requires the integration of strategies, including chemical and fungal agents. Here, we investigated the compatibility of mycoinsecticides based on isolate Esalq-1296 of <em>Cordyceps javanica</em> and Simbi BB15 of <em>Beauveria bassiana</em> with isocycloseram-based insecticides and their mixtures. <em>In vitro</em>, the biological index adopted indicated that the insecticide based on isocycloseram + lambda-cyhalothrin (at 125- and 250-mL ha<sup>−1</sup>) was classified as moderately compatible with the Esalq-1296 isolate of <em>C. javanica</em> and Simbi BB15 of <em>B. bassiana</em>. The isocycloseram-based formulation was classified as compatible with the Simbi BB15 isolate of <em>B. bassiana</em>, at both doses tested, and moderate compatible and compatible with the Esalq-1296 isolate of <em>C. javanica</em> at 100 and 50 mL ha<sup>−1</sup>, respectively. Further analysis revealed that the majority of insecticide-mycoinsecticides mixtures exhibited physicochemical compatibility, with the exception of the mixture comprising these insecticides and the mycoinsecticide-based on <em>B. bassiana</em> (FlyControl®), which demonstrated an absence of homogeneity. The <em>in vivo</em> bioassays demonstrated an additive effect on leafhopper mortality and an increase in the velocity of lethal toxicity when these insecticides (at LC<sub>30</sub>) were combined with the tested mycoinsecticides. However, the insecticides reduced the extrusion rate and sporulation from dead leafhoppers. As these parameters are crucial for fungal persistence by establishing a secondary cycle of epizootics in cornfields, caution is advised when combining these chemical and fungal agents for the management of <em>D. maidis</em>.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"194 ","pages":"Article 107204"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum “Cyantraniliprole-induced intergenerational sublethal effects on fall armyworm, Spodoptera frugiperda” [Crop Protect. 190 107116 https://doi.org/10.1016/j.cropro.2025.107116] 更正:"氰虫酰胺诱导的对秋季军虫(Spodoptera frugiperda)的代际亚致死效应" [Crop Protect. 190 107116 https://doi.org/10.1016/j.cropro.2025.107116]
IF 2.5 2区 农林科学
Crop Protection Pub Date : 2025-03-17 DOI: 10.1016/j.cropro.2025.107199
Arzlan Abbas , Ali Hasnain , Faisal Hafeez , Wu Hai Chao , Dong Yu Hua , Aleena Alam , Sohail Abbas , Ayesha Iftikhar , Hamed A. Ghramh , Khalid Ali Khan , Farman Ullah , Chen Ri Zhao
{"title":"Corrigendum “Cyantraniliprole-induced intergenerational sublethal effects on fall armyworm, Spodoptera frugiperda” [Crop Protect. 190 107116 https://doi.org/10.1016/j.cropro.2025.107116]","authors":"Arzlan Abbas ,&nbsp;Ali Hasnain ,&nbsp;Faisal Hafeez ,&nbsp;Wu Hai Chao ,&nbsp;Dong Yu Hua ,&nbsp;Aleena Alam ,&nbsp;Sohail Abbas ,&nbsp;Ayesha Iftikhar ,&nbsp;Hamed A. Ghramh ,&nbsp;Khalid Ali Khan ,&nbsp;Farman Ullah ,&nbsp;Chen Ri Zhao","doi":"10.1016/j.cropro.2025.107199","DOIUrl":"10.1016/j.cropro.2025.107199","url":null,"abstract":"","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107199"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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