Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献

{"title":"The effects of expanded polystyrene particle on energy metabolism of the sea slater (Ligia cinerascens) originating from a highly EPS-polluted area","authors":"Youn-Ha Lee ,&nbsp;Je-WonYoo ,&nbsp;Jee-Hyun Jung ,&nbsp;Young-Mi Lee","doi":"10.1016/j.cbpc.2024.110118","DOIUrl":"10.1016/j.cbpc.2024.110118","url":null,"abstract":"<div><div>Due to its high concentration and persistence, microplastic (MP) pollution is a major threat to marine environments. Expanded polystyrene (EPS) particles are the most abundant MP type in Asian regions, including the Korean coastal region. Although many previous studies have reported the toxicity of MPs to marine biota, the toxicity of environmentally relevant MPs to coastal organisms is not well understood. Thus, we investigated the toxicity of EPS on the growth and energy metabolism of the juvenile marine isopods, <em>Ligia cinerascens</em>, obtained from a population that has been exposed to EPS through multiple generations. After 14 and 21 days of dietary EPS exposure, body weight and molting of <em>L. cinerascens</em> were unaffected. However, the energy reserves (carbohydrates, proteins, and lipids) were significantly reduced, resulting in a decrease in the total energy budget (E_a) by dietary EPS exposure. The transcriptional modulation patterns of genes related to energy metabolism suggested that dietary EPS exposure may increase the digestion of non-carbohydrate sources, such as proteins and lipids, to compensate for increased energy expenditure. Our findings suggest that dietary EPS exposure, although no toxic at the individual level, can reduce the energy status of juvenile marine isopods, which provides useful information on the toxic effects of environmentally relevant MPs to coastal ecosystem.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110118"},"PeriodicalIF":3.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity and immunomodulation of four novel cathelicidin genes isolated from the tiger frog Hoplobatrachus rugulosus","authors":"Danni Huang ,&nbsp;Fulong Gao ,&nbsp;Yixin Huang ,&nbsp;Ronghui Zheng ,&nbsp;Chao Fang ,&nbsp;Wenshu Huang ,&nbsp;Kejian Wang ,&nbsp;Jun Bo","doi":"10.1016/j.cbpc.2024.110091","DOIUrl":"10.1016/j.cbpc.2024.110091","url":null,"abstract":"<div><div>Cathelicidin is a family of antimicrobial peptides in vertebrates that plays an important role in resistance and immunization against pathogenic microorganisms. In the present study, the full-length cDNA sequences of four novel cathelicidins (<em>cathelicidin-1</em> to <em>cathelicidin-4</em>) in the tiger frog <em>Hoplobatrachus rugulosus</em>, encoding 153, 188, 132, and 160 amino acids, respectively, were firstly cloned by rapid amplification of the cDNA ends (RACE) technique. Sequence comparison and phylogenetic tree analysis indicated that the structures of the four cathelicidins are highly diverse. Afterwards, the tissue distribution profiles and antimicrobial patterns of cathelicidins in <em>H. rugulosus</em> were determined by real-time PCR. The four cathelicidins showed tissue-specific distribution patterns in the healthy frogs, and the transcriptional levels of cathelicidins exhibited a tissue- and time-dependency profile in the frogs challenged with pathogenic bacteria <em>Aeromonas hydrophila</em> for 72 h. The synthetic peptides of <em>cathelicidin-1</em> and <em>cathelicidin-2</em> exhibited broad-spectrum <em>in vitro</em> antimicrobial activity, and cathelicidins exerted antimicrobial activities through excessive induction of reactive oxygen species and direct disruption of the microbial membrane structure. In addition, the intraperitoneal injection of cathelicidin proteins significantly increased the marine medaka <em>Oryzias melastigma</em> resistance to bacterial challenges. The existence of multiple cathelicidins, their distinct tissue distribution patterns, and the inducible expression profiles suggest a sophisticated, highly redundant, and multilevel network of antimicrobial defense mechanisms in tiger frogs. This study provides evidence that cathelicidins have antimicrobial and immunomodulatory activities, and cathelicidins derived from <em>H. rugulosus</em> have potential therapeutic applications against pathogenic infections in aquaculture.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110091"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions of a PFOS/sodium nitrite mixture in Chinese mitten crab (Eriocheir sinensis): Impacts on survival, growth, behavior, energy metabolism and hepatopancreas transcriptome","authors":"Qiuran Yu ,&nbsp;Song Wang ,&nbsp;Erchao Li ,&nbsp;Yiwen Yang ,&nbsp;Xiaodan Wang ,&nbsp;Chuanjie Qin ,&nbsp;Jian G. Qin ,&nbsp;Liqiao Chen","doi":"10.1016/j.cbpc.2024.110114","DOIUrl":"10.1016/j.cbpc.2024.110114","url":null,"abstract":"<div><div>Perfluorooctanesulfonic acid (PFOS) and sodium nitrite may have complex adverse effects on aquatic animals. This study assessed the interactive effects of PFOS and sodium nitrite on Chinese mitten crab (<em>Eriocheir sinensis</em>). A 2 × 3 factorial experiment with 0, 0.1, and 5 mg/L PFOS and 0, 3.50 mg/L sodium nitrite evaluated impacts on growth, behavior, oxygen consumption, health, energy metabolism, and hepatopancreas transcriptome. PFOS &lt;0.1 mg/L with 3.50 mg/L nitrite significantly decreased PFOS accumulation in the hepatopancreas and improved feeding rates and hepatopancreas structure (<em>P</em> &lt; 0.05). Under 5 mg/L PFOS and nitrite, survival, weight gain, hepatosomatic index, and feeding rate significantly decreased (<em>P</em> &lt; 0.05). PFOS (0.1 mg/L) with nitrite significantly prolonged righting response time and increased locomotion speed (<em>P</em> &lt; 0.05). PFOS (5 mg/L) significantly decreased serum triglyceride and lactate levels, while PFOS and nitrite decreased glucose, triglyceride, and glycogen levels and increased lactate in hepatopancreas (<em>P</em> &lt; 0.05). Transcriptomic analysis indicated that PFOS affects p53 signaling, cell cycle and neurotransmission pathways, with notable changes in cell proliferation genes (<em>pcna</em>, <em>ccna</em>, <em>cdk1</em>, <em>cdk2</em>, <em>rbx1</em>) primarily downregulated by PFOS. Overall, PFOS disrupts neurotransmitter regulation and causes hepatopancreatic damage, while nitrite can reduce the toxicity of PFOS by decreasing its hepatopancreas accumulation. However, high levels of PFOS combined with sodium nitrite exacerbate toxicity, emphasizing the need for comprehensive assessment of environmental pollutant interactions.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110114"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of cyantraniliprole sublethal doses on the population dynamics and gene expression of Aphis gossypii Glover (Hemiptera: Aphididae)","authors":"Yuepeng Qiu ,&nbsp;Xianpeng Song ,&nbsp;Dan Wang ,&nbsp;Hongyan Hu ,&nbsp;Changcai Wu ,&nbsp;Yongpan Shan ,&nbsp;Yajie Ma ,&nbsp;Xiangliang Ren ,&nbsp;Yan Ma","doi":"10.1016/j.cbpc.2024.110113","DOIUrl":"10.1016/j.cbpc.2024.110113","url":null,"abstract":"<div><div>Cyantraniliprole (CYA), widely recognized as a highly effective solution, is widely used in pest management. It has been broadly utilized to manage diverse pests, among which <em>Aphis gossypii</em> Glover (Hemiptera: Aphididae) is a prominent agricultural pest that leads to significant crop damage worldwide. Studies suggest that the sublethal effect of insecticides might contribute to the resurgence of <em>A. gossypii</em>. Therefore, in this study, <em>A. gossypii</em> were exposed to sublethal doses of CYA (LC₁₅ and LC₃₀ values of 1.43 and 2.93 mg/L, respectively) for 48 h then employed life table parameters and RT-qPCR were used to estimate the sublethal and cross-generational impacts. Treatments with sublethal doses of CYA notably reduced the survival and reproduction of the F₀ <em>A. gossypii</em> and CYA at LC₃₀ significantly increased the fecundity and population growth parameters (<em>R</em>₀, <em>r</em>, <em>λ</em>, and <em>GRR</em>) of F₁ and reduced in the pre-adult stage. Furthermore, gene expression analysis indicated a significant downregulation of juvenile hormone-binding protein (<em>JHBP</em>) in F₀. Conversely, the F₁ generation exhibited an upregulation of vitellogenin (<em>Vg</em>), insulin receptor substrate 1 (<em>InS1</em>), ecdysone receptor (<em>EcR</em>), and ultraspiracle protein (<em>USP</em>). The funding not only enhance the comprehension of the sublethal effects of CYA on <em>A. gossypii</em> but also provide valuable guidance for the effective utilization of insecticides in managing the pest.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110113"},"PeriodicalIF":3.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zearalenone delays tissue regeneration by dysregulating neutrophil balance in zebrafish (Danio rerio) larvae","authors":"Rui Luo ,&nbsp;Bo Wang ,&nbsp;Rui Luo","doi":"10.1016/j.cbpc.2024.110105","DOIUrl":"10.1016/j.cbpc.2024.110105","url":null,"abstract":"<div><div>Zearalenone (ZEA), a common mycotoxin, poses significant environmental and health risks. While its toxicological effects are well-studied, its impact on regeneration remains unclear. This study explored ZEA's effects on zebrafish (<em>Danio rerio</em>) larvae, focusing on developmental toxicity, immunotoxicity, and tissue regeneration. Embryos were exposed to 0, 0.5, 1, and 1.5 μM ZEA from 6 to 72 h post-fertilization (hpf). Although hatching and survival rates remained unaffected, malformations, including body axis bending and pericardial edema, increased dose-dependently, with 4.44 % abnormalities observed at 1.5 μM (<em>p</em> = 0.01). Heart rates also declined significantly at 1.5 μM (75.40 vs. 72.53 beats/30s, <em>p</em> = 0.0054). Immunotoxicity was assessed using Tg(mpx: eGFP) zebrafish to monitor neutrophil responses post-injury. ZEA exposure led to increased neutrophil counts (229.87 vs. 330.80, <em>p</em> &lt; 0.0001) and chemotaxis (21.15 % vs. 34.57 %, p &lt; 0.0001). RNA sequencing of 0 and 1.5 μM groups revealed disrupted redox balance and oxygen transport, with down-regulation of hbae1, hbbe2, and hbae3 and up-regulation of hif1a, indicating hypoxia involvement. Elevated reactive oxygen species (ROS), reduced antioxidant enzyme activity, and increased apoptosis were also observed.</div><div>Tail fin regeneration assays showed delayed regeneration at 1 and 1.5 μM ZEA, linked to impaired immune function and oxidative stress. These findings highlight ZEA's adverse effects on developmental and regenerative processes, underscoring its environmental and health implications and the need for further research.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110105"},"PeriodicalIF":3.9,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic chlorpyrifos exposure induces oxidative stress, neurological damage, and hepatopancreas enrichment in Chinese mitten crab (Eriocheir sinensis)","authors":"Yuanyuan Tan ,&nbsp;Jiaming Dong ,&nbsp;Luyao Wang ,&nbsp;Weining Li ,&nbsp;Jie Bao ,&nbsp;Hongbo Jiang","doi":"10.1016/j.cbpc.2024.110111","DOIUrl":"10.1016/j.cbpc.2024.110111","url":null,"abstract":"<div><div>The toxic effects of long-term exposure to low doses of chlorpyrifos (CPF) on <em>Eriocheir sinensis</em> were evaluated using acute toxicity tests, transcriptome analyses, and metabolome profiling. Four groups (three replicates per group, 60 crabs)—control (no CPF exposure), high exposure (0.12 mg/L CPF), medium exposure (0.036 mg/L), and low exposure (0.012 mg/L)—were subjected to CPF for 21 days. Tissue damage, antioxidant enzyme activity, transcriptional changes, and metabolic alterations in <em>E. sinensis</em> were analyzed. The results demonstrated that CPF disrupted the regulatory networks of transcription and metabolism in crabs under the experimental concentration conditions, with the severity of effects increasing as the duration of exposure lengthened despite the crabs' efforts to activate key defense mechanisms, such as upregulation of cholinesterase 1-like gene expression, to counteract organophosphorus toxicity and adapt to CPF presence in their environment. Even at low concentrations (0.012 mg/L), neurobehavioral development and the antioxidant kinase system in crabs were impaired, leading to hepatopancreatic tissue lesions that negatively affect their growth and survival rates. Additionally, <em>E. sinensis</em> accumulates significant levels of CPF, which may pose food safety concerns when humans consume them. Therefore, ensuring the rational use of CPF requires maintaining appropriate water concentrations to minimize direct harm to aquatic organisms and indirect impacts on food safety associated with this pesticide.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110111"},"PeriodicalIF":3.9,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-acute exposure of sea urchin (Strongylocentrotus intermedius) to environmentally relevant concentrations of PFOA and GenX influences gonadal development","authors":"Hongce Song ,&nbsp;Meiyun Dong ,&nbsp;Weizhong Wang ,&nbsp;Xiaona Wang ,&nbsp;Deyang Tian ,&nbsp;Xiaotong Wang ,&nbsp;Dong Xu","doi":"10.1016/j.cbpc.2024.110104","DOIUrl":"10.1016/j.cbpc.2024.110104","url":null,"abstract":"<div><div>Perfluorooctanoic acid (PFOA) and its substitute, hexafluoropropylene oxide dimer acid (GenX), are widely used perfluorinated compounds (PFCs) that pose significant risks to marine ecosystems. However, the specific impacts of these contaminants on marine invertebrates, particularly echinoderms, remain poorly understood. <em>Strongylocentrotus intermedius</em>, a globally significant benthic aquacultural species, may be potentially affected by PFCs. This study aimed to assess the reproductive toxicity of PFOA and GenX in <em>S. intermedius</em>. After exposing <em>S. intermedius</em> to either PFOA or GenX for 7 or 14 days, it was observed that even at environmentally relevant concentrations (2 μg/L), both compounds inhibited normal growth and gonadal development in <em>S. intermedius</em>, with effects becoming more pronounced over time. Further analysis revealed that prolonged exposure to PFCs resulted in a significant reduction in energy reserves (glycogen, lipids, and proteins) and caused abnormal changes in metabolic enzyme activities, with PFOA exhibiting more pronounced effects compared to GenX. At the genetic level, the expression of genes related to gonadal development initially increased and then decreased as the concentrations of the compounds rose. Additionally, integrated biomarker response analysis indicated that PFOA had greater reproductive toxicity than GenX, in terms of both concentration and exposure duration. These results provided a preliminary evaluation of the impact of PFCs on marine invertebrates, offering a foundation for further research into their ecological risks and contributing to the development of more comprehensive environmental risk assessments for these contaminants.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110104"},"PeriodicalIF":3.9,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome-based analysis reveals the toxic effects of perfluorononanoic acid by affecting the development of the cardiovascular system and lipid metabolism in zebrafish","authors":"Mingqing Qian ,&nbsp;Weiqiang Sun ,&nbsp;Lin Cheng ,&nbsp;Yuanyuan Wu ,&nbsp;Li Wang ,&nbsp;Hui Liu","doi":"10.1016/j.cbpc.2024.110108","DOIUrl":"10.1016/j.cbpc.2024.110108","url":null,"abstract":"<div><div>Perfluorononanoic acid (PFNA) is a perfluoroalkyl acid containing nine carbon chains, with an additional carbon‑fluorine bond that makes it more stable and toxic. Studies have shown that PFNA can harm the reproductive, immune, and nervous systems, as well as many organs, which can increase the risk of cancer. In this study, zebrafish embryos were treated with 0 and 100 μM PFNA for 72 and 96 hpf, and their angiogenesis and haematopoiesis were observed under laser confocal microscopy using <em>Tg (fli1:EGFP)</em> and <em>Tg (gata1:DsRed)</em> transgenic zebrafish. The data showed that PFNA exposure decreased heart rate and slowed blood flow in zebrafish. PFNA was found to inhibit erythropoiesis by O-dianisidine staining. RNA-seq analysis was used to compare gene expression changes in zebrafish from control and 100 μM PFNA-exposed groups at 72 hpf. KEGG results showed significant enrichment of PPAR signaling pathway, fatty acid metabolism, steroid biosynthesis and apoptosis. The RNA-seq results were validated by real-time fluorescence quantitative PCR (RT-qPCR). Oil red O staining and Filipin staining showed increased lipid accumulation after PFNA exposure, and TUNEL staining showed that PFNA exposure led to apoptosis. In conclusion, exposure to PFNA may cause toxic effects in zebrafish by affecting cardiovascular development, causing lipid accumulation and promoting apoptosis.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110108"},"PeriodicalIF":3.9,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of an environmental ototoxic pollutant on fish fighting behaviors","authors":"Jiun-Lin Horng ,&nbsp;Yu-Huan Hu ,&nbsp;Hsi Chen ,&nbsp;Ming-Yi Chou","doi":"10.1016/j.cbpc.2024.110103","DOIUrl":"10.1016/j.cbpc.2024.110103","url":null,"abstract":"<div><div>Numerous environmental pollutants exhibit ototoxicity and cause damage to the lateral line structures in fish, including the neuromast and its hair cells. The lateral line is used to detect hydrodynamic changes and is thought to play a significant role in aggressive interactions. Fighting behaviors in fish are crucial for establishing social hierarchy and obtaining limited resources. In this study, we ablated the function of hair cells using a commonly used ototoxin, neomycin, to evaluate the impact of this ototoxic pollutant on fighting behavior through damaging the lateral line. Our results showed that the number of wins and the duration of dyadic fight behavior decreased in zebrafish with lateral line ablation. These zebrafish also exhibited increased anxiety and biting frequencies. On the other hand, social preferences and fitness were not affected in lateral line-ablated zebrafish. In conclusion, the lateral line mechanosensory system is crucial for fish to gather sufficient information and make correct decisions during conflicts and fighting behaviors. Impairment of hair cell function can affect aggressive behaviors and decision-making in fish, subtly altering their behavioral patterns and leading to significant impacts on the aquatic ecosystem.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110103"},"PeriodicalIF":3.9,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nanopolystyrene and/or phoxim exposure on digestive function of Eriocheir sinensis","authors":"Mengting Huang ,&nbsp;Yuan Ma ,&nbsp;Qianru Fan ,&nbsp;Shunli Che ,&nbsp;Jun Zhang ,&nbsp;Shuquan Ding ,&nbsp;Shuren Zhu ,&nbsp;Xilei Li","doi":"10.1016/j.cbpc.2024.110102","DOIUrl":"10.1016/j.cbpc.2024.110102","url":null,"abstract":"<div><div>Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab (<em>Eriocheir sinensis</em>), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of <em>Eriocheir sinensis</em> to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 10¹⁰ particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including <em>CHT</em>, <em>CarL</em>, and <em>CarB</em>, suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including <em>PPARγ</em>, <em>GYK</em>, <em>PEPCK</em>, and <em>SCD</em>, as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in <em>E. sinensis</em> by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of <em>Eriocheir sinensis</em>.</div></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"289 ","pages":"Article 110102"},"PeriodicalIF":3.9,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}