Clinics最新文献

{"title":"Personalized nutrition and precision medicine in perimenopausal women: A minireview of genetic polymorphisms COMT, FUT2, and MTHFR","authors":"Pedro Andrade ,&nbsp;Aline Boveto Santamarina ,&nbsp;Jéssica Alves de Freitas ,&nbsp;Annete Bressan Rente Ferreira Marum ,&nbsp;Ana Flávia Marçal Pessoa","doi":"10.1016/j.clinsp.2024.100549","DOIUrl":"10.1016/j.clinsp.2024.100549","url":null,"abstract":"<div><div>This mini-review explores the potential of precision medicine and personalized nutrition in addressing health challenges faced by perimenopausal women, focusing on the role of genetic polymorphisms in key metabolic pathways. Specifically focus on the single nucleotide polymorphisms (SNPs) in the COMT, FUT2, and MTHFR genes, which influence neurotransmitter metabolism, gut microbiota composition, and folate homeostasis, respectively. These polymorphisms are critical in modulating hormonal fluctuations, metabolic imbalances, and nutrient absorption during perimenopause. The review highlights the impact of COMT rs4680 on stress response and mood disorders, FUT2 rs602662 and rs601338 on vitamin B12 absorption and cortisol metabolism, and MTHFR rs1801133 and rs1801131 on homocysteine levels and cardiovascular risk. Furthermore, the integration of machine learning in precision medicine is discussed, offering insights into how genetic data can optimize personalized interventions. This approach enables targeted nutritional and therapeutic strategies to mitigate the metabolic and psychological effects of perimenopause. Overall, this review underscores the importance of incorporating genetic testing into preventive care for perimenopausal women to enhance quality of life and promote healthy aging.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100549"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Licochalcone A on proliferation, invasion, and drug resistance of glioma cells by regulating TLR4/NF-κB signaling pathway","authors":"Baigui Zhou ,&nbsp;Kun Mu ,&nbsp;Xuzhou Yu ,&nbsp;Xu Chen ,&nbsp;Xiaoying Shi","doi":"10.1016/j.clinsp.2024.100542","DOIUrl":"10.1016/j.clinsp.2024.100542","url":null,"abstract":"<div><h3>Objective</h3><div>Based on Toll Like Receptor 4 (TLR4)/Nuclear Factor-κB (NF-κB) Exploring the effects of Licochalcone A (LCA) on the proliferation, invasion, and drug resistance of glioma cells through signaling pathways.</div></div><div><h3>Methods</h3><div>Cultivate human glioma cell line U251 in vitro, induce drug-resistant cell line U251/TMZ with Temozolomide (TMZ), and validate the results. Different concentrations of licorice chalcone A were used to treat U251 cells and U251/TMZ cells, and were named as control group, low-dose group, medium-dose group, and high-dose group, respectively. CCK-8 assay, cell adhesion assay, and Transwell assay were used to detect cell survival rate, cell adhesion rate, number of migrating cells, and number of invading cells, respectively.</div></div><div><h3>Results</h3><div>The cell survival rate, cell adhesion rate, number of migrating and invading cells in the high-dose group were lower than those in the medium-dose group and lower than those in the control group. High-dose group TLR4, NF-κB mRNA and protein levels were lower than those in the medium dose group and lower than those in the control group (<em>p</em> &lt; 0.05). Compared with the si-NC group, the si-TLR4 group showed a decrease in cell survival rate and adhesion rate, as well as a decrease in the number of migrating and invading cells, the levels of CyclinD1 and N-cadherin proteins decreased, while the levels of E-cadherin protein increased (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>LCA could inhibit the proliferation and metastasis of glioma cells and reverse drug resistance, possibly by inhibiting the TLR4/NF-κB signaling pathway.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100542"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge and attitude of deaf women in relation to contraceptive methods: A systematic review","authors":"Gabriela Fuster Barbosa ,&nbsp;Edson Santos Ferreira-Filho ,&nbsp;Lais Abdo Quintão ,&nbsp;Laura Fernandes Berto ,&nbsp;Patrícia Gonçalves de Almeida ,&nbsp;Edmund Chada Baracat ,&nbsp;Luis Bahamondes ,&nbsp;José Maria Soares-Junior ,&nbsp;Isabel Cristina Esposito Sorpreso","doi":"10.1016/j.clinsp.2024.100558","DOIUrl":"10.1016/j.clinsp.2024.100558","url":null,"abstract":"<div><h3>Background</h3><div>Communication barriers make it difficult for deaf women to access information about contraceptive methods, making them vulnerable to unplanned pregnancies.</div></div><div><h3>Objective</h3><div>To identify knowledge and attitudes of deaf women in relation to contraceptive methods.</div></div><div><h3>Method</h3><div>The authors conducted a systematic review of the literature, PROSPERO registry (CRD42021277635), conducted from August 2021 to April 2024. The included studies focused on knowledge and attitudes about contraception among women with deafness.</div></div><div><h3>Results</h3><div>Twelve articles were included, with a total of 2,641 participants and those reported low knowledge about contraceptive methods. Nine studies showed withdrawal, condoms, and oral contraceptives as the best methods that deaf women know. Five studies showed low knowledge about intrauterine devices and subdermal implants. The main reasons given for abandoning the method were reproductive desire and fear of side effects. The use of hormonal contraceptives was higher among adult women and, two studies showed that among teenagers the practice of withdrawal was higher than the use of condoms. The barriers most mentioned in the eight articles for the use of contraceptives were those of communication.</div></div><div><h3>Conclusion</h3><div>Knowledge of contraceptives among deaf women was low; however, the attitude of acceptance of the use of contraceptive methods was favorable, despite the little access to more effective methods, especially among adolescents. The factor associated with low knowledge evidenced in the systematic review was communication barriers.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100558"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small fiber neuropathy with dysautonomia and positive GPCR and FGFR-3 antibodies after the first BNT162b2 dose","authors":"Josef Finsterer ,&nbsp;Carla Alexandra Scorza ,&nbsp;Fulvio Alexandre Scorza ,&nbsp;Ana C. Fiorini","doi":"10.1016/j.clinsp.2024.100557","DOIUrl":"10.1016/j.clinsp.2024.100557","url":null,"abstract":"","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100557"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of dry eye in Brazil: Home survey reveals differences in urban and rural regions","authors":"Leidiane Adriano Pereira,&nbsp;Laura Braga Arantes,&nbsp;Etiene Lorriane de Souza Persona,&nbsp;Denny Marcos Garcia,&nbsp;Isvander Gustavo de Souza Persona,&nbsp;Regina Celia Nucci Pontelli,&nbsp;Eduardo M. Rocha","doi":"10.1016/j.clinsp.2025.100578","DOIUrl":"10.1016/j.clinsp.2025.100578","url":null,"abstract":"<div><h3>Purpose</h3><div>A random home visit search investigated the prevalence and predictors of Dry Eye (DE) symptoms in Brazil.</div></div><div><h3>Methods</h3><div>Through home visits to 420 urban and 180 rural residences in the countryside of Southeast Brazil, the Dry Eye Disease Short Questionnaire (DEDSQ) was applied to volunteers 40 years and older. The predictive value of the DEDSQ and the risk factors were investigated.</div></div><div><h3>Results</h3><div>DE is more frequent in the urban population (38.1 % vs. 20 %; <em>p</em> &lt; 0.0001) and was higher in females (37.5 % and 21.5 %; <em>p</em> &lt; 0.0001). In the urban area, DE is associated with dyslipidemia (<em>p</em> &lt; 0.0001), visual display terminal use &gt; 2 h/day (<em>p</em> &lt; 0.0001), and pterygium (<em>p</em> &lt; 0.001), and in the rural area with dyslipidemia (<em>p</em> &lt; 0.01) and prior ocular surgery (<em>p</em> &lt; 0.05). In women, the predictors are antiallergics, chronic pelvic pain, fibromyalgia, and visual display terminal use &gt; 2h/day. Aging was associated with distinct risk factors. DEDSQ showed 78.5 % of sensitivity and 71.1 % specificity.</div></div><div><h3>Conclusion</h3><div>This first domiciliary study in Brazil reveals that DE symptoms were more prevalent in the urban area and among women. DE has distinct risk factors and a high prevalence in Brazil.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100578"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plants used by Brazilian communities of African descent for women's health","authors":"Letícia Francine Silva Ramos ,&nbsp;Ananda Gomes de Sousa ,&nbsp;Rebeca de Siqueira Amorim ,&nbsp;Alan de Araújo Roque ,&nbsp;Israel Luís Diniz Carvalho ,&nbsp;Ana Laura Vilela de Carvalho ,&nbsp;Maiara Bernardes Marques ,&nbsp;Milena Evangelista dos Santos ,&nbsp;Moan Jéfter Fernandes Costa ,&nbsp;Luiza Rayanna Amorim de Lima ,&nbsp;Pedro Henrique Sette-de-Souza","doi":"10.1016/j.clinsp.2024.100513","DOIUrl":"10.1016/j.clinsp.2024.100513","url":null,"abstract":"<div><div>Quilombola women are primarily responsible for maintaining ethnopharmacological knowledge. Their knowledge is transmitted orally to their descendants, preserving cultural identity and ensuring the survival of Quilombola communities. Was conducting a scoping review on the use of medicinal plants by Quilombola women. Based on the PPC strategy (population, concept, and context) and the “Joanna Briggs Institute Reviewer's Manual”, this systematic scoping review sought to answer the guiding question: which medicinal plants are used for diseases that compromise women's health in Brazilian communities of African descent? The article has registered this research in the Open Science Framework available at osf.io/eb2nc, at identifier DOI <span><span>https://doi.org/10.17605/OSF.IO/5N24Y</span><svg><path></path></svg></span>. Using the search keys, 888 studies were found. After reading the titles and abstracts, 38 studies moved on to the next stage. During the reading of the texts in full, 32 were excluded for not meeting all the inclusion criteria and six studies were included in the research. In all, 44 plants, belonging to 23 families, were mentioned in the studies. The plants reported by the study have a plurality in their therapeutic use, the form of preparation, and part of the plant used. Regarding women's health, plants were recorded for sexual, menstrual, and postpartum care. The appreciation and recognition of this ancestral knowledge contribute not only to Women's health but also to strengthening the identity and rescuing the cultural roots of these communities.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100513"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The educational impact of medical students’ participation in a short-term health expedition: The Iguape one health experience","authors":"Marcelo Arruda Candido ,&nbsp;Vinicius Venturini ,&nbsp;Matheus Polly ,&nbsp;Matheus Belloni Torsani ,&nbsp;Alexandre Sizilio ,&nbsp;João Mitsuji Sakô ,&nbsp;Wen-Jan Tuan ,&nbsp;Robert Patrick Lennon ,&nbsp;Anna Sara Shafferman Levin ,&nbsp;Iolanda de Fátima Lopes Calvo Tibério","doi":"10.1016/j.clinsp.2025.100601","DOIUrl":"10.1016/j.clinsp.2025.100601","url":null,"abstract":"<div><h3>Objectives</h3><div>The primary goal of this study is to evaluate the educational effects of medical students' participation in a health expedition project. Specific objectives include assessing students' perceptions of the educational value of the program, measuring gains in clinical knowledge and skills, evaluating behavioral changes, professional orientation, and students' willingness to work within the public health system (SUS) after graduation.</div></div><div><h3>Methods</h3><div>A quantitative methodology was employed, following Kirkpatrick's four-level evaluation model. Questionnaires were administered before and after participation in the expedition to assess students' reactions, learning, and empathy. The sample comprised all 15 students who participated in the expedition, with data analysis performed using R software.</div></div><div><h3>Results</h3><div>The analysis highlighted that the expedition experience significantly impacted student's career trajectories. Approximately 80 % of participants reported that the expedition positively influenced their career decisions, and 60 % expressed interest in working within the SUS. These findings suggest that while expeditions may not yield quantifiable gains in clinical competence in the short term, they can be catalysts in forming physicians oriented towards public service and social sensitivity.</div></div><div><h3>Conclusions</h3><div>Health expeditions like the one conducted may not offer significant improvements in measurable clinical competencies in the short term but have a substantial effect on students' career decisions, aligning with public health needs and reinforcing commitment to service in vulnerable contexts. This influence suggests that practical experiences in community settings play a crucial role in shaping medical students' professional aspirations, emphasizing the importance of such programs in medical education aimed at social responsibility and health equity.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100601"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the role of MicroRNA-519d-3p in enhancing chemosensitivity of colorectal cancer cells to 5-Fluorouracil through PFKFB3 targeting","authors":"Yangyang Zhang ,&nbsp;Yiqing Zhang ,&nbsp;Yanan Xiao ,&nbsp;Shufen Xu ,&nbsp;Jie Li ,&nbsp;Juan Li ,&nbsp;Lisha Chang ,&nbsp;Jie Ding ,&nbsp;Di Wu ,&nbsp;Li Wang ,&nbsp;Guangxu Xu ,&nbsp;Keming Wang","doi":"10.1016/j.clinsp.2025.100606","DOIUrl":"10.1016/j.clinsp.2025.100606","url":null,"abstract":"<div><h3>Objective</h3><div>In the fight against Colorectal Cancer (CRC), chemotherapy resistance is a major obstacle. Therefore, it is imperative to identify effective biomarker therapeutics. Despite microRNAs (miRs) playing a crucial role in drug resistance, the mechanisms comprising miR-519d-3p's role in CRC drug resistance have not been fully understood. Therefore, the present study aimed to investigate the biological function of miR-519d-3p in the chemosensitivity of CRC cells to 5-Fluorouracil (5-FU).</div></div><div><h3>Methods</h3><div>CRC cells were treated with 5-FU and transfected. Cellular proliferation, invasion, and apoptosis were evaluated. The relationship between miR-519d-3p and 6-Phosphofructokinase-2/Frucose-2, 6-Biphosphatase-3 (PFKFB3) was analyzed, and their interaction in CRC was further investigated. In vivo tumor experiments were conducted to investigate the function of miR-519d-3p and 5-FU in CRC.</div></div><div><h3>Results</h3><div>As determined, CRC cells overexpressing miR-519d-3p were more sensitive to 5-FU in vitro, as miR-519d-3p inhibits proliferation and invasion and stimulates apoptosis. miR-519d-3p directly targeted PFKFB3. In CRC cells, PFKFB3 overexpression rescued miR-519d-3p-induced 5-FU toxicity. In vivo results showed that mice co-treated with miR-519d-3p mimics and 5-FU showed higher antitumor activity.</div></div><div><h3>Conclusion</h3><div>Overall, it may be possible to improve 5-FU chemosensitivity of CRC cells by targeting miR-519d-3p and PFKFB3.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100606"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bleeding in patients hospitalized with acute pulmonary embolism in Brazil","authors":"Leonardo Jordan Hansen Vizzotto,&nbsp;Corina dos Reis Sepeda,&nbsp;Carlos Henrique Miranda","doi":"10.1016/j.clinsp.2024.100573","DOIUrl":"10.1016/j.clinsp.2024.100573","url":null,"abstract":"<div><h3>Objective</h3><div>Acute Pulmonary Embolism (APE) is a disease with increasing incidence worldwide. Antithrombotics are the cornerstone of the treatment. Bleeding is an adverse event related to this therapy. The objective was to evaluate the prevalence of bleeding in a sample of Brazilian patients hospitalized with APE and the impact of this complication on mortality. Additionally, the performance of some bleeding predictive scores was evaluated in this sample.</div></div><div><h3>Methods</h3><div>A retrospective cohort study was carried out on patients hospitalized with APE from January 2009 through August 2017. The medical records of these patients were reviewed, and the bleeding recorded during hospital stay was classified according to the “Thrombolysis in Myocardial Infarction (TIMI) bleeding risk.” Five different predictive scores for bleeding after APE (RIETE, PE-SARD, VTE-BLEED, Kuijer, and ATRIA) were applied. Overall mortality at 30 days and one year were assessed.</div></div><div><h3>Results</h3><div>One hundred fifty-nine patients were included. The prevalence of any bleeding was 36/159 (23 %), major bleeding was 10/159 (06 %), minor bleeding was 11/159 (07 %), and bleeding requiring attention was 15/159 (10 %). Only major bleeding was associated with higher mortality at one-year follow-up with a Relative Risk (RR) of 2.00 (95 % CI 1.16–3.57; <em>p</em> = 0.044). All bleeding predictive scores evaluated showed low accuracy in identifying patients at higher risk of bleeding.</div></div><div><h3>Conclusion</h3><div>Patients hospitalized with APE in Brazil had a high prevalence of bleeding. The major bleeding increased the one-year mortality. The bleeding predictive scores assessed showed limited accuracy in identifying patients at high risk of bleeding.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100573"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142945848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of photodynamic therapy on cellular immune function in patients with cervical HPV infection","authors":"Yan Ju ,&nbsp;Qunyan Zhou","doi":"10.1016/j.clinsp.2024.100537","DOIUrl":"10.1016/j.clinsp.2024.100537","url":null,"abstract":"<div><h3>Introduction</h3><div>Cervical Human Papillomavirus (HPV) infection, a common sexually transmitted infection, can lead to cervical cancer. This study investigates the impact of Photodynamic Therapy (PDT) on cellular immune function in cervical HPV-infected patients.</div></div><div><h3>Methods</h3><div>From October 2022 to February 2023, 60 patients with cervical HPV infection were randomly assigned to a control group (conventional treatment) or a PDT group (PDT treatment), each with 30 patients. Peripheral blood samples and cervical tissue were collected before treatment, at 3 months, and at 6 months post-treatment. Flow cytometry assessed T-cell subsets (CD4⁺, CD8⁺, CD3⁺), and ELISA measured cytokine levels (TNF-α, IL-6, IL-8).</div></div><div><h3>Results</h3><div>Baseline characteristics were similar between the PDT and control groups. The PDT group showed significantly higher HPV clearance rates at 3-months (70.00 %) and 6 months (100.00 %) compared to the control group 43.33 % and 80.00 %, respectively (p &lt; 0.05). PDT significantly increased CD3⁺ and CD4⁺ cell levels at 3- and 6-months post-treatment compared to controls (p &lt; 0.05). CD8⁺ levels and the CD4⁺/CD8⁺ ratio also significantly increased in the PDT group at 6 months. Additionally, PDT significantly reduced IL-6, IL-8, and TNF-α levels at 3- and 6-months post-treatment (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>PDT enhances cellular immune function in cervical HPV-infected patients by increasing CD4⁺ and CD8⁺ T-cells and reducing pro-inflammatory cytokines. These findings highlight the potential of PDT in modulating immune responses and improving therapeutic strategies for cervical HPV infection.</div></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"80 ","pages":"Article 100537"},"PeriodicalIF":2.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}