Metabolomics in juvenile idiopathic arthritis: A distinct profile in patients under methotrexate

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Clinics Pub Date : 2025-01-01 DOI:10.1016/j.clinsp.2024.100522

Renato B. Tomioka , Gabriela R.V. Ferreira , Nadia E. Aikawa , Gustavo A.R. Maciel , José M. Soares Junior , Edmund C. Baracat , Eloisa Bonfá , Ismael Dale Cotrim Guerreiro da Silva , Clovis Almeida da Silva

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引用次数: 0

Abstract

The objective of the present study was to evaluate biochemical quantitative metabolites in peripheral blood serum samples of Juvenile Idiopathic Arthritis (JIA) patients and healthy controls. A cross-sectional study included 33 post-pubertal JIA (21 without and 12 with Methotrexate (MTX) women and 28 age-matched healthy controls. Metabolomic analyses based on targeted electrospray ionization tandem mass spectrometry were used to identify possible biochemical pathway modifications in serum from JIA patients. The mean current age (p = 0.065) was similar in JIA patients and healthy controls. Current MTX use in all subtypes of JIA patients was associated with an increase in concentrations of free carnitine [21.74 (12.7‒35.2) vs. 27.49 (14.5‒41.3) µM/L, p = 0.02], suggesting an enhanced mitochondrial metabolism and intestinal absorptive function. In contrast, a decreased mitochondrial metabolism was observed in polyarticular and systemic JIA subtypes, with a decrease of several acylcarnitines’ concentrations (p < 0.05). In conclusion, the present study identified a distinctive pattern of serum metabolic signatures in JIA patients under MTX therapy. Our findings indicate that MTX use is associated with a more efficient mitochondrial function.

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代谢组学在青少年特发性关节炎：甲氨蝶呤患者的独特概况。

本研究的目的是评估幼年特发性关节炎（JIA）患者和健康对照者外周血血清中生化定量代谢物的含量。横断面研究包括33名青春期后JIA妇女（21名未使用甲氨蝶呤，12名使用甲氨蝶呤）和28名年龄匹配的健康对照。基于靶向电喷雾电离串联质谱的代谢组学分析用于鉴定JIA患者血清中可能的生化途径改变。JIA患者和健康对照组的平均年龄（p = 0.065）相似。目前在所有亚型JIA患者中使用MTX均与游离肉碱浓度升高相关[21.74 (12.7-35.2)vs. 27.49（14.5-41.3）µM/L, p = 0.02]，提示线粒体代谢和肠道吸收功能增强。相比之下，多关节和全身JIA亚型线粒体代谢降低，几种酰基肉碱浓度降低（p < 0.05）。总之，本研究确定了甲氨蝶呤治疗下JIA患者的血清代谢特征的独特模式。我们的研究结果表明，MTX的使用与更有效的线粒体功能有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.