Clinical laboratory最新文献

{"title":"Spontaneous Retroperitoneal Hemorrhage in Anti-NXP2-Positive Dermatomyositis: a Rare but Severe Complication.","authors":"Hao Zhang, Bingbing Dai, Ningning Li","doi":"10.7754/Clin.Lab.2025.250919","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250919","url":null,"abstract":"<p><strong>Background: </strong>Spontaneous hemorrhage is a rare but serious complication of dermatomyositis (DM).</p><p><strong>Methods: </strong>We report a 61-year-old man with anti-NXP2-positive DM who developed a retroperitoneal hematoma without anticoagulation, shortly after corticosteroid initiation.</p><p><strong>Results: </strong>Laboratory investigations demonstrated rhabdomyolysis, hepatic injury and coagulopathy. The patient was managed with red blood cell transfusion, factor VIII, high-dose corticosteroids and intravenous immunoglobulin, resulting in complete recovery.</p><p><strong>Conclusions: </strong>Although hemorrhagic events in DM are most often linked to anti-MDA5 or anti-Ro52 antibodies, their association with anti-NXP2 antibodies remains uncertain. This case highlights the importance of early recognition and prompt intervention in managing spontaneous hemorrhage in DM.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Distribution of Respiratory Pathogens After the COVID-19 Pandemic.","authors":"Yeongchae Kim, Dong Ja Kim, Eon Jeong Nam, Gun Oh Chong, Incheol Seo, Nan Young Lee","doi":"10.7754/Clin.Lab.2025.250849","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250849","url":null,"abstract":"<p><strong>Background: </strong>Diverse quarantine measures against the coronavirus disease 2019 (COVID-19) pandemic have affected the distribution patterns of respiratory pathogens. This study analyzed changes in the incidence and distribution of major respiratory pathogens during the COVID-19 pandemic and after the endemic declaration. In addition, the study aimed to provide a basis for future community-centered infectious disease response strategies by identifying co-infection patterns and whether pathogens re-emerge after mitigation.</p><p><strong>Methods: </strong>In this retrospective study, the results of 105,619 respiratory pathogen tests, including FilmArray® Respiratory Panel (BioFire Diagnostics) assay (FilmArray RP), influenza tests, and COVID-19 tests, were collected at a Korean university hospital during the COVID-19 pandemic (Period I: March 2021 through April 2023) and endemic (Period II: May 2023 through February 2025). The changes in the distribution of major pathogens, age and gender characteristics, seasonality, and co-infection patterns were compared between the two periods.</p><p><strong>Results: </strong>The overall positivity rate for the FilmArray RP significantly increased from 64.4% in Period I to 71.1% in Period II (p < 0.001). In the age-specific analysis, age group 3 (4 months to 2 years) had the highest positivity rate in Period II (82.7%). Human rhinovirus/enterovirus was the most frequently detected pathogen, whereas respiratory syncytial virus cases increased in number, but decreased in proportion to all positive cases in Period II. The prevalence of adenoviruses and Mycoplasma pneumoniae has significantly increased since the pandemic. Human metapneumovirus has shifted its seasonality from autumn to summer and spring, whereas parainfluenza virus 3 has shifted from autumn to spring and summer. Among all positive cases, co-infections significantly increased to 17.6% and 22.3% during Periods I and II, respectively (p < 0.001).</p><p><strong>Conclusions: </strong>This study revealed significant changes in the incidence, seasonality, age characteristics, and co-infection patterns of respiratory pathogens after the mitigation of COVID-19. These changes result from the re-emergence of pathogens that were suppressed during the pandemic, immune vaccination, and the resumption of social activities, suggesting the need to establish an age- and pathogen-specific surveillance system for future infec-tious disease responses.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Relationship between Fibrinogen-to-Albumin Ratio and Disease Severity in Preeclamptic and Healthy Pregnant Women.","authors":"Özge Öztürk, R Taha Ağaoğlu, A Çiğdem Bayrak, Gülten Çirkin-Tekeş, Dinçer Sümer, Figen Günday, Kadriye Yakut-Yücel","doi":"10.7754/Clin.Lab.2025.250826","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250826","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to determine the predictive value of fibrinogen-to-albumin ratio (FAR) and blood urea nitrogen-to-albumin ratio (BAR) in pregnancies complicated by preeclampsia in the first trimester. Furthermore, the study evaluated their relationship with disease severity and composite perinatal adverse outcomes (CAPO).</p><p><strong>Methods: </strong>The present retrospective cross-sectional study comprised 144 pregnant women with gestational ages between 20 and 39 weeks, who were divided into three groups: mild preeclampsia (n = 48), severe preeclampsia (n = 48), and a normotensive healthy control group (n = 48). The first trimester blood samples were utilized to calculate the FAR and BAR values. Comparisons were made between the groups, and the relationship of these indices with disease severity and CAPO was evaluated.</p><p><strong>Results: </strong>FAR and BAR values were considerably elevated in both the mild and severe preeclampsia cohorts in comparison to the control group (p < 0.001). However, there were no statistically significant differences in FAR and BAR values between the mild and severe preeclampsia subgroups (p > 0.05). ROC analysis yielded moderate to high diagnostic performance for both markers in the detection of preeclampsia. Nevertheless, both FAR and BAR exhibited a lack of statistical significance in relation to the occurrence of composite perinatal adverse outcomes (CAPO), with p > 0.05.</p><p><strong>Conclusions: </strong>FAR and BAR have been identified as biomarkers that may facilitate the timely diagnosis of preeclampsia in the first trimester of pregnancy. However, since these markers do not demonstrate significant benefits in predicting the severity of the disease or adverse perinatal outcomes, further research is required in larger prospective cohorts.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Potential of microRNAs in Leukemia: a Review of Current Evidence.","authors":"Abdulmohsen A Alsanawi, Safaa M Naes, Mohammed A Alhoot, Saeed S Shaikh, Muffeed H M AlDarwish, Taha H M Aljassim, Mohammed A H Altammar, Aqeela M Alkhabbaz, Fatimah J Alzarra","doi":"10.7754/Clin.Lab.2025.250844","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250844","url":null,"abstract":"<p><strong>Background: </strong>Leukemia is a heterogeneous group of hematological malignancies with diverse clinical outcomes. microRNAs (miRNAs) have emerged as promising non-invasive biomarkers with diagnostic, prognostic, and therapeutic potential. This systematic review aims to summarize the current evidence on the role of microRNAs as di-agnostic biomarkers in different subtypes of leukemia.</p><p><strong>Methods: </strong>Following PRISMA guidelines, relevant studies were identified through a systematic search in PubMed, Scopus, and Web of Science up to August 2025. Inclusion criteria covered original research articles assessing miRNA expression in leukemia patients versus healthy controls.</p><p><strong>Results: </strong>A total of 49 studies were included. Several microRNAs (e.g., miR-155, miR-181a, miR-29b) were consistently dysregulated in acute and chronic leukemias. Patterns of expression varied by leukemia subtype and disease stage. Pooled sensitivity and specificity across studies were 0.83 and 0.92, respectively, with AUC = 0.94.</p><p><strong>Conclusions: </strong>microRNAs show high potential as diagnostic biomarkers for leukemia. However, further validation through large-scale, standardized studies is necessary before clinical application.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148789054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of MGAT3 Amplifies TGF-β Signaling to Promote Bronchiolitis Obliterans Progression.","authors":"Wei Ge, Jing Li, Yan Chen, Daxiong Zeng, Shuang Yang, Junhong Jiang","doi":"10.7754/Clin.Lab.2025.250831","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250831","url":null,"abstract":"<p><strong>Background: </strong>Bronchiolitis obliterans syndrome (BOS), a severe complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT), presents as chronic graft-versus-host disease characterized by inflammation and fibrosis of the small airway epithelium. This progressive fibrosis obstructs bronchiolar airways, leading to respiratory distress in patients. Due to the lack of effective treatments, a deeper understanding of the underlying mechanisms is crucial. This study explored the molecular mechanisms underlying the abnormal glycosylation regulation in BOS, aiming to provide new insights for early diagnosis and treatment of this disease.</p><p><strong>Methods: </strong>Bronchoalveolar lavage fluid (BALF) was collected from patients with and without BOS following allo-HSCT. N-glycans from the BALF were enriched using a solid-phase extraction method. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) was used to detect N-glycosylation characteristics in the BALF of BOS patients. This phenomenon was validated using a graft-versus-host disease (GVHD) mouse model. The impact of MGAT3 knockdown on the biological functions of airway epithelial cells (BEAS-2B) was then examined using lentivirus transfection. The molecular mechanism by which the loss of MGAT3 enhances TGF-β signaling was explored using western blotting, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence (IF).</p><p><strong>Results: </strong>Our study investigated N-glycosylation changes in BALF of BOS patients and identified a strong correlation between the loss of bisecting-GlcNAc N-glycans and BOS progression. We found a novel mechanism where MGAT3, the enzyme synthesizing bisecting-GlcNAc structures, critically regulates TGF-β signaling. MGAT3 and bisecting-GlcNAc deficiency significantly enhance TGF-β signaling by increasing TGF-β storage through upregu-lation of latent TGF-β binding protein 1 (LTBP1) and by increasing the availability of TGF-β receptors.</p><p><strong>Conclusions: </strong>Our discovery highlights the crucial role of aberrant glycosylation in BOS progression and holds significant promise for developing novel biomarkers for early diagnosis, identifying new therapeutic targets, and ultimately improving the quality of life for BOS patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Implications of Long-Term Polymerase Chain Reaction Data for Human Metapneumovirus.","authors":"Jeong Su Han, Jae-Sik Jeon, Jae Kyung Kim","doi":"10.7754/Clin.Lab.2025.250843","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250843","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to systematically examine the annual, seasonal, age-specific, and gender-specific epidemiological trends of human metapneumovirus (hMPV) infections, utilizing extensive real-world data collected over 18 years through a consistent testing methodology and analytical protocol.</p><p><strong>Methods: </strong>Real-time reverse transcription polymerase chain reaction assay results of patients admitted to Dankook University Hospital (2007 - 2024) were retrospectively analyzed. Descriptive statistics were used to assess annual, age-specific, and seasonal trends in hMPV positivity rates. We further evaluated the feasibility of using these data to establish robust epidemiological surveillance systems and standardized monitoring metrics.</p><p><strong>Results: </strong>Among 23,284 specimens, 991 (4.2%) were positive for hMPV. The highest annual positivity rates were recorded in 2007 (7.7%) and 2011 (8.1%). Children aged 2 - 11 years exhibited the highest positivity rate (6.0%), with positivity rates declining with increasing age, except for a slight increase in individuals aged ≥ 65 years. Seasonally, infections peaked in spring (11.1%), followed by summer (2.8%), winter (1.4%), and autumn (0.8%). Since the onset of the coronavirus disease 2019 (COVID-19) pandemic (2020 - 2024), the overall positivity rate dropped to 1.6% from 4.7% before the pandemic, accompanied by notable changes in seasonal patterns.</p><p><strong>Conclusions: </strong>This study revealed significant shifts in hMPV epidemiology following the COVID-19 pandemic, including reduced overall circulation, disrupted spring seasonality, and persistent impacts on high-risk pediatric populations. These findings provide evidence to optimize diagnostic testing for hMPV while providing actionable evidence to adjust optimal diagnostic testing windows, enhance infection prevention strategies, and implement focused monitoring of high-risk populations. Specifically, hMPV testing should be prioritized for pediatric patients during the typical peak months (March - May) and expanded to include atypical winter peaks (December - February) in the post-pandemic era. For older adults (≥ 65 years), targeted testing during winter months may enhance diagnostic yield and support timely clinical management.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perception of Medical Laboratory Professionals on the Role of Artificial Intelligence in Advancing Hematology Diagnostics.","authors":"Talal Qadah","doi":"10.7754/Clin.Lab.2025.251103","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.251103","url":null,"abstract":"<p><strong>Background: </strong>Artificial Intelligence (AI) has grown quickly in healthcare and has had a big effect on medical laboratory diagnostics, especially when it comes to diagnosing blood disorders. To figure out the pros and cons of using AI in hematology diagnostics, it is important to understand the perspective of medical laboratory workers instead of specialists toward AI use in hematology laboratory diagnostics. The goal of this study was to investigate what medical lab workers now know about AI and how they think it affects the accuracy of diagnostic hematology and patient outcomes.</p><p><strong>Methods: </strong>Methods involved 113 participants, mostly laboratory technologists who filled out a standardized questionnaire as part of a quantitative exploratory research design. The data collection phase lasted 6 months, during which time-informed consent was sought and reminders were provided to boost response rates. Statistical tests were used including t-tests and regression analysis to determine the links between demographic factors and workers opinions on AI in hematological diagnoses.</p><p><strong>Results: </strong>The primary findings of this study dwell within the role of job skill and gender influence on \"attitude\", specifically, the study found that most of the participants believe that their professional attitude to adoption of AI in diagnoses could make their job more accurate and faster. However, there were several concerns about the quality of data, how easy it will be to understand the model, and the moral implications. There were positive relationships between knowledge, attitudes, and practices linked to AI. The secondary outcomes showed that most of the participants were young, with 45.1% being between the ages of 30 and 39. In addition, 65.5% of them had bachelor's degree, which suggests that they were comfortable with technology. Some of the participants had less than ten years of work experience, while others had more than ten years. Statistical tests demonstrated that demographic characteristics are quite important in how medical laboratory workers think about AI.</p><p><strong>Conclusions: </strong>Medical laboratory workers believe that the use of AI in hematology diagnostics has its benefits, but they need more training and assistance to deal with their fears and create a space where human expertise and AI technologies can work together. By taking these views into account, healthcare organizations may better educate their staff for the changing role of AI in diagnostics, which will lead to improved patient outcomes and satisfaction.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological Effects of the Co-Inheritance of Glucose-6-Phosphate Dehydrogenase Deficiency and Sickle Cell Disease.","authors":"Michael H Mezgebe, Nazeer Alli, Aamina Moosa, Susan Louw","doi":"10.7754/Clin.Lab.2025.250838","DOIUrl":"https://doi.org/10.7754/Clin.Lab.2025.250838","url":null,"abstract":"<p><strong>Background: </strong>Glucose-6-phosphate dehydrogenase deficiency (G6PDD) and sickle cell disease (SCD) both result in hemolysis associated with anemia. Studies on the hematological effects of SCD and G6PDD co-inheritance are limited, with conflicting results. The objective of the study was to compare the hematological parameters and biochemical markers of hemolysis viz., lactate dehydrogenase (LDH) and unconjugated bilirubin (UBR), in patients with SCD to patients with SCD/G6PDD co-inheritance.</p><p><strong>Methods: </strong>A total of 214 patients with SCD including both homozygous (HbSS) and heterozygous (HbAS) variants, with or without concomitant G6PDD were identified over a 14-year period at Charlotte Maxeke Johannesburg Academic Hospital (CMJAH) in South Africa. SCD was diagnosed with high-performance liquid chromatography (HPLC), while G6PD status was determined with G6PD spot testing. The full blood count (FBC) parameters, along with the UBR and LDH levels, were recorded.</p><p><strong>Results: </strong>The median ages at diagnosis were 4.5 and 20 years for HBSS (with and without G6PDD) and HBAS (with and without G6PDD), respectively. The FBC parameters viz., red blood cell count (RCC), hematocrit (Hct), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC) and mean cell volume (MCV), as well as UBR levels, showed no significant differences between patients with HbSS alone and those with HbSS/ G6PDD. However, patients with HbAS/G6PDD had significantly higher mean UBR levels (p < 0.05) in comparison to those with HbAS alone, although the FBC parameters showed no statistically significant differences between these groups.</p><p><strong>Conclusions: </strong>Co-inheritance of G6PDD with HbAS does not result in anemia, but elevated biochemical markers of hemolysis suggest ongoing hemolysis. This raises concern about potential complications associated with chronic hemolysis, beyond anemia.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Case of a Newborn with Blocked RhD Antigen Caused by Low-Titer Antibody.","authors":"Ling Li, Se Zhen, Danzeng Bianjue, Xueni Chen","doi":"10.7754/Clin.Lab.2025.250850","DOIUrl":"10.7754/Clin.Lab.2025.250850","url":null,"abstract":"<p><strong>Background: </strong>Hemolytic disease of the fetus and newborn (HDFN) is a condition characterized by severe hemolysis and anemia in fetuses or neonates. To our knowledge, this is the first reported case of a newborn with blocked RhD antigen caused by low-titer antibody.</p><p><strong>Methods: </strong>Peripheral blood samples were collected from the newborn upon admission. ABO and RhD blood typing was performed using a microcolumn gel agglutination test. Direct antiglobulin test (DAT) was performed using polyspecific anti-human globulin. Indirect antiglobulin test (IAT) was used to screen for irregular antibodies in the serum. Thermal elution was carried out by incubating the newborn's red blood cells at 56℃ for 10 minutes, and the resulting eluate was tested for non-ABO antibodies and their titers by IAT.</p><p><strong>Results: </strong>At birth, the infant was typed as O Rh-positive. However, pre-transfusion retesting indicated O Rh-negative status. The DAT was negative, whereas the IAT was positive (2+). Subsequent ABO-compatible elution testing also yielded a positive result (1+). The antibody titer was measured at 1:16.</p><p><strong>Conclusions: </strong>This is the first reported case of blocked RhD antigen caused by low-titer antibody. This case high-lights the critical importance of comprehensive serological investigation in affected neonates to guide appropriate clinical management and transfusion strategy.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics and Diagnosis of Myelodysplastic Syndrome Accompanied by Eosinophilia and Basophilia.","authors":"Jianjun Gao, Junjuan Qi, Ruimin Li, Xiaofang Zhang","doi":"10.7754/Clin.Lab.2025.250928","DOIUrl":"10.7754/Clin.Lab.2025.250928","url":null,"abstract":"<p><strong>Background: </strong>The aim was to explore the clinical characteristics, diagnostic methods and prognosis of Myelodysplastic Syndromes (MDS) accompanied by eosinophilia and basophilia.</p><p><strong>Methods: </strong>The clinical data of a patient with MDS accompanied by eosinophilia and basophilia were retrospectively analyzed, including the patient's clinical manifestations, blood routine, bone marrow image, flow cytometry, and molecular biological examination results, and discussed in combination with relevant literature.</p><p><strong>Results: </strong>The patient is a middle-aged female who was admitted to the hospital due to fatigue and dizziness for more than two months. The blood routine test indicated pancytopenia, an increased percentage of eosinophils, and an increased percentage of basophils. The bone marrow image showed active proliferation of nucleated cells, obvious pathological hematopoiesis can be seen, and the proportion of eosinophils and basophils increased. Flow cytometry results showed that the proportion of myeloid primitive naive cells increased, the expression of CD34 was enhanced, the expression of CD117 in some cells was weakened, the phenotype was abnormal. The proportion of granulocytes decreased, the proportion of eosinophils and basophils increased, and the expression of CD36 in some erythroid cells was weakened. Molecular biology examination revealed mutations in the IDH1 gene, SRSF2 gene, SETBP1 gene, ETV6 gene, ASXL1 gene and DNMT3A gene. Based on the patient's clinical manifestations and various examination results, the diagnosis was myelodysplastic syndrome with increased eosinophils and basophils. After the corresponding treatment was given, the patient's symptoms were relieved and the blood routine indicators improved compared with before.</p><p><strong>Conclusions: </strong>MDS accompanied by eosinophilia and basophilia is a rare type of leukemia. Clinical manifestations are not specific. Diagnosis requires a combination of blood routine, bone marrow image, flow cytometry, and molecular biological examination. This type of patient has a relatively poor prognosis, especially those with gene mutations. Early and clear diagnosis and the selection of appropriate treatment plans based on the stratification of patients' risk levels, are of great significance for improving the prognosis of patients.</p>","PeriodicalId":10384,"journal":{"name":"Clinical laboratory","volume":"72 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}