Clinical science and molecular medicine最新文献_第8页

The nature of inactive renin in human plasma and amniotic fluid. 人血浆和羊水中失活肾素的性质。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550041

A A Shulkes, R R Gibson, S L Skinner

{"title":"The nature of inactive renin in human plasma and amniotic fluid.","authors":"A A Shulkes, R R Gibson, S L Skinner","doi":"10.1042/cs0550041","DOIUrl":"https://doi.org/10.1042/cs0550041","url":null,"abstract":"1. The properties of inactive and active renin in human plasma and amniotic fluid were studied chromatographically. Activation was achieved at pH 3.3 with and without added pepsin. 2. Acid activation of renin was time- and temperature-dependent but was inhibited by dilution of the sample. The dilution effect was corrected by adding pepsin. Such characteristics indicate that activation at low pH is catalysed by intrinsic enzymes. 3. Separation and/or dilution of the activating enzyme during ion-exchange chromatography concealed the eluted position of inactive renin and reduced the amount recovered. Only after full activation of the eluted renin was achieved with added pepsin was a distinct peak of inactive renin exposed. 4. At pH 7.5 inactive renin carried a lower negative charge than the active enzyme. This charge difference was lost after activation. 5. No molecular-weight differences between active, inactive renin or the International Renin Standard were detected by gel filtration. No renin of larger molecular weight was present. 6. These findings will be helpful in purification studies of human inactive renin.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"41-50"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11250186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Renin release from renin granules in the dog. 狗肾素颗粒中肾素的释放。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550011

S Funakawa, T Higashio, K Yamamoto

引用次数: 10

Effects of concentration-polarization on the filtration of proteins through filters constructed from isolated renal basement membrane. 浓度极化对蛋白质通过离体肾基底膜滤器过滤的影响。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550113

T G Cotter, G B Robinson

{"title":"Effects of concentration-polarization on the filtration of proteins through filters constructed from isolated renal basement membrane.","authors":"T G Cotter, G B Robinson","doi":"10.1042/cs0550113","DOIUrl":"https://doi.org/10.1042/cs0550113","url":null,"abstract":"1. Filters comprising multiple layers of rabbit renal tubular basement membrane were constructed with conventional pressure filtration chambers. The effects of concentration-polarization on the behaviour of these filters was assessed by studying the filtration of proteins and of serum under turbulent (stirred) and unstirred conditions. 2. With stirring bovine serum albumin was effectively rejected by the filter barriers (sigma = 0.95) but rejection was diminished (sigma = 0.18) without stirring. The hydraulic permeability of the filters also fell without stirring. 3. In the presence of horse immunoglobulin G, a wholly rejected protein, the rejection of cytochrome c was increased and hydraulic flux was reduced. 4. Filtration studies of serum showed that serum protein was effectively rejected with stirring (sigma greater than 0.999) but rejection diminished when stirring ceased (sigma = 0.98). Albumin was the only protein detected in the filtrate with stirring but alpha- and beta- globulins appeared when stirring ceased. 5. These results show that concentration-polarization markedly affects the behaviour of these basement membrane filters in vitro, since without stirring a polarization layer of rejected protein is formed, which reduces hydraulic permeability and results in increased protein permeation through the filter.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"113-9"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11869330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Definition of a conjugation of dysfunction in Gilbert's syndrome: studies of the handling of bilirubin loads and of the pattern of bilirubin conjugates secreted in bile. 吉尔伯特综合征功能障碍偶联的定义:胆红素负荷处理和胆红素偶联物在胆汁中分泌模式的研究。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550063

C A Goresky, E R Gordon, E A Shaffer, P Paré, D Carassavas, A Aronoff

{"title":"Definition of a conjugation of dysfunction in Gilbert's syndrome: studies of the handling of bilirubin loads and of the pattern of bilirubin conjugates secreted in bile.","authors":"C A Goresky, E R Gordon, E A Shaffer, P Paré, D Carassavas, A Aronoff","doi":"10.1042/cs0550063","DOIUrl":"https://doi.org/10.1042/cs0550063","url":null,"abstract":"1. Intravenous doses of bilirubin (3.4 mumol/kg) were given to normal subjects and patients with Gilbert's syndrome. Both groups displayed an identical initial disappearance of a substantial proportion of the bilirubin but, late in time, the Gilbert's patients exhibited reduced clearance with a sustained elevation of the plasma bilirubin and no reflux into the plasma space of conjugated bilirubin. Increasing the dose in normal subjects (by factors of 3 and 6) failed to reproduce the response found in the Gilbert's patients. 2. In the the bile-containing duodenal aspirates of Gilbert's patients the average proportion of bilirubin found as bilirubin diglucuronide was 68% (normal 88%) and of bilirubin monoglucuronide, 23% (normal 7%). Both differences were significant at the P less than 0.001 level. In the Gilbert's patients restriction of caloric intake to 1569 kJ/day for 2 days characteristically raised the serum bilirbuin with no modification of the biliary pigment pattern; phenobarbital (180 mg/day for 2 weeks) decreased the plasma bilirubin to the normal range with, concomitantly, a reversion of the biliary pigment pattern towards normal. 3. We conclude that there is no hepatic uptake defect in Gilbert's syndrome but that there is decreased activity in the conjugation process underlying the addition of the second glucuronic acid moiety to bilirubin, to form bilirubin diglucuronide.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11870018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Impaired skeletal muscle vasomotor response to infused noradrenaline in baboons with obstructive jaundice. 阻塞性黄疸狒狒输注去甲肾上腺素后骨骼肌血管运动反应受损。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550109

L Bomzon, P B Wilton, T A McCalden

引用次数: 17

Thyroid-stimulating hormone: neuroregulation and clinical applications. Part 1. 促甲状腺激素:神经调节及临床应用。第1部分。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550001

M F Scanlon, B Rees Smith, R Hall

引用次数: 4

Blood alcohol and impairment of judgment. 血液酒精和判断障碍

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550057

J E Pohl

引用次数: 6

Changes in the collagen of synovial membrane in rheumatoid arthritis and effect of D-penicillamine. 类风湿关节炎滑膜胶原蛋白的变化及d -青霉胺的作用。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550031

C R Lovell, A C Nicholls, M I Jayson, A J Bailey

引用次数: 25

Revised standards for normal resting dead-space volume and venous admixture in men and women. 男性和女性正常静息死区容积和静脉混合物修订标准。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550125

E A Harris, E R Seelye, R M Whitlock

引用次数: 20

Catabolic rate of alpha1-antitrypsin of of Pi types S, and MMalton and of asialylated M-protein in man. α - 1-抗胰蛋白酶在人体内的分解代谢率。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550103

J O Jeppsson, C B Laurell, B Nosslin, D W Cox

{"title":"Catabolic rate of alpha1-antitrypsin of of Pi types S, and MMalton and of asialylated M-protein in man.","authors":"J O Jeppsson, C B Laurell, B Nosslin, D W Cox","doi":"10.1042/cs0550103","DOIUrl":"https://doi.org/10.1042/cs0550103","url":null,"abstract":"1. Human alpha1-antitrypsin was isolated with preserved microheterogeneity from subjects of Pi types M, S and MMalton. The M-protein was partially (20%) and completely desialylated. The proteins were labelled with either 125I or 131I. 2. The disappearance rate of these alpha1-antitrypsins was studied after simultaneous injection of the two types of labelled protein into Pi M subjects. The fractional catabolic rates of S- and MMalton-protein were 0.36 and 0.34 day(-1) respectively compared with 0.28 day(-1) for M-protein. The ratio of extravascular to plasma pools was 1.4 for S- protein and 1.6 for MMalton-protein. The 20% desialylated M-protein showed an increase of about 100% in its fractional catabolic rate. The disappearance rate of completely desialylated alpha1-antitrypsin was extremely rapid. 3. The slightly higher fractional catabolic rate of S- than of M-protein can only partly explain the 40% lower plasma concentration in subjects of Pi type S. Similarly the slight increase in catabolic rate of Pi MMalton- protein is too small to explain why the alpha1-antitrypsin content of the blood in Pi MMalton subjects is only 15% of that normally found. A low hepatic secretion seems to be the major cause of the low alpha1-antitrypsin concentration found in subjects of types Pi S and MMalton, as in Pi type Z.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"103-7"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11521182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26