Clinical science and molecular medicine最新文献_第7页

Analytical subcellular fractionation studies on enterocytes from the jejunum and ileum of the rat and some properties of brush-border alkaline phosphatase. 大鼠空肠和回肠肠细胞亚细胞分离分析及刷缘碱性磷酸酶的一些特性研究。

Clinical science and molecular medicine Pub Date : 1978-08-01 DOI: 10.1042/cs0550157

R M Batt, T J Peters

{"title":"Analytical subcellular fractionation studies on enterocytes from the jejunum and ileum of the rat and some properties of brush-border alkaline phosphatase.","authors":"R M Batt, T J Peters","doi":"10.1042/cs0550157","DOIUrl":"https://doi.org/10.1042/cs0550157","url":null,"abstract":"1. Enterocytes, isolated from the proximal jejinum and distal ileum of the rat, were homogenized and their organelles separated by isopycnic centrifugation on continuous sucrose density gradients. The distributions of marker enzymes for the principal organelles, RNA and protein were determined in the sucrose gradients and related to the activities per entercocyte. 2. In the jejunum the modal equilibrium densities of the various organelles were: brush borders (1.20), lysosomes (1.20), peroxisomes (1.19), mitochondria (1.17) and basal-lateral membranes (1.13). The values were not significantly different in the ileum. The activities of brush-border enzymes, soluble and mitochondrial malate dehydrogenase, soluble and membrane-associated lactate dehydrogenase and particulate protein content, however, were greater in the jejunal than the ileal enterocytes. 3. Detergent exposed latent alkaline phosphatase activity in jejunal enterocytes and indicated that this enzyme is present not only in the brush border but also in the basal-lateral membrane and soluble fractions of the cell. 4. Isolated jejunal brush-border preprations showed latent activities of both alkaline phosphatase and gamma-glutamyltransferase whereas the activities of alpha-glucosidase and leucyl-beta-naphylamidase were not affected by detergent. Mechanical disruption of these preparations suggested the presence of two forms of alkaline phosphatase in the brush border and provides a technique to assess membrane fragility.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 2","pages":"157-65"},"PeriodicalIF":0.0,"publicationDate":"1978-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11251395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effect of varying PCO2 on intracellular pH and lactate consumption in the isolated perfused rat liver. 不同二氧化碳分压对离体灌注大鼠肝脏细胞内pH和乳酸消耗的影响。

Clinical science and molecular medicine Pub Date : 1978-08-01 DOI: 10.1042/cs0550175

P G Baron, R A Iles, R D Cohen

引用次数: 22

Intestinal cholecalciferol absorption in the elderly and in younger adults. 老年人和年轻人肠道胆骨化醇的吸收。

Clinical science and molecular medicine Pub Date : 1978-08-01 DOI: 10.1042/cs0550213

J M Barragry, M W France, D Corless, S P Gupta, S Switala, B J Boucher, R D Cohen

引用次数: 131

Intestinal absorption and tissue retention of cadmium and calcium in normal adult rats and rats given an active metabolite of vitamin D (1,25-dihydroxycholecalciferol). 正常成年大鼠和给予活性维生素D代谢物(1,25-二羟基胆钙化醇)的大鼠的镉和钙的肠道吸收和组织保留。

Clinical science and molecular medicine Pub Date : 1978-08-01 DOI: 10.1042/cs0550195

R Lorentzon, S E Larsson

引用次数: 5

Peptide excretion in experimental Fanconi syndrome in the rat. 实验性范可尼综合征大鼠的肽排泄。

Clinical science and molecular medicine Pub Date : 1978-08-01 DOI: 10.1042/cs0550205

A M Asatoor, M R Bending, M D Milne

引用次数: 4

Lack of effect of blood-volume expansion on short-circuit current across an isolated toad skin incorporated in the circulation of a rat. 血容量扩张对大鼠循环中孤立蟾蜍皮肤的短路电流缺乏影响。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550015

D Querido, L C Isaacson

引用次数: 1

Characterization of beta-adrenergic receptor linked to adenylate cyclase in a human cancer cell line (COLO 16). 人类癌细胞系中腺苷酸环化酶相关β -肾上腺素能受体的表征(COLO 16)。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550023

T J Martin, S R Nahorski, N H Hunt, J K Dawborn, R S Loomes, C E Underwood

{"title":"Characterization of beta-adrenergic receptor linked to adenylate cyclase in a human cancer cell line (COLO 16).","authors":"T J Martin, S R Nahorski, N H Hunt, J K Dawborn, R S Loomes, C E Underwood","doi":"10.1042/cs0550023","DOIUrl":"https://doi.org/10.1042/cs0550023","url":null,"abstract":"1. A human cancer cell line (COLO 16) derived originally from an epidermal squamous cell carcinoma was found to possess adenylate cyclase responsiveness to beta-adrenergic agonists. 2. The adenylate cyclase response was characterized with respect to activation constants (KA) for various beta-adrenergic agonists and inhibition constants (Ki) for antagonists. 3. Intact cells responded with dose-dependent increases in production of cyclic adenosine 3':5'-monophosphate. 4. Properties of the beta-adrenergic receptor were evaluated by using the specific binding of [3H]propranolol to cell membranes. Specific binding was saturable, with KD 5.79 nmol/l and binding sites 0.68 pmol/mg of protein. 5. Competition for binding to cell membranes was shown by beta-adrenergic agonists and antagonists and was stereospecific. There was close agreement between the affinity of these various agents on adenylate cyclase and receptor binding. 6. It is likely that the beta-adrenergic receptor-linked adenylate cyclase in COLO 16 cells represents persistence in a cancer cell line of a receptor present normally in epidermal cells.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"23-9"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11250185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Exchange of metabolites in the leg of exercising juvenile diabetic subjects. 青少年糖尿病患者运动时腿部代谢物的交换。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550073

J Lyngsøe, J P Clausen, J Trap-Jensen, L Sestoft, O Schaffalitzky de Muckadell, J J Holst, S L Nielsen, J F Rehfeld

引用次数: 17

Gastric ulcers with and without associated duodenal ulcer have different pathophysiology. 胃溃疡合并和不合并十二指肠溃疡具有不同的病理生理。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550097

S K Lam, C L Lai

{"title":"Gastric ulcers with and without associated duodenal ulcer have different pathophysiology.","authors":"S K Lam, C L Lai","doi":"10.1042/cs0550097","DOIUrl":"https://doi.org/10.1042/cs0550097","url":null,"abstract":"1. Maximal acid output after pentagastrin stimulation, and fasting and postprandial serum gastrin concentrations were determined in 25 normal subjects, 30 patients with corpus gastric ulcers, 10 patients with prepyloric ulcers and 30 patients with both duodenal and gastric ulcers. 2. Corpus ulcers and prepyloric ulcers formed one distinct group. Maximal acid output was abnormally low in the corpus ulcer patients and no different from normal in prepyloric ulcer patients, whereas fasting serum gastrin and postprandial integrated gastrin response was abnormally high in the former and no different from the normal in the latter. Furthermore, as in the normal subjects, a significant negative correlation between maximal acid output expressed in mmol h(-1) kg(-1) body weight and postprandial integrated gastrin response was observed in the corpus and prepyloric ulcer patients taken as a group. 3. In complete contrast patients with both duodenal and gastric ulcers, in whom postprandial integrated gastrin response was statistically highest amongst the three types of gastric ulcers, had a significantly positive correlation between maximal acid output and the integrated gastrin response. 4. These findings suggest the operation of different pathophysiological mechanisms in gastric ulcers with and without associated duodenal ulcers.","PeriodicalId":10356,"journal":{"name":"Clinical science and molecular medicine","volume":"55 1","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1042/cs0550097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11870021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Catecholamines in peripheral venous plasma in patients on chronic haemodialysis. 慢性血液透析患者外周静脉血浆儿茶酚胺含量。

Clinical science and molecular medicine Pub Date : 1978-07-01 DOI: 10.1042/cs0550089

B P McGrath, J G Ledingham, C R Benedict

引用次数: 99