Chronobiology International最新文献

{"title":"Ang II-induced oscillation of clock genes can attenuate phenotypic transformation in vascular smooth muscle cells by activating the AT1R/PLC/Ca²⁺/PKC/p-CREB pathway.","authors":"Conglan Ji, Tao Ge, Nan Wang, Kai Guo, Jun Liu, Kui Yang","doi":"10.1080/07420528.2025.2523515","DOIUrl":"10.1080/07420528.2025.2523515","url":null,"abstract":"<p><p>This study aimed to investigate the role of clock genes Per1/Per2 in angiotensin II (Ang II)-induced vascular smooth muscle cells (VSMCs) phenotypic transformation and the underlying mechanisms. Primary rat VSMCs were treated with Ang, valsartan or other inhibitors. Assays included PCR, Western blot (Per1, Per2, p-MLC₂₀, p-CREB, AT1R), cell viability (MTT, Ki67, binuclear count), cell cycle, calcium and IP3. 50% fetal bovine serum shock significantly reduced the proliferation-promoting effect of Ang. Ang significantly increased the expression of Per1/Per2 mRNA at ZT3 but decreased it at ZT19 and ZT23 correlating with p-MLC₂₀ changes. Valsartan (AT1R inhibitor), Calphostin C (PKC inhibitor), U73122 (PLC inhibitor), 2-APB (IP3R blocker) and dantrolene sodium salt (Calcium channel protein inhibitor) significantly blocked the effects of Ang on Per1/Per2 genes. Ang significantly increased the p-CREB expression, IP3 and [Ca²⁺]i concentration transiently but decreased it in the long term. However, Ang significantly decrease Per1, Per2, and AT1R proteins expression transiently but increased it in the long term. Finally, silencing Per1 and Per2 enhances Ang-induced proliferation of VSMCs. Ang II triggers Per1/Per2 oscillation via the AT1R/PLC/Ca²⁺/PKC/p-CREB axis. Remarkably, the resultant AT1R-Per1/Per2 feedback loop counteracts Ang II-driven VSMC phenotypic transformation.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1025-1038"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circadian gene polymorphisms and cancer: Insights into development and risk.","authors":"Danixa Llanos, Kevins Jara-Medina, Luis Lillo, Gerardo Cabello-Guzman, Constanza Lagunas, Francisco J Valenzuela-Melgarejo","doi":"10.1080/07420528.2025.2522816","DOIUrl":"10.1080/07420528.2025.2522816","url":null,"abstract":"<p><p>The modern lifestyle imposes new stressors on our physiology, disrupting the natural adaptability and timekeeping abilities regulated by light exposure during dark hours. These chronodisruptors affect the timing of metabolic and hormonal processes, potentially interfering with homeostasis and cellular functions such as the cell cycle. Increasing evidence links such circadian disruption to an increased risk of diseases, notably cancer. Genetic variations can also influence this disturbance in circadian clock genes, which form the core components of the circadian rhythm regulatory system, including BMAL-1, CLOCK, PER 1-3, CRY 1-2, RORA, NPAS2, and TIMELESS. This review explores the potential of these polymorphisms in contributing to the timing disruptions in circadian rhythms, impacting cell cycle checkpoints like WEE1, Cyclins, and C-MYC. Further investigations into the genetic components and their relationship with cancer risk are critical for understanding their intricate connections with the circadian system and identifying novel molecular pathways that could be utilized for cancer diagnosis and prevention.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"977-993"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of social jet lag in obstructive sleep apnea syndrome (OSAS).","authors":"Mehmet Hakan Bilgin, Pınar Guzel Ozdemir","doi":"10.1080/07420528.2025.2521349","DOIUrl":"10.1080/07420528.2025.2521349","url":null,"abstract":"","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"975-976"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of natural orexin 2 receptor antagonists from Valeriana species: A potential approach for insomnia treatment.","authors":"Aparna G Shenoy, Vishal Ravi, Suhail Subair, Durairaj Ragu Varman, Rajesh Raju, Niyas Rehman","doi":"10.1080/07420528.2025.2529398","DOIUrl":"10.1080/07420528.2025.2529398","url":null,"abstract":"<p><p>The orexinergic system, comprising orexin-A and orexin-B neuropeptides that bind to OX1R and OX2R receptors, plays a critical role in regulating sleep-wake cycles, appetite, and alertness. OX2R is particularly important for promoting arousal and non-rapid eye movement (NREM) sleep and has been linked to sleep disorders such as insomnia and narcolepsy. Although OX2R antagonists like suvorexant have shown therapeutic promise, they are often associated with side effects including cognitive impairment and dependence, highlighting the need for safer alternatives. This study employed an <i>in-silico</i> approach to identify natural OX2R antagonists from Valeriana species. Phytochemicals were screened based on molecular docking and favourable ADME/T profiles. Molecular dynamics (MD) simulations and post-MD analysis confirmed stable binding of hesperidine and valerosidate to OX2R. Principal component analysis (PCA) revealed minimal conformational variability while gibbs free energy landscape (FEL) analysis and MM-PBSA binding free energy calculations further supported the strong binding of hesperidine and valerosidate to OX2R, comparable to suvorexant. These findings support hesperidine and valerosidate as promising, naturally derived OX2R antagonists, and warrant further invitro and invivo investigations for potential therapeutic application in insomnia treatment.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1107-1120"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mental health and well-being in Chinese nurses: Relationship with psychological flexibility and night shifts.","authors":"Ying Wu, Lijuan Dong, Xiaofang Jiang, Xinhong Li, Juan Han, Hongmei Lin","doi":"10.1080/07420528.2025.2507226","DOIUrl":"10.1080/07420528.2025.2507226","url":null,"abstract":"<p><p>The purpose of this study was to investigate whether the relationship between mental health and well-being among Chinese nurses could be mediated by factors such as night shift frequency and psychological flexibility. A total of 421 nurses were selected from 10 Grade secondary public hospitals in Shanghai, China. The 12-item General Health Questionnaire (GHQ-12) was used to assess mental health status. Additionally, psychological flexibility and well-being were assessed using the Chinese version of Personalized Psychological Flexibility Index and Chinese version of the 5-item WHO Well-Being Index, respectively. Correlation analysis showed that psychological flexibility was positively associated with well-being (<i>r</i> = 0.220, <i>p</i> < 0.05) and negatively correlated with mental health issues (<i>r</i> = ‒0.225, <i>p</i> < 0.05). Night shift frequency was linked to mental health issues (<i>r</i> = 0.276, <i>p</i> < 0.05) and lower psychological flexibility (<i>r</i> = ‒0.165, <i>p</i> < 0.05). Structural equation modeling demonstrated that age had direct positive effects on psychological flexibility (β = 0.130) and mental health (β = 0.541), while night shift frequency directly worsened mental health (β = 0.618) and reduced psychological flexibility (β = ‒0.198). Psychological flexibility significantly influenced both mental health (β = ‒0.674) and well-being (β = 0.418), with indirect effects on well-being (β = 0.268). Mental health was the strongest predictor of well-being (β = 0.718). These findings highlight the importance of psychological flexibility, night shifts and age in nurses' mental health well-being, though the study's cross-sectional design limits causal inferences.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"994-1001"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of prolonged exposure to different lighting conditions on MPTP-induced motor dysfunction and cognitive performance in male rats.","authors":"Hamideh Rahmati, Seyedeh Masoumeh Seyedhosseini Tamijani, Hamed Ghazvini, Behzad Garmabi, Ali Siahposht-Khachaki, Saba Niknamfar, Raheleh Rafaiee","doi":"10.1080/07420528.2025.2529343","DOIUrl":"10.1080/07420528.2025.2529343","url":null,"abstract":"<p><p>Circadian rhythm disruption has been increasingly implicated in neurodegenerative diseases, including Parkinson's disease (PD). While motor impairments in PD are well-established, the impact of chronic circadian misalignment on both motor and cognitive dysfunctions remains underexplored. This study investigates the effect of prolonged exposure to different lighting conditions on MPTP-induced motor and cognitive deficits in a rat model. A total of 100 adult male Wistar rats were divided into five groups with different light-dark cycle conditions: standard 12-h light/12-h dark (12 L:12D), constant light (24 L), constant darkness (24D), 20-h light/4-h dark (20 L:4D), and 4-h light/20-h dark (20D:4 L). After 60 d of exposure to these conditions, each group was further divided into Parkinsonian (MPTP-treated) and non-Parkinsonian (saline-treated) subgroups. MPTP hydrochloride (25 µg/kg) was administered intranasally to induce Parkinsonism. Behavioral assessments, including the beam balance test, open field test, Y-maze test, and shuttle box test, were conducted to evaluate motor coordination and cognitive function. Immunohistochemical analyses of α-synuclein (α-syn) accumulation in the substantia nigra (SNc) and tyrosine hydroxylase (TH) expression in the suprachiasmatic nucleus (SCN) were performed. Data were analyzed using t test, one-way ANOVA and Tukey's post hoc test. MPTP administration significantly impaired motor function, as evidenced by increased crossing time in the beam balance test (<i>p</i> < 0.001) and reduced locomotor activity in the open field test (<i>p</i> < 0.01). Prolonged exposure to different lighting conditions further exacerbated these deficits, particularly in the 24 L and 20 L:4D groups (<i>p</i> < 0.0001). Cognitive assessments revealed that MPTP-treated rats exhibited marked deficits in working memory (Y-maze, <i>p</i> < 0.05) and associative learning (shuttle box, <i>p</i> < 0.01), with the most severe impairments observed in groups subjected to extreme light-dark alterations. Immunohistochemical analyses demonstrated significantly increased α-syn accumulation in the SNc (<i>p</i> < 0.0001) and decreased TH expression in the SCN (<i>p</i> < 0.001), particularly in MPTP-treated rats under photoperiod disruption. Chronic photoperiod disruption exacerbates MPTP-induced motor dysfunction, cognitive impairment, and neurodegeneration. Targeting circadian regulation may offer a potential therapeutic approach for mitigating neurodegenerative progression in PD.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1080-1095"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of glucose supplementation on physiology and metabolism of a nocturnal migrant (<i>Emberiza bruniceps</i>) under laboratory conditions.","authors":"Anupama Yadav, Ranju Chetri, Anand S Dixit, Sangeeta Rani","doi":"10.1080/07420528.2025.2529348","DOIUrl":"10.1080/07420528.2025.2529348","url":null,"abstract":"<p><p>Glucose is the simplest sugar and the ultimate source of energy for the cells. In the present study, we have tried to investigate the plausible role of glucose on seasonal physiology of redheaded bunting such as: hyperphagia, initiation and persistence of migratory restlessness, life history state-dependent plasma metabolite profile and fuel preference during migratory phenotype. Buntings are photosensitive species and exposure to short (8 L:16D) and long (13 L:11D) photoperiod leads to simulation of non-migratory and migratory life history states, respectively, in these birds. For experiment, birds were divided into three groups (<i>N</i> = 5 each), where, group I served as control, group II and III received 5% and 15% glucose (w/v), respectively, in drinking water. Our results clearly demonstrate the role of glucose in early initiation of <i>zugunruhe</i> (migratory restlessness) followed by its sustenance for longer duration of time. Food intake was inversely proportional to glucose concentration; however, water intake was substantially high for birds of 5% glucose group. Besides these, the circulating plasma concentration of glucose, triglycerides and cholesterol were significantly high because of external glucose supplementation. The study also provides the evidence of non-dependence of fat alone in fueling the migratory journey. Thus, the current study clearly illustrates the role of glucose in different life history states of migratory redheaded bunting.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1096-1106"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the Arabic version of the reduced Horne-Östberg morningness-eveningness questionnaire (rMEQ).","authors":"Roni Chaaya, Rebecca Cattan, Christele Lahoud, Debora Tornquist, Felipe Barreto Schuch, Georges Merhi, Georges-Junior Kahwaji, Rachele Lahoud, Hanna Mattar, Sahar Obeid, Souheil Hallit, Feten Fekih-Romdhane","doi":"10.1080/07420528.2025.2525465","DOIUrl":"10.1080/07420528.2025.2525465","url":null,"abstract":"<p><p>Individual differences in sleep-wake cycles give rise to \"morningness-eveningness.\" The assessment of morningness-eveningness is essential for understanding these preferences, and the Reduced Morningness-Eveningness Questionnaire (rMEQ) has been developed for this purpose. The purpose of this study was to examine the Arabic rMEQ's psychometric properties in terms of validity, reliability and measurement invariance across sex groups. A population-based sample of 330 Arabic-speaking participants from Lebanon, comprising 67.3% females with a mean age of 21.75 ± 2.43 years (range: 18-33), were recruited for this study. The participants were asked to fill out an online questionnaire consisting of the Arabic versions of the rMEQ, Pittsburgh Sleep Quality Index (PSQI), and the Lebanese Anxiety Scale (LAS). Confirmatory factor analysis (CFA) indicated a modest fit for the one-factor model of the rMEQ. Internal reliability was good, with ω = .75 and α = .70. Additionally, cross-sex invariance analysis showed support for configural, metric, and scalar invariance, with no significant differences in rMEQ scores between males and females. Concurrent validity revealed significant moderate correlations between higher rMEQ scores and lower depression, better sleep quality, and lower anxiety. The Arabic version of the rMEQ demonstrated adequate psychometric properties in a Lebanese adult population. The CFA results support a one-factor model of the Arabic rMEQ, similarly as in the case of other language versions. Overall, the Arabic rMEQ appears to be a valid and reliable tool for evaluating morningness-eveningness in Arabic-speaking adults, with potential applications in cross-cultural chronotype studies.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"1060-1071"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring chronotype, sleep disturbances, and temperament in adolescents with attention deficit hyperactivity disorder.","authors":"İrem Cihanyurdu Erdem, Aliye Tuğba Bahadır","doi":"10.1080/07420528.2025.2511269","DOIUrl":"10.1080/07420528.2025.2511269","url":null,"abstract":"<p><p>This study aimed to explore sleep problems, chronotype, and temperament traits between adolescents with Attention Deficit Hyperactivity Disorder (ADHD) and healthy controls, and to examine how these variables relate within the ADHD group. One hundred and two adolescents with ADHD aged 11-17 recruited from Marmara University Pendik Training and Research Hospital Child and Adolescent Psychiatry Outpatient Clinic and 101 age and gender matched healthy controls participated in the study. Assessments included the Kiddie Schedule for Affective Disorders and Schizophrenia (K-SADS-PL), Junior Temperament and Character Inventory-Revised (J-TCI-R), Children's Chronotype Questionnaire (CCTQ), and Sleep Disturbance Scale for Children (SDSC). Adolescents with ADHD showed significantly higher rates of sleep disturbances and a greater prevalence of evening chronotype (63.7%) compared to controls. ADHD participants scored higher in novelty seeking and harm avoidance, while controls exhibited greater persistence and self-directedness. Notably, novelty seeking was pronounced in those with an evening chronotype and positively correlated with sleep disturbances, including difficulties initiating and maintaining sleep. Logistic regression analyses indicated that novelty seeking and age significantly predicted evening chronotype, while linear regression revealed that both novelty seeking and M/E scores predicted SDSC total scores. The findings underscore the clinical significance of addressing the interplay between chronotype, sleep disturbances, and temperament traits in adolescents with ADHD.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"879-892"},"PeriodicalIF":2.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronobiological aspects of autonomic nervous system function in Parkinson's disease: A systematic review.","authors":"Stefania Kalampokini, Antonis Pilavas, Gabriela Tsalamandris, Georgios M Hadjigeorgiou, Panagiotis Bargiotas","doi":"10.1080/07420528.2025.2515972","DOIUrl":"10.1080/07420528.2025.2515972","url":null,"abstract":"<p><p>Disruption of biological rhythms appears to play a role in Parkinson's disease (PD). We aimed to summarize findings, focusing on measurement tools and outcomes, from studies assessing dysautonomia in PD from a chronobiological perspective. We searched Pubmed and Embase in English and included 76 articles following PRISMA guidelines. Twenty-four-hour Blood Pressure Measurement (24 h ABPM) was the most popular method for assessing chronobiological changes of autonomic function in PD. Reverse blood pressure (BP) dipping, i.e. increase in nighttime systolic BP, was a common finding in PD regardless of disease stage. Most studies using 24 h ambulatory electrocardiogram (24 h ECG) recording or night polysomnography showed attenuated heart rate variability (HRV) in PD patients during both daytime and nighttime. This reduction of HRV seems to occur early in the disease course and to be more profound in more severe PD. Only a few studies used scales and/or diaries or wearable technology for assessing autonomic function in PD. Long-term monitoring methods, such as 24-h ECG and 24-h ABPM, along with advancements in wearable multisensory devices, offer promising tools for continuously tracking fluctuations of autonomic function over extended periods. These approaches provide more comprehensive insights into the autonomic dysregulation and its management in PD.</p>","PeriodicalId":10294,"journal":{"name":"Chronobiology International","volume":" ","pages":"959-974"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}