Clinical and experimental rheumatology最新文献

{"title":"Serum Wnt3A levels are significantly associated with cross-sectional vasculitis activity and end-stage kidney disease during follow-up of patients with antineutrophil cytoplasmic antibody-associated vasculitis.","authors":"Taejun Yoon, Jang Woo Ha, Yong-Beom Park, Sang-Won Lee","doi":"10.55563/clinexprheumatol/i8jnvc","DOIUrl":"10.55563/clinexprheumatol/i8jnvc","url":null,"abstract":"<p><strong>Objectives: </strong>In this study, we investigated whether serum Wnt3A levels at diagnosis reflected cross-sectional activity and predicted poor outcomes during follow-up in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV).</p><p><strong>Methods: </strong>This study included 80 patients who were newly diagnosed with AAV at a tertiary hospital. At diagnosis, whole blood was obtained from patients and sera was immediately isolated and stored at -80℃. Moreover, AAV activity was assessed using the Birmingham Vasculitis Activity Score (BVAS), and a high BVAS was defined as the highest tertile. Poor outcomes including all-cause mortality and end-stage kidney disease (ESKD) were recorded.</p><p><strong>Results: </strong>The patients had a median age of 63.5 years, with 40% being male and 60% female patients. Serum levels of Wnt3A at diagnosis were correlated with the cross-sectional BVAS and serum Wnt3A ≥411.7 pg/mL exhibited an increased risk of high BVAS. In addition, serum Wnt3A levels at diagnosis significantly correlated with cross-sectional acute-phase reactants and serum albumin levels. Furthermore, serum Wnt3A levels at diagnosis were associated with AAV exacerbation, leading to ESKD. Particularly, serum Wnt3A ≥407.1 pg/mL also demonstrated an elevated risk of ESKD (relative risk 3.867). Additionally, patients with serum Wnt3A ≥407.1 pg/mL exhibited a significantly lower cumulative ESKD-free survival rate than those with lower serum Wnt3A levels.</p><p><strong>Conclusions: </strong>This study is the first to demonstrate the clinical potential of serum Wnt3A levels at diagnosis for estimating cross-sectional activity and partially predicting the advancement to ESKD during follow-up in patients with AAV.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"674-682"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic utility of intima-media thickness measurement compared with the halo sign in temporal artery ultrasonography: a single-centre retrospective study.","authors":"Kazuma Yoshida, Ryuichi Minoda Sada, Akiharu Yoshioka, Hiroyuki Akebo, Hirofumi Miyake, Kazuhiro Hatta","doi":"10.55563/clinexprheumatol/nazkih","DOIUrl":"10.55563/clinexprheumatol/nazkih","url":null,"abstract":"<p><strong>Objectives: </strong>We aimed to examine the diagnostic utility of temporal artery ultrasonography (TAUS) based on measurement of intima-media thickness (IMT) compared with the halo sign in diagnosing cranial giant cell arteritis (GCA).</p><p><strong>Methods: </strong>We retrospectively analysed consecutive patients with clinically suspected GCA who had undergone TAUS between January 2011 and December 2021 in Tenri hospital, Japan. A cut-off value of 0.5 mm was used for the IMT of the temporal arteries. We examined the diagnostic value of TAUS based on each of the halo sign and increased IMT in diagnosing cranial GCA.</p><p><strong>Results: </strong>In total, 203 patients were included. Temporal artery biopsy (TAB) was performed in 59 patients, with 32 being biopsy-positive. Fifty-three patients were diagnosed with cranial GCA. The sensitivity and specificity of TAUS based on the increased IMT and halo sign were as follows: sensitivity, 62.3% and 32.1%; specificity, 90.0% and 100% compared with the clinical diagnosis; and sensitivity, 81.2% and 46.9%; specificity, 76.9% and 96.2% compared with the TAB. When the relationship between the IMT and halo sign was evaluated, patients with cranial GCA who presented with the halo sign had the highest IMT compared with those without the halo sign or those without cranial GCA.</p><p><strong>Conclusions: </strong>A TAUS diagnosis relying only on the halo sign is specific but can underestimate cranial GCA. Therefore, evaluation of the IMT in addition to the halo sign can improve the diagnostic accuracy of TAUS when diagnosing cranial GCA.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"661-667"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case of curative colectomy for Takayasu's arteritis.","authors":"Laura Kek, Griffin J Reed, Matthew J Koster","doi":"10.55563/clinexprheumatol/a1okxy","DOIUrl":"10.55563/clinexprheumatol/a1okxy","url":null,"abstract":"","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"769"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142496172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare presentation of granulomatosis with polyangiitis with prostate involvement.","authors":"Dilek Barutcu Atas, Harun Coban, Deniz Filinte, Arzu Velioglu, Zübeyde Serhan Tuglular, Ebru Asicioglu","doi":"10.55563/clinexprheumatol/f95u5v","DOIUrl":"10.55563/clinexprheumatol/f95u5v","url":null,"abstract":"","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"756-757"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of occipital artery ultrasound to diagnose and monitor response to therapy in a patient with giant cell arteritis.","authors":"Daniel Montes, John J Schmitz, Tawatchai Paisansinsup, Kenneth J Warrington, Matthew J Koster","doi":"10.55563/clinexprheumatol/8kum8n","DOIUrl":"10.55563/clinexprheumatol/8kum8n","url":null,"abstract":"","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"762-763"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated serum uric acid is a predictor of pulmonary artery involvement and subsequent prognosis in patients with Takayasu's arteritis.","authors":"Hua Liao, Shiyu Yang, Nan Zhang, Juan Du, Hui Yuan, Lili Pan","doi":"10.55563/clinexprheumatol/cv6z35","DOIUrl":"10.55563/clinexprheumatol/cv6z35","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to investigate the predictive value of uric acid (UA) in prognosis of pulmonary artery involvement (PAI) in patients with Takayasu's arteritis (TAK).</p><p><strong>Methods: </strong>A total of 166 TAK patients were enrolled in the study, including 76 with PAI and 90 without. Outcomes of 144 TAK patients were followed up and recorded. The possible associations between serum UA levels and incidence of PAI in TAK and PAI-related prognosis of TAK patients were examined using different statistical models.</p><p><strong>Results: </strong>The serum UA levels were significantly higher in TAK patient with PAI than TAK patients without PAI. Multivariate logistic regression analysis indicated that serum UA level ≥284.5 umol/L was associated with an increasing incidence of PAI in TAK (OR: 2.108, 95% CI: 1.063 to 4.180; p=0.033). Kaplan-Meier survival analysis showed that TAK patients with serum UA level ≥328.1 umol/L had a significantly higher cumulative incidence of PAI-related adverse events compared to TAK patients with serum UA level <328.1 umol/L (p=0.008). Multivariate Cox proportional hazard regression analysis revealed that serum UA level ≥328.1 umol/L (HR: 2.595, 95% CI: 1.198 to 5.622; p=0.016) was a PAI-related prognostic risk factor for TAK.</p><p><strong>Conclusions: </strong>Elevation of serum UA level was associated with an increasing risk of PAI and PAI-related adverse event in patients with TAK, indicating its potential as a predictor for identification of PAI onset and worsening in TAK patients.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"602-610"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granzyme B producing regulatory B-cells in patients with giant cell arteritis.","authors":"Marian Stöcker, Uta Kiltz, Jürgen Braun, Xenofon Baraliakos, Xin Ma, Dai Yang, Andreas Kribben, Benjamin Wilde","doi":"10.55563/clinexprheumatol/37o5o5","DOIUrl":"10.55563/clinexprheumatol/37o5o5","url":null,"abstract":"<p><strong>Objectives: </strong>Giant cell arteritis (GCA) is a serious inflammatory rheumatic disease driven by T-cells which are regulated by B-cells with anti-inflammatory activity. However, the role of these regulatory B-cells has not been studied in detail. The aim of this study is to investigate the anti-inflammatory B-cell compartment in patients with GCA.</p><p><strong>Methods: </strong>Peripheral blood mononuclear cells (PBMC) of GCA Patients (n=47) and healthy controls (HC) (n=49) were isolated to assess the granzyme B (GrB) and Interleukin- 10 (IL-10) production of regulatory B-cells (Breg) after in vitro stimulation.</p><p><strong>Results: </strong>The fraction of GrB producing Breg in GCA patients was diminished as compared to HC, and this was independent of current disease activity. In contrast, there were no significant differences between patients and HC with regard to IL-10 producing Breg. In GCA patients with active disease, CD4+ T-cells produced less IFNγ than HC. Regarding other T-cell derived pro-inflammatory cytokines, a trend towards a lower expression as compared to HC was seen.</p><p><strong>Conclusions: </strong>Regulatory B-cells were differentially altered in patients with GCA. While GrB producing Breg were persistently diminished, IL-10 producing Breg showed no differences between patients and controls. This may indicate a lack of B-cell based suppressive capacity which has influence on the T-cell compartment.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"655-660"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Memoriam of Alfonse T. Masi.","authors":"Maurizio Cutolo, Stefano Bombardieri","doi":"10.55563/clinexprheumatol/1rlbck","DOIUrl":"10.55563/clinexprheumatol/1rlbck","url":null,"abstract":"","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":"0"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D label-free quantitative proteomic and phosphoproteomic profiling of the kidney in rat models of antimyeloperoxidase-associated vasculitis.","authors":"Congcong Zhao, Hongli Xiong, Zhe Zheng, Li Zhang, Cunhao Bian, Minzhu Zhao, Yongguo Li, Jianbo Li","doi":"10.55563/clinexprheumatol/880sth","DOIUrl":"https://doi.org/10.55563/clinexprheumatol/880sth","url":null,"abstract":"<p><strong>Objectives: </strong>The early diagnosis of antimyeloperoxidase (anti-MPO) -associated vasculitis is still a challenge in clinical practice. This study aimed to facilitate the early diagnosis of anti-MPO-associated vasculitis and investigate its pathogenesis.</p><p><strong>Methods: </strong>We established an animal model of experimental autoimmune vasculitis (EAV), and applied 4D label-free technology to identify proteins and phosphorylated proteins in the kidneys of the EAV group and control group.</p><p><strong>Results: </strong>A total of 674 differentially expressed proteins (DEPs) were identified by proteomics, including 347 up-regulated and 327 down-regulated proteins. The results of the GO and KEGG enrichment analysis showed that DEPs were mainly involved in complement and coagulation cascade reactions, as well as the formation of neutrophil extracellular traps. According to the analysis of MCODE and hub proteins, FGG, HRG, C3, SERPIND1, ITIH2, A2M were selected as target proteins, and parallel reaction monitoring (PRM) was used to validate the above proteins. The validation results were consistent with the proteomic detection results. Through de-background analysis of differentially expressed phosphorylated proteins, a total of 497 differentially expressed phosphorylated attributed proteins were identified, 256 of which were down-regulated and 241 were up-regulated. These proteins mainly participated in energy metabolism-related pathways. The prediction analysis of upstream phosphorylated kinases suggested that kinases with significantly up-regulated activity included CAMK2D, PAK2, CAMK2B, and PRKAA2, while kinases with down-regulated activity included ADRBK1 and PLK1.</p><p><strong>Conclusions: </strong>The results of this study could provide some auxiliary indicators for the early diagnosis of anti-MPO-associated vasculitis, and provide a basis for further understanding of its pathogenesis.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":"43 4","pages":"683-693"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxychloroquine inhibits the PI3K/AKT pathway in synovial fibroblasts of rheumatoid arthritis and alleviates collagen-induced arthritis in mice.","authors":"Xiaocheng Wang, Jiaxin He, Jianqiu Zhong, Juan He, Deli Wang, Lu Bai, Hongxi Shang, Qingwen Wang","doi":"10.55563/clinexprheumatol/tczujg","DOIUrl":"https://doi.org/10.55563/clinexprheumatol/tczujg","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of hydroxychloroquine (HCQ) in rheumatoid arthritis (RA), particularly on fibroblast-like synoviocytes (FLS).</p><p><strong>Methods: </strong>We analysed the R (-) and S (+) enantiomers of HCQ. Cell viability and proliferation inhibition were quantified using CCK-8 and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively. Flow cytometry was employed to analyse cell cycle arrest and apoptosis induction. Transwell assays were conducted to evaluate cellular motility. Molecular docking simulations with key targets predicted binding interactions. The therapeutic efficacy was validated in a collagen-induced arthritis (CIA) mouse model.</p><p><strong>Results: </strong>The study finds that these enantiomers in a racemic combination, known as Rac-HCQ, are potent in arresting the growth of RA-FLS by inhibiting cell migration and invasion through downregulation of vimentin expression, inducing apoptosis via increased bax expression, and promoting cell cycle arrest by suppressing CDK1, cyclins A2, and B1. In vivo experiments showed that Rac-HCQ significantly reduced symptom severity in collagen-induced arthritis (CIA) mice. RNA sequencing suggests that Rac-HCQ alters the disease pathway in RA-FLS by blocking the PI3K/AKT pathway.</p><p><strong>Conclusions: </strong>Our molecular docking studies indicate KIT, SRC, and PIK3A may potentially serve as the targets of Rac-HCQ in RA. These findings reveal the potential of Rac-HCQ to modulate the functions of RA-FLS and mitigate CIA manifestations, principally through its interaction with the pathway PI3K/AKT.</p>","PeriodicalId":10274,"journal":{"name":"Clinical and experimental rheumatology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}